12 results about "Cellular metabolism" patented technology

A class of compounds that bind to fatty acid binding protein 4 (FABP4) and regulate adipocyte metabolism to promote enhanced glucose utilization, as well as pharmaceutical compositions comprising the class of compounds in combination with a pharmaceutically acceptable diluent or carrier, optionally further in combination with a therapeutically active agent, and the use of these compounds in the preparation of medicines and medicaments in the treatment of disorders affecting FABP4. JPEG2023518648000225.jpg89103

The present application provides a kind of composite hydrogel for scar repair and its preparation method, including photo-crosslinking hyaluronic acid methacrylate and layered silicate nanocrosslinking agent dispersed in photo-crosslinking hyaluronic acid methacrylate, wherein photo-crosslinking hyaluronic acid methacrylate is physically adsorbed with layered silicate nanocrosslinking agent to form a double crosslinking network structure.The present application can be firmly attached to the wound to prevent bacterial invasion, and can also release the long-acting, controllable slow release of load factor, extend the local treatment window, improve bioavailability, and through the double crosslinking structure, it can also simulate extracellular matrix, facilitate the transport of oxygen, nutrients and cell metabolic waste, greatly improve the scar repair effect.

An exemplary apparatus and method are disclosed that can provide a fully instrumented microphysiological system having a microchamber for the culture and monitoring of cells and biological samples. The exemplary apparatus is supported by integrated bioelectronic circuits and a graphical user interface for easy user configuration and monitoring. The system has integrated microfluidics to maintain its microphysiological environment within each well. The exemplary apparatus incorporates metabolic and environment sensors inside each well, allowing multiple wells to perform concurrent measurements in real-time.

The invention discloses a method for enhancing the synthesis capacity of a yeast double-metabolic-pathway pigment product and application, and belongs to the field of synthetic biology and metabolic engineering. The method comprises the following steps: constructing a saccharomyces cerevisiae engineering strain capable of simultaneously synthesizing violacein and lycopene; constructing a mutant library of the global transcription factor SPT15; transforming the mutant library into the engineering strain, and screening the mutant strain with increased yield based on bacterial colony color visualization. According to the invention, a single global transcription factor SPT15 is modified, so that the cell metabolism is regulated and controlled on the whole, the synergistic interaction of a diheterologous synthesis pathway is realized, and the tedious multi-gene rational modification is avoided. The method is easy and convenient to operate and short in period, and a new strategy is provided for efficient construction of yeast cell factories for high-yield pigment and other double-way products.

A single-cell metabolomics analysis apparatus and method are provided. The apparatus includes: a sample introduction module for introducing a cell suspension to be analyzed into the apparatus; a cell separation module for receiving the cell suspension from the sample introduction module and aligning the cells in the cell suspension flowing through it linearly and spaced apart from each other; a transfer line for receiving the cell suspension from the cell separation module and allowing the cell suspension to flow through it; a fluorescence detection module disposed in the upstream section of the transfer line for detecting the fluorescence signal of each individual cell in the cell suspension; an ionization module disposed in the downstream section of the transfer line for ionizing the metabolites of each individual cell; and a mass spectrometry detection module disposed downstream of the transfer line for detecting the metabolomic signal of each individual cell.

The present disclosure relates to a liver support system comprising (a) a single mining column; and (b) a detoxification column comprising a plurality of liver tissues, wherein the liver support system is configured to allow fluid to flow from the apheresis column to the detoxification column. The detoxification column can provide detoxification, secretion and regeneration functions. The detoxification may include promotion of severely impaired central urea cycle as well as CYP function that is crucial for detoxification of exogenous materials, cellular metabolism, and homeostasis. The single sampling column can play a role in adsorbing granulocytes from fluid and protecting a plurality of liver tissues from inflammatory injury. The present disclosure relates to methods of using a liver support system and engineered cells for use in a liver support system.

The invention belongs to the technical field of organoid, and particularly relates to a core-shell structure vascularized bone organoid and a preparation method and application thereof. The MSCs and the ECs are cultured by adopting an organoid culture mode, the cell spheroid with a'core-shell 'structure is constructed by using the MSCs / ECs with a specific cell proportion, a microenvironment for in-vivo growth of cells can be better simulated, cell-cell connection and a complete extracellular matrix are preserved, the cell spheroid has a structure and a function similar to those of source cells or directionally differentiated stem cells, and the cell spheroid has a good application prospect. And the development process of organs is simulated, so that higher self-renewal and proliferation capabilities are achieved, the metabolic capability of cells is improved, the cell viability is improved, and good proliferation, osteogenesis and vascularization effects are achieved. The vascularized bone organ prepared by the method can be widely applied to preparation of bone repair materials.

