21 results about "Genetically modified tomato" patented technology

The invention belongs to the technical field of crop breeding by a gene editing technology, and particularly relates to a method for creating a novel tomato cytoplasmic male sterility germplasm through gene editing and an application of the novel tomato cytoplasmic male sterility germplasm. In order to solve the problems that existing cytoplasmic male sterile materials in tomatoes are insufficient, production of tomato hybrid seeds is restricted and the like, gene editing is performed on MSH1 through a CRISPR / Cas9 technology, cytoplasmic male sterile single plants are identified in mutant selfing separation progenies and hybridized with wild types twice, and the tomato hybrid seeds are obtained. Finally, a novel non-transgenic tomato cytoplasmic male sterility material with the MSH1 gene recovered to a wild type is obtained, and meanwhile, the horticultural character of the original fertile material is kept unchanged.

The application discloses a red raspberry RuSA1 gene, an expression protein thereof and application, and relates to the technical field of plant genetic engineering.The red raspberry RuSA1 gene disclosed by the application has a nucleotide sequence as shown in SEQ ID NO.1, and the amino acid sequence of the expression protein thereof is as shown in SEQ ID NO.2.The application constructs an expression vector of the red raspberry RuSA1 gene, and then transforms the expression vector into tomatoes;transgenic tomato plants with significantly increased anthocyanin content are obtained through cultivation and screening.The RuSA1 gene promotes the synthesis of anthocyanins in the fruits of the transgenic lines.The expression amounts of the genes SlABA, SlNCED1, SlACS2 and SlACO1 related to the secretion of ABA hormone, the gene SlIAA36 related to the secretion of IAA hormone, and the genes Sl2ox4 and Sl20x2 related to the secretion of GA hormone are all significantly higher than those of CK.

The invention discloses an SlFMO1 gene and application thereof in regulation and control of growth and development of tomato plants, and belongs to the technical field of gene engineering. According to the method, a CRISPR / Cas9 gene editing technology is utilized, firstly, a CRISPR / Cas9-gRNA vector is constructed to accurately edit an SlFMO1 gene in tomatoes, GV3101 agrobacterium tumefaciens is utilized to conduct mediated transformation on the tomatoes, and kanamycin serves as a resistance marker to conduct screening to obtain SlFMO1-deleted mutant tomatoes. Phenotype observation and analysis show that after the SlFMO1 gene is knocked out, the plant height and the plant width of a tomato plant are obviously increased, the apical development is enhanced, the stem diameter of the plant is increased, and the inflorescence spacing is increased, which indicates that the SlFMO1 gene negatively regulates the growth and development of the tomato, and meanwhile, the expression of related genes synthesized by auxin, gibberellin and brassinolide is obviously enhanced. The discovery has important theoretical significance and practical application value for follow-up cultivation of ideal tomato varieties.

The invention discloses a preparation method and application of ackermania mucophilia outer membrane protein transgenic tomatoes. The preparation method provided by the invention comprises the following steps: transforming a codon-optimized ackermann mucophile outer membrane protein gene into competent cells through a cloning vector, and infecting a cultured tomato explant by adopting the transformed competent cells to obtain the ackermann mucophile outer membrane protein. And culturing the transgenic tomato expressing the ackermann mucophile outer membrane protein by adopting the infected explant. According to the preparation method, the AMUC1100 protein can be expressed in tomatoes, the tomato fruits are used for preventing or treating the metabolic dysfunction related fatty liver diseases, the treatment effect of the AMUC1100 protein can be obtained, the functions of the tomatoes can be coordinated, and the effect of preventing or treating the metabolic dysfunction related fatty liver diseases is achieved. A new scheme and strategy are provided for prevention or treatment of metabolic dysfunction related fatty liver diseases; in addition, the problems of infection risk in viable bacteria treatment, high viable bacteria storage and transportation cost and the like are solved.

The invention belongs to the technical field of biology, and relates to a method for improving a tomato fruit shape index and related genes and proteins. The shape of the tomato fruit is changed by changing the cell expansion direction of the tomato fruit. Experiments prove that SlbHLH113 overexpressed transgenic tomato fruit cells are more inclined to extend along the longitudinal axis than wild tomato fruit cells, so that the longitudinal diameter of the fruits is increased, the transverse diameter is reduced, and the fruit shape index is remarkably improved. Therefore, the SlbHLH113 gene is related to the shape of the tomato fruit; the SlbHLH113 protein and part of coding genes thereof can be used for cultivating new tomato varieties with elongated fruit shapes, and play an important role in the field of plant variety breeding.

