21 results about "Cellular metabolism" patented technology

The invention relates to the technical field of veterinary epidemic disease detection, and discloses a veterinary epidemic disease remote monitoring and intelligent diagnosis system, which comprises a multi-mode biosensor array including an implantable miniature biochip, a wearable flexible physiological monitoring patch and an environment sensing sensor cluster, the implantable miniature biochip is used for being implanted into a key tissue organ area in an animal body and collecting microscopic physiological data such as cellular metabolism signals and local immune reaction marker concentration in real time, and the wearable flexible physiological monitoring patch is used for being attached to the body surface of the animal. By means of the multi-mode biosensor array and the edge intelligent preprocessing unit, animal physiology and environment data are accurately monitored in real time. The multi-modal biosensor array covers the implantable miniature biochip, the wearable flexible physiological monitoring patch and the environment sensing sensor cluster, and can comprehensively collect in-vivo microscopic physiological data, body surface macroscopic physiological parameters and feeding environment information of animals.

A class of compounds that bind to fatty acid binding protein 4 (FABP4) and regulate adipocyte metabolism to promote enhanced glucose utilization, as well as pharmaceutical compositions comprising the class of compounds in combination with a pharmaceutically acceptable diluent or carrier, optionally further in combination with a therapeutically active agent, and the use of these compounds in the preparation of medicines and medicaments in the treatment of disorders affecting FABP4. JPEG2023518648000225.jpg89103

The invention relates to application of engineered clostridium butyricum for producing tryptophan in delaying senescence and improving metabolism, and belongs to the technical field of biomedical engineering. The invention discloses an application of engineered clostridium butyricum for expressing tryptophan biosynthetase trpEDCBA in preparation of drugs for delaying senescence, improving metabolism and increasing intestinal beneficial flora. An aging mouse model is constructed, it is found that after the aged mouse orally takes the engineered clostridium butyricum, the muscle strength, the blood glucose homeostasis, the cell metabolism, the cognitive ability and other aspects are remarkably improved, the improvement effect of the engineered clostridium butyricum is better than that of direct taking of butyric acid and tryptophan, the tryptophan-producing clostridium butyricum can be colonized in intestinal tracts for a long time, and the tryptophan-producing effect of the engineered clostridium butyricum is improved. The abundance of effective microbial communities in intestinal tracts is improved, a new tool and means are provided for anti-aging, and good application prospects are achieved.

According to the self-energized biological 3D printing bio-ink, the 3D printing bioactive scaffold and the preparation method of the self-energized biological 3D printing bio-ink, the controllable-release self-energized biological 3D printing bio-ink is prepared by mixing the organic high-molecular polymers with different molecular weights and the energy metabolism promoting substances, and the ink is good in biocompatibility; by changing the content of energy supply substances, the ink can be designed to'provide 'and'accept' different cell metabolism regulation clues in an adjustable time regulation manner, better migration and adhesion of cells are realized, and functions are exerted, and meanwhile, the ink has controllable slow release of energy metabolism promoting substances and generates micro-nano pores when being used for constructing a three-dimensional bracket through 3D printing, so that the energy metabolism promoting effect is improved. The hydrophilicity of the scaffold is improved, the biological energy metabolism activity of the composite material is enhanced, the energy metabolism promoting substance can provide a sufficient nutrition environment for cell growth, the cell energy metabolism process is regulated and controlled, and adhesion, growth, proliferation and functionalization of cells are facilitated.

The present application provides a kind of composite hydrogel for scar repair and its preparation method, including photo-crosslinking hyaluronic acid methacrylate and layered silicate nanocrosslinking agent dispersed in photo-crosslinking hyaluronic acid methacrylate, wherein photo-crosslinking hyaluronic acid methacrylate is physically adsorbed with layered silicate nanocrosslinking agent to form a double crosslinking network structure.The present application can be firmly attached to the wound to prevent bacterial invasion, and can also release the long-acting, controllable slow release of load factor, extend the local treatment window, improve bioavailability, and through the double crosslinking structure, it can also simulate extracellular matrix, facilitate the transport of oxygen, nutrients and cell metabolic waste, greatly improve the scar repair effect.

