56 results about "Osseous Differentiation" patented technology

The invention discloses an application of an AMPK (adenosine monophosphate kinase) activator or a PPAR (peroxisome proliferator activated receptor) gamma inhibitor in preparation of a medicine for preventing or treating diabetic osteoporosis. The invention discloses a key effect of a brand new signal channel, namely an AMPK-PPAR gamma-ERS axis, in osteogenic differentiation disorder of diabetes mellitus, and provides a method for preventing and treating diabetic osteoporosis by regulating and controlling the signal axis by taking metformin as a core. The invention provides new guidance for research and development of medicines for treating osteoporosis, and has potential clinical application and mechanism research values.

The invention discloses long-acting high-dynamic hydrazone bond hydrogel as well as a preparation method and application thereof, and particularly relates to the technical field of biomedical materials. The preparation method comprises the following steps: taking 1-adamantane acetic acid and adipic dihydrazide modified sodium hyaluronate and benzaldehyde-terminated eight-arm polyethylene glycol as carrier raw materials; the hydrazone bond hydrogel with relatively high stability is prepared through a coupling reaction of an aldehyde group of benzaldehyde-terminated eight-arm polyethylene glycol and a hydrazide group of 1-adamantane acetic acid and adipic dihydrazide modified sodium hyaluronate. The beta-cyclodextrin modified m-phenylenediamine catalyst is dynamically combined into the carrier through reversible host-guest complexation between beta-cyclodextrin and 1-adamantane acetic acid, so that the long-acting high-dynamic hydrazone bond hydrogel is obtained, and the requirement of three-dimensional culture of stem cells on mechanical properties can be met; and network dynamics can be maintained for a long time, so that stem cell spreading is supported, mechanical signal transduction is activated, osteogenesis-related gene expression is promoted, and stem cell osteogenesis differentiation capability is enhanced.

The invention provides a mesenchymal stem cell composition as well as a preparation method and application thereof in knee joint products, and belongs to the technical field of medicines. The invention relates to application of a mesenchymal stem cell combined naringin derivative in preparation of medicines for treating osteoporosis and repairing knee joint injury and arthritis. The naringin derivative is prepared from naringin through an ether esterification reaction, the metabolic cycle of the naringin derivative is prolonged and the water solubility is improved while the original activity is maintained, so that the free drug concentration and tissue infiltration capacity in bones are improved, the bioavailability of the naringin derivative is improved, and the bioavailability of the naringin derivative is improved. The prepared mesenchymal stem cell composition can induce mesenchymal stem cell osteogenic differentiation and improve bone repair capacity, has a good intervention effect on osteoporosis, has a sustained and controlled release effect and prolongs medicinal time.

The invention provides application of an FZD4 gene. The FZD4 gene is used for regulating osteogenic differentiation and mineralization of chicken bone marrow mesenchymal stem cells. The FZD4 gene in the chicken bone marrow mesenchymal stem cells is over-expressed and is used for promoting osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; or / and the FZD4 gene in the chicken bone marrow mesenchymal stem cells is knocked down to inhibit osteogenic differentiation and mineralization of the chicken bone marrow mesenchymal stem cells; the nucleotide sequence of the coding region of the FZD4 gene is as shown in SEQ ID NO. 1. According to the invention, the positive regulation effect of the FZD4 gene in osteogenic differentiation of chicken bone marrow mesenchymal stem cells is determined for the first time, the blank of a bone regulation mechanism of the poultry FZD4 gene is filled, and a new biological preparation target is provided for prevention and treatment of poultry bone diseases; the FZD4 gene can also be used as a molecular marker for breeding chicken varieties with high bone strength.

The application provides a kind of basic phosphatase induced T1 and T2 signal switching response type magnetic resonance imaging contrast agent and its preparation method and application, the response type magnetic resonance imaging contrast agent includes superparamagnetic nanoparticle, and polyethylene glycol phosphoric acid monoester modified to the surface of superparamagnetic nanoparticle;The superparamagnetic nanoparticle includes ferroferric oxide nanoparticle.The contrast agent of the application can realize T1 and T2 signal switching, is used for stem cell osteogenic differentiation monitoring, not only improves the detection sensitivity of magnetic resonance imaging to microstructure and function, also provides new tools and methods for stem cell treatment in biomedical research and clinical practice.

