62 results about "Bioactive scaffold" patented technology

The present invention is based on the discovery that a vascular stent or other implantable medical device can be coated with a biodegradable biocompatible polymer to which is attached a bioligand that specifically captures progenitors of endothelial cells (PECs) from the circulating blood to promote endogenous formation of healthy endothelium in Type II diabetics. In one embodiment, the bioligand is a peptide that specifically binds to an integrin receptor on PECs. The invention also provides methods for using such vascular stents and other implantable devices to promote vascular healing in Type II diabetics, for example following mechanical intervention.

The present invention is based on the discovery that a vascular stent or other implantable medical device can be coated with a biodegradable biocompatible polymer to which is attached a bioligand that specifically captures progenitors of endothelial cells (PECs) from the circulating blood to promote endogenous formation of healthy endothelium in Type II diabetics. In one embodiment, the bioligand is a peptide that specifically binds to an integrin receptor on PECs. The invention also provides methods for using such vascular stents and other implantable devices to promote vascular healing in Type II diabetics, for example following mechanical intervention.

The invention provides a method for engraving (three-dimensional) bionic artificial bones in compound bioactive material microdomains. The method comprises the following steps: selecting orthopedics department hard biological materials and active cell tissues for growing support materials, carrying out bionics matching, and carrying out 3DMAX creation and 3D printing by adopting computer three-dimensional design, thus realizing the simulation of artificial bone materials and structures; establishing an artificial bone microcirculation structure and a blood perfusion condition; establishing osteogenesis stem cells growing environments, namely a capillary bed and a biological activity support. The capillary bed and the biological activity support manufactured by adopting the method have the advantages that the initiative response of hard materials (transplanted bones) and host bones is established, the problem of the initiative fusion between currently various biological materials comprising artificial bone materials of allogeneic bones and the host bones is solved, the clinical indication range of bone transplantation is widened, the secondary replacement of artificial joints can be avoided, and the application ranges of the artificial bones and the artificial joints can be widened.

Controlled release of biopharmaceutical growth factors from a hydroxyapatite coating on a bioresorbable interference screw used in cruciate ligament reconstruction surgery on a human. Biologically active scaffolds, such as interference bone screws used for ligament fixation, made by growing calcium phosphate-based hydroxyapatite coatings on bioresorbable poly(α-hydroxy ester) scaffolds that provide controlled mineral dissolution and controlled release of bone morphogenetic protein-2. The biologically active scaffold provides improved bioavailability of BMP-2 growth factor that in turn provides enhanced graft-bone healing in the tibial bone tunnel. The coating method uses surface hydrolysis and modified simulated body fluid incubation which does not require solvent or heat and is conducted at room temperature.

The present invention is based on the discovery that a vascular stent or other implantable medical device can be coated with a biodegradable biocompatible polymer to which is attached a bioligand that specifically captures progenitors of endothelial cells (PECs) from the circulating blood to promote endogenous formation of healthy endothelium in Type II diabetics. In one embodiment, the bioligand is a peptide that specifically binds to an integrin receptor on PECs. The invention also provides methods for using such vascular stents and other implantable devices to promote vascular healing in Type II diabetics, for example following mechanical intervention.

The invention discloses a bioactivity bracket and a preparation method thereof. The bioactivity bracket is obtained through the following steps of firstly, loading bone shape generation proteins on ahollow mesoporous hydroxylapatite microsphere through a grafting manner or an adsorbing manner, so as to obtain a drug bearing microsphere, then mixing the drug bearing microsphere into a bracket matrix solution, then performing printing through a 3D printing technique to prepare a compound bracket, and finally, adsorbing chemotactic growth factors on the surface to obtain the bioactivity bracket;or firstly, blending the drug bearing microsphere and the chemotactic growth factors in the bracket matrix solution, and then performing printing through the 3D printing technique to prepare the bioactivity bracket. Through the adoption of the method disclosed by the invention, the obtained bioactivity bracket can realize time sequence releasing of various growth factors: the chemotactic growth factors are released in an early stage, and bone repair cells are recruited to a damaging region; and bone shape generation proteins are mainly released in the later stage, and bone marrow substrate stem cells which are recruited to the damaging region are induced to be subjected to osteogenic differentiation, so that regeneration and repairing potential of an autologous tissue is sufficiently mobilized, and regeneration and repairing of bone defect are promoted.

