46 results about "Palindrome" patented technology

The present invention belongs to the field of nucleic acid editing, and particularly the technical field of clustered regularly interspaced short palindromic repeats (CRISPRs). Specifically, provided in the present invention is a Cas mutant protein having an improved activity and an improved editing efficiency. Compared with a wild-type parental Cas protein, the Cas mutant protein of the present invention has significantly improved activity, and has a wide application prospect.

The invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of regularly clustered interval short palindromic repeat (CRISPR). Specifically, the invention provides a Cas12j mutant protein with improved editing activity and application thereof, and the Cas12j mutant protein has a wide application prospect.

The invention discloses a system for detecting viral nucleic acid on the basis of a CRISPR (clustered regularly spaced short palindromic repeat) technology and an FET (field effect transistor) chip and application of the system, and relates to a method for detecting the viral nucleic acid on the basis of clustered CRISPR (clustered regularly spaced short palindromic repeat) and related proteins (Cas) and a field effect transistor (FET). The CRISPR-FET (clustered regularly interspaced short palindromic repeats-field effect transistor) detection system comprises a non-amplified CRISPR system, a reporter RNA (ribonucleic acid) and gold nanoparticles (AuNPs) connection system, a separation system and a field effect transistor chip detection system. The CRISPR-FET detection method provided by the invention has high sensitivity and high specificity on virus nucleic acid detection, and the lower limit of detection reaches a single copy (100 copies / [mu] L).

The invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of regularly clustered interval short palindromic repeat (CRISPR). Specifically, the invention provides a Cas mutant protein with improved activity and improved editing efficiency, and compared with a wild parent Cas protein, the Cas mutant protein disclosed by the invention has the advantages that the activity is obviously improved, and the Cas mutant protein has a wide application prospect.

The invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of regularly clustered interval short palindromic repeat (CRISPR). Specifically, the invention provides the fusion protein, the fusion protein can be used for performing Prime Editing on target nucleic acid, and the fusion protein expands a guided editing system, so that the system can perform accurate editing while being compatible with a V-type CRISPR system, and has a wide application prospect.

The invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of regularly clustered interval short palindromic repeat (CRISPR). Specifically, the invention provides a Cas enzyme, and the Cas enzyme belongs to a class of Cas proteins and has a wide application prospect.

The invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of regularly clustered interval short palindromic repeat (CRISPR). Specifically, the invention provides adenosine deaminase which is fused with DNA binding protein, can be used for single base editing of target nucleic acid, and has a wide application prospect.

The invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of regularly clustered interval short palindromic repeat (CRISPR). Specifically, the invention provides a Cas mutant protein with improved activity and improved editing efficiency, and compared with a wild parent Cas protein, the Cas mutant protein disclosed by the invention has the advantages that the activity is obviously improved, and the Cas mutant protein has a wide application prospect.

A method for multiplex nucleic acid detection based on clustered regularly interspaced short palindromic repeat (CRISPR), a system, and a kit for detecting a target nucleic acid based on CRISPR are provided. The detection method includes: adding any one, any two, any three, or four from the group consisting of a first nucleic acid detection composition, a second nucleic acid detection composition, a third nucleic acid detection composition, and a fourth nucleic acid detection composition to a reaction system with a target nucleic acid to achieve the multiplex detection of the target nucleic acid.

The invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of regularly clustered interval short palindromic repeat (CRISPR). Specifically, the invention provides a Cas12j mutant protein with improved editing activity and application thereof, and the Cas12j mutant protein has a wide application prospect.

The invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of regularly clustered interval short palindromic repeat (CRISPR). Specifically, Cas9 or smaller Cas-SF01 (a Cas12i3 variant) is utilized to reasonably design and construct a compact mRNA-delivered epigenetic suppressor in an engineering manner, and an optimized mRNA structure, lipid nanoparticle (LNP) delivery, OFF-E-V2 mRNA optimized by single intravenous injection and selected guide RNA (gRNA) targeting mouse PCSK9 are combined, so that circulating PCSK9 protein is reduced by about 90%, corresponding LDL-C level is reduced by about 55%, and the PCSK9 protein is reduced by about 90%. The effect lasts for at least 180 days. Compared with a corresponding object based on Cas9, an editor based on Cas-SF01 shows higher specificity, and off-target methylation events are fewer. The optimized LNP formulations also exhibit good safety. These findings establish a powerful and versatile platform for promoting a transient delivery-based engineered mRNA editor to achieve precise and lasting epigenetic silencing in vivo therapy.