The application discloses application of microRNA-32-5p in preparation of a medicine for preventing and treating osteoporosis, and a medicine composition taking microRNA-32-5p as an active ingredient. It is proved through experiments that knocking out the microRNA-32-5p gene significantly aggravates osteoporosis induced by estrogen deficiency; the microRNA-32-5p plays a bone protection role by promoting osteogenic differentiation of bone marrow mesenchymal stem cells. Therefore, the microRNA-32-5p can be applied to prevention and treatment of osteoporosis as a microRNA medicine.

The invention discloses a rehmannia neutral homogeneous polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of traditional Chinese medicine preparations. The polysaccharide mainly comprises galactose, glucose and fructose, the molar ratio of the galactose to the glucose to the fructose is 2.72: 1.17: 1.11, and the weight-average molecular weight of the polysaccharide is 2152 Da. The rehmannia neutral polysaccharide can play a role in treating osteoporosis by increasing bone mineralization amount and bone mineral density; the proliferation and directional osteogenic differentiation of the bone marrow mesenchymal stem cells BMSCs can be promoted, and the obvious application effect is achieved in the aspect of preparing the bone defect treatment medicine through the stem cells. The rehmannia neutral homogeneous polysaccharide shows a huge application prospect in the aspects of preparation of medicines for treating osteoporosis and stem cell treatment (especially bone regeneration treatment based on mesenchymal stem cells). A new candidate substance and a research direction are provided for developing an efficient and safe bone metabolism disease treatment strategy derived from traditional Chinese medicines.

This invention belongs to the field of biomedical technology and relates to the application of OSM inhibitors in the preparation of drugs for the prevention or treatment of calcific aortic valve disease. This invention reveals for the first time that OSM drives osteogenic differentiation and calcified nodule formation of valvular interstitial cells through the OSM-OSMR-JAK2 / JAK3-STAT3-RUNX2 signaling axis, which is the core pathogenic mechanism leading to calcific aortic valve disease. This invention utilizes anti-OSM neutralizing antibodies or sulforaphane to effectively block this signaling axis, significantly inhibiting macrophage infiltration, valvular calcification, and improving hemodynamic abnormalities. This invention provides a precise target and a novel strategy for the modification therapy of calcific aortic valve disease, solving the technical problem of the ineffectiveness of traditional broad-spectrum anti-inflammatory therapy in valvular calcification.

The invention discloses application of H-151 in preparation of drugs for preventing and treating calcified aortic valve diseases, relates to the technical field of biological medicines, and is characterized in that the invention provides application of an STING inhibitor H-151 or pharmaceutically acceptable salts thereof in preparation of drugs for preventing and / or treating calcified aortic valve diseases. The invention reveals for the first time that H-151 can inhibit the increase of the expression level of osteogenic differentiation markers ALP and RUNX2 in aortic valve interstitial cells induced by an osteogenic culture medium in an in-vitro experiment, and inhibit osteogenic differentiation and calcium salt deposition of the aortic valve interstitial cells; in addition, aortic valve thickening, fibrosis and calcium salt deposition of mice induced by an aortic valve injury model are inhibited in vivo. Based on the biological effect, the application of H-151 in developing the medicine for treating the calcified aortic valve disease is provided for the first time, and a new technical path and a new medicine choice are provided for preventing and treating the calcified aortic valve disease.

The invention relates to a fixing device for loading low-intensity pulsed ultrasound by taking water as a medium and application of the fixing device in promoting osteogenic differentiation of mesenchymal stem cells. According to the device, a double-base type probe fixing structure consisting of a main body fixing base and an accessory fixing base is arranged in a main body structure; and a culture vessel fixing structure on the inner wall of the main body structure is matched, so that the problems that a probe is easy to shift, the heights of culture vessels are inconsistent and the like in the prior art are effectively solved, and a standardized and high-repeatability loading environment is provided for an LIPUS in-vitro experiment. The device is further based on the device, water is used as an ultrasonic conduction medium to be applied to osteogenic differentiation induction of bone marrow mesenchymal stem cells (BMSCs), the efficient and stable osteogenic stimulation effect can be achieved, and the core defects that in the prior art, a probe makes direct contact with a culture vessel, consequently, cells fall off and are curled, morphological damage is caused, and sound field distribution is uneven are overcome; and meanwhile, the stability and repeatability of an experimental result are remarkably improved.

