82 results about "Consanguinity" patented technology

The present invention relates to a neo4j-based big data consanguinity management method, system, apparatus and storage medium. The method comprises the following steps of parsing SQL statements to generate a corresponding abstract syntax tree; for each abstract syntax tree, traversing each node of the abstract syntax tree in depth, and collecting the corresponding node data at each node; storing the collected node data relationship in neo4j graphic database, and storing the collected necessary information in HBase; introducing the information from heterogeneous data sources into consanguinitysystem to form consanguinity. The method is easy to graphically display the dependencies and consanguinity between data sources and Hive tables, enhances the level of metadata management capability ofbig data platform, strengthens the control of data flow within the platform, sorts out the origin and development of data, breaks through the estrangement of heterogeneous data sources, and connectsthe data sources as a bridge of consanguinity.

The invention relates to a poultry breeding technology, in particular to a cultivation method of high-yield native laying chicken. The cultivation method comprises the following steps of: F-series cultivation, backcrossing royal chicken with the synthesis series of parental royal chicken and parental Loman laying chicken, obtaining an underlying group strain containing 75 percent of royal chicken consanguinity, and then carrying out continuous four-generation selective breeding; M-strain breeding cultivation: carrying out cross breeding on the parental royal chicken and the parental Loman laying chicken to obtain an underlying group strain containing 50 percent of the royal chicken consanguinity, and then carrying out continuous four-generation selective breeding; producing commercial generation in matching strains: by taking F strains as parental generation male parent and M strains as parental generation female parent, carrying out sex determination on filial generation by adopting an anal swelling method, wherein the female chicken are commercial-generation laying chicken. The cultivation method can cultivate new species (matching strains) of native laying chicken with high egg yield and excellent egg quality, the egg yield of one commercial chicken after entering into a pheasantry for 72 weeks reaches more than 235 and is improved by 50 percent compared with the egg yield of pure native laying chicken; and the commercial eggs have same appearance quality, protein quality and flavor characteristics with native eggs.

The invention provides a small interference RNA molecule SiRNA capable of attacking human hepatitis B virus, which is double chain RNA molecule whose sequence has at least 70% of consanguinity degree with the sequence (I), wherein the antisense chain of the sequence (I) and one nucleic acid mutant has no consanguinity with the known human gene and gene expression segment. The SiRNA can be used for preparing medicament or preparation for prevention or treating hepatitis B and any diseases relating to hepatitis B viral infection.

The invention discloses a breeding method of drought resistant and cold tolerant Saccharum varieties/lines containing Erianthus fulvus Ness consanguinity. The method provided in the invention makes use of Erianthus fulvus Ness wild species (E. fulvus) and Saccharum cultivars (Saccharum spp.) to perform "interspecific distant hybridization". The hybrid progenies undergo selection from a seedling stage, i.e. under conditions (dryland, high altitude) with natural environmental stress, thus obtaining new drought resistant and cold tolerant Saccharum varieties/lines suitable to plant at an altitude of over 1300 meters.

The invention discloses an electric power customer service work order sentiment quantitative analysis method based on Word2Vec, and relates to an electric power customer service work order analysis method. A traditional sentiment analysis method cannot effectively discriminate the sentiment intensity. The method of the invention comprises the steps of combining the power customer service work order text features; classifying and sorting the historical electric power customer service work orders and the unsatisfied work orders, cleaning data, combing based on the Baidu word bank to form an initialized multivariate emotion word bank; carrying out the work order text word segmentation by adopting a reverse maximum matching algorithm; based on the Word2Vec neural network, constructing the positive words, negative words, degree adverbs and a word vector of a word order fused with customer appeal semantics; performing the machine learning training through the historical customer service workorder to generate a learning model fusing appeal emotion, expanding a part-of-speech corpus based on the part-of-speech affinity-consanguinity relationship in the model, performing emotion quantization calculation by adopting a similarity word sequence matrix quantization algorithm, and completing customer service work order emotion quantization analysis, thereby effectively distinguishing emotion intensity differences, and determining an emergency degree.

The invention discloses an amino acid sequence of beta-mannase gene and coding making method in the biological domain, which is characterized by the following: extracting Armillariella tabescens main RNA of high activity by konjaku flour evoking; using consanguinity sequence of Genebank to design degenerate primer; carrying on PCR expanding to protectorate by primer; designing gene specificity primer to carry on 3'RACE and 5'RACE; cloning total long cDNA of beta-mannase gene; constructing eucaryon expressing carrier with pichia; building-up yeast gene engineering bacterial strain.

The invention relates to an animal cultivation method, in particular to a cultivation method for a green-shelled-egg chicken pure line. The method adopts Jiuyuan black chickens as materials, and the method can be applied to purification of varieties of other chickens with green-shelled-egg consanguinity. The method comprises the following steps: 1) selection of parents; 2) production of homozygosis green shell gene individuals; 3) expanding propagation of colony. The method has the advantages that the construction of green-shelled-egg chicken homozygosis colony is realized, homozygosis genotype green-shelled-egg chickens can be bred by screening heterozygosis genotype at the natural conditions and adopting a scientific breeding scheme, quick expanding propagation can be realized, and the method can be applied to purification of varieties of other chickens with green-shelled-egg consanguinity.

