The invention belongs to the technical field of livestock immunology, and concretely relates to a pig tissue chromatin immunoprecipitation processing method. The method is named as ChIP for short. The method specially aims at pig tissue for optimization design, employs a tissue lysate and a Dounce grinder for combination, and develops the method, the processes and the flows of pig tissue homogenate, the tissue can be fully cracked, the obtained chromatin amount is large, the quality is high, and the quality of a peak graph of the subsequent ChIP-Seq experiments is high. The method has the advantages of simpleness and rapidity, can effectively prepare a tissue suspension, can satisfy the core requirement of the ChIP-Seq experiments, and provides the effective approach for developing the pig tissue epigenomics researches.