44 results about "Palindromic sequence" patented technology

Oligonucleotides containing the non-palindromic sequence motif:X1X2X3X4X5X6X7X8,wherein X1 is C,T,G or A (preferably T or C); wherein X2 is C,T,G or A; wherein X7 is C,T,G or A (preferably G); at least three, and preferably all, of X3, X4, X5, X6 and X8 are T; and with the proviso that, in the motif, a C does not precede a G (in other terms, the nucleic acid motif does not consist of a CpG oligonucleotide), that modulate the immune response of animals of the order Primate, including humans, are disclosed. This immune modulation is characterized by stimulation of proliferation, differentiation, cytokine production and antibody production on B-cells and cell differentiation on plasmacytoid dendritic cells.

A primer for the amplification of a DNA template comprising a protelomerase target sequence, particularly for production of closed linear DNA, which primer is capable of specifically binding to a palindromic sequence within a protelomerase target sequence and priming amplification in both directions.

A novel immunostimulatory oligonucleotide by which an IFN-inducing activity is enhanced and an inflammatory cytokine-inducing activity is reduced, and a pharmaceutical containing the same, and an application thereof are provided. That is, the present invention provides the immunostimulatory oligonucleotide composed of a base sequence represented by a formula: 5′-(G)MPXCGYQ(G)N-3′ (SEQ ID NO: 118) (X and Y are mutually independent and represent an arbitrary sequence which has a length of 0 to 10 nucleotides and does not contain 4 or more consecutive G residues, and a length of X+Y is 6 to 20 nucleotides; XCGY contains a palindrome sequence having a length of at least 8 nucleotides and has a length of 8 to 22 nucleotides; P and Q are mutually independent and represent one nucleotide other than G; M represents an integer of 6 to 10 and N represents an integer of 0 to 3) wherein a full length thereof is 16 to 37 nucleotides (except for an oligonucleotide composed of a base sequence represented by SEQ ID NO:5), the pharmaceutical application thereof.

The invention provides a method and a kit for constructing a sequencing library. The method comprises the following steps: performing enzyme digestion on a genome DNA of a sample to be tested to obtain an enzyme digestion fragment with sticky terminals; adding P1 connectors at the two ends of the enzyme digestion fragment to obtain a fragment with the P1 connectors; fragmenting the fragment with the P1 connectors to obtain fragments with the target sizes; adding P2 connectors at the two ends of the fragments with the target sizes to obtain a sequencing library, wherein the genome DNA comprises a recognition sequence which can be recognized by an enzyme; the recognition sequence comprises a palindromic sequence consisting of basic groups at the two ends of the recognition sequence, and a variable sequence in the middle of the palindromic sequence; the variable sequence comprises a one or more basic groups; the sticky terminals comprise one or more basic groups in the variable sequence. Through enzyme digestion on the enzyme of the variable sequence in the recognition sequence, different capturing tag numbers can be achieved through the types and the number of the basic groups in the fixing connectors, so that the flexibility is improved.

The invention relates to an asymmetrical hairpin probe and an application thereof, and belongs to the biological detection field. The asymmetrical hairpin probe consists of a target nucleotide distinguished sequence and a palindromic sequence; under the condition of no target sequence, the asymmetrical hairpin probe is under the metastable hairpin structure; once a target sequence to be detected is added, chain-type polymerization reaction free from the participation of enzyme is triggered; the target nucleic acid distinguished sequence part of the asymmetrical hairpin probe is combined with a corresponding target nucleic acid locus; rest probes are pairwise renatured into parts of double-chain probe of which the two ends are both provided with the palindromic sequence; and during molecular recognition hybridization, a detection signal is substantially enhanced so as to quickly detect the target sequence to be detected. According to the chain reaction technology of the asymmetrical hairpin probe, the high specificity of molecular hybridization and high efficiency of chain type polymerization are artfully combined, and free energy released by DNA (deoxyribose nucleic acid) polymerization is used for driving molecular cascade amplification. The asymmetrical hairpin probe has the advantages of simple reaction system and high detection efficiency, and the laboratory pollution problem existing in the traditional technology, such as PCR (polymerase chain reaction) and the like, can be effectively avoided.

