116results about How to "Light damage is small" patented technology

The invention discloses an inclined wide-field optical section scanning imaging microscope system and an imaging method thereof. The inclined wide-field optical section scanning imaging microscope system comprises a laser transmitting device, wherein laser transmitted by the laser transmitting device enters a laser scanning optical path consisting of a two-dimensional scanning galvanometer, a collimator lens group and a first microscope objective to perform inclined scanning on a sample on a sample platform; a second microscope objective, a field lens and a detector form an imaging detection optical path the optical axis of which is perpendicular to the laser scanning optical path; an imaging display control device respectively controls the synchronized action of the two-dimensional scanning galvanometer, the second microscope objective and the detector and the automatic displacement of the sample platform through a data acquisition card and a sample platform control device, and processes acquired imaging data of the detector to form wide field three-dimensional image information of the sample. The imaging microscope system and the imaging method disclosed by the invention have high scanning imaging speed and high resolution and can implement wide field scanning imaging.

The invention relates to a system and a method for realizing total internal reflection fluorescence microscopy by using a concentric double conical surface lens. The system comprises a parallel light generating device, and an annular light beam generating device, a fluorescence excitation device and an imaging device which are arranged on a light path in turn, wherein the parallel light generating device generates parallel light, and the annular light beam generating device is arranged on the path of the parallel light; and the annular light beam generating device comprises a hollow reflector which is arranged at a 45-degree angle to the incident direction of the parallel light, a concave surface conical lens arranged in the same axial with the parallel light, and a convex surface conical lens which is arranged in the center of the concave surface conical lens. The system and the method solve the low-transmittance of the conventional objective lens type total internal reflection fluorescence microscopy, have the advantage of an optical energy utilization rate as high as nearly 100 percent, and can realize the switching between total internal reflection fluorescence microscopy and common wide field fluorescence microscopy conveniently. The system and the method can image a single cell or a whole organelle and meet the requirements of most in-vivo biological experiments.

The invention discloses a neonatal jaundice therapeutic equipment optical device based on an LED light source. The neonatal jaundice therapeutic equipment optical device is characterized by comprising an LED light source module, collimating lenses and fly eye lens arrays; a plurality of LED light emitting chips are arranged on the LED light source module, the collimating lens is packaged in the front of each LED light emitting chip, the multiple LED light emitting chips are controlled by a plurality of control electrodes equivalently, and the fly eye lens arrays are arranged in front of the collimating lenses. Uniform and efficient lighting of a target lighting target surface and efficient therapy are achieved, and compared with an ordinary light source direct illuminating type mode, the neonatal jaundice therapeutic equipment optical device has the advantages that illuminating uniformity is greatly improved, light damage caused by too high local light intensity is reduced, and the therapeutic effect is improved.

The invention discloses a synthetic method and application of a porphyrin type near-infrared sulfur ion fluorescence probe. The fluorescence probe is prepared by reaction of TPP-OH and 2,4-dinitrobenzene sulfonyl chloride as raw materials, N,N-DIEA as a catalyst and dichloromethane as a solvent at room temperature. The probe can carry out fluorescence detection of high sensitivity and selectivity on sulfur ions. Compared with an existing small organic molecule fluorescence probe, the obtained fluorescence probe has longer fluorescence emission wavelength (lambda em > 650 nm), can carry out detection of high sensitivity, selectivity and timeliness on the micro sulfur ions on a near infrared region, and has huge application prospects in the biological and environment detection field.

The invention discloses a green quantum dot film and a preparation method of a backlight module thereof. The method performs functional design on the protective layer of the green quantum dot film; the outer surface of the protective layer has a good light diffusion effect after being coated, and inorganic diffusion particles in the coated layer play a role in blocking light in some bands, thus the light damage caused by long-time light irradiation on the quantum dot is slowed down. The invention also provides a using method of the green quantum dot film, the using method matches the green quantum dot film and a blue chip with red phosphor to make a novel LED light source, and the using method improves the stability of the quantum dot film while greatly increasing the color gamut coverageof the LCD.

The invention provides a method and a device of fluorescence microscopy by using a pyramid lens to generate structured lighting. The device comprises parallel beams, a structured lighting system, a sample cell and an image acquisition system, wherein the structured lighting system comprises a pyramid lens, a telescope system and a phase-shifting glass sheet; and the sample cell is arranged at the rear part of the phase-shifting glass sheet. The parallel beams are refracted after passing through the pyramid lens, thus generating a four-beam interference field with two-dimensional space structure distribution and exciting a sample by the four-beam interference field. The three-dimensional tomography can be realized through image processing algorithm by translating the interference optical field acted on the sample Compared with the existing microscopy for structured lighting, the invention can achieve higher axial resolution but lower photo-bleaching effect. Therefore, the invention is suitable for the research on the imaging of living creatures.

