Verification method for stimulating osteogenic differentiation of valvular interstitial cells by inorganic phosphate

An inorganic phosphate, osteogenic differentiation technology, applied in biochemical equipment and methods, microbial determination/inspection, analysis of materials, etc., can solve the problems of long modeling time and low efficiency

Active Publication Date: 2020-03-31
THE SECOND AFFILIATED HOSPITAL TO NANCHANG UNIV
View PDF7 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the process of constructing the classic in vitro calcification model using classic organophosphates such as sodium β-glycerophosphate, there are long modeling time (14-21 days) and low efficiency Although some scholars have pointed out t

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Verification method for stimulating osteogenic differentiation of valvular interstitial cells by inorganic phosphate
  • Verification method for stimulating osteogenic differentiation of valvular interstitial cells by inorganic phosphate
  • Verification method for stimulating osteogenic differentiation of valvular interstitial cells by inorganic phosphate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0087] In order to facilitate the understanding of the present invention, the following will describe the present invention more fully. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that the disclosure of the present invention will be thorough and complete.

[0088] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and / or" includes any and all combinations of one or more of the associated listed items.

[0089] It should be noted that, in the present invention, DMEM (ulbecco's modified eagle medium) medium (containing 4.5g / L d...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a verification method for stimulating osteogenic differentiation of valvular interstitial cells by inorganic phosphate. The method comprises the following steps: respectivelyperforming osteogenic differentiation culturing on extracted rat valvular interstitial cells by using CON, IP-OIM and OIM and then extracting protein to prepare a protein sample; after ALP staining and alizarin red staining on three kinds of protein samples, evaluating an osteogenic differentiation degree after staining by using ALP activity detection and a relative calcium content determination method, and detecting expression changes of BMP-2, RhoA and Rock-1 through a WB method and an RT-PCR method respectively; with the si-RNA technology, respectively interfering the expression of BMP-2 and RhoA and observing the relationship of mutual changes between the BMP-2 and the RhoA and whether the BMP-2 and the RhoA have the effect of resisting osteogenic differentiation or not after interference; and studying the influence of IP-OIM on SMAD1/5/9 phosphorylation as well as a mutual relation between two paths of BMP-2/SMAD1/5/9 and RhoA/Rock-1. According to the invention, facts that calcification formation of valvular interstitial cells is caused by inorganic phosphate through BMP-2/smald1/5/9 and RhoA/Rock-1 signal pathways and the BMP-2/SMAD1/5/9 pathway is an upstream pathway of theRhoA/Rock-1 pathway in the valvular interstitial cell osteogenic differentiation process are determined; and a forward regulation effect is realized.

Description

technical field [0001] The invention relates to the technical field of osteogenic differentiation of valve interstitial cells, in particular to a method for verifying that inorganic phosphate stimulates the osteogenic differentiation of valve interstitial cells. Background technique [0002] Calcific aortic valve disease refers to the loss of elasticity of the valve leaflets due to valve degeneration or calcium salt deposition, resulting in varying degrees of left ventricular outflow tract stenosis. Valve interstitial cells (valvular interstitial cells, VICs) play a role similar to the cytoskeleton in the valve to maintain the shape of the valve leaflets. Under certain conditions, it can also differentiate into the direction of osteogenic phenotype. Valve interstitial cells are also considered as the main cells in the process of aortic valve calcification, and from the pathological characteristics of calcified valves, they have bone tissue-like features. [0003] In relate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/02
CPCG01N33/5041C12N2503/00
Inventor 周建良芦潮余文朋聂彬恩丁静丽林峰杨威董啸龚艺刘季春徐建军唐燕华
Owner THE SECOND AFFILIATED HOSPITAL TO NANCHANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap