57 results about "Deciduous tooth" patented technology

The present invention provides a tooth correctional system suitable for mixed dentition and a manufacturing method of the tooth correctional system, wherein the method comprises the following steps: building an original dentition digital model representing the original tooth state which includes at least one embedded tooth; acquiring a corresponding eruption tooth standard model for each embedded tooth in the original tooth state to obtain a mixed dentition digital model; producing a series of target dentition digital models according to the mixed dentition digital model; and manufacturing a tooth correctional device according to the produced series of target dentition digital models. The tooth correctional system can be used for correction of teeth of patients of any ages, and is especially suitable for dental transitional period adolescent patients simultaneously with deciduous teeth and permanent teeth.

The invention relates to the field of stem cells and discloses a culture method of deciduous tooth pulp stem cells. The culture method includes: taking pulp tissue, cutting the pulp tissue into pieces, and then adding I-type collagenase; digesting for 10-20min under conditions of 37 DEG C and 200rpm; stopping digestion of a serum-free culture medium; blowing and beating discrete cell mass to obtain single discrete cells; adding cell suspension to re-suspend the cells, and adjusting cell density; culturing the cells in a carbon dioxide incubator at temperature of 37 DEG C and with humidity of 95%; when the cells grow to be fused by 80-90%, using digestive liquid of trypsase to digest the cells before passage culture, wherein the cell suspension is composed of the serum-free culture medium, epidermal growth factor and basic fibroblast growth factor. The cells cultured by the method are good in shape, have tendency of fusiform cluster growing and are high in activity and quick in proliferation, good stem cell characteristics of the deciduous tooth pulp stem cells can be maintained, and the culture method is suitable for large-scale culture of the deciduous tooth pulp stem cells.

The invention discloses stem cells for specifically expressing a programmed death receptor 1 (PD-1), and particularly relates to the stem cells from an organic cavity. The stem cells comprise but notlimited to dental pulp stem cells, gingival mesenchymal steam cells (GMSCs), periodontal ligament stem cells (PDLSCs), stem cells of apical papilla (SCAPs) and dental follicle stem cells (DFSCs) or any combination thereof, and preferably comprise stem cells from human exfoliated deciduous teeth (SHED) and/or dental pulp mesenchymal stem cells for permanent teeth (DPSC); the stem cells also includemesenchymal stem cells from other tissues, which do not express the PD-1 at first and express the PD-1 after being modified by CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), suchas PD-1 plus bone mesenchymal stem cells (BMMSC) modified by the CRISPR. The invention further discloses an identifying and separating method for the stem cells for specifically expressing the PD-1,a method for preparing the PD-1 plus mesenchymal stem cells modified by the CRISPR as well as application of the mesenchymal stem cells for expressing the PD-1, disclosed by the invention, in tissue regeneration, pain relief and treatment of chronic pain and a series of diseases.

The invention discloses a tooth grinding biscuit making method and belongs to the technical field of food processing. The tooth grinding biscuit making method comprises the following steps of uniformly mixing pumpkin powder, corn flour and wheat flour of raw materials according to a certain ratio; placing the above into a dough maker to kneading dough for 1 minute to 5 minutes; adding eggs, white granulate sugar, malt sugar, cream, ammonium bicarbonate, salt and baking soda into the dough make according to a certain ratio; continuously kneading dough for 10 minutes to 15 minutes to make a paste and standing the paste to ferment for 15 minutes to 20 minutes; placing the paste into a mold, putting the mold into an oven under the temperature of from 175DEG C to 180 DEG C and obtaining biscuits after baking for 20minutes to 30 minutes, wherein the content of wet gluten in the wheat flour is from 15% to 20%. The tooth grinding biscuits obtained from the tooth grinding biscuit making method is appropriate in hardness, not easy to break and scale off and applicable for tooth grinding of infants during eruption of deciduous teeth.

The invention belongs to the field of medical instruments and relates to a use method of resin, a use method of polyether-ether-ketone and a manufacturing method of a deciduous tooth preformed crown. The resin is used as the material of the deciduous tooth preformed crown; the polyether-ether-ketone is used as a material of the deciduous tooth preformed crown. The manufacturing method of the deciduous tooth preformed crown comprises the following steps of: S1, manufacturing a digital model of the deciduous tooth preformed crown; S2, according to the digital model, performing digital cutting on an initial material to obtain a semi-finished product of the deciduous tooth preformed crown; and S3, polishing the surface of the semi-finished product of the deciduous tooth preformed crown to obtain a finished product of the deciduous tooth preformed crown. According to the manufacturing method of the deciduous tooth preformed crown of the invention, the3D digital model of the deciduous tooth preformed crown isestablished; and 3D printing is performed to obtain the deciduous tooth preformed crown according to the parameters of the 3D digital model. According to the use method of the resin, the use method of the polyether-ether-ketone and the manufacturing method of the deciduous tooth preformed crown of the invention, deciduous teeth, the occlusion function and the growth and development of an oral-maxillofacial region are ensured.