Provided are tissue scaffolds colonized by vertebrate cells expressing a transgenic bioactive molecule, where the vertebrate cells are unable to undergo mitosis. Also provided are methods of growing tissue in a mammal and methods of delivering a transgenic bioactive molecule to a tissue of a mammal, using the tissue scaffolds. Additionally, methods of making the tissue scaffolds are provided.

The invention discloses a biological absorbable three-dimensional printing strontium-contained mesoporous bioactive glass support and belongs to the field of biological medical materials. The bone tissue engineering support is prepared from strontium(Sr)-contained mesoporous bioactive glass (Sr-MBG) and polycaprolactone (PCL), wherein the weight proportion of the Sr-MBG to the PCL is 70% and 30%, and 5% of Ca in the Sr-MBG is replaced by the Sr. The bone tissue engineer support is prepared by a three-dimensional printing technique, the two materials of the Sr-MBG and the PCL are printed into the cuboid-shaped bioactive support layer by layer, the layers are formed by criss-cross perpendicular lines, and the holes formed by the perpendicular lines are in square shapes and is favorable for bone cells and new vessels to grow into. The biological absorbable three-dimensional printing strontium-contained mesoporous bioactive glass support can be applied to bone defect repair and treatment, has good biocompatibility and better osteogenic improving capacity, can be absorbed and degraded by living organisms and is suitable for serving as an ideal support material in bone tissue engineering.

The invention relates to a bone tissue engineering stent and a preparation method thereof. The bone tissue engineering stent is prepared from PLGA, isoniazide, rifampicin, allogeneic bone power and bone morphogenetic protein at the weight ratio of (87-51) to (1-6) to (1-6) to (10-30) to (1-7); according to the bone tissue engineering stent, polylactic acid-hydroxyacetic acid material, allogeneic bone power, antituberculosis drugs and bone morphogenetic protein are combined by adopting a 3D printing technology, a bioactive stent is manufactured by adopting the 3D printing technology, the physical, chemical and biological properties of the stent are detected, and the bone tissue engineering stent has the good biocompatibility and the good bone-formation promotion capacity and is biodegradable.

The invention provides a porous microsphere bracket material capable of regulating and controlling the directionally-accumulation biological activity of chondrocytes and a preparation method of the porous microsphere bracket material. The preparation method can be applied to preparation of substitutes for chondrocyte accumulation in a embryonic development process and in a clinical cartilage defect recovery process, and can be applied to the construction of biological active bracket materials in the fields of cartilage tissue recovery and the like. According to the method, active porous microspheres are prepared in the following steps of preparation of porous polyester high-molecule microspheres, chemical crosslinking and drying. The microspheres obtained by the method are high in biocompatibility and degradability and rational in physicochemical property and in microscopic topology morphology, and have porous structures with excellent connectivity and biological activity factor concentration gradient from inside to outside, thus being capable of regulating and controlling the directional chondrocyte accumulation in vitro, providing a good carrier for the in-vitro function expression of the chondrocytes, and serving as a good biological bracket material for tissue engineering cartilage.

A device for inhibiting adhesion of apposing human body tissue layers includes a scaffold having a designated mean pore size, relative density, and degradation half-life. The scaffold may be operably positioned between apposing tissue layers, such as proximate adhesiogenic layers at a wound site, so as to permit remesothelialization of the tissue without formation of fibrous adhesions. The scaffold device of the invention inhibits adhesion formation by promoting contractile cell migration away from the wound site for a predetermined period of time. The invention further relates to device and methods for promoting internal tissue regeneration, and for provision and / or dispensation of therapeutic and / or diagnostic agents in vivo.

The invention relates to a preparation method of a double-layer composite scaffold for inducing regeneration of dental pulp and dentin tissues. The preparation method comprises the following steps: (1) preparing bioactive glass (PSC) synthesized by taking phytic acid as a precursor; (2) performing polypeptide modification of PSC; (3) preparing an outer layer part of the scaffold; (4) preparing aninner layer part of the scaffold; and (5) performing completing crosslinking of the double-layer scaffold: soaking the scaffold obtained in the step (4) into 0.1-0.5% of a glutaraldehyde crosslinkingagent to crosslink the inner-layer scaffold and the outer-layer scaffold to form the composite scaffold with a double-layer structure. The bioactive scaffold material has layered induction capability,conforms to the root canal form of teeth and is used for inducing regeneration of dental pulp and dentin composite tissues; the inner layer of the scaffold can chemotactic stem cells to migrate and climb in, so that generation of dental pulp-like soft tissues is facilitated; the scaffold outer layer can further induce cell dentin differentiation and promote generation of dentin-like hard tissues;finally, special soft and hard tissues similar to natural teeth are formed.

