Method for preparing high molecular fluorescent microballoons

Abstract

Disclosed is a method for preparing fluorescent polymer beads, belonging to methods for preparing nano-material and micron material and aiming at providing a preparation method which requires no strict conditions and is easy and convenient to operate, and the raw materials are safe, easy to get, low in cost and high in reproducibility and efficiency. The method includes the following steps:(1)preparing stabilizing agent solution; (2) preparing monomer solution; (3)blending for reaction; (4)cleaning repeatedly for 3-5times; the deposit finally got is fluorescent beads. The invention is capable of preparing a plurality of fluorescent beads with even granularity distribution through dispersion-polymerization in one step; requirements to fluorescent dye are low, and the fluorescent dye is low in cost and is of no need to change the intrinsical structure of the fluorescein, thus avoiding changing the fluorescence and solving the problem that the fluorescent dye is easy to lose in direct adsorption method. The monodisperse particles of the fluorescent polymer beads prepared through the method are 1-5 microns and the fluorescent polymer beads are of high reproducibility and high efficiency and of no special compatibility to biological tissue cells.

Description

technical field [0001] The invention belongs to a method for preparing nano and micro materials, in particular to a method for preparing polymer fluorescent microspheres by a dispersion polymerization method. Background technique [0002] Fluorescent microspheres refer to solid particles with a diameter ranging from nanometer to micrometer, loaded with fluorescent substances, and can be stimulated by external energy to emit fluorescence. The shape can be any shape, and the typical shape is spherical. The carriers of fluorescent microspheres are mostly organic or inorganic polymer materials, which have relatively stable morphological structure and luminescent behavior, and are less affected by external conditions such as solvents, heat, electricity, and magnetism than pure fluorescent compounds. As a new type of carrier material, it has been widely used in the field of biomedicine, such as directional delivery of drugs in biochemistry and biomedicine, medical latex reagents, ...

AI Technical Summary

Problems solved by technology

However, the fluorescent microspheres prepared by the above two methods have stable fluorescence content and low toxicity, but these two methods have specific requirements for the monomer, which makes the price of the reagent very high. In addition, most of the fluoresceins contain benzene rings and hetero The rigid planar structure of the conjugated double bond of the ring makes it difficult for the chemical reaction to proceed. In addition, the chemical reaction changes the structure of the fluorescent substance, thereby changing the properties of the fluorescent substance, such as red shift

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