51results about How to "The experiment takes a short time" patented technology

The invention discloses a core-shell structure catalyst for fuel cells and its pulse electrodeposition preparation method. The active component of the catalyst is a nanoparticle with a core-shell structure, and an active metal is cladded in the form of an ultrathin shell on the surface of a carbon carrier loaded metal or alloy nanoparticle serving as a core. The catalyst takes a non-platinum noble metal or transition metal as the core, and adopts more than one of Pt, Ir or Au as the shell. The preparation method includes: preparation of the nanoparticle serving as the core, making of a working electrode for pulse electrodeposition, and preparation of the catalyst by pulse electrodeposition. The catalyst can be used as an anode or cathode catalyst of a low temperature fuel cell. The obtained catalyst has very high stability. Compared with underpotential deposition, the method is simple to operate, has no need for inert atmosphere protection, and is more suitable for large-scale industrial production, also can greatly reduce the noble metal consumption of fuel cells, and greatly reduce the cost of fuel cells, thus having great significance in promoting the commercialization process of fuel cells.

The invention discloses an SSR molecular marker method of a brassica allohexaploid and primers thereof. The SSR molecular marker method of the brassica allohexaploid comprises the steps: hybridizing a brassica allohexaploid parent, and performing microspore culture on the obtained filial generation to obtain a DH colony; extracting genome DNAs of the brassica allohexaploid parent and the DH colony, and performing PCR amplification with all genome DNAs as a template by using the primers; constructing a genetic map of the brassica allohexaploid parent according to a PCR amplification result, wherein 217 pairs of primers are included, with base sequences respectively shown in SEQ ID No. 1/2-433/434. According to the SSR molecular marker method, a first genetic map of the brassica allohexaploid is constructed, and the basis is provided for further performing QTL location and molecular marker assistant breeding.

The invention relates to the field of aluminum alloy thermal treatment equipment and process stimulators, in particular to a thermal treatment production stimulating device and experimental method. The thermal treatment production stimulating device comprises a solid solution furnace, an aging oven and a cooling device, wherein the solid solution furnace is connected with the cooling device; and the aging oven is separately connected with the solid solution furnace and the cooling device. The thermal treatment production stimulating experimental method utilizes the device to perform simulation experiment, and comprises the following experimental steps of: S1, performing solid solution on an aluminum alloy material slice; S2, performing cooling or aging treatment on the aluminum alloy material slice subjected to solid solution; and S3, performing ageing treatment on the cooled aluminum alloy material slice. According to the thermal treatment production stimulating device and experimental method disclosed by the invention, various aluminum alloy sheet and strip thermal treatment key equipment such as solid solution equipment, cooling equipment and aging equipment are integrated onto one set of devices, so that experimental test is convenient, flexibility is strong, experimental cost is low, experiment can be repeatedly performed, experimental parameters can be precisely controlled, experimental repeatability is good, experimental time consumption is short and experiment can be performed at any time. While a great deal of investment is saved, cost and time of experiment are also saved.

The invention discloses a method for extracting bacterium genomic DNA by using magnetic nanoparticles, belonging to the technical field of nanomaterials and molecular biology. The method comprises the steps of: preparing a magnetic nanoparticle aggregate, preparing a bacterium lysing solution, preparing and adding an absorption buffer solution, absorbing with a magnet, rinsing with 70 percent alcohol, and eluting with a TE buffer solution to obtain a bacterium genomic DNA solution. Compared with the traditional phenol/chloroform DNA extracting method, the method for extracting the bacterium genomic DNA by using magnetic nanoparticles has the advantages of short experiment time, simpleness in operation, high extraction efficiency, rapidness, convenience, sensitivity, high yield and the like; and the extracted bacterium genomic DNA can be completely used for subsequent experiments, such as DNA hybridization, PCR (Polymerase Chain Reaction), and the like, and provides the basis for the subsequent research and analysis.

The invention provides a microstructure parameter-based clay consolidation coefficient prediction method, which comprises the steps of: conducting clay consolidation experiments under different pressures, and measuring the void ratio according to conventional geotechnical experiments; calculating the consolidation coefficients of different consolidation experiments; employing a scanning electron microscope technique to obtain a microstructure photo of a corresponding sample section; carrying out granule area and void area segmentation on the clay microstructure photo, and extracting the information of granule unit sum, unit direction, total area, total circumference, and area ratio; analyzing the granules and establishing a main component expression with an accumulative contribution rate of over 95%; and establishing the relationship between the main components and the clay consolidation coefficient. The method provided in the invention links the consolidation coefficient of clay with its microstructure parameters to obtain corresponding consolidation coefficient indexes under different pressures according to the clay void ratio and micro-parameter main components. The method can effectively shorten the experiment time at a civil engineering investigation phase.

