54 results about "Fluorescent spectra" patented technology

An optical sensor patch for measuring water activity is disclosed. The optical sensor patch includes a sheet configured to be in equilibrium with water activity in an environment of the optical sensor patch. A pair of distinct fluorescent dyes are dispersed within the sheet. The fluorescence spectra of the optical sensor patch is dependent on the water activity in the environment of the optical sensor patch. An optical implant and systems and methods for using the optical sensor patch are also disclosed.

The invention discloses an antibiotic pollution self-adaptive detection method based on fluorescence spectrum overlapping degree evaluation. The method comprises the following steps: firstly, carrying out baseline removal, Raman scattering deduction and inner filter effect correction on original excitation-emission matrix (EEM) data; then, determining a scattering area, evaluating the fluorescence intensity gradient, the distribution characteristic and the spectrum shape matching degree in the scattering area, quantifying to obtain an overlapping degree index, and adaptively selecting and implementing a scattering processing strategy based on the overlapping degree index: adopting an interpolation method for a low overlapping condition; for a high overlapping condition, performing fine processing by using an iterative fitting-subtraction method or a deep learning model; and finally, smoothing and standardizing the processed EEM data, and outputting a high-quality fluorescent fingerprint spectrum for antibiotic component identification and qualitative and quantitative analysis. According to the method, effective antibiotic fluorescence information can be reserved to the maximum extent, scattering interference is effectively removed, and the accuracy and reliability of antibiotic pollution detection are remarkably improved.

The application relates to a quality inspection technology of inorganic salt, and discloses a detection reagent and a rapid detection method for high-purity lithium carbonate. A water-soluble fluorescent probe detection reagent is obtained by using 2-(4-chlorophenyl) benzoxazole, 2-(2-hydroxyphenyl) benzoxazole and other benzoxazole compounds as a substrate, and by modifying the substrate with glycerol, tris(hydroxyl) aminomethane and other compounds; high-purity lithium carbonate is detected by using the detection reagent; the high-purity lithium carbonate is solid-phase prepared into lithium carbonate suspension liquid; the upper liquid phase of the lithium carbonate suspension liquid is taken; the fluorescent probe detection reagent is added into the upper liquid phase; and the high-purity lithium carbonate is rapidly detected according to the fluorescent spectrum and a working curve. The application can realize the rapid detection of the high-purity lithium carbonate in a water-soluble environment, and the detection response time can be as short as 3-10 seconds.

A kind of lithium battery electrolyte concentration and moisture fluorescent probe and fluorescent spectrum detection method, metal zirconium salt and organic ligand are dissolved in mixed solvent of organic solvent and water respectively, after heating and carrying out solvothermal reaction, cooling to room temperature, zirconium-based MOF is obtained by separation and purification.The zirconium-based metal organic framework material (MOF) with good specificity fluorescent recognition characteristic for lithium hexafluorophosphate is prepared in the application, which is used for fluorescent probe to detect the concentration and moisture content of lithium hexafluorophosphate in lithium ion battery electrolyte;The application significantly reduces the addition amount of detection agent in electrolyte, has obvious effect, low cost, and has no side effect on the electrochemical performance of electrolyte.

The present invention relates to the fields of chemistry, agriculture, and environmental protection, and discloses a fluorescent probe and its use in fluorescent detection of glyphosate, specifically a fluorescent probe technology for rapid detection of glyphosate in water and agricultural products. The colorimetric fluorescent probe, which combines a 2-chloroaniline sensing unit with the fluorophore dansyl chloride, induces fluorescence enhancement through a substitution reaction with glyphosate. Specifically, the probe can detect glyphosate in the range of 0 to 70 μmol·L. ‑1 Fluorescence titration experiments were conducted with glyphosate at varying concentrations within a specific range. With the gradual addition of glyphosate, the probe's fluorescence spectrum rapidly increased, establishing a satisfactory relationship between fluorescence intensity and glyphosate concentration. Common anions and metal ions found in water did not interfere with the reaction, demonstrating the detection method's excellent anti-interference properties. Furthermore, this probe was used to detect glyphosate in tap water, Xiangxi River Reservoir, soil, soybeans, and corn, with recoveries ranging from 94.7% to 109.9%. This open-type fluorescent probe can specifically detect glyphosate with the naked eye under ultraviolet light.

