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Tomato S1CYP90B3 gene and application thereof

A gene and tomato technology, applied in the application field of tomato SlCYP90B3 gene in improving tomato fruit resistance to chilling damage, can solve problems such as waste, and achieve the effect of improving resistance and good resistance to chilling damage

Active Publication Date: 2020-03-20
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

lead to serious waste and economic loss

Method used

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  • Tomato S1CYP90B3 gene and application thereof
  • Tomato S1CYP90B3 gene and application thereof
  • Tomato S1CYP90B3 gene and application thereof

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Embodiment Construction

[0021] The present invention is further described below in conjunction with specific embodiment, but protection scope of the present invention is not limited thereto:

[0022] 1. Obtain the tomato SlCYP90B3 gene and the construction of the gene overexpression vector:

[0023] Primer Premier 5.0 was used to design bidirectional amplification primers for the gene, and the leaves of wild-type tomato AC (Ailsa Craig) planted in the Huajiachi greenhouse of Zhejiang University were taken, and the cDNA obtained by extracting RNA and reverse transcription was used as a template (the extraction method of cDNA was a conventional method, Refer to patent CN104561025A), the upstream specific primer is SlCYP90B3-F and the downstream primer is SlCYP90B3-R, and the full-length SlCYP90B3 gene (SEQ ID No: 1) is amplified with PrimerSTAR high-fidelity enzyme.

[0024] The primer sequence is: SlCYP90B3-F: 5'-ATGTCTGACTTAGAGTTTTTTCTTTTTCTTATTCC-3'

[0025] SlCYP90B3-R: 5'-GTCATGTAATTTTATTTAACGATC...

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Abstract

The invention discloses a gene S1CYP90B3 for improving cold-damage resistance of tomato fruits. A nucleotide sequence of the gene is shown in SEQ ID No:1. The invention also discloses an application of the gene in improving cold-damage resistance of tomato fruits, the gene is used for constructing transgenic tomato, and the transgenic tomato can improve cold-damage resistance of fruits.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of tomato SlCYP90B3 gene in improving the resistance of tomato fruit to cold damage. Background technique [0002] Refrigeration is one of the most common means of maintaining the quality of vegetables and fruits after harvest. However, vegetables with low-temperature-sensitive fruits are prone to chilling injury under low-temperature storage conditions. Tomato is a typical model plant for studying the occurrence of fruit chilling injury. Tomato fruits with chilling damage exhibit various symptoms, including fruit surface damage, disease, and inability to fully turn red. These changes reduce fruit quality and consumer acceptance. Lead to serious waste and economic loss. [0003] Various strategies have been employed over the years to reduce the incidence of chilling injury in tomato fruit, including chemical treatments or physical methods such as methyl jasmonate, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N9/02C12N1/21C12N15/84A01H5/08A01H6/82C12R1/19C12R1/01
CPCC12N9/0071C12N15/8273C12N15/8298
Inventor 汪俏梅胡松申刘丽红李硕邵志勇孟凡亮刘浩然梁冬怡
Owner ZHEJIANG UNIV
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