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Rear-earth upconverting luminescence intensity ratio-based luminescent dye concentration detection method

A technology of rare earth up-conversion and fluorescent dyes, which is applied in the field of determination of the concentration of fluorescent dyes, can solve the problems of large errors in biosensors and inability to perform accurate measurements, and achieve the effects of small light damage, improved detection sensitivity, and improved detection sensitivity

Active Publication Date: 2017-09-15
DALIAN NATIONALITIES UNIVERSITY
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Problems solved by technology

However, the upconversion luminescence of rare earth ions is often affected by factors such as host material, luminescence energy level, material scale, excitation conditions, external environment and instrument conditions, etc., resulting in the upconversion luminescence intensity of rare earth ions being affected by the above factors and cannot be accurately determined measurement, making biosensors based on rare earth upconversion luminescence intensities have large errors

Method used

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  • Rear-earth upconverting luminescence intensity ratio-based luminescent dye concentration detection method
  • Rear-earth upconverting luminescence intensity ratio-based luminescent dye concentration detection method
  • Rear-earth upconverting luminescence intensity ratio-based luminescent dye concentration detection method

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Embodiment 1

[0035] (1) Preparation of rare earth Er on glass substrate 3+ Ion-doped up-conversion luminescent materials

[0036] Er was prepared by spin-coating method 3+ Doped YbMoO 4 Composite thin film: First, the Ag layer and the ZnO array layer are sequentially prepared on the surface of the glass substrate by the magnetron sputtering method and the low-temperature aqueous solution method, and then the surface is spin-coated with erbium nitrate (Er(NO 3 ) 3 ·5H 2 O) and ytterbium nitrate (Yb(NO 3 ) 3 ·5H 2 (0) ethanol solution, then spin-coat one deck of ammonium heptamolybdate ((NH 4 ) 6 Mo 7 o 24 4H 2 O) aqueous solution, wherein the pH value of ammonium heptamolybdate aqueous solution is adjusted to 7 by 1M NaOH solution. The sample obtained after spin-coating is placed in a muffle furnace, raised to 500°C at a rate of 3°C / min in an air atmosphere, and kept at this temperature for 1 hour. After the heat preservation is completed and cooled with the furnace, Er 3+ Dope...

Embodiment 2

[0040] Rare earth Tm was first prepared on a glass substrate 3+ Ion-doped up-conversion luminescent material, and then the film is attached to one side of a cuvette, which is filled with different concentrations of FITC aqueous solution. Tm was excited by 980nm laser 3+ Doped up-conversion luminescent film, on the other side of the cuvette, the Tm of the FITC solution was measured by a spectrometer 3+ The upconversion luminescence spectrum ( figure 2 ). By measuring the corresponding Tm 3+ of 1 D. 2 → 3 f 4 and 1 G 4 → 3 h 6 The ratio of the two blue upconversion luminescence intensities of the transition is established to establish the relationship with the FITC concentration, and the FITC detection of the fluorescence intensity ratio is realized.

[0041] From figure 1 and figure 2 The rare earth Er of the shown embodiment 1 and embodiment 2 3+ / Tm 3+ The upconversion luminescence spectrum of the doped film and the absorption spectrum of the fluorescent dye...

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Abstract

The invention relates to a rear-earth upconverting luminescence intensity ratio-based luminescent dye concentration detection method, in particular to a method for measuring the concentration of a luminescent dye by means of a rear earth ion upconverting luminescence intensity ratio. The method has the advantages of simple operation, high detection sensitivity and reusability. According to the method, proper rear earth ions capable of realizing effective radiative energy transfer with fluorescent dye molecules are selected according to a fluorescent dye type needing to be detected; the luminescent dye concentration can be detected by measuring rear-earth ion upconverting luminescence, which has two different wavelengths and is related to the luminescent dye concentration, and taking a intensity ratio of the rear-earth ion upconverting luminescence as a characterization parameter.

Description

technical field [0001] The invention relates to a method for measuring the concentration of fluorescent dyes, in particular to a technique based on the radiant energy transfer process of rare earth ions upconverting the luminous intensity ratio, which can be used for the detection of fluorescent dyes with energy transfer with rare earth ions. Background technique [0002] In recent years, due to the sharp emission, large Stokes shift and long fluorescence lifetime of rare earth ion luminescence, rare earth doped luminescent materials have attracted great interest in the fields of lighting, visualization applications, optical communication and biomedicine. Rare-earth-doped upconversion luminescent materials are widely used in biomedicine, photocatalysis, solar cells, and optical sensing due to the ability to convert low-energy photons into high-energy photons through a multiphoton absorption process. Among them, rare earth up-conversion luminescence also has the characteristi...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6402G01N2021/6421
Inventor 曹保胜吴金磊董斌何洋洋冯志庆
Owner DALIAN NATIONALITIES UNIVERSITY
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