The invention belongs to the technical field of biology, and particularly relates to application of a tomato SlCYP90B3 gene in regulation and control of tomato fruit color, and the nucleotide sequence of the SlCYP90B3 gene is shown as SEQ ID No: 1. According to the invention, the tomato SlCYP90B3 gene is silenced, so that the tomato with higher flavonoid content and richer color is cultivated. The transgenic tomato obtained by the method disclosed by the invention can improve the content of flavonoids in the tomato peel so as to influence the color of the tomato.

The invention discloses application of a VcBR6OX1 gene in seedless fruit formation, and belongs to the technical field of molecular biology. According to the invention, the BR6OX1 gene in blueberry is transferred into tomato through a genetic transformation technology, and phenotype identification and physiological and biochemical index determination on transgenic tomato prove that overexpression of VcBR6OX1 can promote formation of seedless fruits. Through genetic transformation and functional analysis, the action mechanism of BR6OX1 in fruit development is disclosed. Meanwhile, transcriptome analysis is carried out on transgenic tomatoes and wild tomatoes, downstream genes responding to BR6OX1 are found, a theoretical basis is provided for development of seedless fruits, and a foundation is laid for application of the genes in seedless fruit breeding.

The invention provides an eukaryotic recombinant plasmid and application thereof, relates to the field of plant genetic engineering, and discloses an eukaryotic recombinant plasmid, the eukaryotic recombinant plasmid is an SlTCP12 constitutive CRISPR / Cas9 gene knockout vector, the SlTCP12 constitutive CRISPR / Cas9 gene knockout vector is formed by connecting a TCP12 gene knockout target spot and a plant gene editing vector pKSE401, and the TCP12 gene knockout target spot is connected with the plant gene editing vector pKSE401. The invention further discloses a preparation method and application. The invention provides a novel eukaryotic recombinant plasmid for improving the resistance of tomatoes to pseudomonas syringae, and the eukaryotic recombinant plasmid can reduce tomato leaf scab, reduce the number of infected colonies and increase the expression quantity of immune related genes, and can enhance the antibacterial ability of plants, thereby enhancing the immune response of tomato plants to pathogens of the bacteria. The gene used in the invention is the gene of the tomato, and the finally obtained transgenic tomato has high safety performance.

The application provides application of SlPRR5 gene in regulation of chlorophyll synthesis and photosynthetic efficiency of tomato. The nucleotide sequence of the SlPRR5 gene is shown as SEQ ID NO. 1. The application constructs transgenic tomato plants of overexpression and gene knockout of the SlPRR5 gene, and finds that the SlPRR5 gene can effectively regulate the synthesis of chlorophyll of the tomato, significantly regulate the photosynthetic efficiency of the tomato plant, and change the growth state of the plant by observing the chlorophyll content and photosynthetic capacity of the tomato. The application provides a new gene regulation strategy for optimization of photosynthetic capacity and yield improvement of the tomato.

The present invention relates to a transgenic tomato plant, a production method therefor, a seed thereof, and a vitamin E-enriched food composition comprising same, wherein the transgenic tomato plant has a methyl-pylbenzoquinol methyltransferase (MPBQMT)-encoding gene introduced thereinto and exhibits a significantly increased content of alpha-tocopherol in a fruit compared to a wild type. The transgenic tomato of the present invention exhibits an increase in α-tocopherol content of up to 3.4 times or more compared to the wild type by promoting vitamin E biosynthesis through overexpression of the MPBQMT gene, and can be usefully applied to the development of high value-added tomato varieties with enhanced functionality.

The present disclosure relates to transgenic tomato plants, which have been transformed to produce a fragment of respiratory syncytial virus (RSV) F protein, and the plant-optimized plasmids that produce such transgenic tomato plants. In some aspects, the transgenic tomato plant expresses the ectodomain of RSV F protein. In certain embodiments, the fragment of RSV F protein comprises the ectodomain of RSV F protein, and the p27 region of the RSV F protein is replaced with a sequence-specific linker region comprising the amino acid sequence of GSGSGRSLG. Also described are compositions comprising an extraction from a fruit of the transgenic tomato plant, wherein the extraction from the fruit of the transgenic tomato plant comprises the fragment of RSV F protein.

