17 results about "Bioactive scaffold" patented technology

According to the self-energized biological 3D printing bio-ink, the 3D printing bioactive scaffold and the preparation method of the self-energized biological 3D printing bio-ink, the controllable-release self-energized biological 3D printing bio-ink is prepared by mixing the organic high-molecular polymers with different molecular weights and the energy metabolism promoting substances, and the ink is good in biocompatibility; by changing the content of energy supply substances, the ink can be designed to'provide 'and'accept' different cell metabolism regulation clues in an adjustable time regulation manner, better migration and adhesion of cells are realized, and functions are exerted, and meanwhile, the ink has controllable slow release of energy metabolism promoting substances and generates micro-nano pores when being used for constructing a three-dimensional bracket through 3D printing, so that the energy metabolism promoting effect is improved. The hydrophilicity of the scaffold is improved, the biological energy metabolism activity of the composite material is enhanced, the energy metabolism promoting substance can provide a sufficient nutrition environment for cell growth, the cell energy metabolism process is regulated and controlled, and adhesion, growth, proliferation and functionalization of cells are facilitated.

The present invention discloses a bioactive scaffold for slowly releasing Apt19S to recruit endogenous stem cells and guide the straight regeneration of central nervous system axons and its application. The bioactive scaffold is composed of acellular optic nerve (DON) and aptamer Apt19S, and its preparation method comprises the following steps: 1. preparing a DON scaffold. 2. activating the carboxyl group on the surface of the DON scaffold. 3. The DON scaffold is combined with amino-modified Apt19S through an amidation reaction to obtain the DON‑Apt19S scaffold (abbreviated as DON‑A). This bioactive scaffold can continuously release Apt19S, recruit ALPL-positive endogenous neural stem cells and mesenchymal cells, guide the straight regeneration of central nervous system axons, and provide a microenvironment for neurogenesis and angiogenesis for in situ spinal cord repair. The bioactive scaffold has important transformation prospects and application value in the repair of central nervous system injuries.

The invention relates to a biological material loaded active bracket as well as a preparation method and application thereof. The human extracellular vesicles in the biological material-loaded active scaffold can regulate the functions of immune cells, inhibit the generation of inflammatory factors and relieve inflammatory response; the human extracellular matrix is rich in various collagens (such as type I collagen and type III collagen), elastin, fibronectin, sodium hyaluronate and other substances, has multi-dimensional advantages in the aspect of treating psoriasis, has the characteristic of being adaptive to the pathological mechanism of psoriasis, and can improve diseases from multiple levels; the human extracellular matrix and the hydrogel material can provide a good growth microenvironment for skin cells, promote normal proliferation and differentiation of keratinocytes, and recover normal structure and function of skin; the human extracellular vesicles and the human extracellular matrix have a synergistic effect, and psoriasis can be effectively treated.

An implantable tissue scaffold is provided comprising a mixture of a biocompatible organic polymer and chitin wherein the chitin is embedded in the biocompatible organic polymer. Also provided is a composition for 3D printing comprising at least one biocompatible organic polymer and chitin that can be partially deacetylated wherein the chitin is embedded within the biocompatible organic polymer. Further provided is a method of promoting tissue formation, the method comprising implanting a tissue scaffold comprising a mixture of a biocompatible organic polymer and chitin at a site where regeneration bone tissue formation is required.

Disclosed herein is a versatile injectable nano-bioactive scaffold designed to achieve both immediate soft tissue volumization and long-term tissue regeneration. By utilizing biocompatible polymers such as hyaluronic acid, chitosan, and alginate, along with self-assembling peptides and nano-structured carriers including mesoporous silica nanoparticles and liposomes, the system facilitates controlled release of essential growth factors. This innovative approach delivers sustained bioactivity, significantly reducing the need for repeated injections and minimizing inflammatory complications. The system promises superior clinical outcomes, advancing the field of tissue engineering and regenerative medicine by providing an effective and lasting solution for tissue repair and regeneration.

Provided is an implantable tissue scaffold comprising a mixture of a biocompatible organic polymer and chitin, wherein the chitin is embedded in the biocompatible organic polymer. Also provided is a composition for 3D printing, the composition comprising at least one biocompatible organic polymer and chitin that may be partially deacetylated, wherein the chitin is embedded within the biocompatible organic polymer. Further provided is a method of promoting tissue formation, comprising implanting a tissue scaffold comprising a mixture of a biocompatible organic polymer and chitin at a site in need of regenerative bone tissue formation.