The invention belongs to the technical field of cell culture, and particularly relates to a bone marrow mesenchymal stem cell culture medium and a preparation method and application thereof. The mesenchymal stem cell culture medium comprises a basic culture medium, and betaine, glycyrrhizin, insulin, EGF (Epidermal Growth Factor), L-propyl dipeptide and naloxicol which are added into the basic culture medium. The bone marrow mesenchymal stem cell culture medium can effectively improve the multiplication capacity of bone marrow mesenchymal stem cells, delay cell senescence and improve the osteogenic differentiation capacity of the bone marrow mesenchymal stem cells, fetal calf serum is not added, the risk of introducing heterologous serum to carry viruses is avoided, and the safety of clinical application is improved.

The application relates to application of a medicine taking ACLY gene as an action target in preparation of a medicine for preventing and treating vascular calcification, and belongs to the technical field of biological medicine. In order to solve the problem that effective clinical means for treating vascular calcification is lacked at present, the application provides application of a medicine taking ACLY gene as an action target in preparation of a medicine for preventing and treating vascular calcification, the transcription or translation of the ACLY gene of vascular smooth muscle cells is efficiently and specifically inhibited, or the expression or activity of the ACLY protein of the vascular smooth muscle cells is efficiently and specifically reduced, the osteogenic differentiation of smooth muscle is reduced, the deposition of hydroxyapatite in the vascular media is reduced, and the progress of vascular calcification is alleviated. The medicine for preventing and treating vascular calcification is prepared on the basis of inhibition of the ACLY of vascular smooth muscle, has important clinical application potential for vascular calcification treatment, and can provide a new treatment option for patients with vascular calcification.

The invention discloses an application of loperamide in preparation of a medicine for treating osteoporosis, and belongs to the field of development of new application of medicines. Loperamide has the effects of inhibiting aging, increasing osteogenic differentiation of bone marrow mesenchymal stem cells and preventing osteoporosis of aged and postmenopausal mice; meanwhile, at the cellular level, loperamide can reduce expression of senescence factors in mouse mesenchymal stem cells, promote senescence cell apoptosis and inhibit cell senescence; and a new choice is provided for the treatment of senescence-related osteoporosis drugs.

The application provides an application of isoxazole 9 (ISX-9) in promoting mesenchymal stem cell osteogenic differentiation and bone injury regeneration repair. ISX-9 is a small molecule compound, and has the characteristics of easy to obtain, controllable structure and low cost. The in vitro experiment of the application shows that ISX-9 has the ability to induce mesenchymal stem cell osteogenic differentiation and promote the formation of mineralized nodules; the in vivo experiment shows that the hydrogel wrapped with ISX-9 can promote the regeneration repair of mouse skull defects, indicating that ISX-9 has good osteogenic induction and is a potential bone regeneration repair drug. Therefore, ISX-9 can promote mesenchymal stem cell osteogenic differentiation and be applied to bone tissue regeneration repair. The application also provides a direct action target and action mechanism of ISX-9 in promoting osteogenic differentiation. The application further provides a new drug sustained release delivery system of ISX-9, which is helpful for the application from theoretical research to clinical application.

The invention relates to the technical field of biological medicines, in particular to a galangal-sourced nano-vesicle as well as a preparation method and application thereof. The method specifically comprises the following steps: crushing rhizoma alpiniae officinarum rhizomes, mixing the crushed rhizoma alpiniae officinarum rhizomes with a buffer solution, soaking in a water bath, and filtering to remove solids so as to obtain a crude extract; and sequentially carrying out centrifugal treatment on the crude extract for four times, so as to obtain the galangal-derived nano-vesicles. The invention provides a method for extracting nano-vesicles from galangal, and finds for the first time that the obtained vesicles can promote osteogenic differentiation of human bone marrow mesenchymal stem cells with high efficiency and low toxicity, the effect of the vesicles is obviously superior to that of known active component galangin, and the cytotoxicity is obviously lower; the invention provides raw materials and methods with great potential for developing novel and safe anti-osteoporosis drugs or inducers.

