35results about How to "Reduce reagent consumption" patented technology

The invention discloses an integrated microfluidic chip for screening medicine. The integrated microfluidic chip comprises a PDMS (polydimethylsiloxane) fluid channel layer and a glass layer. The PDMS fluid channel layer and the glass layer are bonded with each other by oxygen plasma to form a fluid channel unit which is of an irreversible structure; the fluid channel unit comprises a concentration gradient generation structural region, an array cell culture region and a buffer structural region. The invention further provides a method for applying the integrated microfluidic chip. The integrated microfluidic chip and the method have the advantages that medicine concentration gradient generation, chip cell culture, medicine stimulation on cells, result generation and detection are integrated on the chip, the buffer structural region can be opened and closed, accordingly, the concentration gradient generation structural region and the array cell culture region can independently work and integrally run, and various functions can be flexibly switched over; cell inoculation, stimulated, washing and dyeing operation procedures and the like can be simplified by the aid of the method, various experimental correlation parameters can be obtained by means of one-step running, and a novel technical platform and the novel method can be provided for medicine screening and cell-medicine research.

The invention provides a method for producing a vanadium oxide. The method comprises the following steps of: mixing vanadium slag with calcium oxide or limestone to form a mixed material; roasting the mixed material to obtain a calcified clinker; leaching the calcified clinker through 5 to 12wt% of sodium carbonate solution at 80 to 95 DEG C; separating the solid and the liquid after the leaching reaction, so as to obtain vanadium-containing liquid and residues; processing the vanadium-containing liquid in a desilicating manner, so that the silicon concentration in the vanadium-containing liquid is less than 0.1g/L; cooling the vanadium-containing leaching liquid to 10 to 20 DEG C; adding ammonium carbonate to the vanadium-containing leaching liquid; adjusting the molar ratio of NH4<+>/TV to 2.5 to 7 to precipitate ammonium metavanadate; filtering to obtain ammonium metavanadate and vanadium-precipitated wastewater; and processing ammonium metavanadate by oxidizing, roasting and deaminizing to prepare vanadium pentoxide or by reducing to prepare vanadium trioxide. By adopting the method for producing the vanadium oxide, the vanadium oxide can be prepared through general vanadium slag and high-calcium high-phosphorus vanadium slag in the premise that the environmental protection requirement is met, the agent consumption can be decreased, and the production cost is reduced.

Disclosed are methods relating to cell membrane fragments associated with microbeads, so that the characteristics of the cells the fragments originated from can be determined. The fragments can be oriented with what was the outer surface of the cell membrane facing outwardly, so that the antigens associated with the membrane can be contacted with ligands (including antibodies) to antigens in the membranes which would be accessible to antibodies in vivo. The system is useful, inter alia, for detection of panel reactive antibodies in donor serum, as well as detection of other cell membrane antigens; or quantitation of particular cell membrane antigens.

Methods and apparatus are described for the processing (for example washing, incubation, etc.) of particles in which the particles suspended in a first fluid are introduced under laminar flow conditions into at least one first microchamber or first region of the same, in which a second fluid is introduced under laminar flow conditions into at least one second region of the microchamber or of a second microchamber, in such a way as not to mix with the first fluid, and in which at least one field of force acting on the particles is activated in the microchamber(s), to provoke a shift of the particles alone in a predetermined direction and to transfer the same in suspension into the second fluid; an apparatus is preferably used including at least three microchambers n microchambers arranged in sequence with each other in one direction and each connected with the microchamber immediately before it and after it with two orifices offset from each other in a direction perpendicular to the direction of sequence of the microchambers.

An electrochemical sensor for measuring the amount of hydrogen sulphide or thiols in a fluid comprises an electrically conductive porous electrode within which are dispersed a precursor and a reaction solution which together with the hydrogen sulphide or thiols a redox reaction resulting in an electrical current dependent upon the amount of hydrogen sulphide or thiols in the fluid. The reaction solution may be initially provided in the pores of the porous electrode, or derived in use from the fluid itself.

The present invention relates to cell separation using microfabricated devices. In particular, the present invention provides methods and devices for separation of sperm from biological materials, such as other cells and molecular species, in a cell mixture in a microfabricated device through the use of electroosmotic flow, electrophoretic mobility, pressure gradient, differential adhesion, and/or combinations thereof.

The invention discloses a method for step-by-step extraction of copper anode mud silver separating residues and belongs to the technical field of copper smelting comprehensive recovery. By means of the method, chlorine salt is used for separating gold, silver and lead, barium is separated through carbonic acid conversion-hydrochloric acid leaching, and separation of lead, barium, gold and silver and efficient concentration of tin are effectively achieved; and environment-friendly and efficient recovery of metal such as Au, Ag, Pb, Sn and Ba in the silver separating residues is successfully achieved. Meanwhile, effective solution system circulation of chlorination solution circulation, carbonic acid solution circulation and HCl regeneration circulation is developed for a lead and barium separation system, and consumption of a reagent is greatly reduced. By means of the method, technology conditions are moderate, high temperatures, high acid and strong base are not needed, and the large-scale industry is better achieved; due to self circulation of different solution systems, outward draining of waste water is avoided, all components in the silver separating residues are recycled, solid waste is prevented from being generated, and environment friendliness is achieved.

The present invention relates to a mineral processing technology and method for refractory chalcopyrite ores, particularly to a mineral processing technology and method for the separation of chalcopyrite from multiple natural types of copper ores containing chalcopyrite, pyrrhotite, talc and serpentine, which belongs to the technical field of mineral processing. It's characterized by: conducting a two-stage grinding on the chalcopyrite ore, with each grinding stage followed by size grading, and treating ores in different size fractions separately, wherein coarse-grain ores are separated in the presence of xanthate, with calcium oxide and CMC controlling the pH and acting as depressor respectively, while fine-grained ores are subjected to rougher in the presence of kerosene, and subsequently subjected to cleaner in the presence of xanthate.

