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Femtosecond time-resolved multi-channel lock-in fluorescence spectrometer based on optical parametric amplification

A technology of optical parameter amplification and fluorescence spectrometer, which is applied in the direction of fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of hyperfluorescence spectrum difference, hyperfluorescence spectrum intensity difference, and inability to accurately deduct hyperfluorescence background, etc., to achieve compact structure, The effect of reducing sample photodamage, improving dynamic range and detection sensitivity

Active Publication Date: 2014-09-17
INST OF PHYSICS - CHINESE ACAD OF SCI
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Problems solved by technology

However, there are differences in the shape of the hyperfluorescence spectrum between adjacent optical parametric pump light pulses, and only when the energy amplification of the fluorescence photon satisfies the small signal approximation, the energy amplification of the fluorescence photon will not cause the change of the hyperfluorescence spectrum shape
Second, the intensity of the hyperfluorescence spectrum recorded alone differs from that recorded in the presence of fluorescence photon energy amplification
Therefore, the above method of step-by-step measurement cannot accurately subtract the interference of the hyperfluorescent background on the time-resolved fluorescence spectrum

Method used

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  • Femtosecond time-resolved multi-channel lock-in fluorescence spectrometer based on optical parametric amplification
  • Femtosecond time-resolved multi-channel lock-in fluorescence spectrometer based on optical parametric amplification
  • Femtosecond time-resolved multi-channel lock-in fluorescence spectrometer based on optical parametric amplification

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Embodiment 1

[0032] The present invention provides as attached figure 2 The shown femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parametric amplification mainly includes the following parts:

[0033] 1) The laser light source 201 is a titanium-doped sapphire femtosecond regeneration amplification system (central wavelength around 800nm, pulse repetition frequency 1kHz, single pulse energy greater than 300uJ, pulse width about 100fs).

[0034] 2) The light beam splitter 202 has a transmission and reflection ratio of 1:1 for the 800nm ​​beam, and is used to divide the laser output from the laser light source 1 into a transmitted beam and a reflected beam.

[0035] 3) The sample excitation light part is located in the optical path of the above-mentioned transmitted light beam, including a non-collinear optical parametric amplifier (NOPA) 203, a frequency doubler (SHG) 204 and an optical filter 205, wherein the non-collinear optical parametric ...

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Abstract

The invention provides a femtosecond time-resolved multi-channel lock-in fluorescence spectrometer based on optical parametric amplification. The spectrometer includes a laser light source and a light beam splitting sheet; a sample excitation light generating part for frequency conversion of fundamental frequency light outputted by the laser light source; a sample excitation light focusing device and a sample-fixing sample pool; a sample fluorescence collecting and condensing system; a generating part of pumping light required by fluorescence optical parametric amplification; an optical parametric crystal for allowing the pumping light and fluorescence to generate a non-collinear optical parametric process; a time-resolved fluorescence spectrum data acquisition system; and a light path delay system for changing time delay of the optical parametric pumping light and the sample excitation light. The data acquisition system is a data acquisition system based on a multi-channel lock-in amplifier. Time-resolved fluorescence spectra without super fluorescent background interference can be obtained by single measurement.

Description

technical field [0001] The invention belongs to the technical field of femtosecond time-resolved fluorescence spectrum measurement. Specifically, the invention relates to a femtosecond time-resolved multi-channel phase-locked fluorescence spectrometer based on optical parameter amplification. Background technique [0002] Time-resolved fluorescence spectroscopy is an important technical method in the fields of photophysics, photochemistry, and biology. It is used to obtain information such as excited state lifetime, energy or electron transfer, and structural changes in the system. The current methods for realizing femtosecond time-resolved fluorescence spectroscopy include fluorescence up-conversion technology, optical Kerman technology, and femtosecond time-resolved fluorescence non-collinear optical parametric amplification spectroscopy technology. Compared with the former two, femtosecond time-resolved fluorescence non-collinear optical parametric amplification spectrosc...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 翁羽翔党伟王专于清旭陈珂毛鹏程王云鹏
Owner INST OF PHYSICS - CHINESE ACAD OF SCI
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