Oligonucleotides containing the non-palindromic sequence motif: X1X2X3X4X5X6X7X8, wherein X1 is C,T,G or A (preferably T or C); wherein X2 is C,T,G or A; wherein X7 is C,T,G or A (preferably G); at least three, and preferably all, of X3, X4, X5, X6 and X8 are T; and with the proviso that, in the motif, a C does not precede a G (in other terms, the nucleic acid motif does not consist of a CpG oligonucleotide), that modulate the immune response of animals of the order Primate, including humans, are disclosed. This immune modulation is characterized by stimulation of proliferation, differentiation, cytokine production and antibody production on B-cells and cell differentiation on plasmacytoid dendritic cells.

The invention discloses an EMSA method, a probe thereof and a preparation method of the probe. The EMSA probe comprises a first strip chain, and the first strip chain comprises a transcription factor combination sequence and joint sequences located at the 5' end and the 3' end of the transcription factor combination sequence. The preparation method of the EMSA probe comprises the following steps of synthesizing a template strand and a primer which is labeled through biotin and modified through LNA, wherein the template strand contains the transcription factor combination sequence and the joint sequences located at the 5' end and the 3' end of the transcription factor combination sequence, each joint sequence is a palindromic sequence, and the nucleotide sequence of the primer is the same as the nucleotide sequence of each joint sequence; adopting the primer for conducting PCR amplification with the template strand as the template and synthesizing the EMSA probe . The EMSA probe can be used for conducting EMSA. According to the EMSA method, the probe thereof and the preparation method of the probe, the double-stranded probe is prepared from a single-strained probe through PCR, false negativeness due to incomplete renaturation is avoided, and developing errors caused by the single-stranded free probe are further overcome.

The invention provides a method for detecting a tumor biomarker by using a palindrome lock-type probe. A palindrome-sequence non-fluorescent mark lock-type RCA probe is designed. The probe is composedof a target identification part and a locus cutting part. The target identification part and target miRNA can completely complement each other and is located at a 3' end and a 5' end of the lock-typeprobe; a semi-identification locus of restriction enzyme Nt.AlWI is fused with a palindrome alkali segment. The probe is combined with nicking enzyme, an N-RCA concept is provided, a double-directionchain replacement reaction (D-SDA) is conducted for combination, and the probe is used for detecting the tumor marker miRNA. By means of the method, let-7a miRNA can be efficiently detected through amplification, and the probe is economical and practical and is expected to be applied to development of clinical diagnostic kits.

The invention provides a PCR primer and an application thereof in DNA fragment connection. The PCR primer comprises a palindrome sequence segment and any PCR primer sequence segment, the 3'end of thepalindrome sequence segment is connected with the 5'end of the any PCR primer sequence segment, the nucleotide of the 3' end of the palindrome sequence segment is U, and the any PCR primer sequence segment is complementarily paired with the 3'end of a pre-amplified DNA template. The primer provided by the embodiment of the invention can be used for constructing a sequencing library; in the construction process, the primer provided by the embodiment of the invention is used as a PCR amplification primer based on DNA, the two ends of an obtained amplification product DNA double strand have a palindrome sequence structure, and the 3'end of the palindrome sequence structure has nucleotide U, so that a foundation is laid for subsequent interconnection of different amplification product double strand DNAs.

The invention provides a system and method for synthesizing polynucleotides by solid phase assembly oligonucleotide precursors, in accordance with the method, a polynucleotide is partitioned into an ordered set of subunits, wherein each subunit is assembled in a single reaction from a subset of oligonucleotide precursors that uniquely anneal together to produce the subunit. The subunits are then assembled to form the desired polynucleotide. An important feature of the invention is the selection of subunits that are free of undesired sequence elements, such as palindromes, repetitive sequences, and the like, which would result in more than one subunit product alter ligating a pool of oligonucleotide precursors.

Belonging to the technical field of genetic engineering, the invention discloses a repetitive extragenic palindromic sequence for improving exogenous gene mRNA and application thereof. The repetitiveextragenic palindromic sequence and a spacer region sequence thereof are shown as SEQ ID NO, 1, the signal peptide can improve the stability of mRNA by changing the secondary structure, so that the extracellular enzyme activity of the target protein cyclodextrin glycosyltransferase can be improved by 14%, the recombinant escherichia coli modified by the signal peptide has strengthened extracellular protein production capacity, therefore the repetitive extragenic palindromic sequence can be used for escherichia coli to express exogenous protein.

