The invention belongs to the technical field of ship
ballast water microalgae content detection, and particularly relates to an up-conversion nano fluorescent probe which realizes
rapid detection of microalgae content through competitive emission. According to the method, a red up-conversion nano fluorescent probe is adopted, red
luminescence of the fluorescent probe is highly overlapped with the maximum absorption peak of
ballast water microalgae
chlorophyll a under excitation of near-
infrared light, a relation curve of
luminescence intensity and the content of
chlorophyll a is established through competitive
luminescence measurement, and the content of the
chlorophyll a is determined according to the corresponding relation of the content of the
chlorophyll a and the microalgae
cell biomass. And establishing a relation model between the
luminous intensity and the microalgae
cell biomass so as to calculate the microalgae
biomass in the
ballast water sample. According to the present invention, the
rapid detection of the microalgae biomass can be achieved, the operation is simple and convenient, the sensitivity is high, the safe
long wavelength near-
infrared light is adopted as the excitation source, the
light damage to the
organism is reduced, the spontaneous
background fluorescence of the microalgae cannot be caused, the detection sensitivity can be improved in the
order of magnitude, and the method is suitable for promotion and application.