4results about How to "Improve Sequencing Accuracy" patented technology

The invention relates to the technical field of molecular diagnosis, in particular to an F8 gene mutation detection probe group and a kit based on PacBio liquid phase hybridization capture. The probe group comprises sequences as shown in SEQ ID NO. 1 to SEQ ID NO. 313; the F8 gene specific probe is designed, a PacBio sequencing platform is adopted, the type, position and breakpoint of F8 gene mutation can be more accurately determined, the risk of missed diagnosis and misdiagnosis is reduced, when complex variations such as inversion of intron 22 and inversion of intron 1 of the F8 gene are detected, the position of the inverted breakpoint can be clearly recognized through PacBio sequencing, the detection period is shortened, and the detection efficiency is improved. And a way is provided for clinical diagnosis of hemophilia A.

The present invention relates to a method for discriminating between live and dead microbes in a sample, by discriminating between transcriptionally-active and inert microbial nucleic acid sequences in the sample. In particular, the method according to the present invention is based on the comparison of levels of nucleotide substitution in a sample cultured in presence of an RNA-labelling agent. The present invention further relates to a diagnosis method of microbial infections in a subject; and to methods of assessing the risk of contamination of a sample, implementing the method for discriminating according to the present invention.

This invention discloses a CoolMPS antibody mutant that specifically binds to a blocking-modified cytosine deoxynucleotide and its applications. The invention provides an antibody (referred to as an antibody mutant or modified antibody) that binds to a target, obtained by mutating the variable region of the heavy chain of the antibody to be modified; the mutation is located at: amino acid residue 2 of CDR1; amino acid residue 2 of CDR2; amino acid residue 15 of FR1; amino acid residue 6 of FR3; amino acid residue 21 of FR3; amino acid residue 30 of FR3; amino acid residue 38 of FR3; the target is a blocking-modified cytosine deoxynucleotide. The antibody provided by this invention can be used in basic scientific research, biosensor technology, gene sequencing, and other fields, and has broad application prospects.

The invention relates to a single-chain connector, a method for detecting the tail end of a nucleic acid fragment, a kit and application of the single-chain connector. The single-chain connector disclosed by the invention can be simultaneously connected with the same-side tail end of a to-be-detected double-chain nucleic acid fragment, a protruding tail end sequence of double-chain nucleic acid can be efficiently and accurately captured, and the motif and position information of the tail end of the double-chain nucleic acid can be favorably and accurately identified through sequencing analysis.