The deciduous tooth pulp stem cell low-temperature preservation box comprises a bottom plate, a lifting mechanism is arranged on the upper surface of the bottom plate, a box body is arranged on the outer side of the lifting mechanism, the lower side of the box body is fixedly connected with the position, close to the side edge, of the upper surface of the bottom plate, and a preservation box body is connected to the upper side of the lifting mechanism. An opening matched with the preservation box is formed in the upper side wall of the box body, a mounting cavity is formed in the left side of the interior of the preservation box, a refrigeration mechanism is arranged in the mounting cavity, a storage cavity is formed in the right side of the interior of the preservation box, a placement frame is arranged in the storage cavity, and a plurality of test tube insertion openings are formed in the placement frame; the upper side of the storage cavity is provided with a cover body, the cover body is slidably mounted on the upper side of the storage cavity, the height of the storage box can be adjusted, so that the device is suitable for doctors with different heights, the doctors can work more conveniently, meanwhile, the refrigeration mechanism refrigerates the interior of the mounting cavity, and it is guaranteed that teeth are stored in a low-temperature state.