100 results about "Geneologies" patented technology

The present invention relates to methods of modulating mammalian stem cell and progenitor cell differentiation. The methods of the invention can be employed to regulate and control the differentiation and maturation of mammalian, particularly human stem cells along specific cell and tissue lineages. The methods of the invention relate to the use of certain small organic molecules to modulate the differentiation of stem or progenitor cell populations along specific cell and tissue lineages, and in particular, to the differentiation of embryonic-like stem cells originating from a postpartum placenta or for the differentiation of early progenitor cells to a granulocytic lineage. Finally, the invention relates to the use of such differentiated stem or progenitor cells in transplantation and other medical treatments.

This document provides methods and materials related to genetic variations of developmental disorders. For example, this document provides methods for using such genetic variations to assess susceptibility of developing Autism Spectrum Disorder.

The present disclosure provides an understanding of the regulation of the developmental phases of stem cells and their induction into relevant cell lineages, such as primitive streak, endoderm, mesoderm, or subterrotories of endoderm's. In particular, the present disclosure provides methods, culture medium and kits for the maintenance and differentiation of stem cells into relevant cell lineages.

The compositions and methods described are directed to microRNA chips having a plurality of different oligonucleotides with specificity for genes associated with autism spectrum disorders. The invention further provides methods of identifying microRNA profiles for neurological and psychiatric conditions including autism spectrum disorders, methods of treating such conditions, and methods of identifying therapeutics for the treatment of such neurological and psychiatric conditions.

This invention provides 163 genes that are unique to the L. monocytogenes serovar 4b strain, CLIP80459, compared with L. monocytogenes EGDe strain. These genes are the basis for isolated nucleic acids, hybridization supports, and kits comprising a nucleotide sequence that is unique to a serovar 4b strain of L. monocytogenes. The invention also provides methods for identifying a L. monocytogenes in a sample, methods for identifying the lineage of a L. monocytogenes in a sample, and methods for sub-lineage typing of a L. monocytogenes in a sample. The invention also provides hybridization supports comprising a L. monocytogenes lineage-specific nucleic acid sequence, hybridization supports comprising a L. monocytogenes sub-lineage-specific nucleic acid sequence combination, and kits comprising a nucleic acid or hybridization support of the invention. Additionally, the invention provides specific macroarrays, methods, and kits for use in serotyping L. monocytogenes.

A plasmid, viral or linear DNA molecule containing a nucleic acid sequence derived from the promoter region of the hCol1α2 gene, which is selectively transported into the nuclei of cells in the osteoblast lineage. The sequence can be used independently as a nuclear entry sequence only, and/or as a nuclear entry sequence without regard to position, in a vector or linear DNA that directs gene expression and nuclear entry. The disclosure further includes a chimeric DNA sequence derived by the addition of osteoblast-specific enhancer sequences to the nuclear entry sequence/promoter sequence, to increase osteoblast-specific expression while retaining osteoblast-specific nuclear import. An enhancer sequence is derived from the promoter region of the human Core Binding Factor alpha 1 (Cbfa1/Runx2) gene. The Cbfa1/Runx2 promoter can be added to the sequence derived from, or alternatively, comprising the promoter region of the hCol1α2 gene. Also provided are methods of use of the novel sequences.

The invention discloses a method for performing forest tree multiple-trait pyramiding breeding based on multiple-trait genomic selection. The method sequentially comprises the following steps: clonal individual phenotypic data acquisition; clonal pedigree information and A matrix construction or SNP marker typing data and G matrix construction; multiple-trait model establishment and data analysis. The method disclosed by the invention has the benefits that the workload of forest tree trait phenotype determination can be reduced, and no lamination by the correlation degree between traits and markers exists; compared with a conventional traditional progeny determination technology, multiple-trait orientation and accurate breeding can be realized, an obtained hybrid offspring have reliable productivity and genetic background, the forest tree multiple-trait pyramiding breeding period is significantly shortened, and no specific limitation on tree species and test forest types exists; the forest tree multiple-trait pyramiding breeding purpose can be quickly achieved; therefore, the application prospect is wide.