This invention discloses SlBPC1 The application of genes in regulating tomato lateral branch development belongs to the field of biotechnology. This invention successfully constructed... SlBPC1 Transgenic tomato plants with gene overexpression and CRISPR / Cas9 gene knockout were confirmed through phenotypic observation and statistical data analysis to have the following effects: SlBPC1 This invention can significantly increase the number of lateral branches in tomatoes and promote their elongation, while knocking out this gene can effectively inhibit the number and growth of lateral branches, thereby creating a compact, rational, and suitable ideal plant shape. This invention clarifies... SlBPC1 The biological functions of genes in regulating the development of lateral branches in tomatoes can enable targeted improvement of tomato plant architecture, providing important genetic resources and theoretical basis for optimizing tomato plant architecture and breeding new varieties.

The application discloses a banana burrowing nematode branch acid mutase and application thereof. The application provides application of a banana burrowing nematode branch acid mutase gene RsCM in prevention and treatment of banana burrowing nematodes, and the nucleotide sequence of the banana burrowing nematode branch acid mutase gene RsCM is shown in SEQ ID NO. 1 or SEQ ID NO. 2. Tobacco transient expression shows that RsCM can significantly inhibit flg22-induced defense gene expression and callose deposition, and can significantly inhibit BAX-induced cell necrosis, which indicates that RsCM can inhibit the defense response of plants; a transgenic tomato is constructed through in planta RNAi technology, and it is found that the pathogenicity of banana burrowing nematodes is reduced after inoculation of the nematodes. The application provides a new strategy for prevention and treatment of nematodes in agricultural production, provides a new target for cultivation of nematode-resistant plant varieties, and provides a new approach for prevention and treatment of banana burrowing nematodes.

The invention discloses application of a pomegranate PgXTH5 gene in reduction of fruit cracking rate, and belongs to the technical field of plant genetic engineering and molecular breeding. According to the application of the pomegranate PgXTH5 gene to reduction of the fruit cracking rate, protein coded by the pomegranate PgXTH5 gene is over-expressed in a plant, the nucleotide sequence of the pomegranate PgXTH5 gene is shown as SEQ ID NO.1, and the amino acid sequence of the protein coded by the pomegranate PgXTH5 gene is shown as SEQ ID NO.2. Experiments show that through overexpression of the PgXTH5 gene in transgenic tomatoes, the toughness of peel cell walls can be effectively enhanced, and the fruit cracking rate is remarkably reduced compared with that of wild tomatoes. Therefore, the application provided by the invention can effectively improve the yield and economic benefits of plant fruits, and has a good application prospect.

The invention relates to the production of a drought-resistant genetically modified tomato by silencing Solyc01g080540 with SEQ ID NO:9 sequence and Solyc06084410 with SEQ ID NO:12 sequence from the AHP family involved in cytokinin signalling in tomato (Solarium lycopersicum) by the CRISPR / Cas9 gene editing method. In the said CRISPR / Cas9 system, gRNA 3 with nucleotide sequence SEQ ID NO:1, gRNA 25 with nucleotide sequence SEQ ID NO:2, gRNA 29 with nucleotide sequence SEQ ID NO:3 and gRNA 78 with nucleotide sequence SEQ ID NO:4 are used to target Solyc01g080540 and Solyc06084410 genes and to create mutations. The mutant tomato lines subject to the invention show a drought-resistant phenotype with physiological characteristics such as decreased stomatal number, increased root length and lateral root number.

The invention discloses application of an E8 promoter and an RH1 gene to creation of an anthocyanin-rich tomato strain, and belongs to the technical field of tomato strain cultivation. The nucleotide sequence of the E8 promoter is as shown in SEQ ID No.1, the nucleotide sequence of the RH1 gene is as shown in SEQ ID No.2, and the CDS nucleotide sequence of the RH1 gene is as shown in SEQ ID No.3. The E8 promoter and the CDS of the RH1 gene are combined to construct an expression vector pCAMBIA2305-E8-RH1-5xFLAG, the expression vector is introduced into cotyledons of tomato plants by utilizing an agrobacterium-mediated genetic transformation technology, the RH1 gene is driven by the E8 promoter to be highly expressed in tomato fruits, and the expression vector pCAMBIA2305-E8-RH1-5xFLAG is obtained. Inducing accumulation of anthocyanin in the fruits; tests show that the expression level of the RH1 gene in transgenic tomato fruits is remarkably improved, the anthocyanin content is close to 0.3 mg / gFW, and the anthocyanin-rich tomato strain is obtained.