A bioactive scaffold for cell delivery to a wound includes a degradable body portion configured to be placed within a wound bed, the body portion including a least one extracellular matrix biomaterial.

The invention discloses a novel bioactive bracket with a chemotactic function, which is used for endogenous cell migration, and promotion of endogenous cells to survive and differentiate for repairing spinal cord injury, and has a rear nerve protection effect. The preparation method comprises the following steps: by taking natural silk-fibroin extracted from silkworm cocoon as a medium, transforming a neurotrophic factor-3(NT-3), retinoic acid (RA) and a silk-fibroin crystalline form by utilizing a freeze-drying technology and an alcohol cross-linking agent, cross-linking and fixing in a three-dimensional gelatin sponge bracket. The bioactive bracket with the chemotactic function provided by the invention can release the chemotactic factor to attract endogenous cell migration to an injury / transplantation area with a relatively long time after being transplanted to spinal marrow, and can release a nutritional factor with a nerve protection effect to improve a microenvironment, so that the purpose of attracting endogenous cells to perform self-repair of the spinal cord injury is achieved.

The invention relates to field of regeneration medical science and organization engineering, and provides an implantable engineering liver implant and construction method thereof. The method includes steps of employing hydrogel material as biological activity support, compounding slow release microsphere containing angiogenesis cell factor and liver cell induction factor (and biological activity substance), compounding liver cell, constructing engineering liver cell / support compound, promoting propagation of liver cell and blood vascularization of engineering liver implant, and after the liver cell / support compound is changed to gel, thrashing the gel to liver implant unit. By implanting the liver implant unit to liver outer site or implanting multi-site to liver, the invention overcomes ischemic problem of engineering liver organization in initial stage of implantation, effectively solves problem of regeneration of engineering liver organization in vivo, and provides a new method for treating various hepatopathies by employing organization engineering method to construct engineering liver organization in vivo.

The invention discloses a three-dimensional printing strontium-containing mesoporous bioglass bracket loaded with soy isoflavone, and belongs to the field of the biomedical material. The bracket is composed of the strontium-containing mesoporous bioglass (Sr-MBG), polycaprolactone (PCL) and soy isoflavone, wherein the quality proportions of the Sr-MBG and PCL are 70% and 30% respectively, and 5% of Ca in the Sr-MBG is replaced by Sr. The soy isoflavone is loaded into the mesoporous of the Sr-MBG, two materials of the Sr-MBG loaded with the soy isoflavone and the PCL are printed to be the cubical biological activity bracket layer by layer by using the three-dimensional printing technology, each layer is formed by crisscrossed perpendicular lines, and the pore formed by the perpendicular lines is a square. The bracket can be applied to the repair and treatment in allusion to the bone defect, has good biocompatibility and better capacity for promoting osteogenesis and can be absorbed and degraded by the living organism. The treatment effect for the bone defect of the osteoporosis patient can be enhanced after the soy isoflavone is loaded, and the bracket is the perfect bracket material for bone tissue engineering.

The invention relates to a bioactive bracket with a bionic lotus-root structure, and a preparation method and an application thereof. The bioactive bracket is formed by stacking of compositional elements, wherein each compositional element is internally provided with one or more parallel channels. The bioactive bracket has the bionic lotus-root structure, i.e., the element is internally provided with parallel multi-channel structures, so that the porosity and the specific surface area of the biological material are greatly improved, more benefit for adhesion and multiplication of cells and bone formation and vessel formation in a body is achieved, and the application to cell transmission and restoration and regeneration of massive bone defects can be achieved.

The present invention is based on the discovery that a vascular stent or other implantable medical device can be coated with a biodegradable biocompatible polymer to which is attached a bioligand that specifically captures progenitors of endothelial cells (PECs) from the circulating blood to promote endogenous formation of healthy endothelium in Type II diabetics. In one embodiment, the bioligand is a peptide that specifically binds to an integrin receptor on PECs. The invention also provides methods for using such vascular stents and other implantable devices to promote vascular healing in Type II diabetics, for example following mechanical intervention.