The present application relates to the field of nucleic acid editing, in particular to the technical field of clustered regularly interspaced short palindromic repeats (CRISPR). Specifically, provided are a Cas12j mutant protein having improved editing activity and the use thereof. The present application has wide prospects of application.

1. Name of the product in this design: Embossed shelf (palindrome). 2. Purpose of this design: For use in the production assembly of mobile shelving units. 3. The key design features of this product are its overall shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

Drug-inducible, tunable, and multiplexable Clustered Regularly Interspaced Short Palindromic Repeats from Prevotella and Francisella 1 (Cpf1)-based activators, and methods of use thereof.

The present invention belongs to the field of nucleic acid editing, in particular to the field of clustered regularly interspaced short palindromic repeats (CRISPR) technology. Specifically, the present invention provides a novel Cas enzyme, which has low homology with reported Cas enzymes, can exhibit nuclease activity inside and outside cells, and has broad application prospects.

The present disclosure relates to a method of constructing a synthetic enhancer comprising: identifying probable palindromic subsequences in a promoter of interest; selecting and extracting highly palindromic subsequences from amongst the probable palindromic subsequences; and concatenating some or all of the extracted highly palindromic subsequences to produce a synthetic enhancer. Identifying the probable palindromic subsequence includes defining a candidate subsequence in the promoter of interest; generating a complement or reverse complement of the candidate subsequence; comparing the candidate subsequence with its complement or reverse complement to identify the number of mismatches; and identifying the candidate subsequence as a probable palindromic subsequence if the number of mismatches is the same or lower than a mismatch threshold corresponding to the number of mismatches expected from comparable randomly generated sequences. The method can be applied to create a synthetic enhancer for any promoter of interest.

The invention discloses a data processing method and device, electronic equipment and a storage medium, and relates to the technical field of computers, character strings are divided in multiple dynamic division modes with different character lengths, palintext character strings in sub-character strings are found out, the division mode containing the most palintext character strings is used as a target division mode, and the target division mode is used for dividing the palintext character strings in the sub-character strings. According to the method, repeated contents in the data can be found to the greatest extent by maximizing the number of the palindromic character strings, so that repeated parts in the character strings can be accurately identified and removed, and the characteristics of the palindromic character strings are fully utilized to optimize a data storage structure. Therefore, the technical problem of high cost of data storage equipment caused by increase of the total data amount can be solved, and the technical effects of improving the storage utilization rate, reducing the number and capacity requirements of the storage equipment and further reducing the operation cost of enterprises are achieved.

Disclosed herein are Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) systems for use as a medicament, the CRISPR system comprising a guide RNA (gRNA) comprising the sequence set forth in any one of SEQ ID NO: 3-74, and a CRISPR-associated endonuclease, the gRNAs targeting the Neh4 and / or Neh5 domain of NRF2. Also disclosed herein are methods of using the aforementioned gRNAs, DNA sequences encoding such gRNAs, and vectors and pharmaceutical compositions comprising such gRNAs.

The invention discloses a palindromic mediated self-assembly DNA molecule as well as a preparation method and application thereof, and belongs to the technical field of biology. The palindromic mediated self-assembled DNA molecule is obtained by self-assembling an A1 chain, an L1 chain and a C1 chain; the nucleotide sequence of the chain A1 is as shown in SEQ ID NO. 1; the nucleotide sequence of the L1 chain is as shown in SEQ ID NO. 2; and the nucleotide sequence of the C1 chain is as shown in SEQ ID NO. 3. The palindromic mediated self-assembled DNA molecule A1-L1-C1 disclosed by the invention can be used for detecting miR-21, and has relatively high detection sensitivity and specificity. The A1-L1-C1 also has a drug loading function, and can target specific tumor cells and accurately deliver drugs into cancer cells. The invention provides a new technical approach for early diagnosis of tumors, detection of intracellular biomolecules and targeted drug delivery, and has a wide application prospect.

