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A palindromic sequence is a nucleic acid sequence in a double-stranded DNA or RNA molecule wherein reading in a certain direction (e.g. 5' to 3') on one strand matches the sequence reading in the same direction (e.g. 5' to 3') on the complementary strand. This definition of palindrome thus depends on complementary strands being palindromic of each other.
Method for constructing nucleic acid library and use thereof
PCT designated stageWO2026050948A1Microbiological testing/measurementLibrary creationOrganic chemistryPalindromic sequence
Provided is a method for constructing a nucleic acid library. The method comprises: (a) providing a nucleic acid molecule comprising a self-amplifying adapter, the self-amplifying adapter having a palindromic sequence; (b) performing self-amplification on the nucleic acid molecule to obtain the nucleic acid library, wherein the self-amplification is realized by means of one or more cycles of a heating treatment and a cooling treatment, the palindromic sequence of the self-amplifying adapter is adapted for forming a hairpin structure, and the hairpin structure is adapted for serving as a primer for self-amplification.
Owner:SHENZHEN HUADA GENE INST