The invention belongs to the technical field of biological sample experiments, and particularly relates to a
synchrotron radiation infrared spectrum miniature vacuum device for biological sample experiments, which comprises a vacuumizing mechanism and a sealing cover, the vacuumizing mechanism comprises a tray, a sealing box, a support pad, a gas
pipe, a miniature
nitrogen cylinder, a gas flow controller, a miniature
vacuum pump and a control valve, a sealing box is arranged at the top of the tray, a supporting pad is arranged at the bottom of an inner cavity of the sealing box, the left side and the right side of the sealing box are communicated with air pipes, and the outer end of the air
pipe on the right side is connected with a miniature
nitrogen cylinder. Or the sealing box is slowly filled with the
nitrogen in the micro
nitrogen cylinder, so that the sealing box is filled with the nitrogen, the
synchrotron radiation infrared spectrum test can be conveniently carried out, result bias is avoided, a treatment mode can be selected according to requirements, and the application range is expanded.