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Golden snub-nosed monkey inheritance monitoring and breeding management method based on SNP site

A golden monkey and site technology, applied in the field of genetic diversity monitoring and family breeding management, can solve the problems of population genetic diversity loss, affecting the long-term development of the population, and the decline of offspring viability, achieving high mutation rate, easy data analysis, reliable results

Inactive Publication Date: 2018-03-09
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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Problems solved by technology

Inbreeding can lead to inbreeding depression such as reduced offspring viability, reduced reproductive fitness, and high mortality, and it can also lead to a rapid loss of population genetic diversity, thereby affecting the long-term development of the population

Method used

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  • Golden snub-nosed monkey inheritance monitoring and breeding management method based on SNP site
  • Golden snub-nosed monkey inheritance monitoring and breeding management method based on SNP site
  • Golden snub-nosed monkey inheritance monitoring and breeding management method based on SNP site

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Embodiment Construction

[0043] The present invention will be further clarified through the detailed description of specific embodiments below, but it is not intended to limit the present invention, but only for illustration.

[0044] (1) Material

[0045] The blood samples of Sichuan snub-nosed monkeys were collected, and all samples were cryopreserved in sterilized test tubes containing anticoagulants.

[0046] (2) Extraction of genomic DNA from blood samples

[0047] DNA extraction from blood samples using Blood DNA Mini Kit (QIAGEN) kit. The extraction method refers to the kit instruction manual, and the specific operation is as follows:

[0048] 1) Take 200 μl of dissolved blood sample into a new 2ml centrifuge tube, add 20 μl of proteinase K to the centrifuge tube with the sample;

[0049] 2) Take 200 μl AL buffer solution into a centrifuge tube, mix well, and incubate in a 56°C water bath for 30mins;

[0050] 3) Add 200 μl of absolute ethanol into the incubated solution, mix well, and cen...

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Abstract

The invention discloses a golden snub-nosed monkey individual inheritance diversity monitoring method. The method includes steps of (1), collecting a golden snub-nosed monkey blood sample, and extracting gene group DNA of the blood sample; (2), performing gene group DNA sequencing by means of RAD-seq; (3), analyzing to obtain the SNP site; (4), performing genetic variation monitoring of population; (5), selecting the SNP site, and building a dynamic family relationship of a breeding group on the basis of the ties of consanguinity. By means of the acquired SNP site, the golden snub-nosed monkeyindividual inheritance diversity monitoring method can simply, exactly and comprehensively acquire the inheritance information and ties of consanguinity of the golden snub-nosed monkey individual, and is good for monitoring the inheritance diversity of the golden snub-nosed monkey breeding group for a long time and building up a dynamic breeding family pedigree. Compared with the existing inheritance marking method, the SNP site is high in mutation rate, large in site amount, and wide in distribution; more comprehensive inheritance information can be acquired, the result is more reliable; compared with a full gene group sequencing, the SNP site is simple, and data is easy to analyze.

Description

technical field [0001] The invention relates to genetic diversity monitoring and family breeding management of Sichuan snub-nosed monkeys, in particular to methods for genetic diversity monitoring and family breeding management based on SNP sites. Background technique [0002] Sichuan snub-nosed monkey (Rhinopithecus roxellana), belonging to primates, monkeys, colobus subfamily, snub-nosed monkey genus, is a unique species in my country, a national first-class key protected wild animal, and is recognized by the International Union for Conservation of Nature (International Union for Conservation of Nature) , IUCN) list as Vulnerable (IUCN, 2011). At present, Sichuan snub-nosed monkeys are only distributed in several isolated areas (northern Sichuan and southern Gansu, Qinling in Shaanxi, and Shennongjia, Hubei). Among them, the population of Shennongjia golden monkeys is an isolated group of golden monkeys distributed in the easternmost part. In the protection and research of ...

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Application Information

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IPC IPC(8): C12Q1/6869G06F19/22
CPCC12Q1/6869G16B30/00C12Q2521/313C12Q2525/191
Inventor 张于光张宇李迪强
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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