The invention relates to sunscreen compositions for humans including naturally occurring sunscreen agents from plants, algae, cyanobacteria, fungi and bacteria that protect against exposure to solar radiation. The active sunscreen agents are compounds that naturally occur in plants, algae, cyanobacteria, fungi and bacteria and derivatives of these compounds.

The invention discloses a lysosome-targeted hypochlorous acid near-infrared fluorescent probe as well as a preparation method and application thereof, which belongs to the technical field of analysischemistry. The lysosome-targeted hypochlorous acid near-infrared fluorescent probe is characterized in that a structural formula of the lysosome-targeted hypochlorous acid near-infrared fluorescent probe is as follows: (shown in the description). The invention also discloses a preparation method of the lysosome-targeted hypochlorous acid near-infrared fluorescent probe as well as application in water environment or biological cell system detection. The fluorescent probe of the invention has the characteristics of near-infrared transmission, high sensitivity, good selectivity, rapidness in response and the like.

The invention discloses an FRET (Fluorescence Resonance Energy Transfer) efficiency quantitative detecting method based on partial acceptor photo-bleaching and donor-acceptor alternate excitation. The invention relates to a quantitative detecting method, and particularly relates to the FRET efficiency quantitative detecting method based on the partial acceptor photo-bleaching and the donor-acceptor alternate excitation. According to the FRET efficiency quantitative detecting method, FRET efficiency of a donor-acceptor pair can be quantitatively obtained by the steps of partially photo-bleaching an acceptor, alternately exciting a sample by using donor exciting light and acceptor exciting light, and collecting fluorescence intensity of a donor channel and an acceptor channel before and after the acceptor is partially photo-bleached. The method directly detects the sample to be detected without any auxiliary sample or extra system correction and compensation. Not only can the FRET efficiency be quantitatively detected when the concentration ratio of the donor to the acceptor is 1 to 1, but also the FRET efficiency quantitative detection can be effectively realized when one donor and a plurality of acceptors exist. The FRET efficiency quantitative detecting method is simple in operation and causes small optical damage to the sample, can be used for repeatedly detecting the same sample, and can be applied to the rapid quantitative detection of the FRET efficiency of the donor-acceptor pair in the sample.

The invention discloses a preparation method and fluorescence detection application of a phosphorescence iridium complex. The phosphorescence complex has a chemical formula [Ir(CHO-btiq)2(bpy)][PF6]. The invention provides the preparation method of the phosphorescence iridium complex, and the application of the phosphorescence iridium complex to the detection of cysteine and homocysteine. The emission spectrum of the phosphorescent iridium complex is in the near-infrared region; biological imaging of the phosphorescent iridium complex has the advantages of little damage small, strong penetrability and little background spontaneous fluorescence; and the phosphorescent iridium complex has good application prospect in small animal in vivo fluorescence imaging. The iridium complex provided by the invention can realize high selectivity detection of cysteine and homocysteine, and provides the possibility for the construction of a simple and high-selectivity fluorescence chemical sensor for determination of cysteine and homocysteine.

The invention provides a copper cluster two-photon absorbing material with a living cell developing function, which is a univalent copper cluster with a multi-branched general chemical formula. The copper cluster two-photon absorbing material is prepared by the steps of: firstly, preparing a pyridine ligand from 4-methylpyridine and 4-N,N-2R-aminobenzaldehyde; and secondly, synthesizing a target product by using the pyridine ligand and cuprous iodide.

The invention provides a two-photon red-light-emitting fluorescent probe for detecting mitochondria and application of the same to cell imaging. The chemical name of the probe is 2-(pyrenyl-1-vinyl)-N-hexyl-quinoline iodate. The fluorescent probe can be synthesized through the following steps: heating 2-methylquinoline and iodohexane in an ethanol solution to synthesize 1,2-dimethylquinoline iodate at first; and then with tetrahydropyrrole as a catalyst, adding pyrenyl-1-carbaldehyde, and carrying out a condensation reaction at room temperature to produce 2-(pyrenyl-1-vinyl)-N-hexyl-quinolineiodate. The invention also provides the application of the fluorescent probe to imaging of mitochondria. The probe is simple to synthesize, can emit red light in single-photon and two-photon microscopes and image mitochondria, and has great application prospects.