The invention provides an embryonic stem cell differentiation technology and application of the embryonic stem cell differentiation technology in screening of unknown risk alternative toxicity of a novel biological breeding product, and Cry1Ab protein is used as a test substance to evaluate the developmental toxicity of the Cry1Ab protein. The EBs are generated through hanging drop culture, and bone differentiation induction substances (beta-glycerophosphate, ascorbic acid and vitamin D3) are added to promote differentiation of the EBs. After bone cell induced differentiation is finished, the differentiation condition of bone cells is observed through alizarin red S dyeing and absorbance value detection, the cell growth condition is observed through cell total protein concentration and alkaline phosphatase activity detection, and then the influence of a test substance on the bone differentiation process is analyzed through the gene expression condition of osteogenic differentiation related markers (Runx2, SPARC and I-type collagen). The invention solves the problem of evaluating the unknown risk of a novel biological breeding product in vitro, creates an embryonic stem cell bone differentiation test technology, and uses the embryonic stem cell bone differentiation test technology as a screening method for the unknown risk alternative toxicity of the novel biological breeding product to be combined with an embryonic stem cell myocardial differentiation experiment for use. And the accuracy of predicating the developmental toxicity of the test substance is improved.

The present invention relates to a composition for promoting the osteogenic differentiation efficiency of adipose-derived stem cells. The present invention has revealed that treating Adipose-Derived Stem Cells (ADSCs) from older donors with reduced differentiation efficiency with FGF2 and / or HGF during the early stages of differentiation can restore damaged paracrine function and maximize differentiation efficiency. Therefore, it can be utilized as an in vitro process technology for promoting stem cell differentiation efficiency before or during adipose-derived stem cell transplantation.

The invention discloses a long-chain non-coding RNA (Ribonucleic Acid) and application thereof. According to the invention, by analyzing and comparing the expression conditions of the long-chain non-coding RNA in a wild type mouse and an ER beta gene knockout mouse, the long-chain non-coding RNA participating in the regulation and control of the osteogenic differentiation of the bone marrow mesenchymal stem cells, namely the long-chain non-coding RNA MSTRG.47495.1 (lncRNA MSTRG.47495.1), is found. Overexpression of the long-chain non-coding RNA in the bone marrow mesenchymal stem cells can effectively promote osteogenic differentiation of the bone marrow mesenchymal stem cells, enhance bone regeneration and promote bone defect repair of rats with skull injury. On the basis, the long-chain non-coding RNA can promote osteogenic differentiation of bone marrow mesenchymal stem cells and enhance bone regeneration, and is used for preparing medicines for treating bone metabolic diseases and bone formation disorder related diseases. In addition, the long-chain non-coding RNA can also be used for construction of bone organs.

The application discloses application of ac4C modification of NAT10 and PCSK9 mRNA in diagnosis and treatment of DAVD, and belongs to the technical field of biological medicine. The application finds that NAT10 increases expression of PCSK9 by ac4C modification of PCSK9 mRNA, thereby promoting occurrence and development of degenerative aortic valve disease; by inhibiting expression of NAT10 to reduce the ac4C modification level of PCSK9 mRNA, osteogenic differentiation of hVICs can be effectively inhibited, and valve calcification can be delayed or inhibited. Therefore, the application proposes to jointly detect the ac4C modification levels of NAT10 and PCSK9 mRNA, which can be used for diagnosis of DAVD; and the ac4C modification of NAT10 and PCSK9 mRNA can be jointly targeted to prepare or screen drugs for treating DAVD. The application provides a new action target for precise diagnosis of DAVD and development of a targeted drug.

The invention discloses an osteogenic differentiation induction medium based on pilose antler polypeptide and an osteogenic differentiation induction method, and belongs to the technical field of biology. The osteogenic differentiation induction culture medium based on the cornu cervi pantotrichum polypeptide contains a cornu cervi pantotrichum polypeptide solution, dexamethasone, L-ascorbic acid, beta-sodium glycerophosphate and a complete culture medium, the cornu cervi pantotrichum polypeptide provided by the invention can greatly improve the osteogenic differentiation effect of mesenchymal stem cells as an additive component of an osteogenic induction culture medium, and is superior to a commercial osteogenic induction culture medium in a certain scene; according to the osteogenic induction differentiation method disclosed by the invention, the defects in the prior art are improved, and the osteogenic differentiation effect of the mesenchymal stem cells can be improved by adopting the pilose antler polypeptide osteogenic differentiation induction culture medium.