The invention provides an arrhythmia risk grading detection method based on a CNN-LSTM palindromic network structure, and the method comprises the following steps: 1, integrating data of a plurality of electrocardiogram public data sets, extracting complete rhythm segments, and forming a rhythm data set which comprises 16 rhythms; 2, dividing the 16 rhythms in the step 1 into three types of rhythm sections according to criticality, and setting the three types of rhythm sections to be normal, need to be focused and criticality-real-time response levels respectively; and step 3, intercepting specific lengths of the three types of rhythm sections, dividing a training set and a test set by adopting a ten-fold cross validation method, and verifying the detection precision. According to the detection method provided by the invention, multi-level and multi-scale more comprehensive spatial-temporal characteristics can be obtained, and a more accurate prediction result can be obtained.

Embodiments of this application disclose a slot allocation method and a related device, which are applied to a network carrying fixed services. The slot allocation method includes: determining a palindromic sequence based on a total quantity of available slots. A quantity of bases in the palindromic sequence is the same as the total quantity of available slots. That is, a length of the palindromic sequence is the same as the total quantity of slots, and a slot allocation result obtained based on the palindromic sequence corresponds to the total quantity of slots. Therefore, there is no need to introduce an idle service or a queuing mechanism. This reduces hardware overheads generated during slot allocation.

The present invention relates to an engineered Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) system comprising engineered dual guide nucleic acids (e.g., RNAs) capable of activating a CRISPR-Associated (Cas) nuclease, such as a type V-A Cas nuclease. Also provided are methods of targeting, editing, and / or modifying a nucleic acid using the engineered CRISPR system, and compositions and cells comprising the engineered CRISPR system.

Disclosed herein are Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) / CRISPR-associated (Cas) 9-based system related compositions and methods of using said CRISPR / Cas9-based system related compositions for altering gene expression and genome engineering. Also disclosed herein are compositions and methods of using said compositions for altering gene expression and genome engineering in muscle, such as skeletal muscle and cardiac muscle.

The present invention generally relates to libraries, compositions, methods, applications, kits and screens used in functional genomics that focus on gene function in a cell and that may use vector systems and other aspects related to Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas systems and components thereof. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system.

The present application belongs to the field of nucleic acid editing, in particular, the field of clustered regularly interspaced short palindromic repeats (CRISPR) technology. Specifically, the present application provides a Cas mutant protein with improved activity and increased editing efficiency. Compared with the wild-type parent Cas protein, the Cas mutant protein of the present application has significantly improved activity and has a wide application prospect.

The present invention belongs to the field of biotechnology and discloses a method and application of MMEJ targeted genome modification based on palindromic sequences within the target site. The present invention uses the CRISPR / Cas9 system to form a 6bp microhomology sequence after reverse complementation of the same target site, which can be used to mark endogenous modification of fish genes. Taking zebrafish as an example, the applicant has targeted genes specifically expressed in the zebrafish heart. myl7 Perform targeted genome modification to establish a transgenic strain of zebrafish heart-specific marker green fluorescent protein. MMEJ-targeted genome modification mediated by palindromic sequences within the target site is a precise and efficient repair method that achieves tissue-specific labeling without destroying the function of endogenous genes, and truly, accurately and sensitively reflects gene expression. myl7 The molecular expression level of myl7 The study of gene function.

The invention belongs to the technical field of biology, and relates to novel effect nuclease and application thereof, the novel effect nuclease related to regular clustered interval short palindromic repeat (CRISPR) is obtained according to a discovered and researched Cas12a effect nuclease sequence, design, screening and functional verification, and specifically, the invention provides a Cas protein which is Cas-kf63 or Cas-kf66, and the Cas-kf63 or Cas-kf66 is a Cas-kf63 gene or a Cas-kf66 gene or a Cas-kf66 gene or a Cas-kf63 gene or a Cas-kf66 gene or a Cas-kf66 gene. The amino acid sequence of the Cas-kf63 is as shown in SEQ ID No 1; the amino acid sequence of the Cas-kf66 is as shown in SEQ ID No4. The Cas protein provided by the invention shows the activity of nuclease through experiments, and has a wide application prospect.

The present application belongs to the field of nucleic acid editing, in particular, the field of clustered regularly interspaced short palindromic repeats (CRISPR) technology. Specifically, the present application provides a Cas mutant protein with improved activity and increased editing efficiency. Compared with the wild-type parent Cas protein, the Cas mutant protein of the present application has significantly improved activity and has a wide application prospect.

The invention belongs to the field of nucleic acid editing, and particularly relates to the technical field of regularly clustered interval short palindromic repeat (CRISPR). Specifically, the invention provides adenosine deaminase which is fused with DNA binding protein, can be used for single base editing of target nucleic acid, and has a wide application prospect.