An analytical test element for blood analyses is provided having an application site and a microfluidic channel structure in fluid communication with the application site. The channel structure comprises at least first and second analytical channels for receiving first and second portions of a blood sample applied to the application site. The first analytical channel comprises a first analytical site to determine the total haemoglobin value (Hb) of the blood sample. The second analytical channel comprises a second analytical site to determine a glycohaemoglobin value (HbA1c) of the blood sample.

The invention discloses a sampling membrane and a sampler capable of enriching heavy metals in ambient air and an application method of the sampler. The sampling membrane comprises a nanometer fiber membrane and an air-permeable supporting padding arranged below the nanometer fiber membrane. The sampler comprises the sampling membrane and a sampling clamp; the sampling clamp is composed of an upper cone and a lower cylinder, wherein the upper surface of the cone is horizontal, the cylinder is composed of an upper cylinder and a rotary lower cylinder, the side surfaces of the upper cylinder and the lower cylinder are provided with uniformly distributed vertical ridges, the vertical ridges on the upper cylinder and the lower cylinder are aligned by rotating the lower cylinder, so fixation of the sampling membrane on the horizontal surface of the sampling clamp is realized. After heavy metals in air are acquired by using the sampler, a filter membrane is taken out and put into a centrifuge tube filled with a buffer solution, a solution is used for desorption of the heavy metals on the filter membrane, and after desorption, the solution is determined. According to the invention, detection sensitivity can be substantially improved, pre-treatment of a sample is easy and feasible, and nanometer fiber filter membranes with different functions are used for different detection objects, thereby improving absorption and acquisition efficiency.

The present invention relates to a process for the preparation of a compound of the general Formula (I), comprising: a) reacting a compound of the general Formula (II) with a compound of the Formula III R²COOH and a compound of the general Formula IV R³NC under such conditions that compound I is formed, wherein R¹ represents a substituted or unsubstituted alkyl, alkenyl, alkynyl, aromatic or non-aromatic, mono-, di- or tricyclic, or heterocyclic structure, and R² represents a substituted or unsubstituted alkyl, alkenyl, alkynyl, aromatic or non-aromatic, mono-, di- or tricyclic, or heterocyclic structure, and R³ represents a substituted or unsubstituted alkyl, alkenyl, or alkynyl structure. In further aspect the subject invention relates to the use of the obtained products as intermediates for various peptidomimetics, and preferably as a building block in a convergent synthesis of prolyl dipeptide structures.

With respect to molecular biology, medicine, and biotechnology, provided is a method related to the performance of Polymerase Chain Reaction. The method includes using the device for identification of nucleic acids containing a microchip with at least one reaction zone on its surface. The microchip contains a heat-conducting substrate made of aluminum while each reaction zone is separated from the heat-conducting substrate by a layer of the passivating material covalently bound to the surface of the heat-conducting substrate while over the layer of the passivating material one or several dried components of the polymerase chain reaction is placed. In this method the ratio of the aggregate thermal mass of the microchip to thermal conductance of the microchip substrate does not exceeding 0.04 s. The technical result is reduced duration of the analysis, higher reliability, accuracy, efficiency and cost-effectiveness of the analysis.

A smear preparation apparatus comprises a blood processing unit, a washing unit, a controller, an information obtaining unit. The controller changes the washing condition for washing after processing a blood sample to a second washing condition which is stronger than the first washing condition when abnormal blood cells occur in the blood sample.

In the invention, a fluid cell, used for sequencing reaction between DNA fragments and reagents, comprises: a reaction chamber, one inner side of which is fixed with multiple DNA fragments; a reagent inlet and a reagent outlet, which are separately located at each end of the other inner side of the reaction chamber and used for reagents flowing into and out from the reaction chamber. Multiple DNA fragments are fixed on the reaction chamber with the small capacity as short tag arrays in sequence and make the reaction go on at the condition of the reagents without diffusion barriers, which improves the contacts of reagents and DNA fragments so as to shorten the reaction time. At the same time, the demands to the concentration and dosage of reagents are low, which reduces the consumption of reagents. As the result, the sequencing cost cut down. Multiple DNA fragments are fixed on the fluid cell at the same time, which provides a way for parallel reaction of various fragments. The automatic high-through sequencing and fast sequencing reactions are achieved in the invention.

A test module for concentrating pathogens in a biological sample, the test module having an outer casing with a receptacle for receiving the sample, a dialysis device in fluid communication with the receptacle and configured to separate the pathogens from other constituents in the sample, and, a lab-on-a-chip (LOC) device being in fluid communication with the dialysis device and configured to analyze the pathogens.

The invention belongs to the technical field of preparation and application of active carbon fiber materials, and particularly relates to a viscose-based nitrogen-containing active carbon fiber material as well as a preparation method and application thereof. The mass content of nitrogen in the material is 4.27-7.43%, the specific surface area is 609-1159 cm < 2 >/g, and the total pore volume is 0.31-0.54 cm < 3 >/g. The viscose-based nitrogen-containing active carbon fiber material is large in specific surface area and high in microporosity, the used raw materials are easy to obtain, economical and environmentally friendly, carbonization-activation is completed in one step in preparation of the viscose-based nitrogen-containing active carbon fiber material, operation is simple, and time consumption and energy consumption are reduced. The viscose-based nitrogen-containing active carbon fiber material has good adsorbability to carbon dioxide, has excellent CO2/CH4 selectivity, and has a wide application prospect in the field of gas adsorption and separation.