The application provides a bone nodule organoid and a preparation method and application thereof, the bone nodule organoid comprises a microsphere carrier system, the microsphere carrier system is made of CGRP, BMP-2, bone marrow mesenchymal stem cells and GelMA-HA-NB hydrogel by a light curing 3D printing technology, the microsphere carrier system is mixed with GelMA hydrogel containing SP to be crosslinked and solidified to form the bone nodule organoid, and the bone nodule organoid is used for in-vivo transplantation to stimulate endogenous bone tissue rapid regeneration;The bone nodule organoid has the ability of two-phase delivery of bioactive factors, different bioactive factors can realize time sequence, differential low dose, slow and continuous release, so that the healing speed and bone repair effect of large-size bone defect are accelerated in a divide-and-rule manner.The application also provides a bioactive factor composition of a bionic bone nodule tissue neurotrophic factor and a physiological dose of bone morphogenetic protein, the composition releases the bone marrow mesenchymal stem cell osteogenic differentiation in the bone nodule organoid according to signal time sequence.

This invention discloses a near-infrared fluorescent probe for detecting bone regeneration vitality and its preparation method, belonging to the field of biochemistry. Using rhodamine as the fluorescent core, the invention utilizes quinoline ring expansion to increase the conjugated area and extend emission. The brightness and stability of the probe are controlled by adjusting the steric hindrance of the aromatic ring at position 9, constructing a series of stable, high-brightness probe cores with low pKa. By introducing a phosphate ester group as a responder while simultaneously quenching the fluorescence of the core, a near-infrared fluorescent probe, SiRd-MOP, for detecting bone regeneration vitality is obtained. The preparation method of this invention has high yield and mild reaction conditions. The prepared probe can specifically and sensitively respond to alkaline phosphatase (ALP), and has successfully achieved ALP activity monitoring in osteogenic differentiation models and throughout the entire process of fracture regeneration and repair in mice. It can be used for real-time assessment of bone regeneration vitality and has very high biological application prospects.

The invention relates to the technical field of biology, in particular to novel application of sanguinarine (SAN), a method for inducing human amniotic mesenchymal stem cells (hAMSCs) to be differentiated into osteoblasts through COX2 protein and an osteogenic induction culture medium for the inducing method, and the method can be applied to the fields of bone disease treatment drug research and development and clinical transformation. It is proved for the first time that SAN mediates hAMSCs osteogenic differentiation through COX2 protein, SAN can significantly improve expression of COX2 gene (PTGS2) in hAMSCs, the osteogenic effect of SAN can be reversed by adding a COX2 inhibitor (such as celecoxib), and direct experimental evidence is provided for'COX2 serving as a small molecule drug to promote osteogenic differentiation '.

The application discloses a 4D printing shape memory composite stent for delivering exosomes and magnesium ions and a preparation method and application thereof. The preparation method comprises the following steps: (1) mixing hydroxyapatite and silicon dioxide nanoparticles with poly(D, L-lactic acid-co-trimethylene carbonate) in dichloromethane to obtain an oil phase; adding exosomes into a magnesium oxide nanoparticle water dispersion to obtain an aqueous phase; and mixing to obtain a water-in-oil Pickering emulsion; and (2) using a 4D printing technology to prepare the composite porous shape memory stent (PHS / MgO-EXO) loaded with exosomes by using the Pickering emulsion ink. The stent has a multi-stage interconnected pore structure, and can promote cell osteogenic differentiation and bone tissue regeneration by synergistically releasing exosomes and magnesium ions. The stent also has a temperature-sensitive shape memory function, can restore the original shape under a slightly higher body temperature stimulus, can accurately match irregular bone defects, and is suitable for minimally invasive repair treatment of clinical irregular bone defects.

The application belongs to the technical field of medicine, and particularly relates to a use of a flavonoid compound in aortic valve disease or cardiovascular aging. The flavonoid compound shown in formula I is found to be capable of inhibiting the osteogenic differentiation of valve interstitial cells for the first time. In animal implementation, the flavonoid compound can significantly reduce the peak flow rate and peak pressure difference of the aortic valve of a calcified aortic valve mouse, and has a significant effect of relieving aortic valve calcification. Therefore, the flavonoid compound has a good application prospect in the preparation of a drug for relieving aortic valve disease, relieving cardiovascular aging and other related cardiovascular diseases.

