Femtosecond time-resolved multi-channel lock-in fluorescence spectrometer based on optical parametric amplification

A technology of optical parameter amplification and fluorescence spectrometer, which is applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of hyperfluorescence spectrum difference, hyperfluorescence spectrum intensity difference, and inability to accurately deduct hyperfluorescence background, etc., to achieve compact structure, The effect of reducing sample photodamage, improving dynamic range and detection sensitivity
CN104048948AActive Publication Date: 2014-09-17INST OF PHYSICS - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN ยท China
Current Assignee / Owner
INST OF PHYSICS - CHINESE ACAD OF SCI
Publication Date
2014-09-17

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Abstract

The invention provides a femtosecond time-resolved multi-channel lock-in fluorescence spectrometer based on optical parametric amplification. The spectrometer includes a laser light source and a light beam splitting sheet; a sample excitation light generating part for frequency conversion of fundamental frequency light outputted by the laser light source; a sample excitation light focusing device and a sample-fixing sample pool; a sample fluorescence collecting and condensing system; a generating part of pumping light required by fluorescence optical parametric amplification; an optical parametric crystal for allowing the pumping light and fluorescence to generate a non-collinear optical parametric process; a time-resolved fluorescence spectrum data acquisition system; and a light path delay system for changing time delay of the optical parametric pumping light and the sample excitation light. The data acquisition system is a data acquisition system based on a multi-channel lock-in amplifier. Time-resolved fluorescence spectra without super fluorescent background interference can be obtained by single measurement.
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Description

technical field

[0001] The invention belongs to the technical field of femtosecond time-resolved fluorescence spectrum measurement. Specifically, the invention relates to a femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parameter amplification. Background technique

[0002] Time-resolved fluorescence spectroscopy is an important technical method in the fields of photophysics, photochemistry, and biology. It is used to obtain information such as excited state lifetime, energy or electron transfer, and structural changes in the system. The current methods for realizing femtosecond time-resolved fluorescence spectroscopy include fluorescence up-conversion technology, optical Kerman technology, and femtosecond time-resolved fluorescence non-collinear optical parametric amplification spectroscopy technology. Compared with the former two, femtosecond time-resolved fluorescence non-collinear optical parametric amplification spectrosc...

Claims

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