The invention relates to an application of an SlCLE3 gene and polypeptide thereof in improvement of salt tolerance of tomatoes. Specifically, the nucleotide sequence of the SlCLE3 gene is as shown in SEQ ID NO: 1. According to the invention, an overexpression vector of the SlCLE3 gene is constructed, and an agrobacterium tumefaciens-mediated genetic transformation method is utilized to obtain a transgenic tomato homozygous strain overexpressed by the SlCLE3. Under the condition of salt stress, the transgenic line shows significantly enhanced salt tolerance, specifically, active oxygen accumulation is reduced, cell damage is reduced, photosynthetic indexes are improved, and relative conductivity is reduced. In addition, the damage of salt stress to tomato plants can be effectively relieved by exogenously applying the pCLE3 polypeptide. The invention provides a new gene resource and a breeding method for tomato salt-tolerant breeding.

The invention relates to the field of gene engineering, in particular to application of a waxberry MrDof5.4 gene in improvement of agronomic characters of tomatoes. According to the invention, a key gene MrDof5.4 which responds to a light signal and regulates and controls early development and quality formation of fruits is identified from waxberries through a specific low-intensity red laser processing model in combination with multi-omics analysis for the first time. Furthermore, by heterologous expression of the MrDof5.4 gene in a model crop tomato, it is verified that the MrDof5.4 gene can remarkably improve the plant height, stem diameter, single fruit weight and single plant yield of the transgenic tomato, and therefore the cross-species application potential of the gene in the aspects of improving the comprehensive agronomic traits of crops and improving the yield is verified.

The invention discloses a transgenic tomato with high expression of a human longevity gene as well as a preparation method and application of the transgenic tomato. The transgenic tomato is obtained by introducing human longevity genes PF4, SIRT1 and Klotho into a tomato genome through a genetic engineering technology, the longevity genes in the transgenic tomato are expressed in fruits of the transgenic tomato, and the method is that the human longevity genes are introduced into the tomato through an agrobacterium-mediated transformation method; the invention also discloses application of the transgenic tomato in food and medicine, and application of the transgenic tomato in promoting longevity, delaying senescence process, improving human intelligence and physical strength and enhancing immunity. Through high expression of human longevity genes, the level of human health related genes in fruits can be remarkably improved, so that intelligence, physical strength and life are improved; transgenic tomatoes with high expression of human longevity genes can generate corresponding proteins in fruits, and the proteins have positive influence on health of the fruits of the tomatoes, namely intelligence enhancement, physical strength enhancement and life prolonging.

The application discloses a blackberry RubHLH gene, an expression protein thereof and application, and relates to the technical field of plant genetic engineering.The blackberry RubHLH gene disclosed by the application has a nucleotide sequence as shown in SEQ ID NO.1, and the amino acid sequence of the expression protein thereof is as shown in SEQ ID NO.2.The application constructs an expression vector of the blackberry RubHLH gene, transforms the expression vector into tomatoes, and cultivates, screens and obtains transgenic tomato plants with significantly reduced anthocyanin substances.The RubHLH gene disclosed by the application inhibits the synthesis of anthocyanin substances such as pelargonidin, proanthocyanidin, naringenin, kaempferol-3-O-rutinoside, delphinidin-3-O-rhamnoside and petunidin-3-O-acetylglucoside.The leaf of the transgenic strain is larger, darker, wrinkled and has a shape significantly different from that of CK;the number of seeds of the fruit is reduced, and the fruit matures in advance.

The present invention discloses transgenic tomato microvesicle nanoparticles, a preparation method, and applications thereof, relating to the field of biotechnology. The preparation method comprises the following steps: (1) connecting a DNA molecule of lncENAF into a gene expression vector, which is then transformed into competent cells to obtain a recombinant microbial strain; (2) infecting a tomato explant with the recombinant microbial strain, followed by tissue culture and identification screening to obtain a transgenic tomato plant; (3) culturing the transgenic tomato plant to produce transgenic tomato fruit; and (4) extracting microvesicles from the transgenic tomato fruit to obtain microvesicle nanoparticles containing lncENAF. The present invention successfully achieves the genetic transformation of animal-derived lncENAF in plants. The transgenic tomato fruit prepared using the method of the present invention can be used to extract microvesicle nanoparticles containing lncENAF, which can be used to prepare drugs to relieve inflammation.