An exemplary apparatus and method are disclosed that can provide a fully instrumented microphysiological system having a microchamber for the culture and monitoring of cells and biological samples. The exemplary apparatus is supported by integrated bioelectronic circuits and a graphical user interface for easy user configuration and monitoring. The system has integrated microfluidics to maintain its microphysiological environment within each well. The exemplary apparatus incorporates metabolic and environment sensors inside each well, allowing multiple wells to perform concurrent measurements in real-time.

The invention provides a cell three-dimensional dynamic culture micro-fluidic chip which is suitable for cell three-dimensional culture, drug screening, tumor migration research and the like. The cell culture micro-device is composed of three layers including a cell sample injection layer, a cell scaffold layer and a culture solution sample injection layer. The device is characterized in that the cell sampling layer has a concave-convex structure; the culture solution sample injection layer simulates a human vascular structure and is provided with a permeable wall. Wherein the 3D printing cell growth bracket is embedded in a unit chamber of the culture solution sample injection layer and is assembled by a convex structure on the cell sample injection layer in an elastic interference fit manner, so that the bracket is prevented from being scoured and deformed by liquid. The invention has the advantages that: 1, the cells are uniformly injected and distributed in the cell scaffold; 2, a three-dimensional growth space is provided for cells; 3, nutrition is supplied to the cells, and cell metabolism waste liquid is discharged in time; 4, the bracket is stable in structure and can resist liquid scouring deformation; and 5, the micro-fluidic device can become a functional unit of an integrated micro-fluidic device and has wide applicability.

The invention discloses a method for enhancing the synthesis capacity of a yeast double-metabolic-pathway pigment product and application, and belongs to the field of synthetic biology and metabolic engineering. The method comprises the following steps: constructing a saccharomyces cerevisiae engineering strain capable of simultaneously synthesizing violacein and lycopene; constructing a mutant library of the global transcription factor SPT15; transforming the mutant library into the engineering strain, and screening the mutant strain with increased yield based on bacterial colony color visualization. According to the invention, a single global transcription factor SPT15 is modified, so that the cell metabolism is regulated and controlled on the whole, the synergistic interaction of a diheterologous synthesis pathway is realized, and the tedious multi-gene rational modification is avoided. The method is easy and convenient to operate and short in period, and a new strategy is provided for efficient construction of yeast cell factories for high-yield pigment and other double-way products.

A single-cell metabolomics analysis apparatus and method are provided. The apparatus includes: a sample introduction module for introducing a cell suspension to be analyzed into the apparatus; a cell separation module for receiving the cell suspension from the sample introduction module and aligning the cells in the cell suspension flowing through it linearly and spaced apart from each other; a transfer line for receiving the cell suspension from the cell separation module and allowing the cell suspension to flow through it; a fluorescence detection module disposed in the upstream section of the transfer line for detecting the fluorescence signal of each individual cell in the cell suspension; an ionization module disposed in the downstream section of the transfer line for ionizing the metabolites of each individual cell; and a mass spectrometry detection module disposed downstream of the transfer line for detecting the metabolomic signal of each individual cell.

The invention relates to the technical field of animal experiment models, and discloses a feed composition capable of prolonging the life of mice and delaying senescence and application, and the feed composition is prepared by the following steps: raw material pretreatment: corn flour, soybean meal and wheat bran are respectively crushed and sieved by a 80-100 mesh sieve, and fish meal is subjected to ultraviolet sterilization treatment for 10-20 min; additive premixing: metformin and a carrier are mixed and ground, the carrier is microcrystalline cellulose or corn starch, and the mass ratio of metformin to the carrier is (1: 50)-(1: 100); and gradient mixing. Metformin in the feed composition activates an AMPK signal channel, enhances the cell energy perception ability, promotes a metabolic mode to be converted to mitochondrial oxidative phosphorylation direction and reduces glycolysis dependence, meanwhile, NAD + precursor substances in the synergistic components promote biosynthesis of nicotinamide adenine dinucleotide and drive the activity of SIRT deacetylase to be continuously improved, and the effect of improving the activity of the SIRT deacetylase is achieved. The cell metabolism homeostasis is jointly reconstructed, and the decline of senescence-related pathways is delayed from the molecular level.

