9 results about "Mutation gene" patented technology

The application belongs to the field of plant genetic engineering, and particularly relates to application of a rice leaf albino gene OsLCD2 in regulating rice leaf color traits, and further provides a low-temperature sensitive rice leaf albino gene oslcd2. The OsLCD2 gene and the oslcd2 gene provided by the application have application prospects in hybrid F1 generation hybrid purity identification and sterile line self-cross propagation seed purity identification in rice breeding, and are of great significance in improving seed purity and reducing hybrid rice production risks.

This invention discloses a prognostic and therapeutic suitability assessment model for hepatocellular carcinoma (HCC) based on mRNA vaccine antigens, and its construction method. First, the differences in gene expression between normal tissues and HCC tumors are analyzed to understand the mutations and genomic structural changes in HCC patients. Then, genes related to the level of antigen-presenting cell infiltration, as well as genes significantly related to overall survival and disease-free survival, are further selected from anomalously expressed and mutated genes to obtain candidate mRNA vaccine neoantigen targets. Based on the expression levels of these targets, patients are immunophenotyped to assess the patient population suitable for mRNA vaccines. Simultaneously, the relationship between target expression levels and patient prognosis is quantified to predict the probability of HCC patients achieving 3-year and 5-year overall survival. This invention can objectively and accurately assess treatment resistance and tumor immune status in HCC, improving the predictive accuracy of HCC treatment prognosis.

The invention discloses a high-sensitivity tumor mutation detection method based on a synergistic primer co-F. The high-sensitivity tumor mutation detection method is mainly based on a synergistic amplification reaction (Co-PCR) of the synergistic primer co-F. Wherein the synergistic primer co-F is formed by connecting a mutation probe modified by azide and a short primer modified by dibenzocyclooctyne (DBCO) through a copper-free click chemical reaction, and comprises two core modules, namely the mutation probe and the short primer. Wherein the mutation probe module is responsible for high-specificity recognition and anchoring of a mutation gene sequence, and the short primer module with a relatively low melting temperature (Tm) value is accurately positioned and hybridized to a position near a target area of a mutation target under the guidance of a correct hybridization mutation target of the mutation probe module, so that amplification is started; meanwhile, the design utilizes the characteristic of low melting temperature of the short primer, so that the short primer cannot independently initiate reaction when the mutation probe module is not combined with the mutation template, thereby inhibiting the non-specific amplification of the wild type template, and finally achieving high-sensitivity and high-selectivity amplification of the mutation DNA.

The application relates to the technical field of genetic engineering, and discloses application of genes NAT2 and NAT3 in improving heat resistance of rice. The genomic DNA sequence of the gene NAT2 is shown as SEQ ID NO. 1, and the genomic DNA sequence of the gene NAT3 is shown as SEQ ID NO. 2. The survival rate of the obtained gene editing plants at the seedling stage and the seed setting rate of the plants at the reproductive stage are improved under high-temperature stress conditions by gene editing mutation of the genes NAT2 and NAT3, and the yield of the rice under extreme high-temperature conditions is protected.

This invention relates to the field of agricultural technology, specifically to a mutant Arabidopsis protein and gene that promotes chloroplast development, and their applications. The mutant Arabidopsis protein is the AtMurE protein in wild-type Arabidopsis Sig6 factor-deficient mutants, with mutations occurring at positions 89, 97, 106, 125, 126, 130, or 140. The mutant protein or gene provided by this invention, when introduced into wild-type Arabidopsis Sig6 factor-deficient mutants, can restore chlorophyll content and resolve the cotyledon yellowing problem in wild-type Arabidopsis Sig6 factor-deficient mutants.

The application discloses a gene panel for detecting retinal pigment degeneration and application thereof, the gene panel comprising PRPF31, TFPT, NDUFA3, OSCAR, USH2A, EYS, RPGR, RHO, RP1, ABCA4, RDH12, CRB1, CNGA1, SNRNP200, CERKL, PDE6B, PROM1, CEP290, RP2, CYP4V2, RPE65, PRPF6 and CNGB1. The gene panel comprises PRPF31 and its upstream and downstream genes, and covers high-frequency mutation genes in an RP population. Through high-density probe design on target regions of the genes, not only the detection cost is reduced, but also precise diagnosis of clinical typing of RP and comprehensive genetic evaluation of PRPF31-RP patients can be realized.

The application belongs to the technical field of molecular biology and crop breeding, and particularly relates to a STS marker completely linked with wheat heading stage and plant height, a primer, a distinguishing method and application thereof in breeding. The STS marker completely linked with wheat heading stage and plant height is G base insertion at the 68416769 base of wheat 7D chromosome. The application further provides a primer capable of amplifying the STS marker, and the primer comprises sequences as shown in SEQ ID NO:1 and SEQ ID NO:2 respectively. The STS marker is a functional molecular marker of wheat heading stage and plant height mutation gene, and can be used for molecular marker assisted selection and screening of wheat breeding materials. The site identified by the functional STS marker in the wild type and mutant can be used for improving the heading stage and plant height of wheat through backcross selection.

The invention discloses a probe set for evaluating whole-body tumor load of small cell lung cancer and application of the probe set, the probe set comprises a plurality of oligonucleotide probes for specifically capturing mutation regions of SCLC related genes in a targeted mode, and the genes are selected from TP53, RB1, CREBBP, EGFR, PTEN, PIK3CA, LRP1B and NOTCH1; and the probe set is capable of covering at least 94.9% of at least one mutation existing in an SCLC patient sample. The invention relates to the technical field of biotechnology and molecular diagnosis, and has the beneficial effects that based on a clinical queue, the probe group incorporates high-frequency mutant genes of SCLC, can be superposed with personalized probes for use, and is used for ctDNA detection of SCLC patients and evaluation of whole-body tumor load. The SCLC cancer species specific probe group provided by the invention plays a role in monitoring tumor evolution and new mutation, can overcome the space-time heterogeneity of tumors to a certain extent, and also can improve the capture efficiency at the same time.

The invention discloses a KASP primer pair for identifying wheat ear types as well as an identification method and application of the KASP primer pair. The KASP primer pair comprises an upstream primer and a downstream primer, wherein the nucleotide sequences of the upstream primer 1 and the upstream primer 2 are respectively as shown in SEQ ID No. 1 and SEQ ID No. 2; and the nucleotide sequence of the downstream primer is as shown in SEQ ID No.3. The KASP marker is a wheat ear type mutant gene functional molecular marker, and the SNP site corresponding to the KASP molecular marker is that G located at the 57941628th basic group of a wheat 1D chromosome is mutated into A. The technical scheme provided by the invention can be used for molecular marker-assisted selection and screening of wheat ear type new materials. The KASP primer pair provided by the invention can be used for simply, conveniently and quickly detecting and identifying the ear type of wheat at high throughput, and the ear type improvement of wheat can be accelerated through backcross selection.