36 results about "Palindrome" patented technology

Disclosed herein are Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) / CRISPR-associated (Cas) 9-based system related compositions and methods of using said CRISPR / Cas9-based system related compositions for altering gene expression and genome engineering. Also disclosed herein are compositions and methods of using said compositions for altering gene expression and genome engineering in muscle, such as skeletal muscle and cardiac muscle.

A novel immunostimulatory oligonucleotide by which an IFN-inducing activity is enhanced and an inflammatory cytokine-inducing activity is reduced, and a pharmaceutical containing the same, and an application thereof are provided. That is, the present invention provides the immunostimulatory oligonucleotide composed of a base sequence represented by a formula: 5′-(G)MPXCGYQ(G)N-3′ (SEQ ID NO: 118) (X and Y are mutually independent and represent an arbitrary sequence which has a length of 0 to 10 nucleotides and does not contain 4 or more consecutive G residues, and a length of X+Y is 6 to 20 nucleotides; XCGY contains a palindrome sequence having a length of at least 8 nucleotides and has a length of 8 to 22 nucleotides; P and Q are mutually independent and represent one nucleotide other than G; M represents an integer of 6 to 10 and N represents an integer of 0 to 3) wherein a full length thereof is 16 to 37 nucleotides (except for an oligonucleotide composed of a base sequence represented by SEQ ID NO:5), the pharmaceutical application thereof.

The invention relates to a fluorescent probe for fluorescent quantitative PCR reactions. The fluorescent probe is characterized in that the probe comprises an oligonucleotide sequence, the 5' terminus is a specific oligonucleotide sequence with an amplified target sequence and modifies the fluorescence groups; the 3' terminus is a nucleotide sequence, forms a reversely-complimentary palindrome structure with the 5' terminus, and modifies the fluorescence quenching groups. Under certain conditions, the probe can form an intramolecular secondary composition, and the fluorescent groups get close to the quenching groups so as to quench the fluorescence signals released by the fluorescence groups. Compared to the common fluorescent probe, the provided fluorescent probe has the advantages of high specificity, high sensitivity, and low fluorescent quenching efficiency, thus the signal to noise ratio (SNR) of the reactions is higher, and the provided fluorescent probe can be applied to realtime fluorescence quantitative PCR detection reactions and all end point fluorescence detection reactions.

The invention provides a method for detecting a tumor biomarker by using a palindrome lock-type probe. A palindrome-sequence non-fluorescent mark lock-type RCA probe is designed. The probe is composedof a target identification part and a locus cutting part. The target identification part and target miRNA can completely complement each other and is located at a 3' end and a 5' end of the lock-typeprobe; a semi-identification locus of restriction enzyme Nt.AlWI is fused with a palindrome alkali segment. The probe is combined with nicking enzyme, an N-RCA concept is provided, a double-directionchain replacement reaction (D-SDA) is conducted for combination, and the probe is used for detecting the tumor marker miRNA. By means of the method, let-7a miRNA can be efficiently detected through amplification, and the probe is economical and practical and is expected to be applied to development of clinical diagnostic kits.

The invention relates to a Leon-RC compression method for genome sequencing data. The method mainly aims to improve the steps of constructing an anchor point dictionary by an LEON algorithm. The method includes the following steps that: (1) a short-read is divided into a plurality of Kmers; (2) one Kmer is selected, the Kmer values of the direct repetition, mirror repetition, inversion repetition,and complementary palindrome of the Kmer are calculated, the four values are compared with one another, so that a minimum Kmer value can be obtained; (3) the minimum Kmer value is inputted into a Bloom filter for matching search, a Solid Kmer is stored in the Bloom filter, and whether the minimum Kmer value exists in the Solid Kmer is judged; if the minimum Kmer value exists in the Solid Kmer, the minimum Kmer value is added to the anchor point dictionary, and search is ended; if the minimum Kmer value does not exist in the Solid Kmer, the next Kmer is obtained, and the step (2) and step (3)are repeated; (4) if the minimum Kmer values of all the Kmer do not exist in the Solid Kmer, it is indicated that no anchor point exists in the short-read; and (5) the anchor point dictionary is constructed through the step (1) to (4).