PPAB derived near-infrared super-small fluorescent silica nanoparticles and synthesis method and application thereof, the fluorescent silica nanoparticles prepared by the present application exhibit strong near-infrared fluorescence and good water solubility, have a strong fluorescence emission peak near 650 nm-700 nm wavelength, exhibit good fluorescence spectrum performance, and therefore the silicon quantum dots have relatively good cell tissue penetration. Due to the unique optical properties and relatively good cell tissue penetration, the in vivo fluorescence imaging can be effectively carried out. The fluorescent silica nanoparticles prepared by the present application have excellent singlet oxygen generation performance and peroxidase performance in addition to excellent near-infrared optical performance and good biocompatibility, can be used as a photodynamic / chemodynamic synergistic treatment reagent, and are used for antibacterial and antitumor applications, and have no toxic side effects on the organism itself.

The invention relates to blue-light zeolite fluorescent powder, a preparation method thereof and a blue-light conversion film. The method comprises the following steps: adding faujasite into a europium chloride solution, and refluxing and stirring at 50-90 DEG C for 10-20 hours to obtain an Eu-doped zeolite material; and then putting the Eu-doped zeolite material into a tubular furnace, calcining for 3-5 hours at 500-900 DEG C in a reducing atmosphere, and cooling to obtain the blue-light zeolite fluorescent powder. The fluorescence spectrum of the fluorescent powder can be adjusted by regulating the silica-alumina ratio of faujasite, the fluorescent powder is used for preparing the light conversion film, and the application of the zeolite fluorescent powder in the aspect of agricultural light conversion films is expanded.

The invention relates to a comprehensive monitoring method and device for coal quality, electronic equipment and a storage medium, and the method comprises the steps: obtaining an image of the surface of a coal flow, and determining a roughness index of the coal flow according to the image; determining the subsample quantity of the coal flow according to the roughness index, and evaluating the uniform distribution condition of the coal flow according to the subsample quantity; detecting the calorific value, moisture, sulfur content and ash content of the coal according to the near-infrared and X-ray fusion fluorescence spectrum to generate coal detection data; comparing the coal detection data with a pre-constructed coal flow evaluation model, and evaluating whether the to-be-detected coal is within a preset normal value range; and managing the coal detection data and the analysis result to generate a coal quality detection report of the to-be-detected coal. Therefore, the problems that real-time rapid detection requirements cannot be met, behavior prediction of the coal under different processing and using conditions is difficult to realize, and accuracy and timeliness of coal quality control are difficult to ensure in the prior art are solved.

The application provides a multi-wavelength correction Raman fluorescence spectrum combined detection device and method, a plurality of wavelength light sources are used to simultaneously perform Raman and fluorescence spectrum detection, Raman and fluorescence spectrum signals excited by the plurality of wavelength light sources are analyzed and mutually corrected, more accurate Raman frequency shift and fluorescence spectrum of a detected substance are obtained, and detection precision is improved.

The invention relates to the technical field of marine oil spill monitoring, in particular to a marine oil spill monitoring method and system based on a multiband fluorescence spectrum neural network. The method comprises the following steps: acquiring fluorescence spectrums and fluorescence image data of spilled oil of different oil types and different oil film thicknesses; respectively preprocessing the fluorescence spectrum and the fluorescence image data of the marine oil spill; constructing three-dimensional fluorescence spectrum data and a thickness difference matrix by using the preprocessed fluorescence spectrum; utilizing a deep neural network to carry out waveband feature and thickness feature extraction on the three-dimensional fluorescence spectrum data and the thickness difference matrix; according to the invention, the marine oil spill fluorescence spectrum and fluorescence image data are effectively integrated, and a reliable basis is provided for accurate identification and early warning of oil spill.

