Method used for seed selection of sorghum sterile lines

A technology for sterile lines and varieties, applied in the field of crop genetics and breeding, can solve the problems such as the difficulty of improving the yield and disease resistance of hybrids, the limited genetic foundation of sterile lines, and the difficulty in breaking through the utilization mode of hybrid advantages, etc. The stability of hereditary traits is strong, the plants are short, and the effect of expanding the scope of resource utilization

Inactive Publication Date: 2017-09-22
JILIN ACAD OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The genetic basis of the sterile line selected by this method is limited, and it is difficult to break through the utiliz

Method used

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  • Method used for seed selection of sorghum sterile lines

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Comparison scheme
Effect test

Embodiment 1

[0040] Example 1, Breeding Sorghum CMS Line Ji 2060A

[0041] In this example, the hybridization of the maintainer line and the restorer line is used, and then backcrossing is used to select the sterile line Sorghum Ji 2060A. The specific breeding process is as follows: figure 1 shown.

[0042] Such as figure 1 As shown, in 2005, the maintainer line QL33B was used as the female parent, and the restorer line Jihui 13 (Feiteruite×Xizangbai×Hei 30B) was used as the male parent through artificial emasculation and self-crossing for 2 consecutive generations. 3 Among them, select a single plant with short plant height (60cm), compact plant type, good comprehensive traits, and early growth period to backcross with QL33B, and then continuously self-cross for three generations. 1 f 4 Among them, a single plant with short plant height, compact plant type and early growth period was selected to be backcrossed with the sterile line QL33A to produce a sterile line. After 6 generations o...

Embodiment 2

[0044] Example 2, Trait Advantages of Sorghum CMS Line Ji 2060A

[0045] The Ji 2060A male sterile line bred by the above-mentioned embodiment has a growth period of 110 days, a plant height of 60 cm, and a compact and uniform plant type; the planting density is 25-30 plants per square meter, the yield per mu is 400 kg, and the seed production yield is high. .

[0046] From the above experimental results, it can be seen that compared with other sterile lines, the sterile line Ji 2060A selected by the method for breeding sorghum sterile lines provided by the present invention has a short growth period, short plants, and attainable The degree of secret planting is high, the yield per mu is high, and the stability of genetic traits is strong.

Embodiment 3

[0047] Example 3. Comparison test of cold tolerance in germination stage of sorghum male sterile line Ji 2060A

[0048] 75 seeds with the same size and full grains were selected for each material. Disinfect with 1g / L mercuric chloride solution for 10 minutes, then rinse with tap water for 3 times and deionized water for 3 times. Arrange evenly and neatly in a 9cm petri dish with a cover covered with 2 layers of filter paper, add 8ml of water, place 3 petri dishes for each material, and 25 seeds in each incubator. Put them into an incubator at a temperature of 4°C and culture them in the dark for 5 days. On the 6th day, the temperature was increased to 15°C, and the time required for germination and the germination rate were observed. The results showed that the germination time of Ji 2060A was 5 days, and the germination rate was 80%, and the germination time of 4190A was 8 days, and the germination rate was 50%.

[0049] From the above experimental results, it can be seen ...

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Abstract

The invention discloses a method for breeding a sorghum sterile line, the method comprising: using a maintainer line and a restorer line to cross to breed a sterile line; the method comprises using the maintainer line QL33B as the female parent, and the restorer line Hui 13 is the male parent, crossed by artificial emasculation, and self-crossed continuously for 2 generations. In F3, a single plant with good comprehensive traits such as short plant height, compact plant type, and early growth period was selected to backcross with QL33B, and then continuously self-crossed for 3 In BC1F4, a single plant with short plant height, compact plant type and early growth period was selected to be backcrossed with QL33A to produce a sterile line. After 6 generations of continuous backcrossing, a stable sterile line Ji 2060A was transformed. The Ji 2060A bred by using the method for breeding a sorghum male sterile line provided by the present invention has a short growth period, a short plant, a large degree of secret planting that can be achieved by the plant, and a high yield per mu. The method for breeding sorghum sterile lines provided by the invention broadens the blood relationship of the existing sterile lines and improves the adaptability of the sterile lines.

Description

technical field [0001] The invention belongs to the field of crop genetics and breeding, and in particular relates to a method for breeding sorghum sterile lines. Background technique [0002] There are generally two kinds of existing sorghum male sterile line breeding methods, one is to introduce male sterile lines and directly use combined hybrids; Good single plants were selected for backcrossing. The genetic basis of the sterile line selected by this method is limited, and it is difficult to break through the utilization mode of hybrid vigor, and it is difficult to improve the advantages of hybrids such as yield and disease resistance. [0003] In order to broaden the genetic basis of germplasm, expand the scope of resource utilization, change the utilization mode of heterosis, and further improve the yield, disease resistance and quality of sorghum, it is necessary to break the existing breeding ideas. Contents of the invention [0004] The present invention breaks ...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 陈冰嬬高士杰李继洪侯佳明高明超徐宁李伟
Owner JILIN ACAD OF AGRI SCI
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