The invention relates to thirteen new genes of bzip transcription factor expressed in the shelled corn growth process and their sequence analysis and expression analysis. The name of bzip transcription factor gene is MSBZIP1-MSBZIP13, and the nucleic acid sequence is one of the below: (1)1-13 DNA sequence in the sequence table, (2)1-13 limited DNA sequence with more than 90% consanguinity in the sequence table and DNA sequence with the same coding functional protein. The protein which is coded by bzip transcription factor gene has 14-26 amino acid residue sequence in the sequence table or has the same active 14-16 derived protein sequence with 14-26 amino acid residue sequence by replacing, missing or adding 14-26 amino acid residue sequence. Thirteen new genes of bzip transcription factor in the core is reported firstly, which provides the therapy for analyzing the expression and control of the gene in the shelled corn growth process and utilizing these transcription factors.

The invention belongs to the technical field of biological genetics, and relates to a probability statistics model-based biological full-sibling identification method. According to the method, STR locus genotyping is performed on two identified persons so as to obtain the genotyping results of n mutually independent STR loci; and a probability pH0 that the two identified persons are non-consanguinity relation individuals and a probability pH1 that the two identified persons are full siblings can be calculated according to the binomial distribution IBS-B (2n,pi0) of IBSs (identity by states) in a non-consanguinity relation individual pair group and the binomial distribution IBS-B (2n,pi1) of the IBSs in a full-sibling pair group. The probability statistics model-based biological full-sibling identification method of the invention is applicable to any number of STR loci, and has high analysis efficiency, and therefore, the method provided by the technical schemes of the invention has the advantages of broad application prospect and good market potential.

The invention discloses a high-throughput STR typing system capable of identifying complex consanguinity and a kit thereof, and relates to the technical field of nucleic acid detection in vitro. The high-throughput STR typing system capable of identifying complex consanguinity comprises PCR primers used for amplifying 60, 118 or 179 linked autosomal STR loci; and the kit comprises a PCR primer composition, an Index connector sequence, an IGT-EM707 polymerase mixture, an amplification buffer enhancer NB, a YF buffer solution B, a database setting-up reagent and the like. The high-throughput STRtyping system capable of identifying complex consanguinity and the kit thereof provided by the invention are capable of realizing single-tube amplification of 60, 118 or 179 linked autosomal STR loci; and moreover, the STR typing system and the kit thereof are good in balance, high in sensitivity, good in specificity, and accurate in typing result. Thus, the high-throughput STR typing system capable of identifying complex consanguinity and the kit thereof can be used for identifying complex relationships of different consanguinity at the same level.

The invention relates to the field of crop cultivation, in particular to a purple cauliflower hybrid seed cultivation method. A mediummorchid parent 97.f3-7-3-1a3 is taken as a main body parent to be respectively hybridized with purple-flower vegetable material ZiYin-1 with Europe consanguinity, variant distant related ZiPa (purple cabbage) according to a seed cultivation object design technical scheme by use of existing germplasm resources; backcross or continuous backcross is performed on the F1 generation, individual selection and autocopuation in a net chamber are performed BC1, BC2 and descendants by virtue of a pedigreen method according to the seed cultivation object, so that two stable-character and good-regularity self-incompatible lines are line-bred after 6-7 generations; the hybrid F1 generation has great heterosis and has yield which is increased by not less than 80% than those of parents, and ball flowers are purple red in color, compact, gorgeous and glossy, taste crisp, tender and sweet if being not cooked, taste delicious, tasty and refreshing if being cooked, and are named as ZIJING-1.

The invention T.chinensis taxane C13-side chain-N-benzoyl-transporting proteinase coding sequence, and the separated DNA molecule includes: a nucleotide sequence coding polypeptide with the activity of C13-side chain-N-benzoyl-transporting proteinase, where the nucleotide sequence has at least 70% consanguinity with that (36-1352 nt) in SEQ ID No.3; or the nucleotide sequence can hybridize with that (36-1352 nt) in SEQ ID No.3 at 40-55 deg.C. The invention is an enzyme catalyzing benzoyl CoA and debenzoyl-taxol to generate taxol, and with good help to the health of people.

The invention discloses a method for breeding a sorghum sterile line, the method comprising: using a maintainer line and a restorer line to cross to breed a sterile line; the method comprises using the maintainer line QL33B as the female parent, and the restorer line Hui 13 is the male parent, crossed by artificial emasculation, and self-crossed continuously for 2 generations. In F3, a single plant with good comprehensive traits such as short plant height, compact plant type, and early growth period was selected to backcross with QL33B, and then continuously self-crossed for 3 In BC1F4, a single plant with short plant height, compact plant type and early growth period was selected to be backcrossed with QL33A to produce a sterile line. After 6 generations of continuous backcrossing, a stable sterile line Ji 2060A was transformed. The Ji 2060A bred by using the method for breeding a sorghum male sterile line provided by the present invention has a short growth period, a short plant, a large degree of secret planting that can be achieved by the plant, and a high yield per mu. The method for breeding sorghum sterile lines provided by the invention broadens the blood relationship of the existing sterile lines and improves the adaptability of the sterile lines.