The invention provides a PCR primer and an application thereof in DNA fragment connection. The PCR primer comprises a palindrome sequence segment and any PCR primer sequence segment, the 3'end of thepalindrome sequence segment is connected with the 5'end of the any PCR primer sequence segment, the nucleotide of the 3' end of the palindrome sequence segment is U, and the any PCR primer sequence segment is complementarily paired with the 3'end of a pre-amplified DNA template. The primer provided by the embodiment of the invention can be used for constructing a sequencing library; in the construction process, the primer provided by the embodiment of the invention is used as a PCR amplification primer based on DNA, the two ends of an obtained amplification product DNA double strand have a palindrome sequence structure, and the 3'end of the palindrome sequence structure has nucleotide U, so that a foundation is laid for subsequent interconnection of different amplification product double strand DNAs.

The invention provides a system and method for synthesizing polynucleotides by solid phase assembly oligonucleotide precursors, in accordance with the method, a polynucleotide is partitioned into an ordered set of subunits, wherein each subunit is assembled in a single reaction from a subset of oligonucleotide precursors that uniquely anneal together to produce the subunit. The subunits are then assembled to form the desired polynucleotide. An important feature of the invention is the selection of subunits that are free of undesired sequence elements, such as palindromes, repetitive sequences, and the like, which would result in more than one subunit product alter ligating a pool of oligonucleotide precursors.

Belonging to the technical field of genetic engineering, the invention discloses a repetitive extragenic palindromic sequence for improving exogenous gene mRNA and application thereof. The repetitiveextragenic palindromic sequence and a spacer region sequence thereof are shown as SEQ ID NO, 1, the signal peptide can improve the stability of mRNA by changing the secondary structure, so that the extracellular enzyme activity of the target protein cyclodextrin glycosyltransferase can be improved by 14%, the recombinant escherichia coli modified by the signal peptide has strengthened extracellular protein production capacity, therefore the repetitive extragenic palindromic sequence can be used for escherichia coli to express exogenous protein.

The invention provides a PCR primer and an application thereof in DNA fragment connection. The PCR primer comprises a palindrome sequence segment and any PCR primer sequence segment, the 3'end of thepalindrome sequence segment is connected with the 5'end of the any PCR primer sequence segment, the nucleotide of the 3' end of the palindrome sequence segment is U, and the any PCR primer sequence segment is complementarily paired with the 3'end of a pre-amplified DNA template. The primer provided by the embodiment of the invention can be used for constructing a sequencing library; in the construction process, the primer provided by the embodiment of the invention is used as a PCR amplification primer based on DNA, the two ends of an obtained amplification product DNA double strand have a palindrome sequence structure, and the 3'end of the palindrome sequence structure has nucleotide U, so that a foundation is laid for subsequent interconnection of different amplification product double strand DNAs.

The invention relates to a microfluidic-technology-based CTC protein typing kit. The kit comprises: (1) capture antibody compounds aiming at different CTCs marker protein, each capture antibody compound being made from a capture antibody in specific binding to corresponding marker protein and an aptamer, wherein the capture antibody and aptamer are connected through a cuttable material which is deoxyribonucleotide double strands having a palindrome structure and two or more than two restriction enzyme sites; and (2) a microfluidic chip, the surface of which is coupled to a cell capture assembly. The cell capture assembly made from a microcolumn is fixed at the surface of the microfluidic chip, and a specific conjugate capable of bonding to the aptamer modifies one end of the microcolumn. Through the microfluidic technology, efficient and fast automatic detection and typing of CTCs is achieved, the kit is simple to operate, false-negative and false-positive results due to man-made operational factors are reduced, and the sensitivity and accuracy of detection are improved.