The invention relates to the technical field of organic synthesis and novel materials and particularly relates to a near-infrared fluorescent compound with AIE performance as well as a preparation method and application thereof. According to the fluorescent compound, indole salts are used as receptors; a pyrrole donor and donors introduced with different conjugated structures in a site 2 and a site 5 of pyrrole are used for effectively regulating and controlling fluorescence emission of molecules; groups in a site 1, the site 2 and the site 5 are used for forming certain distortion with a pyrrole ring, so that the AIE performance is given to the compound; because of D-Pi-A actions in the molecules, the molecules can achieve long-wave-band light emission; the light emission wavelength is between 600 and 750nm and has greater Stokes shift. According to the fluorescent compound, the indole salts are used as targeting groups; the fluorescent compound is capable of achieving targeting imaging of mitochondria in various cells, has the advantages of washing-free property, low biotoxicity and high photobleaching resistance and the like in the imaging process, and is also capable of monitoring dyeing of the mitochondria in real time.

The invention discloses a long wavelength type thiophenol fluorescent probe as well as a preparation method and application thereof. According to the long wavelength type thiophenol fluorescent probe, amino porphyrin and 2,4-dinitrobenzenesulfonyl chloride are used as raw materials and proper reaction conditions are controlled to obtain a target probe compound. The fluorescent probe disclosed by the invention has a very long fluorescence emission wavelength (lambda-em is more than 650nm), rapid response and high anti-interference capability; high-selectivity and high-sensitivity fluorescent recognition and detection on trace thiophenol in a water environment and a biological sample can be realized; the long wavelength type thiophenol fluorescent probe has very good application prospect.

The invention provides a single-molecule fluorescent gene sequencing optical system which is a novel single-molecule real-time sequencing technology based on frequency scanning. The single-molecule fluorescent gene sequencing optical system comprises a sequencing chip and a plurality of single-wavelength pulse laser groups for exciting fluorescent signals, wherein exciting light of the pulse laser groups sequentially irradiates the sequencing chip through a laser light path, and a gene sequence to be detected is sequentially subjected to frequency scanning; then, optical imaging is performed through a fluorescent light path; and sequencing is performed on four basic group phosphoric acid segments of nucleotide molecules modified with different fluorescent molecules. A pulse laser scanning illumination mode is used, so that the light damage of the laser light intensity on DNA polymerase by continuous illumination in the conventional three-generation single-molecule real-time sequencing can be effectively reduced; the pulse scanning time is selected to be shorter than the polymerization extension time, so that in the ATGC cyclic sequencing process, before the next basic group starts to be detected, the sequencing accuracy can be improved through repeated measurement on the previous basic group.

A microscopy device comprises a continuous or pulsed wave laser light source; a pair of parallel mirrors configured to receive light from the light source and reflect an array of incoherent light sheets; a beam encoder (e.g., frequency modulation reticle, Hadamard basis, random modulation pattern) to segment the array of incoherent light sheets and encode each light sheet with a respective frequency in reciprocal space; a lens configured to direct the encoded light sheets towards a biological sample; and an image capturing device configured to receive a fluorescence signal from the biological sample.

The invention discloses a prezatide copper acetate essence and a preparation method thereof. The prezatide copper acetate essence comprise the following raw materials: water, glycerol, hydrolyzed collagen, microalgae parvum polysaccharide, maltooligosaccharide glucoside, hydrogenated starch hydrolysate, tripeptide-1 copper, acetyl hexapeptide-8, dipeptide-1, dipeptide-2, sodium hyaluronate, trehalose, a cactus tangutorum stem extract, a zanthoxylum bungeanum fruit extract, 1,2-pentanediol, a pomelo extract, a witchhazel extract, arginine and imidazolidinyl urea. The prezatide copper acetate essence is safe and healthy, and has effects of wrinkle resistance, aging resistance, firmness, moistening, oxidation resistance, whitening, repair regeneration, red luster and immune defense when usedon skin. The loose skin can be tightened, so that the elasticity of the skin is improved; and photodamage is relieved, and spots are removed.

