Single-molecule fluorescent gene sequencing optical system

An optical system and fluorescent gene technology, applied in fluorescence/phosphorescence, material analysis through optical means, material analysis, etc., can solve the problems of high equipment cost and sequencing cost, low accuracy, long sequencing read length, etc., and achieve improved The effect of sequencing accuracy and simplification of dependence on multiple image sensors
CN112646703AActive Publication Date: 2021-04-13CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
CHANGCHUN INST OF OPTICS FINE MECHANICS & PHYSICS CHINESE ACAD OF SCI
Publication Date
2021-04-13

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Abstract

The invention provides a single-molecule fluorescent gene sequencing optical system which is a novel single-molecule real-time sequencing technology based on frequency scanning. The single-molecule fluorescent gene sequencing optical system comprises a sequencing chip and a plurality of single-wavelength pulse laser groups for exciting fluorescent signals, wherein exciting light of the pulse laser groups sequentially irradiates the sequencing chip through a laser light path, and a gene sequence to be detected is sequentially subjected to frequency scanning; then, optical imaging is performed through a fluorescent light path; and sequencing is performed on four basic group phosphoric acid segments of nucleotide molecules modified with different fluorescent molecules. A pulse laser scanning illumination mode is used, so that the light damage of the laser light intensity on DNA polymerase by continuous illumination in the conventional three-generation single-molecule real-time sequencing can be effectively reduced; the pulse scanning time is selected to be shorter than the polymerization extension time, so that in the ATGC cyclic sequencing process, before the next basic group starts to be detected, the sequencing accuracy can be improved through repeated measurement on the previous basic group.
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Description

technical field

[0001] The invention relates to the technical field of gene sequencing, in particular to a single-molecule fluorescent gene sequencing optical system. Background technique

[0002] Gene sequencing technology refers to the identification of the base sequence of a specific DNA fragment, that is, the sequence arrangement of adenine (A), thymine (T), cytosine (C) and guanine (G). At present, the gene sequencing market is mainly dominated by next-generation sequencing technology, typical representatives of which are Illumina's low, medium and high sequencing platforms, and Thermal Fisher's Ion Torrent sequencing platform. Compared with the traditional first-generation sequencing method, the second-generation sequencing has the outstanding characteristics of high throughput and low cost. Although the accuracy is not as good as the traditional sequencing method, due to the analysis of massive data, information beyond the sequence itself can be obtained, so it is als...

Claims

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