The inventors describe the use of a polymeric marker, in certain aspects the polymeric marker is conjugated polymeric marker such as P-C-3. Other aspects are directed to methods of analyzing the conjugated marker fluorescence spectral shape, which is strongly dependent on the local / ionic environment. This fluorescence marker is able to interact with and classify various analytes.

The present invention provides a method and a system for testing an analyte, a medium, and a device. The method includes: irradiating a first area by broad-spectrum visible light, infrared light, or visible-near-infrared light, and imaging the first area, to obtain an image of an imaging area; irradiating the first area by ultraviolet light within a second wavelength range, and imaging the first area, to obtain a second image of the imaging area; obtaining, from the first image, color or grayscale distribution data that indicate the analyte; based on the color or grayscale distribution data, respectively obtaining, from the first image and the second image, reflection spectral data and fluorescence spectral data at desired positions that indicate the analyte; and obtaining information about the analyte in the imaging area based on the reflection spectral data and fluorescence spectral data.

To provide a method, a system, a medium, and a device for collecting a fluorescence spectrum of an analyte.SOLUTION: Irradiating the first region with infrared light and imaging the first region to obtain a first image of the imaging region, dividing the first region into a detection point candidate region and a reference point candidate region based on a grayscale distribution of the first image reflecting a non-uniform distribution of the analyte in the first region, irradiating the first region with ultraviolet light and imaging the first region based on an excited fluorescence emission signal to obtain a second image of the imaging region, selecting a detection point from the detection point candidate region of the second image and collecting fluorescence spectrum data of the detection point, and selecting a reference point from the reference point candidate region of the second image and collecting fluorescence spectrum data of the reference point.EFFECT: By collecting the fluorescence spectrum data of the blood vessel region and the non-blood vessel region at the same time, interference in subsequent data processing can be eliminated.SELECTED DRAWING: Figure 1

Aspects of the present disclosure include methods for assessing co-linearity between autofluorescence spectra of particles in a sample. A method according to certain embodiments comprises: irradiating a sample comprising particles in a flowing stream with a light source in a flow cytometer; detecting light from the irradiated particles using a light detection system having a photodetector; measuring an autofluorescence spectrum generated by the particles in the sample; and evaluating colinearity between autofluorescence spectra produced by two or more different particles in the sample. Systems and non-transitory computer-readable storage media configured to perform the subject methods are also provided.

One aspect of the present disclosure provides a phosphor powder which contains a phosphor represented by the general formula (Sr1-xEux)3Li0.84yAl6.84+zSi14.16-zOzN28-z [in the general formula, x is at most 0.08, y is 0.02-2.00, and z is 0.50-1.80], and has a light emission peak in a wavelength range of 650-720 nm in fluorescent spectra obtained when radiating light having a wavelength of 450 nm.

The invention discloses a soil petroleum hydrocarbon pollution natural attenuation evaluation method based on fluorescent components, which comprises the following steps: acquiring a three-dimensional fluorescence spectrum of soluble organic matters in petroleum hydrocarbon polluted soil to obtain fluorescence intensity of various fluorescent components; according to the difference between the fluorescence intensities of the multiple fluorescence components, whether the petroleum hydrocarbon in the polluted soil can attenuate naturally or not and the intensity of natural attenuation are evaluated; according to the method, the fluorescence spectrum technology with lower cost is adopted, and the fluorescence components of the soluble organic matters in the polluted soil are analyzed, so that the natural attenuation potential of the petroleum hydrocarbon pollution is judged, and the feasibility of the fluorescence method applied to the identification of the petroleum hydrocarbon pollution of the soil is verified; and a support can be provided for soil environment treatment of a petroleum hydrocarbon polluted site.