The invention relates to the field of animal breeding, and particularly discloses a new Tibetan pig strain cultivation method. A specific Tibetan pig selection indicator is formulated according to the characteristics of the Tibetan pig variety, and two selection index calculation equations are formulated according to the breeding target. Tibetan pigs with different consanguinities are taken as the basic group zero generation, and the first-generation pig group is selected and reserved according to the 25 kg weight day age breeding value, the backfat thickness breeding value and the litter size breeding value. Selection is conducted in two directions starting from the second-generation pig group, continuous breeding of three to four generations is conducted according to the formulated paternal line selection index and maternal line selection index, and then the new Tibetan pig strain I (fast growth strain) and II (high prolificacy strain) are obtained. High-quality and high-yield new Tibetan pig strain commercial pigs can be produced with the strain II serving as the female parent and the strain I serving as the male parent. New strain pigs have the advantages of being high in growth speed, large in litter size, high in meat quality, high in cold resistance and stress resistance and the like.

The invention discloses a fast balance breeding method for native hen varieties, and belongs to the technical field of poultry breeding. According to the method, a hen strain with 50-percent high-egg-laying consanguinity is subjected to hybridizing matching with the hen strain with 75-percent high-egg-laying consanguinity to obtain a high-quality hen strain with 62.5-percent high-egg-laying consanguinity, the genetic negative correlation properties between the egg quality and the high-egg-laying properties can be well balanced through the high-egg-laying consanguinity contents of the matching strain, the egg laying properties are improved, meanwhile, the inherent egg quality of the native hens are maintained to the maximum degree, and the optimum high yield and high quality are realized. The progenitor male parent or female parent pure strains adopting high-yield hen variety matching combination by external consanguinity are imported, the genotype homogeneity can be favorably accelerated, the matching power of the matching strain is improved, meanwhile, a molecular marker auxiliary selection technology is adopted, the early stage selection is carried out, the generation interval is shortened, and the breeding process is accelerated.

The invention discloses a control gene OsCM2 separating and cloning method and application of rice ear sprouting period, which is characterized by the following: possessing 50% consanguinity for protein coded by OsCM2 gene and CM-2 gene; controlling the ear sprouting period through improving or weakening OsCM2 gene; improving the adaptability of region and season.

Disclosed is a recombined chicken pox virus vaccine rFPV-12LSH5H9, its construction method and use, wherein the construction process consists of placing AIV HA gene fragment containing H5 hypotype and H9 hypotype onto the downstream of right promotors, inserting into chicken poxviridae expression vector p12-18 containing FPV duplicated non-indispensable fragment FPV1-2 and reported genes through backside cascade connection to construct transition vector p12LSH5H9, then transforming chick embryo desmocyte infected with wt-FPV with the transition vector p12LSH5H9, and obtaining co-expression H5 and H9 hypotype AIV HA gene rFPV-12LSH5H9 through consanguinity recombination.

The invention provides a method for detecting Psa in kiwi fruit pollens. The method comprises the following steps: extracting DNA from pollen mixed bacterial suspension; designing two pairs of specific primers according to hrpW gene of kiwi fruit Psa, carrying out nested-PCR, amplifying a pollen sample to obtain an outer primer amplified band with a size of about 590 bp and an inner primer amplified band with a size of about 240 bp; and finally carrying out sequencing to verify the Psa. The detection sensitivity is 103 cfu/mL. The provided Psa rapid detection method has the advantages of stronger specificity, higher stability, and higher sensitivity, can differentiate pathogenic bacteria having close ties of consanguinity with Psa such as pseudomonas syringae pv.Theae, pseudomonas avellanae, and the like, and is capable of reducing the false positive results.

The invention relates to a method for identifying consanguinity of saccharum arundinaceum in sugarcane via a spread sequence. The method comprises steps of preparing a root-tip cell slide, preparing alabeled probe, pre-processing the root-tip slide, preparing a denaturing hybrid mixed solution and hybridizing the denaturing hybrid mixed solution and the pre-processed root-tip slide. A to-be-detected sample is judged that the sample contains the consanguinity of saccharum arundinaceum and is a real hybrid later generation of sugarcane and saccharum arundinaceum when a green fluorescence signalis generated on the hybrid slide. The nucleotide sequence of a probe which generates the green fluorescence signal is shown as SEQ ID NO:1 in a sequence list. The method provided by the invention, through the combination with fluorescence in-situ hybridization technology and by identifying the real hybrid later generation of sugarcane and saccharum arundinaceum through the spread sequence probe that saccharum arundinaceum is specific to sugarcane, has the characteristics of being high in efficiency, good in sensitivity, good in stability and high in reliability; and the reality of any hybridgenerations can be detected just by acquiring root tips of sugarcane seedlings, so that an efficient and stable identifying method is provided for hybridization utilization of the saccharum arundinaceum in a sugarcane genetic breeding improving process.