The invention discloses a preparation method of a bioactive scaffold for promoting nerve vascularization bone regeneration. The preparation method comprises the following steps: mixing a synthetic high polymer material and / or a natural high polymer material with an osteogenesis promoting nano material to obtain printing ink; printing ink is used in a 3D printing mode, and the basic support is obtained. The preparation method comprises the following steps: preparing a piezoelectric high polymer material into a micro-nano fiber membrane through an electrostatic spinning technology, cutting the micro-nano fiber membrane into small blocks, soaking the small blocks in an ethanol solution, and dispersing and filtering the small blocks to obtain a micro-nano fiber / ethanol dispersion liquid; and immersing the basic scaffold in the micro-nano fiber / ethanol dispersion liquid for micro-nano fiber self-assembly, then removing ethanol, placing and drying to obtain the bioactive scaffold. The scaffold has a micro-nano fiber structure of a bionic natural extracellular matrix, and cell behaviors of adhesion, proliferation, differentiation and the like of cells are promoted. Cells in contact with the stent can exert a self-stimulation bioelectric effect driven by cell adhesion, so that efficient nerve vascularization bone regeneration can be realized, and the quality of bone defect repair is improved.

To provide a biocompatible membrane composite capable of providing an environment capable of mitigating or controlling a foreign body reaction.SOLUTION: The membrane composite includes a conformable layer and a vascularizing layer. A reinforcing component can optionally be included to provide support to the biocompatible membrane composite invivo and prevent distortion of the membrane composite. The relief layer may be coupled or attached to the implantable device and / or the cell system. The biocompatible membrane composite can be used as a surface layer for implantable devices or cell systems that require vascularization for function but require protection from a host immune response, such as the formation of foreign body giant cells. The biocompatible membrane composite can partially or completely cover the exterior of the implantable device or cell system. The relief layer is positioned between the implantable device or bioactive scaffold and the vascularizing layer.SELECTED DRAWING: Figure 7

The invention relates to a three-dimensional bioactive scaffold with ordered micro-nanowires modified on the surface as well as a preparation method and application of the three-dimensional bioactive scaffold, and belongs to the field of biological materials. The three-dimensional bioactive scaffold comprises a bioactive scaffold base body and a topological structure layer modified on the surface of the bioactive scaffold base body, and the topological structure layer is composed of micro-nanowires which are orderly and directionally arranged in the fixed direction. The ordered micro-nano topological structure is successfully integrated into the three-dimensional tissue engineering scaffold, effective physical intervention can be performed on various cells, and meanwhile, the three-dimensional tissue engineering scaffold can be used as a flexible tissue engineering platform for regeneration of complex tissues. Besides, the 3D printing technology and the surface brushing technology are combined, the bioactive scaffold with the ordered micro-nanowires on the surface is constructed, and the method has important significance on development of tissue engineering scaffolds.

This invention belongs to the field of biomaterials technology, specifically relating to a bioactive scaffold, its preparation method, and its applications. This invention incorporates functionalized mesoporous silica nanoparticles loaded with aminoguanidine into the skin secretions of the giant salamander and polycaprolactone electrospun fibers to construct a bioactive composite scaffold (SPMA). This scaffold enables the sustained release of AG and osteogenic factors, effectively inhibiting free radical generation, AGEs toxicity, and inflammatory responses. Furthermore, it significantly improves the survival rate, osteogenic differentiation, and mineralization capacity of bone marrow mesenchymal stem cells in a high-glucose microenvironment. In vivo experiments further demonstrate that in a streptozotocin-induced diabetic mouse model of skull defects, the SPMA scaffold can alleviate local inflammation, promote angiogenesis, and significantly accelerate new bone formation and defect repair.

The invention relates to a multilevel-structure bioactive three-dimensional scaffold for multi-cell interaction and neurotized bone regeneration as well as a preparation method and application of the multilevel-structure bioactive three-dimensional scaffold. The multi-stage structure bioactive three-dimensional scaffold comprises a bioactive scaffold base body with a macroscopic channel structure and a microcosmic directional topological structure filled in the base body, the macroscopic channel structure has a three-dimensional controllable diversified structure, and the microcosmic directional topological structure is a directional micron-sized channel filled in the microcosmic directional topological structure; the multi-level structure bioactive three-dimensional scaffold provided by the invention can be used as a'space bridge 'for coordinating space-time interaction of multiple cells, and has a beneficial promotion effect on mutual migration, energy metabolism level and specific differentiation behavior of multiple cells in a three-dimensional space; the multi-stage structure bioactive three-dimensional scaffold constructed by the invention is a novel bone repair biological material with a multi-stage structure and biological activity, and a new strategy is provided for large-section bone repair and other complex tissue reconstruction.

