Preparation method of double-layer composite scaffold for inducing regeneration of dental pulp and dentin tissues

Abstract

The invention relates to a preparation method of a double-layer composite scaffold for inducing regeneration of dental pulp and dentin tissues. The preparation method comprises the following steps: (1) preparing bioactive glass (PSC) synthesized by taking phytic acid as a precursor; (2) performing polypeptide modification of PSC; (3) preparing an outer layer part of the scaffold; (4) preparing aninner layer part of the scaffold; and (5) performing completing crosslinking of the double-layer scaffold: soaking the scaffold obtained in the step (4) into 0.1-0.5% of a glutaraldehyde crosslinkingagent to crosslink the inner-layer scaffold and the outer-layer scaffold to form the composite scaffold with a double-layer structure. The bioactive scaffold material has layered induction capability,conforms to the root canal form of teeth and is used for inducing regeneration of dental pulp and dentin composite tissues; the inner layer of the scaffold can chemotactic stem cells to migrate and climb in, so that generation of dental pulp-like soft tissues is facilitated; the scaffold outer layer can further induce cell dentin differentiation and promote generation of dentin-like hard tissues;finally, special soft and hard tissues similar to natural teeth are formed.

Description

technical field

[0001] The invention relates to the technical field of biomedical materials, in particular to a method for preparing a double-layer composite support for inducing pulp dentin tissue regeneration. Background technique

[0002] Human teeth are susceptible to caries, trauma, or occlusal trauma, which can cause damage to the pulp and dentin. Pulp damage is difficult to reverse and is prone to necrosis. At present, the main method of clinical treatment is root canal therapy, which is to use inert material to tightly fill the root canal after debridement. Restricted by the complex anatomical structure of the root canal system, root canal treatment techniques are complex and cost a lot of social medical costs; mechanical and chemical drug debridement is required, which is potentially harmful to the periapical tissue. The important thing is that after losing the pulp, the tooth cannot continue to form secondary dentin and tertiary dentin, and loses the ability to se...

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