The invention provides a serum-free complete medium and a use thereof. The serum-free complete medium comprises two mediums which are orderly used or used together. The medium I contains L-glutamine with the concentration of 2mM/L and 1-4% of a serum substitute for effectively promoting cell adhesion and extension based on a basic medium. The medium II contains L-glutamine with the concentration of 2mM/L and 7-10% of a serum substitute for culturing ESC and iPSC based on a basic medium. The invention also provides the use of the serum-free complete medium in culture of mesenchymal stem cells.The serum-free complete medium utilizes simple medium ingredients and is easy to prepare. The mesenchymal stem cell culture method is conducive to in-vitro culture of human deciduous teeth mesenchymalstem cells and keeps the dryness and multi-directional differentiation ability of stem cells.

The invention discloses a novel membrane-pressing type space maintainer which comprises jaw pads, wherein premature-loss teeth resin teeth are arranged between the jaw pads; a membrane base support is arranged on the jaw pads in such a situation that spaces of the resin teeth and premature-loss teeth are close to base teeth, and the positions, relatively adjacent to the buccal labial surfaces of the base teeth, of the membrane are respectively provided with retaining resin bumps; and grooves which are embossed on corresponding rectangular, elliptic and circular resin parts on the base teeth buccal labial surfaces on the membrane and are in corresponding shapes are united with the retaining resin bumps. The space maintainer disclosed by the invention solves a series of problems of being poor in retaining effect, easy to become loose and fallen, strong in foreign body sensation, failing to recover a certain chewing efficiency, difficult in cleaning, easy to cause carious lesion and the like in a space maintainer for children suffering premature shedding of deciduous teeth.

The invention discloses a method for realizing separation and in-vitro proliferation of stem cells from exfoliated deciduous teeth. The method comprises the steps of tooth selection, deciduous tooth cutting, separation of the stem cells from the deciduous teeth, culture and proliferation of the stem cells from the deciduous teeth, and the like. The method has the beneficial effects that the purified stem cells from the human exfoliated deciduous teeth through the method can differentiate towards the specific cell types including osteoblasts, lipoblasts, neurocytes and the like under certain conditions, and thus wide space is provided for researching dental pulp stem cells.

The invention discloses a preparation method of a dental pulp stem cell polymer for exfoliating deciduous teeth, the dental pulp stem cell polymer prepared by the preparation method and an applicationof the dental pulp stem cell polymer in preparation of a preparation for permanent tooth reimplantation. The preparation method comprises the following steps: (1) cleaning dental pulp tissue from exfoliated deciduous teeth, (2) digesting the dental pulp tissue by collagenase I and Dispase to obtain a cell suspension, (3) adding a VC culture solution and culturing to obtain a dental pulp stem cellpolymer. The dental pulp stem cells of exfoliated deciduous teeth not only have the characteristics of mesenchymal stem cells, but also express related genes of embryonic stem cells, have higher induced differentiation capacity than the dental pulp stem cells, and have ideal effects on dental pulp regeneration and periodontal healing of completely-dislocated young permanent teeth.

The invention discloses an absorbable polylactic acid film for enhancing airtightness of an absorbable root canal post of a deciduous tooth. The absorbable polylactic acid film is prepared by dissolving the following raw materials in parts by weight: 0.5-1.5 parts of polylactic acid, and 5-15 parts of solvent. The absorbable polylactic acid film and the absorbable root canal post are made of the same material, and the absorbable polylactic acid film has no influence on human dental follicle cells, deciduous periodontal ligament cells and deciduous dental pulp cells, cannot result in apoptosis of the human dental follicle cells, is nontoxic, can effectively control the effective strength of the absorbable root canal post, can recover functions and a shape of the deciduous tooth for a long time to the greatest extent, and does not influence normal replacement of deciduous permanent teeth. A preparation method of the film is simple and practicable, and can be used for manufacturing during clinical application. The film is used for residual root and residual crown repair of the deciduous tooth, a crown restoration is good in retention postoperatively, and the film meets the clinical requirement of pediatric dentistry.

The invention provides a convenient deciduous tooth extraction auxiliary device based on medical diagnosis and treatment of young children, relates to the technical field of medical instruments, and aims to solve the following problems: when the existing deciduous tooth extraction auxiliary device is used, deciduous tooth extraction easily causes head lowering of a child due to pain, so that the head of the child easily collides with the auxiliary device; and furthermore, the child easily tends to cover the oral cavity by using the hand, so that bacteria enters the oral cavity. The convenientdeciduous tooth extraction auxiliary device comprises a main body and an overturning part; the main body is of a T-shaped plate structure; furthermore, the main body is made of a relatively light andhard material; the overturning part is installed at the right end of the main body; a connecting rod of the overturning part is connected with the left end of the main body through a connecting shaft;a buffer part is used for being mounted in a mounting groove, so that limiting of the buffer part can be released at the same time when a moving block is released from limiting and moves downwards toextract teeth; therefore, the buffer part can move outwards, so that the buffer part can be supported by a spring to pop up; and thus, a child can make contact with the buffer part when feeling painful and lowering the head.

