Golden snub-nosed monkey inheritance monitoring and breeding management method based on SNP site

A golden monkey and site technology, applied in the field of genetic diversity monitoring and family breeding management, can solve the problems of population genetic diversity loss, affecting the long-term development of the population, and the decline of offspring viability, achieving high mutation rate, easy data analysis, reliable results
CN107779499AInactive Publication Date: 2018-03-09INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
Publication Date
2018-03-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a golden snub-nosed monkey individual inheritance diversity monitoring method. The method includes steps of (1), collecting a golden snub-nosed monkey blood sample, and extracting gene group DNA of the blood sample; (2), performing gene group DNA sequencing by means of RAD-seq; (3), analyzing to obtain the SNP site; (4), performing genetic variation monitoring of population; (5), selecting the SNP site, and building a dynamic family relationship of a breeding group on the basis of the ties of consanguinity. By means of the acquired SNP site, the golden snub-nosed monkeyindividual inheritance diversity monitoring method can simply, exactly and comprehensively acquire the inheritance information and ties of consanguinity of the golden snub-nosed monkey individual, and is good for monitoring the inheritance diversity of the golden snub-nosed monkey breeding group for a long time and building up a dynamic breeding family pedigree. Compared with the existing inheritance marking method, the SNP site is high in mutation rate, large in site amount, and wide in distribution; more comprehensive inheritance information can be acquired, the result is more reliable; compared with a full gene group sequencing, the SNP site is simple, and data is easy to analyze.
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Description

technical field

[0001] The invention relates to genetic diversity monitoring and family breeding management of Sichuan snub-nosed monkeys, in particular to methods for genetic diversity monitoring and family breeding management based on SNP sites. Background technique

[0002] Sichuan snub-nosed monkey (Rhinopithecus roxellana), belonging to primates, monkeys, colobus subfamily, snub-nosed monkey genus, is a unique species in my country, a national first-class key protected wild animal, and is recognized by the International Union for Conservation of Nature (International Union for Conservation of Nature) , IUCN) list as Vulnerable (IUCN, 2011). At present, Sichuan snub-nosed monkeys are only distributed in several isolated areas (northern Sichuan and southern Gansu, Qinling in Shaanxi, and Shennongjia, Hubei). Among them, the population of Shennongjia golden monkeys is an isolated group of golden monkeys distributed in the easternmost part. In the protection and research of ...

Claims

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