The present invention belongs to the technical field of cell biology and particularly relates to a technology for regulating and controlling a Jak-Stat pathway to differentiate, dedifferentiate and rejuvenate cells, and an application of the technology. Rejuvenated cell products and/or cell products of different lineage types are obtained by small molecule compound combination, cytokine combination or recombinant protein combination, a gene editing technology and a transgenic technology to quantitatively and/or regularly regulate and control gene or protein targets of the Jak-Stat signaling pathways in cells. The provided technology for regulating and controlling the Jak-Stat signal pathway, and the product/derivatives of the cell product can be used for re-programming cells, tissues, organs and organisms, construct tissue engineering materials, repair damages of tissues and organs of mammals and the aged and degenerated tissues and organs, delay or reverse aging processes of the cells, tissues, organs and organisms, and can be used for immunoregulation of the cells, tissues, organs and organisms.

The present invention relates to use of synthetic factors in reprogramming somatic cells to become induced pluripotent stem cells and other cell lineages. Specifically, the present application relates to fusion proteins containing proteins encoded by cell totipotency-related genes and transcription regulatory domains, their coding sequences, expression vectors, and compositions. The present application also relates to methods for reprogramming somatic cells to become induced pluripotent stem cells and other cell lineages, and cells containing the fusion proteins or the coding sequences.

The invention provides an O-type foot-and-mouth disease recombined phage vaccine of a pig and a construction method thereof. The vaccine adopts recombined T7 phage as an antigen, and the amino acid sequence such as FMDVVP1(129-169) polypeptide of Mya98 pedigree shown in SEQIDNO:2 is expressed on the surface of the recombined T7 phage; and the VP1 (129-169) polypeptide is obtained by inserting single-copy VP1(129-169) genes into the downstream of the P10B gene of the T7 phage to carry out fusion. The invention has the following technical effects that (1) the recombined phage vaccine prepared by the T7 phage carrier can overcome the shortcomings of weaker self immunogenicity of the FMDVVP1 polypeptide and the defect of lack of T auxiliary cell epitope, effectively improve the immunogenicity and stimulate the organism to generate the higher-level antibody; and (2) since the T7 phage is easy to culture, the purification is convenient, and the invention for producing the gene engineering vaccine has multiple advantages. In the O-type foot-and-mouth disease recombined phage vaccine of the pig, the on-scale production is convenient and the cost is low.

This invention relates to DNA microarray technology, and more specifically to methods and kits for identifying autism and autism spectrum disorders in humans.

This disclosure concerns the separation of pericarp tissue from surrounding tissues in grain. Some embodiments concern the isolation of high-purity pericarp DNA from a grain plant that reflects the genotype of the maternal parent of the grain plant, such that the isolated DNA may be used in a PCR-based genotyping assay.

The invention relates to methods and kits for characterizing and diagnosing autism spectrum disorder in an individual based on gene expression levels.

The invention involves inducing a plurality e.g., 3-50 or more mutations (e.g., any whole number between 3 and 50 or more of mutations, with it noted that in some embodiments there can be up to 16 different RNA(s), e.g., sgRNAs each having its own a promoter, in a vector, such an AAV vector or a lentiviral vector and that when each sgRNA does not have its own promoter, there can be twice to thrice that amount of different RNA(s), e.g., sgRNAs, e.g., 32 or even 48 different guides delivered by one vector) in transgenic Cas9 eukaryotes to model a neuronal disease or disorder. The invention comprehends testing putative treatments with such models, e.g., testing putative chemical compounds that may be pharmaceutically relevant for treatment or gene therapy that may be relevant for treatment, or combinations thereof. The invention allows for the study of genetic diseases and putative treatments to better understand and alleviate a genetic disease or a condition, e.g., autism, autism-spectrum disease or disorder, obsessive compulsive disorder, or psychiatric disorders.

The invention relates to a method of detecting the presence of or predisposition to autism, or to an autism spectrum disorder in a subject, the method comprising detecting the combined presence of an alteration in the gene loci of at least PITX1, ATP2B2, EN2, JARID2, MARK1, ITGB3, CNTNAP2, and HOXA1 in a sample from said subject.