The present invention discloses a method for modifying an amino acid attenuator, a class of amino acid attenuator mutants, engineered bacteria created on the basis of the amino acid attenuator mutants, and use of the engineered bacteria. The present invention protects a method for relieving the attenuation regulation of an amino acid operon gene, which is modification of the amino acid operon gene by: removing a gene coding for a leader peptide and an anterior reverse complementary palindromic sequence in the terminator stem-loop structure, and maintaining a posterior reverse complementary palindromic sequence in the terminator. The amino acid operon particularly can be histidine operon, tryptophan operon, phenylalanine operon, alanine operon, threonine operon and etc. The present invention can be used for the production of amino acids and derivatives thereof in fermentation by bacteria, providing a novel method for improving the production of amino acids in fermentation.

The invention provides a PCR primer and an application thereof in DNA fragment connection. The PCR primer comprises a palindrome sequence segment and any PCR primer sequence segment, the 3'end of thepalindrome sequence segment is connected with the 5'end of the any PCR primer sequence segment, the nucleotide of the 3' end of the palindrome sequence segment is U, and the any PCR primer sequence segment is complementarily paired with the 3'end of a pre-amplified DNA template. The primer provided by the embodiment of the invention can be used for constructing a sequencing library; in the construction process, the primer provided by the embodiment of the invention is used as a PCR amplification primer based on DNA, the two ends of an obtained amplification product DNA double strand have a palindrome sequence structure, and the 3'end of the palindrome sequence structure has nucleotide U, so that a foundation is laid for subsequent interconnection of different amplification product double strand DNAs.

The present invention provides a method for detecting tumor biomarkers using palindromic lock probes. A palindromic sequence non-fluorescence labeled lock RCA probe is designed. 3' end and 5' end of the formula probe; (2) Cutting site part: the half-recognition site of the restriction endonuclease Nt.AlWI, which is fused with a palindromic base fragment. It is combined with the nicking enzyme to propose the concept of N-RCA, and combined with the bidirectional strand displacement reaction (D-SDA), it is used for the detection of tumor marker miRNA. The invention can realize high-efficiency amplified detection of let-7a miRNA, is economical and is expected to be used in the development of clinical diagnostic kits.

Nucleic acid aptamers capable of binding to lymphocyte activation gene 3 (LAG-3) and uses thereof for modulating immune responses. Such aptamers may comprise a G-rich motif, for example, GX1GGGX2GGTX3A (SEQ ID No: 1), in which each of X1 and X2 are independently G, C, or absent, and X3 is T or C, or L- (G)n-L', in which n is an integer of 5-9 inclusive, and L and L'are nucleotide segments having complementary sequences. Also provided herein are multimeric nucleic acid aptamers containing a backbone moiety, which comprises a palindromic sequence.

The present invention provides a quality control method based on PacBio full-length transcriptome sequencing data, including steps: 1) using the IsoSeq analysis process to obtain high-quality and low-quality consistent full-length sequences from the original PacBio full-length transcriptome sequencing data; 2) Correct low-quality consensus full-length sequences based on Illumina sequencing data, and filter sequences that still fail to meet high-quality standards; 3) Merge high-quality and low-quality consistent full-length sequences that meet the conditions after correction, and filter according to the following criteria : Remove overly long sequences generated by sequence chimerism; remove consensus full-length sequences that have palindromic sequences in their own alignment results; remove sequences that can be aligned to multiple positions by other consistent full-length sequences. The chimeric sequences that may exist in the consensus full-length sequence are filtered through multiple criteria, reducing the proportion of false positive results in the final transcriptome and improving the accuracy of subsequent transcriptome-related analysis results.

The invention provides a nanoparticle based on cisplatin and DNA coordination self-assembly, and a preparation method and application thereof. The preparation method comprises the steps: dissolving cisplatin with normal saline under a high-temperature condition to prepare a cisplatin solution; then mixing DNA palindromic sequences with the cisplatin solution, and conducting a constant-temperaturereaction in a PCR instrument; and after annealing, supplementing an equal amount of DNA palindromic sequences, and continuously carrying out the constant-temperature reaction in the PCR instrument, soas to obtain nanoparticles with uniform shape and size. The DNA palindromic sequence is non-toxic and has good biocompatibility, self-crosslinking growth can occur through sequence design, and a hydrogel layer can be formed on the shell of the nanoparticle to improve the stability of the nanoparticle; meanwhile, cisplatin can be coated at the core of the nanoparticle by the DNA palindromic sequences to finally form the nanoparticle which is good in water solubility and relatively stable; the nanoparticle is simple in production process, environment-friendly in production process and relatively high in drug loading capacity, and has a wide application prospect in the field of drug delivery.