The invention discloses a suspension device and a suspension method, and belongs to the technical field of animal experiments. The suspension device includes a tail tying belt, a suspension rope and an inflation mechanism; the tail typing belt includes a cloth bag and an air bag; the cloth bag includes a first side and a second side, the first side is opposite to the second side, and the first side is bonded to the second side; the air bag is arranged in the cloth bag; both ends of the suspension rope are respectively connected with the first side; and the inflation mechanism is communicated with the air bag. The suspension device and the suspension method can realize safe, fast and lossless suspension of an experimental animal.

The invention discloses a marine acoustic phase center calibration method, and relates to the field of marine surveying and mapping. The problems that according to an existing acoustic phase center calibration method, a pool experiment needs to be carried out in advance to calibrate the acoustic phase center of an acoustic array, the experiment time consumption is long, and the efficiency is low are solved. The method comprises the following steps: S1, enabling a workboat to sail around a seabed beacon according to a preset track, and collecting data; S2, calculating the installation position deviation DX of a seabed beacon coordinate matrix and an acoustic array in a geodetic coordinate system by using the data collected in S1; S3, calculating coordinates of each element on an acoustic array in the workboat coordinate system according to the sum DX; and S4, calculating the length of each base line according to the coordinate of each element on the acoustic array in the workboat coordinate system, and calibrating the acoustic phase center of each element through each base line length. The method is mainly used for calibrating the element acoustic phase center.

The invention discloses a method for extracting the total RNA of microalgae cells. The method has the advantages that microalgae is placed in a plant RNA extraction auxiliary fluid containing silica sand to be grinded, thereby achieving that grinding and polysaccharide removal treatment of the microalgae is rapidly completed through one step, greatly reducing the demands for the microalgae thallusamount, greatly simplifying the experimental operation, reducing the experimental time consumption. According to the method, breaking of the microalgae cell wall is combined with the removal of the microalgae so that the cell wall breaking and polysaccharide removal are completed within 5 min; through the innovation and improvement of the whole extraction method, the extraction time of the microalgae RNA is shortened to less than 30 min; on this basis, it is achieved that all the operations of the extraction of the microalgae RNA can be carried out in a general laboratory environment, and a special extraction environment of RNA enzyme removal is not needed; all experimental supplies do not need DEPC water for RNA enzyme removal treatment; the DEPC water is not harmful to the health of researchers while the early-stage preparation work is reduced, and the safety of experimenters is protected.

The invention relates to a method for evaluating the adherence strength of cells by utilizing a cell detachment agent. The method is technically characterized in that the adhesive effect between the cells and a substrate is weakened by using the cell detachment agent serving as a common experimental agent in cell biology, the cells are fixed later, the number of the adherent cells of each treatedconcentration gradient treatment group is detected, a detection value of a phosphate buffer solution (PBS) treatment group is used as a standard value and normalization analysis is performed, a curvegraph which takes NA as horizontal coordinates and takes D as vertical coordinates is drawn, and the adhesion strength of the cells is characterized by dilution multiples D50 percent corresponding to1/2NA(PBS). Compared with the prior art, the method has the advantages that time consumed in experiments is reduced, experimental steps are simplified, automated batch treatment is easy to realize, and the measuring accuracy and the parallelity of the results are improved.

The invention discloses a method for measuring the solid content of catalytic slurry oil, which comprises the steps of slurry oil heat treatment, solvent dilution, centrifugal treatment, filtration and enrichment, drying and weighing and the like, can accurately measure the solid content of the slurry oil, and can obtain a certain amount of solid fine powder sample for property characterization at the same time. According to the method, the problem of reading errors in calculation of the solid content volume by a traditional centrifugal method is solved, neglect of the ash content in measurement of the coke powder content is avoided, the time for measuring the solid content by a single filtering method is shortened, the measurement of the solid content of the oil slurry is more accurate, stable and rapid, meanwhile, a certain amount of solid content can be obtained for analysis of properties such as particle size and composition. Technical support is provided for investigation of oil slurry solid content and other properties and safe and stable operation monitoring of refinery plant devices.

The invention provides a mammal oocyte quality evaluation method based on optical property detection. The method comprises the following steps: oocytes are collected from a mammal, and the oocytes donot need any biochemical treatment; a Mueller matrix type full-field OCT system is combined with a Mueller matrix decomposition and transformation technology to represent the polarization characteristics of each biological structure of oocytes; a full-field OCT system is combined with a Fourier analysis technology to characterize backscattering spectrum characteristics of all biological structures of oocytes; and the backscattering intensity characteristics of each biological structure of the oocyte are characterized by analyzing the gray scale distribution characteristics and the frequency spectrum characteristics of the OCT image of the oocyte. The method has the characteristics of no damage, no labeling, high resolution, three-dimensional chromatography, simplicity in operation, shortexperimental time, easiness in implementation and the like, and can be used for effectively evaluating the development quality and development potential of the mammal oocytes.