Breeding method for colored-leaf-variety of Portulacaria afra

A technology of golden branches and jade leaves and colorful leaves, which is applied in the field of plant tissue culture, can solve the problems that materials are easily polluted by the outside world, the lethality rate increases, and the effect is not ideal, so as to improve the mutation rate and transplant survival rate, shorten the breeding period, reduce the Effects of Mutation Type Limits

Inactive Publication Date: 2017-06-30
河南红枫生物高科股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the spheroids or clustered buds are irradiated and then transferred to the culture medium, the materials are easily polluted by the outside world, and the lethality rate increases
[0006] However, there is no report in the prior art on the use of radiation breeding technology to bree

Method used

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  • Breeding method for colored-leaf-variety of Portulacaria afra
  • Breeding method for colored-leaf-variety of Portulacaria afra

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0036] Example 1 Breeding method of golden branch jade leaf color leaf variety of the present invention

[0037] 1. Obtain the tissue culture seedlings of golden branches and jade leaves

[0038] The plump young stems from the vigorously growing golden branches and jade leaves that year were inoculated to contain 0.5mg / L 6-BA, 0.05mg / LNAA, 5mg after disinfection and sterilization with 0.1% sodium hypochlorite, 75% alcohol, and 0.1% mercury. / L SNP, 0.8g / L colchicine, 30g / L sucrose and 7g / L agar MS medium for primary culture, followed by subculture and rooting culture, the secondary culture medium contains 0.6mg / L MS medium with 6-BA, 0.05mg / L NAA, 4mg / L SNP, 25g / L sucrose and 7.5g / L agar, pH 5.9, rooting medium containing 0.4mg / L 6-BA, 0.03mg / L NAA, 4mg / L SNP, 25g / L sucrose and 7.5g / L agar in 1 / 2MS medium, pH 5.9; rooting culture for 10-15 days to obtain golden twig jade leaf tissue culture seedlings.

[0039] In the experiment, a control plant was set. The control plant was not a...

Example Embodiment

[0054] Example 2 Breeding method of golden branch jade leaf color leaf variety of the present invention

[0055] The plump young stems from the vigorously growing golden branches and jade leaves that year were inoculated to contain 0.5mg / L 6-BA, 0.05mg / LNAA, 5mg after disinfection and sterilization with 0.1% sodium hypochlorite, 75% alcohol, and 0.1% mercury. / L SNP, 0.5g / L colchicine, 30g / L sucrose and 7g / L agar on MS medium. After three stages of primary culture, secondary culture and rooting culture (the medium for secondary culture and rooting culture is the same as in Example 1)). Take roots and cultivate for 10-15 days to obtain tissue culture seedlings of golden branches and jade leaves.

[0056] The radiation treatment of the golden twig jade leaf tissue culture seedlings, the transplanting and observation of the radiation tissue culture seedlings, the screening and propagation methods of the variant plants are the same as those in Example 1.

Example Embodiment

[0057] Example 3 Breeding method of golden branch jade leaf color leaf variety of the present invention

[0058] The plump young stems from the vigorously growing golden branches and jade leaves that year were inoculated to contain 0.5mg / L 6-BA, 0.05mg / LNAA, 5mg after disinfection and sterilization with 0.1% sodium hypochlorite, 75% alcohol, and 0.1% mercury. / L SNP, 1g / L colchicine, 30g / L sucrose and 7g / L agar on MS medium. After three stages of primary culture, secondary culture and rooting culture (subculture and rooting culture medium are the same as in Example 1). Take roots and cultivate for 10-15 days to obtain tissue culture seedlings of golden branches and leaves.

[0059] The radiation treatment of the golden twig jade leaf tissue culture seedlings, the transplanting and observation of the radiation tissue culture seedlings, the screening and propagation methods of the variant plants are the same as those in Example 1.

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Abstract

The invention relates to the technical field of plant tissue culture, in particular to a breeding method of a golden branch jade leaf color leaf variety. The present invention combines tissue culture technology with two mutagenesis methods of colchicine chemical mutagenesis and radiation induction, which significantly improves the mutation rate of golden branch and jade leaves, shortens the breeding period, expands the variation range of plants at the same time, and reduces the impact of parents on The limitation of variation types provides a simple and efficient method for the breeding of new varieties of Jinzhiyuyecai, which enriches the planting resources of Euonymus.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a breeding method of a golden branch jade leaf color leaf variety. Background technique [0002] Jinzhiyuye is a variety of Euonymus genus silk floss, a new variety of Euonymus developed by Henan Hongfeng Seedling Co., Ltd. It is a colorful deciduous tree with a height of 6-8 meters. The crown is round or oval, and the new leaves are tender in spring The branches are golden yellow, and turn red after frost in autumn. The fruit hangs all over the branches, and the orange-red arils are exposed after cracking, which is very beautiful. The branches and leaves of golden branches and jade leaves are beautiful and delicate, and their posture is graceful. They can be used as shade trees in gardens and greening at watersides. Jinzhiyu leaves are light-loving, slightly shade-tolerant; cold-resistant, drought-resistant, and water-humidity-resistant; well-developed ...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01H4/00
CPCA01H1/06A01H4/008
Inventor 张丹张茂王东超赵志汝邓坤刘少君
Owner 河南红枫生物高科股份有限公司
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