Integrated microfluidic chip for screening medicine and method for applying integrated microfluidic chip

A microfluidic chip and drug technology, applied in biochemical equipment and methods, chemical instruments and methods, laboratory containers, etc., can solve problems such as insufficient flux, low detection and analysis sensitivity, and limited popularization and application. , to avoid mutual interference, reduce the number of cells and reagent consumption, and save the tedious operation.

Inactive Publication Date: 2015-11-25
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0002] Drug screening is the initial process and key step of new drug research. The purpose is to discover new drugs. However, for a long time, the shortcomings of high cost, long cycle, complicated process, insufficient throughput, and low success rate have restricted the screening o

Method used

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  • Integrated microfluidic chip for screening medicine and method for applying integrated microfluidic chip
  • Integrated microfluidic chip for screening medicine and method for applying integrated microfluidic chip
  • Integrated microfluidic chip for screening medicine and method for applying integrated microfluidic chip

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Embodiment Construction

[0038] The present invention will be further described in detail below in conjunction with specific examples.

[0039] see figure 1 , figure 2 , the present embodiment provides an integrated microfluidic chip for drug screening, which is composed of an upper PDMS fluid channel layer 1 and a lower glass layer 2 suitable for cell adherent growth; the PDMS fluid channel layer 1 and glass Layer 2 forms a fluid channel unit with an irreversible structure through oxygen plasma bonding.

[0040] The fluid channel unit includes: a concentration gradient generation structure area 3 located upstream of the chip and an arrayed cell culture area 4 located downstream of the chip; a buffer structure is provided between the concentration gradient generation structure area 3 and the arrayed cell culture area 4 Area 5, the buffer structure area 5 is composed of eight columns of buffer units 6. The concentration gradient generation structure area 3 , the arrayed cell culture area 4 , and th...

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Abstract

The invention discloses an integrated microfluidic chip for screening medicine. The integrated microfluidic chip comprises a PDMS (polydimethylsiloxane) fluid channel layer and a glass layer. The PDMS fluid channel layer and the glass layer are bonded with each other by oxygen plasma to form a fluid channel unit which is of an irreversible structure; the fluid channel unit comprises a concentration gradient generation structural region, an array cell culture region and a buffer structural region. The invention further provides a method for applying the integrated microfluidic chip. The integrated microfluidic chip and the method have the advantages that medicine concentration gradient generation, chip cell culture, medicine stimulation on cells, result generation and detection are integrated on the chip, the buffer structural region can be opened and closed, accordingly, the concentration gradient generation structural region and the array cell culture region can independently work and integrally run, and various functions can be flexibly switched over; cell inoculation, stimulated, washing and dyeing operation procedures and the like can be simplified by the aid of the method, various experimental correlation parameters can be obtained by means of one-step running, and a novel technical platform and the novel method can be provided for medicine screening and cell-medicine research.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, in particular to an integrated microfluidic chip for drug screening and an application method thereof. Background technique [0002] Drug screening is the initial process and key step of new drug research. The purpose is to discover new drugs. However, for a long time, the shortcomings of high cost, long cycle, complicated process, insufficient throughput, and low success rate have restricted the screening of modern drugs. and development. At present, high-throughput screening based on 96-well plates is suitable for preliminary screening of drugs due to its rapidity and high efficiency. It has been widely used by most international drug research institutions and has developed rapidly. It has become the main technical means of drug screening. one. However, this drug screening method based on conventional orifice plates has some limitations, such as large reagent consumption, cumbersom...

Claims

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Application Information

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IPC IPC(8): B01L3/00C12M3/00C12Q1/02
Inventor 孟宪生庞磊包丽娜孙佳琳包永睿王帅
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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