This application provides methods to differentiate between subjects with autism spectrum disorder (ASD) and typically developing (TD) or developmentally delayed (DD) subjects using miRNA and/or microbiome levels detected in saliva samples and patient information. The method can be used to monitor the progress of ASD and guide its treatment. RNA-seq, qPCR, or other methods determine counts and abundance of miRNA or microbiomes. MicroRNA and/or microbiome sequencing data are refined by normalization to expression levels or abundance of time-invariant miRNAs and/or microbial RN As to control for time of sample collection or to compensate for circadian fluctuations in these levels. Multivariate logistic regression and nonlinear classification techniques are further used to select a panel of miRNAs and microbiomes that accurately differentiate between subjects with ASD, DD, and TD in subjects with an unknown ASD status. These panels of miRNAs and microbiomes may be developed into a RNA assay kit.

The invention belongs to the field of genetic engineering and relates to an enhancer RNA combination used for diagnosing unexplained recurrent spontaneous abortion, a primer group and an application and a kit thereof. The enhancer RNA combination comprises a first enhancer RNA with more than 90% of homology with a nucleotide sequence shown as SEQ ID NO:1, a second enhancer RNA with more than 90% of homology with a nucleotide sequence shown as SEQ ID NO: 2, and a third enhancer RNA with more than 90% of homology with a nucleotide sequence shown as SEQ ID NO: 3. The screened enhancer RNA combination in the invention serves as a novel marker, the kit used for diagnosing or monitoring the unexplained recurrent spontaneous abortion is prepared, and the kit has the advantages of high sensitivity, wide detection spectrum, low cost and the like.

Human induced pluripotent stem cells (iPSCs) can give rise to multiple cell types and hold great promise in regenerative medicine and disease modeling applications. The Inventors herein developed a reliable two-step protocol to generate human mammary-like organoids from iPSCs. Non-neural ectoderm cell-containing spheres, referred to as mEBs, were first differentiated and enriched from iPSCs using MammoCult medium. Gene expression profile analysis suggested that mammary gland function-associated signaling pathways were hallmarks of 10-d differentiated mEBs. The Inventors generated mammary-like organoids from 10-d mEBs using 3D floating mixed gel culture and a three-stage differentiation procedure. These organoids expressed common breast tissue, luminal, and basal markers, including estrogen receptor, and could be induced to produce milk protein. These results demonstrate that human iPSCs can be directed in vitro toward mammary lineage differentiation.

The invention provides a dual-homologous recombination pedigree tracing technique, and particularly provides a dual-homologous recombination system. In the system, LoxP site and Rox site exist in an embedding manner. By virtue of the system, the other homologous recombination reaction can be prevented by preliminarily selecting one homologous recombination reaction in the existence of Cre recombinant enzyme or Dre recombinant enzyme. By utilizing the dual-homologous recombination system, the genetic recombination operation can be performed for micro organisms, plants and animals.

The invention discloses a screening method and application of a direct mechanical response cell subpopulation. The screened cell subpopulation comprises a Fat <4+> subpopulation, a Fgfr <3 > subpopulation and a Scara <5+> subpopulation, and the invention also discloses application of the mechanical response cell subpopulation in preparation of drugs for local bone defect repair and regeneration and stress-related bone metabolic diseases. Direct mechanical response cells are screened through a single-cell sequencing technology, fine analysis of a cell gene expression profile under stress stimulation is carried out through a single-cell transcriptome level, a key cell subpopulation with fate change under stress regulation is searched for,and in-vitro flow type sorting and in-vitro stress loading are carried out through marker genes. In combination with construction of a CreER-loxP inducible reporter gene system, in-vivo lineage tracing is performed to verify the mechanical responsivenessof the cells, so that the purpose of identifying the direct mechanical response cells is achieved.

The present invention provides a set of specific new molecular detection targets for identifying common serotype listeria monocytogenes. Nucleotide sequences of the specific new molecular detection targets are shown as SEQ ID NO.1-13. The targets can identify different types of the listeria monocytogenes, such as lineage type I, lineage type II, lineage type III, serogroup I.1, serogroup I.2, serogroup II.1, serogroup II.2, serotype 4a and serotype 4c. The present invention also provides a detection method for the common serotype listeria monocytogenes. The method designs specific primers forthe 13 specific target molecules, electrophoresis of PCR amplification products is conducted, and whether detection samples contain the common serotype listeria monocytogenes is directly determined byelectrophoresis results.