The invention discloses a mitochondrial pH fluorescent probe based on benzothiazole, and a preparation method and an application thereof. The probe is specifically named as 2-(2-(6-hydroxynaphthalene-2-yl)vinyl)-3-(6-(triphenylphosphonyl)hexyl)benzothiazole-3-bromide (HTBT2). The preparation method comprises the steps: firstly, carrying out a reaction of 2-methylbenzothiazole and 1,6-dibromohexaneunder heating conditions to prepare 3-(6-bromohexyl)-2-methylbenzothiazole-3-bromide (BMBI), and then carrying out mixed reflux of the prepared BMBI with triphenylphosphine and acetonitrile to prepare 2-methyl-3-(6-(triphenylphosphorus)hexyl)benzothiazole-3-bromide (MTBI); and finally, dissolving MTBI and 6-hydroxy-2-naphthaldehyde in ethanol, and adding a small amount of piperidine, refluxing, and then separating and purifying to obtain HTBT2. The probe has the pKa value of 8.04+ / -0.02 and is quite close to that of a mitochondrial matrix (with the pH of 8.0). At the same time, the probe hasthe advantages of high sensitivity to the changes of the pH, good selectivity and large Stokes displacement, and can be used for monitoring the changes of the pH in cell mitochondria.

The invention belongs to the technical field of ship ballast water microalgae content detection, and particularly relates to an up-conversion nano fluorescent probe which realizes rapid detection of microalgae content through competitive emission. According to the method, a red up-conversion nano fluorescent probe is adopted, red luminescence of the fluorescent probe is highly overlapped with the maximum absorption peak of ballast water microalgae chlorophyll a under excitation of near-infrared light, a relation curve of luminescence intensity and the content of chlorophyll a is established through competitive luminescence measurement, and the content of the chlorophyll a is determined according to the corresponding relation of the content of the chlorophyll a and the microalgae cell biomass. And establishing a relation model between the luminous intensity and the microalgae cell biomass so as to calculate the microalgae biomass in the ballast water sample. According to the present invention, the rapid detection of the microalgae biomass can be achieved, the operation is simple and convenient, the sensitivity is high, the safe long wavelength near-infrared light is adopted as the excitation source, the light damage to the organism is reduced, the spontaneous background fluorescence of the microalgae cannot be caused, the detection sensitivity can be improved in the order of magnitude, and the method is suitable for promotion and application.

The invention discloses a near-infrared fluorescent probe responding to peroxynitroso anions, and a preparation method and an application thereof. The structure of the near-infrared fluorescent probeis shown in the description. The preparation method has the advantages of easiness in obtaining of raw materials, mild and easily-controlled reaction conditions, and saving of the reaction cost, and the obtained near-infrared fluorescent probe responding to peroxynitroso anions has a high selectivity and can be applied to a Parkinson's disease model.

The invention provides a femtosecond time-resolved multi-channel lock-in fluorescence spectrometer based on optical parametric amplification. The spectrometer includes a laser light source and a light beam splitting sheet; a sample excitation light generating part for frequency conversion of fundamental frequency light outputted by the laser light source; a sample excitation light focusing device and a sample-fixing sample pool; a sample fluorescence collecting and condensing system; a generating part of pumping light required by fluorescence optical parametric amplification; an optical parametric crystal for allowing the pumping light and fluorescence to generate a non-collinear optical parametric process; a time-resolved fluorescence spectrum data acquisition system; and a light path delay system for changing time delay of the optical parametric pumping light and the sample excitation light. The data acquisition system is a data acquisition system based on a multi-channel lock-in amplifier. Time-resolved fluorescence spectra without super fluorescent background interference can be obtained by single measurement.

The invention discloses a near-infrared absorption water-soluble conjugated polymer phototherapy reagent and preparation and application thereof. Fluorene derivatives are used as electron donors (D);pyrrolopyrroledione derivatives are used as electron acceptors (A), olefinic bonds are [pi]-conjugated bridges, an organometallic catalyzed Heck reaction is adopted for alternate copolymerization, a near-infrared absorption neutral conjugated polymer with a conjugated main chain being D-[pi]-A is obtained, and then the near-infrared absorption cationic water-soluble conjugated polymer is obtainedthrough a quaternization reaction. The absorption wavelength of the water-soluble conjugated polymer is in a biological optical window, and the water-soluble conjugated polymer has good water solubility and biocompatibility, excellent photothermal conversion ability and singlet oxygen yield, can effectively inhibit the growth of tumor cells, and is an excellent phototherapy reagent.

The invention provides a high-sensitivity near-infrared fluorescent compound capable of being used for detecting viscosity as well as a preparation method and application thereof. The structure of the near-infrared fluorescent compound is represented by a formula (I). The compound disclosed by the invention has the main beneficial effects that the compound (I) can be used as a fluorescent probe for detecting viscosity, the fluorescence property excitation of the compound is that ex is equal to 480 nm and em is equal to 670 nm, and the compound has relatively large Stokes shift, has the advantages of low background interference, small light damage to a biological sample and the like and is high in viscosity sensitivity; and an effective research tool is provided for researching the physiological action of viscosity in cells.