The present invention provides a method for detecting tumor biomarkers using palindromic lock probes. A palindromic sequence non-fluorescence labeled lock RCA probe is designed. 3' end and 5' end of the formula probe; (2) Cutting site part: the half-recognition site of the restriction endonuclease Nt.AlWI, which is fused with a palindromic base fragment. It is combined with the nicking enzyme to propose the concept of N-RCA, and combined with the bidirectional strand displacement reaction (D-SDA), it is used for the detection of tumor marker miRNA. The invention can realize high-efficiency amplified detection of let-7a miRNA, is economical and is expected to be used in the development of clinical diagnostic kits.

Methods for gene editing and predicting non-random editing events are described. The methods use Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems to characterize and manipulate DNA repair outcomes at Cas-initiated double-strand breaks (DSBs) to anticipate functional outcomes.

The invention discloses a pseudo attP site in the swine genome and applications thereof, belonging to the technical field of biotechnology and biomedical engineering. According to the invention, a pseudo attP site is isolated from the swine genome, and the pseudo attP site has a sequence similarity of 40% with the wild type attP site and has the characteristic of palindromic sequence. The pseudo attP site can be recognized by Streptomycete bacteriophage phi C 31 intergrase, and thereby exogenous genes having attP site can be mediated to integrate in the site in relatively high efficiency. According to protein content tests, the expression level of EGFP gene maintained by the pseudo attP site is 50 times that by the genome without exogenous genes. The invention provides a new technical scheme for targeted integration and high efficiency expression for swine transgene, and a new strategy for the research of swine functional genome, so the invention has important science value and broad application prospect.

The invention relates to a Leon-RC compression method for genome sequencing data, which mainly improves the steps of constructing an anchor dictionary by the LEON algorithm, including the following steps: (1) dividing short reads into multiple Kmers; (2) selecting one Kmer, calculate the Kmer value of its direct repetition, mirror repetition, inverted repetition, and complementary palindrome, compare these four values, and obtain the smallest Kmer value; (3) Put the smallest Kmer value into the Bloom filter for matching Search, Solid Kmer is stored in the Bloom filter, and judge whether there is a minimum Kmer value in Solid Kmer; if it exists, add the minimum Kmer value to the anchor dictionary and end the search; if not, obtain the next One Kmer, repeat steps (2), (3); (4) If the minimum Kmer value of all Kmers does not exist in Solid Kmer, it means that there is no anchor point for this short read; (5) Pass step (1) ~(4) Construct the anchor dictionary.

The invention discloses an EMSA method, a probe thereof and a preparation method of the probe. The EMSA probe comprises a first strip chain, and the first strip chain comprises a transcription factor combination sequence and joint sequences located at the 5' end and the 3' end of the transcription factor combination sequence. The preparation method of the EMSA probe comprises the following steps of synthesizing a template strand and a primer which is labeled through biotin and modified through LNA, wherein the template strand contains the transcription factor combination sequence and the joint sequences located at the 5' end and the 3' end of the transcription factor combination sequence, each joint sequence is a palindromic sequence, and the nucleotide sequence of the primer is the same as the nucleotide sequence of each joint sequence; adopting the primer for conducting PCR amplification with the template strand as the template and synthesizing the EMSA probe . The EMSA probe can be used for conducting EMSA. According to the EMSA method, the probe thereof and the preparation method of the probe, the double-stranded probe is prepared from a single-strained probe through PCR, false negativeness due to incomplete renaturation is avoided, and developing errors caused by the single-stranded free probe are further overcome.

Disclosed herein are clustered regularly interspaced short palindromic repeat (CRISPR) / CRISPR-associated (Cas)9-based system-related compositions and use of said CRISPR / Cas9-based system-related compositions for altering gene expression and genome engineering The method of transformation. Also disclosed herein are compositions and methods for altering gene expression and genome engineering in muscles, such as skeletal and cardiac muscle, using the compositions.