A method for detecting tumor biomarkers using palindromic lock probes

A technology of biomarkers and padlock probes, which is applied in the field of detection of tumor biomarkers using palindromic padlock probes, can solve the problems of high temperature control and insufficient sensitivity, and achieve high sensitivity and good specificity
CN108642137BInactive Publication Date: 2021-11-30FUZHOU UNIV
10 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
FUZHOU UNIV
Publication Date
2021-11-30
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention provides a method for detecting tumor biomarkers using palindromic lock probes. A palindromic sequence non-fluorescence labeled lock RCA probe is designed. 3' end and 5' end of the formula probe; (2) Cutting site part: the half-recognition site of the restriction endonuclease Nt.AlWI, which is fused with a palindromic base fragment. It is combined with the nicking enzyme to propose the concept of N-RCA, and combined with the bidirectional strand displacement reaction (D-SDA), it is used for the detection of tumor marker miRNA. The invention can realize high-efficiency amplified detection of let-7a miRNA, is economical and is expected to be used in the development of clinical diagnostic kits.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a method for detecting tumor biomarkers by using a palindromic lock probe. Background technique

[0002] Currently, many traditional amplification techniques, including blotting, real-time quantitative PCR, and microarrays, are widely used for miRNA detection. Although these methods have their advantages, at the same time, they have many limitations, such as insufficient sensitivity, strict temperature control, and high experimental expense. In order to overcome the above difficulties, we designed a palindromic sequence non-fluorescence-labeled locked RCA probe, combined with nicking cutting enzymes, proposed a new concept of N-RCA, and used it in conjunction with the double-way strand displacement reaction (D-SDA) for tumor Detection of marker miRNA. Contents of the invention

[0003] The purpose of the present invention is to provide a method for detecting tumor bio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More