Massively parallel sequencing using unlabeled nucleotides

An unlabeled, deoxyribonucleotide technology, applied in the direction of microbial determination/inspection, biochemical equipment and methods, can solve the problems of signal reduction and low incorporation efficiency
CN113272448APending Publication Date: 2021-08-17MGI TECH CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
MGI TECH CO LTD
Publication Date
2021-08-17

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Abstract

The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.
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Description

technical field

[0001] The present invention relates to nucleic acid sequencing and its use in medicine and biological sciences.

[0002] sequence listing

[0003] This application contains a Sequence Listing, which has been filed electronically in ASCII format, and is hereby incorporated by reference in its entirety.

[0004] Cross References to Related Applications

[0005] This application claims U.S. Provisional Patent Application 62 / 758,317, filed November 9, 2018, U.S. Provisional Patent Application 62 / 914,877, filed October 14, 2019, U.S. Provisional Patent Application 62 / 914,940, filed October 14, 2019 , and the priority of U.S. Provisional Patent Application 62 / 914,915, filed October 14, 2019, each of which is incorporated herein by reference.

[0006] This application is related to United States Patent Publication US 2018 / 0223358 and International Patent Application PCT / US2018 / 012425 published as WO 2018 / 129214, both of which are incorporated herein by reference. ...

Claims

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