The invention discloses a co-culture bottle for different types of cells, which comprises a bottle body, a separation membrane is arranged in the bottle body, the interior of the bottle body is divided into a first culture area and a second culture area by the separation membrane, the bottle body is provided with a first bottle opening and a second bottle opening, the first bottle opening is communicated with the first culture area, and the second bottle opening is communicated with the second culture area. And the second bottle opening is communicated with the second culture area. The bottle body is divided into the first culture area and the second culture area which are independent from each other through the separation membrane, so that physical isolation of different types of cells can be realized, target cell purity reduction caused by mutual confusion of the cells is avoided, free exchange of small molecular substances such as a culture medium, cell metabolites and growth factors can be allowed by utilizing a microporous structure, and the cell culture efficiency is improved. The requirement of interaction between cells through substance signals in co-culture is met, and the in-vivo cell microenvironment is simulated to the maximum extent.

The present disclosure relates to a liver support system comprising (a) a single mining column; and (b) a detoxification column comprising a plurality of liver tissues, wherein the liver support system is configured to allow fluid to flow from the apheresis column to the detoxification column. The detoxification column can provide detoxification, secretion and regeneration functions. The detoxification may include promotion of severely impaired central urea cycle as well as CYP function that is crucial for detoxification of exogenous materials, cellular metabolism, and homeostasis. The single sampling column can play a role in adsorbing granulocytes from fluid and protecting a plurality of liver tissues from inflammatory injury. The present disclosure relates to methods of using a liver support system and engineered cells for use in a liver support system.

An RNA-binding protein (RBP) AbrP of SEQ ID NO:1 is claimed. The AbrP may bind to translation-promoting factors FusA, LepA, and Era separately, or alternatively the AbrP binds to a characteristic sequence of an sRNA. A complex resulting from the combination of the AbrP and the promoting factor can form a translation system of a target mRNA under a guidance of the sRNA, thus releasing the translation-promoting factors FusA, LepA, and Era to stimulate and promote the RNA translation into the encoded protein. Said system has practical application for a regulatory element or regulatory system in regulating cell metabolic engineering or synthetic biology.

The invention belongs to the technical field of organoid, and particularly relates to a core-shell structure vascularized bone organoid and a preparation method and application thereof. The MSCs and the ECs are cultured by adopting an organoid culture mode, the cell spheroid with a'core-shell 'structure is constructed by using the MSCs / ECs with a specific cell proportion, a microenvironment for in-vivo growth of cells can be better simulated, cell-cell connection and a complete extracellular matrix are preserved, the cell spheroid has a structure and a function similar to those of source cells or directionally differentiated stem cells, and the cell spheroid has a good application prospect. And the development process of organs is simulated, so that higher self-renewal and proliferation capabilities are achieved, the metabolic capability of cells is improved, the cell viability is improved, and good proliferation, osteogenesis and vascularization effects are achieved. The vascularized bone organ prepared by the method can be widely applied to preparation of bone repair materials.

The invention relates to the technical field of bioengineering, in particular to a xanthan gum engineering strain construction method based on secondary messenger and metabolic engineering, and the method comprises the following steps: illuminating a molecular mechanism of c-di-GMP and ppGPP for cooperatively regulating xanthan gum synthesis; a high-yield strain is constructed through secondary messenger module optimization and metabolic engineering modification; and fermentation parameters are optimized by combining a precise fermentation technology. By analyzing a secondary messenger regulation mechanism and optimizing a chassis cell metabolism network, the production performance of xanthan gum is remarkably improved. According to the invention, a c-di-GMP and ppGPP synergistic regulation mechanism is clarified, and secondary messenger module optimization, metabolic engineering transformation and precise fermentation are combined, so that the xanthan gum high-yield strain is constructed, and the yield and sugar conversion rate are improved.

The invention discloses a cRGDyk-mitochondria composite delivery system of targeted hepatic stellate cells as well as a preparation method and application of the cRGDyk-mitochondria composite delivery system. According to the compound system, cRGDyk targeting peptide and exogenous mitochondria are used as main functional units, covalent linkage is performed through a thiol-maleimide click chemical reaction, and cerium oxide nanoparticles with mimic enzyme activity are introduced. According to the system, efficient targeting of activated hepatic stellate cells is achieved through cRGDyk peptide, exogenous mitochondria is used for repairing the cell metabolism function, cerium oxide nanoparticles provide auxiliary ROS removal capacity, the three components have a synergistic effect, the obvious hepatic fibrosis reversing effect is shown in an in-vitro cell model and an in-vivo animal model, biocompatibility is good, and the system is suitable for clinical application. Wide clinical application prospects are realized.