The invention relates to the technical field of bioengineering, in particular to a xanthan gum engineering strain construction method based on secondary messenger and metabolic engineering, and the method comprises the following steps: illuminating a molecular mechanism of c-di-GMP and ppGPP for cooperatively regulating xanthan gum synthesis; a high-yield strain is constructed through secondary messenger module optimization and metabolic engineering modification; and fermentation parameters are optimized by combining a precise fermentation technology. By analyzing a secondary messenger regulation mechanism and optimizing a chassis cell metabolism network, the production performance of xanthan gum is remarkably improved. According to the invention, a c-di-GMP and ppGPP synergistic regulation mechanism is clarified, and secondary messenger module optimization, metabolic engineering transformation and precise fermentation are combined, so that the xanthan gum high-yield strain is constructed, and the yield and sugar conversion rate are improved.

The invention discloses a cRGDyk-mitochondria composite delivery system of targeted hepatic stellate cells as well as a preparation method and application of the cRGDyk-mitochondria composite delivery system. According to the compound system, cRGDyk targeting peptide and exogenous mitochondria are used as main functional units, covalent linkage is performed through a thiol-maleimide click chemical reaction, and cerium oxide nanoparticles with mimic enzyme activity are introduced. According to the system, efficient targeting of activated hepatic stellate cells is achieved through cRGDyk peptide, exogenous mitochondria is used for repairing the cell metabolism function, cerium oxide nanoparticles provide auxiliary ROS removal capacity, the three components have a synergistic effect, the obvious hepatic fibrosis reversing effect is shown in an in-vitro cell model and an in-vivo animal model, biocompatibility is good, and the system is suitable for clinical application. Wide clinical application prospects are realized.

A method for predicting the metabolic state of a cell culture of cells of a specific cell type includes providing a metabolic model of a cell of the specific cell type, and performing at each of a plurality of points in time during cultivation of the cell culture, receiving measured concentrations of a plurality of extracellular metabolites and a measured cell density in the culture medium; inputting the received measurements as input parameter values to a trained machine learning program logic-MLP; predicting extracellular fluxes of the extracellular metabolites at a future point in time by the MLP; performing metabolic flux analysis to calculate the intracellular fluxes at the future point in time based on the predicted extracellular fluxes and the stoichiometric equations of the metabolic model.

A system and method for predictive modeling of cellular systems assesses compound effectiveness on cellular metabolic and inflammatory states. Cellular samples are exposed to metabolic MRX sensors detecting oxidative stress, reporting cytoskeletal organization, or monitoring mitochondrial morphology, dynamics, or compartment-specific redox activity. Images of MRX sensor-labeled samples are captured using an imaging device and analyzed by trained predictive models to determine compound impact on immunological activation, inflammatory status, oxidative stress, mitochondrial function, and bio-energetic state. The system supports multisensor integration and spectral unmixing to generate multidimensional metabolic or ROS related MRX fingerprints that characterize cellular phenotypes, drug responses, and enable comparison to reference states. Preprocessing modules perform image normalization, augmentation, segmentation, and feature extraction to enhance model performance. Predictive models classify treatment conditions, identify perturbation-specific signatures, and enable compound ranking or mechanism-of-action inference. The system provides an objective, scalable, and high-content platform for drug screening, toxicity assessment, and inflammation research.

The invention relates to the technical field of engineering cells, in particular to a bimodal engineering cell based on cell transfection and intracellular gelling and a construction method of the bimodal engineering cell. Target cell membrane protein is overexpressed on the cell surface of the bimodal engineering cell, and gelation is carried out in the cell. The preparation process comprises the following steps: preparing a cell transfected with a target cell membrane protein gene; and resuspending the cell transfected with the target cell membrane protein gene in a hydrogel solution, and carrying out freeze thawing treatment and photocuring treatment to obtain the bimodal engineering cell. According to the technical scheme, the technical problem that the consistency of the content, the type and the space structure of the membrane protein and source cells is difficult to accurately maintain while the metabolic activity of the cells is deprived in the prior art can be solved. According to the scheme, a cell transfection and intracellular gelation synergistic strategy is utilized, and the product can be used for drug delivery or selective biological electron affinity sensing, can adapt to different types of target membrane proteins, and is compatible with the requirements of multiple fields such as cell therapy, disease biomarker detection and targeted drug delivery.