45 results about "Palindromic sequence" patented technology

A primer for the amplification of a DNA template comprising a protelomerase target sequence, particularly for production of closed linear DNA, which primer is capable of specifically binding to a palindromic sequence within a protelomerase target sequence and priming amplification in both directions.

The invention provides a system and method for synthesizing polynucleotides by solid phase assembly oligonucleotide precursors, in accordance with the method, a polynucleotide is partitioned into an ordered set of subunits, wherein each subunit is assembled in a single reaction from a subset of oligonucleotide precursors that uniquely anneal together to produce the subunit. The subunits are then assembled to form the desired polynucleotide. An important feature of the invention is the selection of subunits that are free of undesired sequence elements, such as palindromes, repetitive sequences, and the like, which would result in more than one subunit product alter ligating a pool of oligonucleotide precursors.

Belonging to the technical field of genetic engineering, the invention discloses a repetitive extragenic palindromic sequence for improving exogenous gene mRNA and application thereof. The repetitiveextragenic palindromic sequence and a spacer region sequence thereof are shown as SEQ ID NO, 1, the signal peptide can improve the stability of mRNA by changing the secondary structure, so that the extracellular enzyme activity of the target protein cyclodextrin glycosyltransferase can be improved by 14%, the recombinant escherichia coli modified by the signal peptide has strengthened extracellular protein production capacity, therefore the repetitive extragenic palindromic sequence can be used for escherichia coli to express exogenous protein.

The present invention discloses a method for modifying an amino acid attenuator, a class of amino acid attenuator mutants, engineered bacteria created on the basis of the amino acid attenuator mutants, and use of the engineered bacteria. The present invention protects a method for relieving the attenuation regulation of an amino acid operon gene, which is modification of the amino acid operon gene by: removing a gene coding for a leader peptide and an anterior reverse complementary palindromic sequence in the terminator stem-loop structure, and maintaining a posterior reverse complementary palindromic sequence in the terminator. The amino acid operon particularly can be histidine operon, tryptophan operon, phenylalanine operon, alanine operon, threonine operon and etc. The present invention can be used for the production of amino acids and derivatives thereof in fermentation by bacteria, providing a novel method for improving the production of amino acids in fermentation.

The present invention provides a method for detecting tumor biomarkers using palindromic lock probes. A palindromic sequence non-fluorescence labeled lock RCA probe is designed. 3' end and 5' end of the formula probe; (2) Cutting site part: the half-recognition site of the restriction endonuclease Nt.AlWI, which is fused with a palindromic base fragment. It is combined with the nicking enzyme to propose the concept of N-RCA, and combined with the bidirectional strand displacement reaction (D-SDA), it is used for the detection of tumor marker miRNA. The invention can realize high-efficiency amplified detection of let-7a miRNA, is economical and is expected to be used in the development of clinical diagnostic kits.

Nucleic acid aptamers capable of binding to lymphocyte activation gene 3 (LAG-3) and uses thereof for modulating immune responses. Such aptamers may comprise a G-rich motif, for example, GX1GGGX2GGTX3A (SEQ ID No: 1), in which each of X1 and X2 are independently G, C, or absent, and X3 is T or C, or L- (G)n-L', in which n is an integer of 5-9 inclusive, and L and L'are nucleotide segments having complementary sequences. Also provided herein are multimeric nucleic acid aptamers containing a backbone moiety, which comprises a palindromic sequence.

The present invention provides a quality control method based on PacBio full-length transcriptome sequencing data, including steps: 1) using the IsoSeq analysis process to obtain high-quality and low-quality consistent full-length sequences from the original PacBio full-length transcriptome sequencing data; 2) Correct low-quality consensus full-length sequences based on Illumina sequencing data, and filter sequences that still fail to meet high-quality standards; 3) Merge high-quality and low-quality consistent full-length sequences that meet the conditions after correction, and filter according to the following criteria : Remove overly long sequences generated by sequence chimerism; remove consensus full-length sequences that have palindromic sequences in their own alignment results; remove sequences that can be aligned to multiple positions by other consistent full-length sequences. The chimeric sequences that may exist in the consensus full-length sequence are filtered through multiple criteria, reducing the proportion of false positive results in the final transcriptome and improving the accuracy of subsequent transcriptome-related analysis results.