pcr primers and their application in ligation of dna fragments

A technology of primer sequences and products, applied in DNA/RNA fragments, DNA preparation, recombinant DNA technology, etc., can solve the problems of short sequencing sequences, short sequence splicing cannot provide long transcripts, loss of alternative splicing, etc.
CN111748611BActive Publication Date: 2021-02-19BGI TECH SOLUTIONS
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Patents(China)
Current Assignee / Owner
BGI TECH SOLUTIONS
Publication Date
2021-02-19

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Abstract

The invention provides a PCR primer and an application thereof in DNA fragment connection. The PCR primer comprises a palindrome sequence segment and any PCR primer sequence segment, the 3'end of thepalindrome sequence segment is connected with the 5'end of the any PCR primer sequence segment, the nucleotide of the 3' end of the palindrome sequence segment is U, and the any PCR primer sequence segment is complementarily paired with the 3'end of a pre-amplified DNA template. The primer provided by the embodiment of the invention can be used for constructing a sequencing library; in the construction process, the primer provided by the embodiment of the invention is used as a PCR amplification primer based on DNA, the two ends of an obtained amplification product DNA double strand have a palindrome sequence structure, and the 3'end of the palindrome sequence structure has nucleotide U, so that a foundation is laid for subsequent interconnection of different amplification product double strand DNAs.
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Description

technical field

[0001] The present invention relates to the field of biotechnology. Specifically, the present invention relates to PCR primers and their application in DNA fragment ligation. More specifically, the present invention relates to PCR primers, PCR amplification methods, methods for constructing sequencing libraries, and full-length transcriptomes. sequencing method. Background technique

[0002] Genome and transcriptome sequencing is fundamental work in the life sciences. Since the vast majority of non-model organisms lack genomic data, full-length transcriptome sequencing has become particularly important. Full-length transcripts can greatly promote basic and applied research on gene function, gene expression regulation, and evolutionary relationships in these species. Currently, the vast majority of transcriptome data are obtained based on second-generation high-throughput sequencing technologies. However, the sequencing sequence of the second-generation sequ...

Claims

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