Single-molecule fluorescent gene sequencing optical system

Abstract

The invention provides a single-molecule fluorescent gene sequencing optical system which is a novel single-molecule real-time sequencing technology based on frequency scanning. The single-molecule fluorescent gene sequencing optical system comprises a sequencing chip and a plurality of single-wavelength pulse laser groups for exciting fluorescent signals, wherein exciting light of the pulse laser groups sequentially irradiates the sequencing chip through a laser light path, and a gene sequence to be detected is sequentially subjected to frequency scanning; then, optical imaging is performed through a fluorescent light path; and sequencing is performed on four basic group phosphoric acid segments of nucleotide molecules modified with different fluorescent molecules. A pulse laser scanning illumination mode is used, so that the light damage of the laser light intensity on DNA polymerase by continuous illumination in the conventional three-generation single-molecule real-time sequencing can be effectively reduced; the pulse scanning time is selected to be shorter than the polymerization extension time, so that in the ATGC cyclic sequencing process, before the next basic group starts to be detected, the sequencing accuracy can be improved through repeated measurement on the previous basic group.

Description

technical field [0001] The invention relates to the technical field of gene sequencing, in particular to a single-molecule fluorescent gene sequencing optical system. Background technique [0002] Gene sequencing technology refers to the identification of the base sequence of a specific DNA fragment, that is, the sequence arrangement of adenine (A), thymine (T), cytosine (C) and guanine (G). At present, the gene sequencing market is mainly dominated by next-generation sequencing technology, typical representatives of which are Illumina's low, medium and high sequencing platforms, and Thermal Fisher's Ion Torrent sequencing platform. Compared with the traditional first-generation sequencing method, the second-generation sequencing has the outstanding characteristics of high throughput and low cost. Although the accuracy is not as good as the traditional sequencing method, due to the analysis of massive data, information beyond the sequence itself can be obtained, so it is als...

AI Technical Summary

Problems solved by technology

Due to the high detection requirements of the former, the cost of equipment and sequencing has always been very high, and the throughput is limited by the existing nanofabrication technology. The latter has a simple structure and long sequencing read length, but its accuracy is relatively low.

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