The invention discloses a near infrared fluorescence probe for monitoring lysosomal pH as well as preparation method and application thereof, belonging to the technical field of analytical chemistry.The near infrared fluorescence probe is characterized by having the following structural formula: (shown in the specification). The invention further discloses the preparation method of the near infrared fluorescence probe for monitoring lysosomal pH and the application thereof in selective detection of pH in water environments and biological cell systems. The near infrared fluorescence probe provided by the invention had the advantages of near infrared region emission, good selectivity, good light stability, good reversibility, and excellent lysosome targeting capacity.

The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.

The invention discloses a near-infrared fluorescent probe for bimodal detection of sulfur dioxide, and a preparation method and application thereof. The near-infrared fluorescent probe for bimodal detection of sulfur dioxide is a hemicyanine dye derivative, and the structure of the near-infrared fluorescent probe is shown in the specification. The fluorescent probe can be used for bimodal detection of sulfur dioxide in a water body by a colorimetric method and a ratio method, and after the sulfur dioxide reacts with the probe, two signals of probe solution color (dark blue-light powder) and fluorescence (near infrared-yellow region) are remarkably changed, so that high-selectivity and high-sensitivity dual visual detection of the sulfur dioxide in the water body is realized. The fluorescent probe provided by the invention can realize dual-channel ratio imaging and quantification of sulfur dioxide in living cells, and long-wavelength absorption and near-infrared emission of the probe can effectively avoid interference of biological background fluorescence and reduce light damage to the cells.

The invention provides a miniaturized optoisolator with bandwidth of 1 mu m. The miniaturized optoisolator with the bandwidth of 1 mu m is suitable for serving as an optoisolator in high-power lasers used for laser processing and the like, such as a fiber laser. The miniaturized optoisolator with the bandwidth of 1 mu m comprises a Faraday rotor, a first hollow magnet, a second hollow magnet unit and a third hollow magnet unit, wherein the Verdet constant of the Faraday rotor under the wavelength of 1.06 mu m is over 0.27min / (Oe*cm); the first hollow magnet is arranged on the periphery of the Faraday rotor; and the second hollow magnet unit and the third hollow magnet unit are arranged on an optical axis to clamp the first hollow magnet in the middle. The miniaturized optoisolator with the bandwidth of 1 mu m is characterized in that the second hollow magnet unit and the third hollow magnet unit are formed by more than two magnets formed by equal cutting along the direction which forms a 90-degree angle with the optical axis direction, the magnetic flux density B(Oe) applied to the Faraday rotor is within the range of a formula (1) which is that 0.5*104<=B<=1.5*104; and the optical path length L (cm) of the Faraday rotor is within the range of a formula (2) which is that 0.7<=L<=1.10.

The invention relates to a high-speed WFOV (wide field of view) CARS (coherent anti-stokes raman scattering) microscope system and a high-speed WFOV CARS microscope method. In the invention, pumping laser and Stokes light laser which are totally coincident in the aspects of space and time are subjected to weak convergence, so that a sample generates a CARS signal; and the CARS signal enters a CCD (Charge Coupled Device) camera through an optical filter and a cylindrical lens so as to obtain a clear CARS image. The invention utilizes the CARS signal to image and relates to an imaging technology based on the vibration characteristic of energy level inside molecules. The high-speed WFOV CARS microscope system and the high-speed WFOV CARS microscope method can be used for detecting chemical compositions of the sample and can be used for carrying out imaging on a single cell, even a single organelle. The requirements of most of biological experiments are totally met. The technical problems of low imaging speed, series photic damage to living biological tissues and the like of the existing CARS microtechnique are solved. Compared with the common fluorescence microscopy, the high-speed WFOV CARS microscope system and the high-speed WFOV CARS microscope method have the advantages that an external fluorescent probe does not need to be used, and influence on the molecular structure of the sample cannot be caused.

The invention relates to a rear-earth upconverting luminescence intensity ratio-based luminescent dye concentration detection method, in particular to a method for measuring the concentration of a luminescent dye by means of a rear earth ion upconverting luminescence intensity ratio. The method has the advantages of simple operation, high detection sensitivity and reusability. According to the method, proper rear earth ions capable of realizing effective radiative energy transfer with fluorescent dye molecules are selected according to a fluorescent dye type needing to be detected; the luminescent dye concentration can be detected by measuring rear-earth ion upconverting luminescence, which has two different wavelengths and is related to the luminescent dye concentration, and taking a intensity ratio of the rear-earth ion upconverting luminescence as a characterization parameter.