The invention provides a marker for distinguishing gingival stem cells and deciduous tooth pulp stem cells and a distinguishing method thereof. According to the method, ANXA2 is used as the marker. The identification method is convenient and quick, is low in cost, has a relatively high reference value for distinguishing of the gingival stem cells and the deciduous tooth pulp stem cells and dentaltissue engineering, and is suitable for promotion and application.

The invention discloses a modeling method of a digital child oral treatment operation model. Acquiring specimen CBCT data, and scanning the specimen by using a cone beam CT to acquire image information of dentition on the specimen, the image information recording information such as three-dimensional position of each tooth in the dentition; separating the tooth specimen and acquiring microscopic CT data of all the teeth, separating all the teeth from the specimen, and then performing microscopic CT scanning one by one to acquire high-precision imaging information of each tooth; and carrying out three-dimensional reconstruction on the teeth and dentition, and carrying out three-dimensional reconstruction on the two kinds of image data by using software of a software medical image control system. The method is reasonable in design and simple to operate, various types of deciduous tooth models can be constructed on the basis of an original model, teaching and training of deciduous tooth related treatment operation are greatly facilitated, and meanwhile, the precision of the deciduous tooth models in the modeling process can be effectively improved through cooperation of various modeling software.

The invention relates to a method for immortalizing a human exfoliated deciduous tooth endodontium stem cell line through hTERT recombinant lentivirus. The method comprises: 1, carrying out primary culture on primary human exfoliated deciduous tooth endodontium stem cells SHED; and 2, constructing immortalized human exfoliated deciduous tooth endodontium stem cells hTERT-SHED stably expressing hTERT gene. According to the present invention, the constructed immortalized genetically engineered cells expressing the hTERT gene is adopted as the effective cell model for researching the biological characteristics of the human exfoliated deciduous tooth endodontium stem cells so as to easily and deeply research the applications of the constructed immortalized genetically engineered cells as the seed cells in the periodontal tissue engineering and the actions of different drugs on the cells and the signal transduction pathways of the related action mechanisms, and the constructed immortalized genetically engineered cells are used to screen the traditional Chinese medicine effective components for effectively treating chronic periodontitis so as to provide the powerful tool for the subsequent research of the periodontal tissue repair and reconstruction and the development of the novel effective drugs.

The invention discloses use of mesenchymal stem cells of dental pulp in obtaining of an artificial spinal cord and a preparation method for the artificial spinal cord. The preparation method consists of the following steps: (1) acquiring healthy deciduous teeth removed due to deformity correcting, digesting the healthy deciduous teeth with type 1 collagenase, inoculating a culture flask with the digested substance, carrying out washing to discard nonadherent cells, re-suspending adherent cells, then, carrying out culture, so as to obtain the mesenchymal stem cells of the dental pulp; (2) culturing the mesenchymal stem cells of the dental pulp with an alpha-MEM culture medium, so as to propagate the mesenchymal stem cells of the dental pulp; and (3) selecting third-generation dental-pulp mesenchymal stem cells, culturing the dental-pulp mesenchymal stem cells with an alpha-MEM culture medium containing vitamin C when the cell fusion rate is 90%so as to obtain a white membrane-shaped structure, and carrying out in-vitro molding, thereby finally obtaining the artificial spinal cord with a three-dimensional structure. The artificial spinal cord obtained by the preparation method can be used for inhibiting cicatrization and inflammation and promoting axon regeneration; and among rats suffering from complete spinal injury, Spinor therapy causes remarkable movement improvement, perception recovery and faster urination reflection recovery.

The invention discloses a method for preparing dental pulp mesenchymal stem cells. The method includes the following steps of S1, collecting deciduous tooth tissues; S2, transferring the collected deciduous tooth tissues to a laboratory under low temperature conditions; S3, using hydraulic forceps to squeezing and crushing the deciduous tooth tissues; S4, separating dental pulp from the broken deciduous tooth tissues; S5, putting the separated dental pulp into a laboratory small masher for mashing treatment; and S6, performing amplified culturing on dental pulp stem cells. According to the method for preparing the dental pulp mesenchymal stem cells, the hydraulic forceps are adopted to crush the deciduous tooth tissues, the deciduous tooth tissues are squeezed and crushed, and the dental pulp is mashed, and therefore the operation is convenient, the efficiency is high, the raw materials are saved, and the extraction success rate of the stem cells is high.

