Mitochondrion tRNA (transfer ribonucleic acid)<Thr> 15909A>G mutation detection method and kit thereof

Abstract

The invention discloses a mitochondrion tRNA (transfer ribonucleic acid)<Thr> 15909A>G mutation detection method and a kit for detecting mitochondrion tRNA<Thr> 15909A>G mutation. The method includes DNA (deoxyribonucleic acid) extraction, specific primer design, specific band PCR (polymerase chain reaction) amplification, specific restriction endonuclease HpyCH4V enzyme digestion, agarose gel electrophoresis identification and the like. After being subjected to specific PCR amplification, a DNA sample positioned at a tRNA<Thr> 15909A>G mutation position possibly comprises a TGCA palindrome structure which can be specifically recognized by specific restriction endonuclease HpyCH4V, then two small fragments are obtained by rapid enzyme digestion, and two bands are obtained after agarose gel electrophoresis. The mitochondrion tRNA<Thr> 15909A>G mutation detection method and the kit have the advantages that requirements on required detection instruments and required detection reagent cost are low, and according to the required method, simplicity and stability in operation, easiness in rapid mastering, accuracy, good specificity and the like are achieved; the method and the kit are beneficial to establishment of perfect screening technology for tRNA<Thr> mutation and can be widely popularized to clinical detection.

Description

technical field [0001] The invention belongs to the field of mitochondrial tRNA mutation detection, in particular to a mitochondrial tRNA Thr 15909A>G mutation detection method, kit and application. Background technique [0002] Cardiovascular disease is the leading cause of death in the world. According to the statistics of the World Health Organization, about 17.3 million people died of cardiovascular disease in 2008. By 2030, almost 23.3 million people will die of cardiovascular disease. Hypertension is number one risk factor for cardiovascular disease. High blood pressure affects more than one-third of adults worldwide, or about 1 billion people, and causes more than 9 million deaths worldwide each year, half of whom die from heart disease and stroke. High blood pressure can also cause kidney failure, blindness, Broken blood vessels and brain damage. Hypertension can be divided into primary hypertension and secondary hypertension according to the cause of the disea...

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Problems solved by technology

[0006] To solve the above problems, the present invention provides a mitochondrial tRNA Thr 15909A>G mutation method, mitochondrial tRNA by enzymatic digestion Thr 15909A>G mutation detection overcomes the shortcomings of existing methods such as time-consuming, difficult operation, and high cost, and provides mitochondrial tRNA Thr 15909A>G mutation detection kit, and the above method or the above kit in the detection and mitochondrial tRNA Thr Application of 15909A>G Mutation in Detection of Mutation

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