10 results about "DNA microarray" patented technology

The invention discloses a DNA microarray material surface preparation method based on polymer and diazonium salt modification, and belongs to the technical field of gene detection.The DNA microarray material surface preparation method comprises the following steps that S1, on the surface of a to-be-modified DNA microarray material, a diazotization reaction is utilized to form a first modification layer, and a first active functional group R1 containing amino is introduced; s2, connecting a second active functional group R2 on the surface of the first modification layer through a covalent reaction to form a second modification layer, and introducing a carbon-carbon double bond; s3, connecting the carbon-carbon double bonds on the surface of the second modification layer with a polymer through a covalent reaction to form a third modification layer, and introducing a third active functional group R3; s4, the surface of the third modification layer is connected with a biomolecular probe through a covalent reaction, a polymer serves as a modification layer on the surface of the chip, amino of an aliphatic chain can be protected in the diazotization process, active sites needed by subsequent experiments can be reserved, and the number of target active sites and the stability of the modification layer are improved.

The invention relates to the technical field of DNA microarrays, and discloses a preparation method and application of a DNA nano array. The preparation method of the DNA nano array comprises the following steps: step 1, acquiring DNA ink, and then transferring the DNA ink to the edge of a substrate material; step 2, moving a flexible platform to enable a probe to be in contact with the DNA ink, then dropping the probe at a first preset position of the substrate material and then lifting the probe to obtain a first DNA nanodot; and 3, changing the target preset position of the substrate material, and repeating the step 2 to obtain the target DNA nanodot. By adopting the method disclosed by the invention, the DNA nano array of which the average diameter is less than or equal to 500 nm, the distance between adjacent points is less than or equal to 2000 nm and the average height is less than or equal to 50 nm can be prepared, the prepared DNA nano array has the characteristics of high density and high flux, meanwhile, the preparation process is simple, the cost is relatively low, and no other toxic chemical reagents are introduced.

Described herein are methods for the synthesis of DNA polynucleotides and polynucleotides, as well as methods for their deprotection and methods for the use of said compounds and compositions comprising said compounds. In particular, such compounds and compositions comprising them are used in methods for light-directed synthesis of DNA microarrays.

To obtain fluorescence images with high detection accuracy. [Solution] The image acquisition device 100 acquires a first fluorescence image of DNA microarray A captured by imaging device 1 during a first period and a second fluorescence image of DNA microarray A captured by imaging device 1 during a second period. Based on the first fluorescence intensity of the fluorescence spots that emit fluorescence in the first fluorescence image, it corrects the second fluorescence intensity of the fluorescence spots that emit fluorescence in the second fluorescence image to generate a corrected second fluorescence image. The corrected second fluorescence image is added to the first fluorescence image to generate an additively corrected fluorescence image of the corrected first fluorescence image.

Technologies include systems, devices, and methods to write, store, read, and perform computation of digital information using nucleic acid molecules (e.g., DNA). The technologies include, for example, a device including one or more individually or block-addressable electrode micro-arrays or nano-arrays to write, store, retrieve, read, and compute / manipulate digital information.

An image acquisition device (200) acquires a fluorescence image obtained by imaging a DNA microarray (20) by an imaging device (2) under a first imaging condition using a predetermined excitation wavelength and a predetermined light receiving wavelength, and acquires a second imaging condition different from the first imaging condition at least one of the excitation wavelength and the light receiving wavelength. A sample noise correction image obtained by imaging a DNA microarray (20) by an imaging device (2) generates a noise-corrected fluorescence image from which sample noise derived from the DNA microarray (20) included in the fluorescence image is removed by using the sample noise correction image.

The invention discloses a method for calculating a synthesis sequence of an in-situ DNA microarray synthesis system, including: obtaining an integer sequence set; constructing a probability database and a probability index database; entering an iterative process, amplifying the sequences in the nodes, updating the matching positions of the amplified nodes, and updating the iterative node set; calculating the guiding values of all nodes in the iterative node set; selecting a dominant node set from the iterative node set, and performing branch pruning according to the dominant node set; clearing the candidate node set, selecting a specified number of candidate nodes from the iterative node set in descending order of the guiding values, and adding them to the candidate node set; randomly selecting a specified number of candidate nodes from the iterative node set and adding them to the candidate node set; checking the amplified integer sequences, and if they are the common super-sequences of the integer sequence set encoded by the sequence set, decoding them according to the encoding rules to obtain a string of characters. It can effectively improve the efficiency of the in-situ DNA microarray synthesis system.

An image acquisition device (100) acquires a first fluorescence image, which is an image obtained by imaging a DNA microarray (A) by an imaging device (1) during a first period, and a second fluorescence image, which is an image obtained by imaging the DNA microarray (A) by the imaging device during a second period. Generating a corrected second fluorescence image in which a second fluorescence intensity of a fluorescent spot in the second fluorescence image is corrected on the basis of a first fluorescence intensity of a fluorescent spot in the first fluorescence image, and superimposing the corrected second fluorescence image on the first fluorescence image; a superimposed corrected fluorescence image obtained by correcting the first fluorescence image is generated.

The application discloses a microfluidic chip for tissue cell nucleus spatial transcriptome sequencing, and belongs to the technical field of particle control in microfluidic chips; the microfluidic chip comprises a free transcriptome capturing layer, a micro-well layer, a code A flow channel layer, a code B flow channel layer and a nucleus transcriptome capturing layer. The micro-well layer is used for placing a tissue slice to be measured, and single cells after dissociation of the tissue slice are captured through the micro-well. The micro-well layer is attached to the free transcriptome capturing layer below, and the free transcriptome capturing layer exposed at the bottom of the micro-well is fixed with mRNA capturing sequences. The code A flow channel layer and the code B flow channel layer are used for fixing cross-type DNA microarrays on the surface of the nucleus transcriptome capturing layer. The code A flow channel layer and the code B flow channel layer are simultaneously used for fixing cross-type DNA microarrays on the free transcriptome capturing layer, so that the common sequencing of single cell spatial free transcriptome and spatial nucleus transcriptome is realized, and the blank of single cell nucleus spatial transcriptome sequencing and the common sequencing of spatial free transcriptome and spatial nucleus transcriptome is filled.

Described herein are methods for the synthesis of DNA polynucleotides and polynucleotides, as well as methods for their deprotection and methods for the use of said compounds and compositions comprising said compounds. In particular, such compounds and compositions comprising them are used in methods for light-directed synthesis of DNA microarrays.