A method for predicting the metabolic state of a cell culture of cells of a specific cell type includes providing a metabolic model of a cell of the specific cell type, and performing at each of a plurality of points in time during cultivation of the cell culture, receiving measured concentrations of a plurality of extracellular metabolites and a measured cell density in the culture medium; inputting the received measurements as input parameter values to a trained machine learning program logic-MLP; predicting extracellular fluxes of the extracellular metabolites at a future point in time by the MLP; performing metabolic flux analysis to calculate the intracellular fluxes at the future point in time based on the predicted extracellular fluxes and the stoichiometric equations of the metabolic model.

A system and method for predictive modeling of cellular systems assesses compound effectiveness on cellular metabolic and inflammatory states. Cellular samples are exposed to metabolic MRX sensors detecting oxidative stress, reporting cytoskeletal organization, or monitoring mitochondrial morphology, dynamics, or compartment-specific redox activity. Images of MRX sensor-labeled samples are captured using an imaging device and analyzed by trained predictive models to determine compound impact on immunological activation, inflammatory status, oxidative stress, mitochondrial function, and bio-energetic state. The system supports multisensor integration and spectral unmixing to generate multidimensional metabolic or ROS related MRX fingerprints that characterize cellular phenotypes, drug responses, and enable comparison to reference states. Preprocessing modules perform image normalization, augmentation, segmentation, and feature extraction to enhance model performance. Predictive models classify treatment conditions, identify perturbation-specific signatures, and enable compound ranking or mechanism-of-action inference. The system provides an objective, scalable, and high-content platform for drug screening, toxicity assessment, and inflammation research.

The invention relates to the technical field of cell and 3D living body tissue culture, in particular to an organ chip which comprises a primary cell and 3D living body tissue culture unit, a connecting unit and a secondary cell and 3D living body tissue culture unit which are connected in sequence, cell metabolites in the primary cell and 3D living tissue culture unit flow into the secondary cell and 3D living tissue culture unit through the connecting unit; the secondary cell and 3D living body tissue culture unit comprises at least one secondary cell and 3D living body tissue culture chamber; and a confocal observation slide is arranged at the bottom of the secondary cell and 3D living tissue culture room. According to the invention, the structural form that the primary cell and 3D living body tissue culture unit is connected with the secondary cell and 3D living body tissue culture unit through the connecting unit is designed, and the secondary cell and 3D living body tissue culture unit is used as a real-time dynamic monitoring unit to dynamically monitor cells and 3D living body tissue secretions in real time.

The invention relates to the technical field of engineering cells, in particular to a bimodal engineering cell based on cell transfection and intracellular gelling and a construction method of the bimodal engineering cell. Target cell membrane protein is overexpressed on the cell surface of the bimodal engineering cell, and gelation is carried out in the cell. The preparation process comprises the following steps: preparing a cell transfected with a target cell membrane protein gene; and resuspending the cell transfected with the target cell membrane protein gene in a hydrogel solution, and carrying out freeze thawing treatment and photocuring treatment to obtain the bimodal engineering cell. According to the technical scheme, the technical problem that the consistency of the content, the type and the space structure of the membrane protein and source cells is difficult to accurately maintain while the metabolic activity of the cells is deprived in the prior art can be solved. According to the scheme, a cell transfection and intracellular gelation synergistic strategy is utilized, and the product can be used for drug delivery or selective biological electron affinity sensing, can adapt to different types of target membrane proteins, and is compatible with the requirements of multiple fields such as cell therapy, disease biomarker detection and targeted drug delivery.

The invention discloses a feed supplement additive suitable for HEK293 cell culture and application, and the feed supplement additive comprises the following raw material substances: 50-90 g / L of amino acid, 1-3 g / L of vitamin, 3-5.5 g / L of inorganic salt, 5.5-25 mg / L of trace element, 1-3 mg / L of fatty acid, 220-460 mg / L of lipid, 100-260 mg / L of polyamine, and 40-60 g / L of glucose. According to the HEK293 cell metabonomics and nutrition consumption conditions, the additive suitable for material supplementation in the HEK293 cell culture process is obtained under the condition that protein is not used, the additive is excellent in culture effect, beneficial to improving the antibody expression quantity and capable of avoiding instability caused by degradation of protein, and the additive is good in stability, high in yield and good in stability. And the cost is low, the universality is wide, and the use is convenient.