The present invention relates to a method of raising funds for an organization. In one embodiment, the organization distributes a solicitation to a buyer, or for delivery to a buyer by way of a child relative of the buyer. The solicitation comprises an offer to sell a jewelry article made from a processed exfoliated deciduous tooth of the child, set in a jewelry object, as a gemstone would be set. The buyer sends a payment for the jewelry article to the manufacturer, who sends a payment to the organization for having distributed a solicitation that was converted into a purchase. The buyer sends the exfoliated deciduous tooth to the manufacturer. The deciduous tooth is specially processed in a manner that allows it to be set in the buyer's selected jewelry object as a gemstone would be set to create the completed jewelry article.

The invention discloses a 3D printed tooth root stent for alveolar bone site preservation, and a preparation method thereof. The 3D printed tooth root stent for alveolar bone site preservation is of ahollow three-dimensional communication structure; a main body material is beta-tricalcium phosphate; stem cells from human exfoliated deciduous teeth are adhered to the surface; and osteogenic induction is carried out. According to the invention, advantages of a biological ceramic material (beta-tricalcium phosphate), the stem cells and a 3D printing technology are combined and jointly applied tosite preservation; the 3D printed TCP stent has good biological characteristics; furthermore, the stem cells from human exfoliated deciduous teeth can serve as good bone tissue engineering seed cellsloaded on the stent; the stem cells from human exfoliated deciduous teeth are combined with the TCP stent material; therefore, the horizontal width of an alveolar bone and the vertical height of an alveolar ridge buccal bone plate after tooth extraction can be effectively preserved; and the formation of new bones in alveolar fossa can be promoted.

The invention discloses a method for improving diabetes mellitus by dental pulp mesenchymal stem cells. The method comprises the following steps: firstly, preparing dental pulp mesenchymal stem cells,wherein pulling out deciduous teeth by a dentist, transporting the teeth to a cell preparation center in a cold storage state through cold chain transportation, then cutting the teeth open in a laboratory under a GMP condition to extract dental pulp tissues in the teeth, and extracting stem cells in dental pulp; culturing the extracted dental pulp stem cells in a culture bottle for passage and amplification; carrying out reinfusion of the prepared dental pulp mesenchymal stem cells into the body of a diabetic patient through intravenous injection, wherein 500,000-700,000 units/kg are injectedonce every 20 days, the stem cells are injected for 3-4 times in total, the use effect of the patient is continuously observed, and whether the treatment needs to be continuously adopted for improvement or not is subsequently determined. The pancreas islet tissue proliferation and regeneration are promoted, insulin secretion is improved, blood sugar is reduced, immune factor disorder is corrected, and inflammation is relieved. The exfoliated deciduous tooth stem cells may be used to ameliorate type 2 diabetes and type 1 diabetes.

The deciduous tooth pulp stem cell low-temperature preservation box comprises a bottom plate, a lifting mechanism is arranged on the upper surface of the bottom plate, a box body is arranged on the outer side of the lifting mechanism, the lower side of the box body is fixedly connected with the position, close to the side edge, of the upper surface of the bottom plate, and a preservation box body is connected to the upper side of the lifting mechanism. An opening matched with the preservation box is formed in the upper side wall of the box body, a mounting cavity is formed in the left side of the interior of the preservation box, a refrigeration mechanism is arranged in the mounting cavity, a storage cavity is formed in the right side of the interior of the preservation box, a placement frame is arranged in the storage cavity, and a plurality of test tube insertion openings are formed in the placement frame; the upper side of the storage cavity is provided with a cover body, the cover body is slidably mounted on the upper side of the storage cavity, the height of the storage box can be adjusted, so that the device is suitable for doctors with different heights, the doctors can work more conveniently, meanwhile, the refrigeration mechanism refrigerates the interior of the mounting cavity, and it is guaranteed that teeth are stored in a low-temperature state.

The invention belongs to the technical field of dental preparations, and discloses a deciduous tooth root canal filling material and a preparation method thereof. The deciduous tooth root canal filling material is composed of 30-50% by volume of calcium hydroxide, 10-20% by volume of iodoform and 30-50% by volume of Vaseline. The preparation method specifically comprises the following steps: weighing and extracting calcium hydroxide and iodoform powder according to a volume ratio; weighing the certain amount of medical Vaseline; and uniformly mixing the calcium hydroxide, the iodoform powder and the medical Vaseline at normal temperature, fully stirring to form a paste, and uniformly stirring the paste to obtain the deciduous tooth root canal filling material. The deciduous tooth root canal filling material belongs to an iodoform and calcium hydroxide composite preparation; and the adhesion of the root canal wall is further improved through material proportioning, and the material absorption rate is reduced.