The invention discloses a composite biology bracket material for repairing bone defects, and belongs to the technical field of bone tissue engineering. Reorganized TG2 gland viruses are transfected toEMSCs having multi-direction differentiation potency, in vitro assessment is performed on influence of TG2-EMSCs on osteogenesis of a fibrin bracket, and a bioactivity bracket containing TG2-EMSCs istransplanted into SD rats with skull defects, and the bone defect capacity is detected. Results prove that the fibrin bracket containing TG2-EMSCs is used for performing transplanting treatment on the skull defects, 55% of the damaged regions can be healed within two weeks, and the fibrin bracket containing natural EMSCs prove that 17% of the damaged regions are healed within the same time. The biology bracket is high and stable in biocompatibility, low in cost and simple and convenient to operate, and therefore, the fibrin bracket containing TG2-EMSCs has important clinical application valueto repair of the skull defects.

Provided is a method of preparing a bioactive scaffold for inducing tendon regeneration, the method includes decellularizing a fresh tendon tissue and to obtain a decellularized tendon sheet scaffold or slice scaffold, and adding ECM materials to the decellularized tendon sheet scaffold or slice scaffold.

The invention relates to a bioactive stent of a hotdog-like structure and a preparation method and application thereof. The bioactive stent comprises a hollow tube made from a bioactive material and arod made from the bioactive material and located in the hollow tube made from the bioactive material, and the rod made from the bioactive material has a continuous and layered structure.

The invention relates to a calcium phosphate ceramic active scaffold capable of regulating and controlling cell adhesion performance. According to the method, the calcium phosphate micro-nano active coating is individually designed on the surface of the calcium phosphate biological ceramic scaffold, so that the adhesion behavior of cells is accurately regulated and controlled, and the repair process of tissues is regulated and controlled. According to the preparation method, the calcium phosphate ceramic bracket can be obtained through processes such as 3D printing, a foaming method and a tabletting method, and the surface calcium-phosphorus micro-nano active coating is obtained through processes such as hydrothermal treatment, mineralization and electro-deposition. The design accurately acts on the adhesion protein of the cells at the micro-nano level, so that the change of the cell adhesion behavior is realized. According to the invention, the regulation and control effects of the material on adhesion, activity, proliferation and differentiation of cells are obviously enhanced, and the material has higher hydrophilicity, protein adsorbability, stem cell collection and tissue repair promotion capabilities.

The invention discloses a collagen composite bioactive stent and a preparation method thereof. The collagen composite bioactive stent is prepared through the steps that I-type rat tail collagen is dissolved in an acetic acid solution, a DME / F12 culture medium and a NaOH solution are added to prepare an I-type rat tail collagen solution, CSCI and beta-sodium glycerophosphate are dissolved in the DME / F12 culture medium to prepare a temperature-sensitive CSCI solution, and the I-type rat tail collagen solution is mixed with the temperature-sensitive CSCI solution. The collagen composite bioactivestent can be used for treating myocardial infarction ischemic heart diseases by being taken as tissue engineered myocardium to be injected into a myocardial ischemia area.

The invention provides a preparation method of a bone defect repair stent, which comprises the following steps of: obtaining imaging data of a bone defect part through modes of clinical imaging CT (Computed Tomography), MRI (Magnetic Resonance Imaging) scanning and the like, then modeling by utilizing CAD (Computer Aided Design) software according to the specific form of the bone defect of a patient, outputting an STL (Standard Template Library) format file of the bone tissue repair stent, and carrying out A biodegradable material photocuring polycaprolactone is used as a printing raw material, a photoetching 3D printer is used for stacking and forming the material, and the porous bone repair scaffold suitable for the form of a defected part of a patient is printed. And grafting the biotinylated vesicles to the surface of the scaffold by using a biotin-avidin system to construct the bioactive scaffold with the surface loaded with the stem cell vesicles. According to the invention, the stem cell vesicles are loaded on the photocuring printed porous bone scaffold by using a biotin-avidin system, so that the personalized customization of the bone scaffold can be realized, and the scaffold has the effect of accelerating bone defect repair by using active substances in the stem cell vesicles.