Method for analyzing trace substances in to-be-tested sample by utilizing electrospray ionization-mass spectrometry of molecularly imprinted membrane

A technology of molecularly imprinted membranes and template molecules, which is applied in the direction of material analysis, measuring devices, and analysis materials through electromagnetic means, to achieve the effect of improving detection sensitivity, increasing sensitivity, and broad application prospects

Inactive Publication Date: 2017-08-04
ICDC CHINA CDC +1
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problem that the open-type ionization technology cannot satisfy the rapid qualitative and quantitative analysis under the premise of ensuring high sensitivity, the present invention provides a new open-type ionization technology with high sensitivity and no need for sample pretreatment, molecularly imprinted membrane electrospray ionization technology ( MIM-ESI), for food safety, blood drug concentration, antibiotic residues and pesticide residues

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for analyzing trace substances in to-be-tested sample by utilizing electrospray ionization-mass spectrometry of molecularly imprinted membrane
  • Method for analyzing trace substances in to-be-tested sample by utilizing electrospray ionization-mass spectrometry of molecularly imprinted membrane
  • Method for analyzing trace substances in to-be-tested sample by utilizing electrospray ionization-mass spectrometry of molecularly imprinted membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Example 1 Molecularly imprinted membrane surface modification and activation (taking PVDF membrane as an example)

[0064] Immerse the PVDF membrane in a 3% NaOH aqueous solution, treat it at 60°C for 12h, wash the membrane surface with pure water, then immerse the treated membrane in an aqueous solution containing 10% acrylic acid and 1% potassium persulfate, and deoxidize it with nitrogen , Constant temperature reaction at 70°C for 5h. Then, the treated PVDF membrane was soaked in pure water for 1h, and in acetonitrile for 30min, 0.15mol·L -1 The AIBN acetonitrile solution was activated for 20 minutes, and finally the acetonitrile was evaporated and immediately immersed in the molecular imprinting reaction solution.

Embodiment 2

[0065] Example 2 Synthesis of Ractopamine Molecularly Imprinted Membrane

[0066] Weigh 0.0052g template molecule ractopamine (Rac), add 10.43μL functional monomer MAA, fully dissolve with 0.06mL methanol, add 0.3mL chloroform, seal and pre-polymerize at room temperature for 1h. Then add 0.1178mL cross-linking agent EGDMA and 0.0060g initiator AIBN, after completely dissolving, add the surface-modified and activated PVDF membrane prepared in Example 1, remove oxygen by ultrasonication for 10min, and react at room temperature for 2h. Finally, the reacted PVDF membrane was transferred to a nitrogen environment at a constant temperature of 65° C. for 24 h. After the reaction, wash repeatedly with 10% acetic acid methanol solution to remove template molecules, then wash with pure water until neutral, wash with acetone and evaporate to dry, and store the molecularly imprinted membrane in a desiccator for future use.

Embodiment 3

[0067] Synthesis of Example 3 Trimethoprim Molecularly Imprinted Membrane

[0068] Weigh 0.0232g template molecule trimethoprim (TMP), add 34.2μL functional monomer MAA, fully dissolve with 1.5mL chloroform, seal and pre-polymerize at room temperature for 1h. Then add 0.4526mL cross-linking agent EGDMA and 0.0050g initiator AIBN, after completely dissolving, add the surface-modified and activated PVDF membrane prepared in Example 1, remove oxygen by ultrasonication for 10min, and react at room temperature for 2h. Finally, the reacted PVDF membrane was transferred to a nitrogen environment at a constant temperature of 55° C. for 24 h. After the reaction, wash repeatedly with 20% acetic acid methanol solution to remove template molecules, then wash with pure water until neutral, wash with acetone and evaporate to dry, and store the molecularly imprinted membrane in a desiccator for future use.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
correlation coefficientaaaaaaaaaa
Login to view more

Abstract

The invention provides a method for analyzing trace substances in a to-be-tested sample by utilizing an electrospray ionization-mass spectrometry of a molecularly imprinted membrane. The method comprises the following steps: 1) modifying and activating the surface of a microfiltration membrane; 2) soaking the modified and activated microfiltration membrane into a reactive solvent containing template molecules, functional monomers, a crosslinking agent and an initiator, deoxygenizing and reactively synthesizing a molecularly imprinted membrane; 3) soaking the molecularly imprinted membrane synthesized in the step 2) into an elution solvent, shaking at room temperature to remove the template molecules; and 4) adding the molecularly imprinted membrane in the step 3) into the to-be-tested sample, shaking for 10-30min at room temperature, taking out, and detecting the molecularly imprinted membrane by adopting a molecularly imprinted membrane electrospray ionization device. According to the method, sample pretreatment is not needed, the real-time rapid analysis of the practical sample under high sensitivity can be realized, and the method has extremely high application values in the fields of food safety, plasma concentration, antibiotic residues and pesticide residues, and has wide application prospects.

Description

technical field [0001] The present invention relates to the development and application of molecularly imprinted membrane electrospray ionization mass spectrometry (Molecularly imprinted membrane electrospray ionization mass spectrometry, MIM-ESI-MS), in particular, to the workflow of molecularly imprinted membrane electrospray ionization mass spectrometry, and the application of this technology in multiple Applications in various fields, such as rapid detection of actual samples to be tested in food safety, blood drug concentration, antibiotic residues and pesticide residues. Background technique [0002] Mass spectrometry (Mass spectrometry, MS) is a powerful analytical tool, which has the advantages of high sensitivity, high resolution, and small amount of sample analysis, and is widely used in different fields. In recent years, open ionization technology (ambientionization, AM) has become a research hotspot and continues to develop. This technology shortens or avoids the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 张玫徐伟
Owner ICDC CHINA CDC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap