Palindrome molecular beacon-based biosensor for detecting ATP and fabrication method and application of biosensor

A biosensor and molecular beacon technology, applied in the field of biosensors based on palindromic molecular beacons to detect ATP, can solve the problems of high cost, long detection cycle, low specificity and sensitivity, and achieve low detection limit and fast detection speed , cheap effect
CN110609020AActive Publication Date: 2019-12-24UNIV OF JINAN
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
UNIV OF JINAN
Publication Date
2019-12-24

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Abstract

The invention relates to the technical field of biosensors, in particular to a palindrome molecular beacon-based biosensor for detecting adenosine triphosphate (ATP). The palindrome molecular beacon-based biosensor comprises a palindrome molecular beacon MB, an ATP adaptor (split adaptor probes AP1 and AP2), target object ATP, Bst DNA polymeras, UDG, endonuclease IV and a buffer liquid. Accordingto specificity identification of an aptamer and the target object, fragment tail sequences of the two split adaptors get close to each other, a hairpin structure of the palindrome molecular beacon MBis opened to generate fluorescence, at the moment, a palindrome sequence locked in advance is released, intermolecular hybridization is performed, so that the spontaneous polymerization, repair, internal cut and circulation process is triggered, the amplification of a fluorescent signal is achieved, and an aptamer biosensor is built. The sensor reaction only needs a step, thus, the biosensor has the advantages of rapid detection speed, low cost, low detection limit, high specificity and the like and is simple and convenient to operate.
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Description

technical field

[0001] The invention relates to the technical field of biosensors, in particular to a biosensor for detecting ATP based on a palindromic molecular beacon, and also relates to a preparation method and application thereof. Background technique

[0002] Adenosine triphosphate (ATP), present in all organisms from microorganisms to higher animals and plants, is the main energy source of cells and plays an important role in intracellular metabolic, synthetic and biochemical processes. The content of ATP in the body is not high, and the molar concentration in cells is usually 1-10 mM. Dead cells will not be able to detect ATP, and the ATP metabolism of cancer cells is particularly active. Usually, abnormal ATP concentration is closely related to many diseases, such as hypoglycemia, cardiovascular disease, Parkinson's, tissue hypoxia, etc. Therefore, the rapid and accurate determination of ATP is of great significance for the study of the physiological activity and ...

Claims

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