The invention discloses application of oroxin B in preparation of a medicine for promoting osteogenic differentiation of bone marrow mesenchymal stem cells, hydrogel of the oroxin B and a preparation method of the hydrogel, and belongs to the technical field of biomedical materials. The invention firstly provides application of oroxin B in preparation of a medicine for promoting osteogenic differentiation of bone marrow mesenchymal stem cells. The invention also provides a self-assembled polypeptide hydrogel loaded with the oroxin B. The hydrogel is obtained by combining the oroxin B with the self-assembled polypeptide with a specific sequence (SEQ ID No.1) and carrying out mild physical blending and incubation. The hydrogel forms a three-dimensional nanofiber network, can entrap and continuously release oroxin B in situ, not only can effectively collect bone marrow mesenchymal stem cells, but also can provide an ideal microenvironment for proliferation and osteogenic differentiation of the bone marrow mesenchymal stem cells, and remarkably up-regulate osteogenic related gene expression, so that bone defect repair is expected to be accelerated.

The invention discloses a near-infrared fluorescent probe for detecting bone regeneration activity and a preparation method of the near-infrared fluorescent probe, and relates to the technical field of biochemistry. According to the invention, rhodamine is taken as a fluorescent parent nucleus, quinoline ring expansion is utilized to enlarge the conjugate area and prolong emission, and the brightness and stability of the probe are regulated by regulating the steric hindrance of a ninth aromatic ring, so that a series of stable and high-brightness probe parent nucleuses with smaller pKa are constructed. The near-infrared fluorescent probe SiRd-MOP for detecting the bone regeneration activity is obtained by introducing a phosphate ester group as a response group and quenching parent nucleus fluorescence at the same time. The preparation method is high in yield and mild in reaction condition, the prepared probe can specifically and sensitively respond to alkaline phosphatase (ALP), ALP activity monitoring in the whole process of fracture regeneration and repair of a cell osteogenic differentiation model and a mouse is successfully achieved, the probe can be used for evaluating bone regeneration activity in real time, and the probe has very high biological application prospects.

This invention provides an application of the PDK4 gene, which is used to prepare drugs to improve postmenopausal osteoporosis. PDK4 gene deletion in bone marrow mesenchymal stem cells (BMSCs) reduces their osteogenic differentiation capacity and inhibits mitochondrial division by blocking the E2-DRP1 signaling axis. Overexpression in bone tissue reverses bone formation inhibition caused by postmenopausal osteoporosis. This invention redefines the biological function of the PDK4 gene in the skeletal system. This gene promotes osteogenic differentiation of BMSCs through the E2-ERα-PDK4-MYH9-DRP1 signaling axis, thus improving postmenopausal osteoporosis and providing a new theoretical framework and potential target for developing bone-promoting therapies for postmenopausal osteoporosis.

The invention provides a preparation method of an anti-inflammatory bone repair material for promoting angiogenesis, which comprises the following steps: adding amorphous calcium magnesium phosphate powder into a polylactic acid solution, carrying out ultrasonic treatment to uniformly disperse the amorphous calcium magnesium phosphate powder, and carrying out vacuum freeze drying to obtain the anti-inflammatory bone repair material. The mass ratio of the amorphous calcium magnesium phosphate powder to the polylactic acid is (1: 9)-(1: 1). Amorphous calcium magnesium phosphate is added into polylactic acid, so that a large amount of calcium magnesium phosphorus can be released after implantation, bone defect stem cell osteogenic differentiation and angiogenesis are promoted, and the biocompatibility of polylactic acid is improved. The addition of inorganic matters can accelerate the degradation rate of polylactic acid, and prevent the bone repair material from occupying for a long time to affect the generation of new bones. Meanwhile, the amorphous calcium magnesium phosphate can neutralize an acid environment generated by degradation of polylactic acid, and the inflammatory response of the defect part is relieved.

The present application relates to a kind of drug based on PARM1 regulation treatment calcified aortic valve disease and its application.The present application first finds that PARM1 is significantly increased in the aortic valve of calcified aortic valve disease (CAVD) patient, and plays a key role in the osteogenic differentiation process of valve interstitial cells (VICs), and the expression level of PARM1 is knocked down can inhibit the osteogenic differentiation of VICs and extracellular calcium salt deposition, reduce aortic valve calcification, and its mechanism of action involves MAPK signal pathway.The present application provides a new target and treatment idea for the treatment of CAVD, and can be applied to related drug research and development and treatment method development.