The invention relates to bio-ink, a 4D bioactive scaffold and a preparation method and application of the 4D bioactive scaffold, and belongs to the technical field of biology. The biological ink provided by the invention is prepared from recombinant mussel mucin, an epidermal growth factor (EGF), methacrylic anhydride gelatin, hyaluronic acid, polydopamine, N-isopropylacrylamide, trehalose, tetrabutylammonium bromide, LAP and 18-crown ether-6. The bio-ink is used for preparing a 4D bioactive scaffold, and the obtained scaffold has a good cell adhesion effect, can release active factors as required, has temperature responsiveness, can trigger deformation at the body temperature of 37 DEG C, realizes rapid repair of tissues, is good in safety, and can be applied to tissue regeneration and medical beauty repair.

This invention relates to a three-dimensional porous bioactive scaffold, a vascularized bone organoid, its preparation method, and its applications, belonging to the field of tissue engineering technology. The bioactive scaffold includes a scaffold body, a Piezo1 channel agonist loaded on the scaffold body, and an Nrf2 agonist and / or Nrf2 expression vector loaded on the scaffold body; the scaffold body is a 3D-printed PMBG / β-TCP composite porous scaffold. The vascularized bone organoid uses the three-dimensional porous bioactive scaffold as a supporting framework and also includes cellular components, including co-cultured bone marrow mesenchymal stem cells and endothelial cells. Compared with existing technologies, this invention successfully constructs a novel vascularized bone organoid capable of targeting the Piezo1-Nrf2 signaling axis and possessing anti-endothelial aging and mechanical enhancement functions.

The present application relates to a conch-like bioactive scaffold and a preparation method and application thereof. The conch-like bioactive scaffold has a spiral structure feature; the whole scaffold comprises a middle support, a peripheral wall containing a through-hole structure, and a spiral-shaped node and a spiral-shaped cavity integrally formed between the middle support and the peripheral wall.

This invention relates to a three-dimensional porous bioactive scaffold, a vascularized bone organoid, its preparation method, and its applications, belonging to the field of tissue engineering technology. The bioactive scaffold includes a scaffold body, a Piezo1 channel agonist loaded on the scaffold body, and an Nrf2 agonist and / or Nrf2 expression vector loaded on the scaffold body; the scaffold body is a 3D-printed PMBG / β-TCP composite porous scaffold. The vascularized bone organoid uses the three-dimensional porous bioactive scaffold as a supporting framework and also includes cellular components, including co-cultured bone marrow mesenchymal stem cells and endothelial cells. Compared with existing technologies, this invention successfully constructs a novel vascularized bone organoid capable of targeting the Piezo1-Nrf2 signaling axis and possessing anti-endothelial aging and mechanical enhancement functions.

The present invention relates to a bioactive scaffold comprising a silver-loaded antimicrobial composition, which acts as a therapeutic device, especially a scaffold, for wound healing and other applications related to the field of tissue engineering. The antimicrobial composition of the invention mainly comprises at least one absorbable matrix for cell-matrix and cell-cell interactions (a natural polymer), a calcium cross-linking component for forming a fibrin gel in situ, and a nanoemulsion containing colloidal silver nanoparticles as a bioactive compound to help control biofilm and infections in the wound. The structures comprising the antimicrobial composition act as dressings and means for aiding the healing of chronic wounds by assisting in infection management and / or improving the biological activity in chronic wounds.

The invention discloses an engineering method for synchronously inducing photoreceptor cells and ganglion cells to mature based on a bioactive scaffold and application, and belongs to the field of biomedical engineering. Based on a 3D printing technology, a polymer is used as a printing material, and the fiber scaffold is prepared through printing; dropwise adding a GelMA solution to the surface of the fiber scaffold, fully infiltrating, and performing irradiation gelation by an ultraviolet light source to form a fiber gel scaffold; cells dissociated from retina organoid in different culture periods are planted on the scaffold, an induced differentiation culture medium is added, synchronous induction of maturation of photosensitive and ganglion functional cells is achieved, and better functional cell integration and substitution are achieved after the scaffold is transplanted to the retina of a mouse. The invention provides a better functional cell source and a new method for treatment of retinal degenerative diseases.