The invention relates to a selection and culture method of excellent breed large-tooth flounder featuring fast growth and high survival rate. The technical method comprises: culture and reproduction regulation and control of large-tooth flounder breeding group, establishment of family by sperm freezing technology, culture and standardization of the family, determination and analysis of number and properties, estimation of genetic parameters, screening of excellent family, and establishment of excellent breed by inducing thelykaryon of large-tooth flounder to develop by using heterologous frozen sperms. The invention can select and culture new breed of cultured large-tooth flounder featuring fast growth and high survival rate, screen out four excellent large-tooth flounder families featuring fast growth and high survival rate (0703, 0719, 0750 and 0751), and establish seven excellent breeds of large-tooth flounder developed from thelykaryon (0920, 0921, 0927, 0938, 0939, 0946 and 0947). The technical method can be applied to new variety culture of large-tooth flounder cultivation, solve the problems of culturing large-tooth flounder fish in sea water such as slow growth and low survival rate, and enhances economic benefit of large-tooth flounder cultivation.