The invention relates to the technical field of spectrum detection, in particular to a portable biological powder spectrum detection device which mainly comprises a sample table; a spectrum collection module; the device comprises a fluorescence excitation module, a laser excitation module, a first light path, a second light path and a processing module. The first light path and the second light path partially coincide, the ultraviolet fluorescent light source projects ultraviolet light beams to the to-be-detected sample along the first light path to excite the to-be-detected sample to generate fluorescent light, the fluorescent light generated by the to-be-detected sample retrograde along the first light path and is collected by the spectrum collection module to obtain a fluorescence spectrum, and after the fluorescence spectrum is obtained, the fluorescence excitation module is closed to excite the to-be-detected sample. The laser excitation module projects a laser beam to a to-be-detected sample along a second light path so as to ablate the to-be-detected sample and enable the to-be-detected sample to generate plasmas, optical radiation of the plasmas retrograde along the first light path and is collected by the spectrum collection module to obtain an LIBS spectrum, the processing module cascades the fluorescence spectrum and the LIBS spectrum to generate a feature vector, the feature vector is analyzed, and the detection result is obtained. The type information of the to-be-detected sample is obtained.

The invention relates to the technical field of intelligent medical instruments and wound nursing, in particular to an integrated wound monitoring and repairing method for a fluorescent nano electroactive composite material, which comprises the following steps: synchronously acquiring dual-mode signals of a fluorescence spectrum and an electrochemical impedance spectrum at a wound by using an intelligent dressing integrated with the composite material; extracting a plurality of wound physiological parameters from the dual-mode signal in real time through a signal decoupling model, forming time sequence data, and uploading the time sequence data to a cloud end; the cloud AI model performs association analysis and trend prediction on the time series data to generate a comprehensive diagnosis report; then, the system executes a hierarchical control strategy according to the report: automatically regulating and controlling low-risk parameters, generating a control request for a high-risk condition needing to be treated, manually confirming the control request through a medical care terminal, and then triggering accurate drug release and / or electrical stimulation treatment; in the whole treatment process, manual adjustment or emergency stop is allowed through the medical care terminal. According to the invention, intelligent management from perception, analysis to safety intervention of the wound is realized.

One aspect of the present disclosure provides a phosphor powder which contains a phosphor represented by the general formula (Sr1-xEux)3Li0.84yAl6.84+zSi14.16-zOzN28-z [in the general formula, x is at most 0.08, y is 0.02-2.00, and z is 0.50-1.80], and has a light emission peak in a wavelength range of 650-720 nm in fluorescent spectra obtained when radiating light having a wavelength of 450 nm.

The invention discloses a light path system of an operating microscope. The light path system comprises a visible light unit, a fluorescence unit, an illumination regulation and control module, a light path regulation and control module, a rear light splitting module and an image imaging device, the illumination regulation and control module is used for irradiating a spectrum towards an object surface; the light path regulation and control module is used for enabling the spectrum reflected by the object plane to enter the rear light splitting module; the rear light splitting module is used for transmitting the spectrum to the image imaging device and / or the eyepiece device; the image imaging device is used for realizing fluorescence imaging or visible light imaging; the illumination regulation and control module is also used for forming the visible light spectrum and the fluorescence spectrum into a synthetic spectrum; the image imaging device comprises a driving device and a first fluorescent light filter; and the driving device is used for driving the first fluorescent light filter to move. The system can meet the requirements of a fluorescence mode and a visible light mode, the structure is compact, the imaging effect is improved, and the maintenance cost is reduced.

The present invention provides a method and a system for collecting a fluorescence spectrum of an analyte, and a medium, and a device, which relate to the field of optical analysis. The method includes: irradiating and imaging a first area by infrared light to obtain a first image; dividing the first area into a testing point candidate area and a reference point candidate area based on grayscale distribution indicates uneven distribution of the analyte; irradiating and imaging the first area by ultraviolet light based on an excited fluorescent radiation signal, to obtain a second image; and based on a grayscale value selecting a testing point from the testing point candidate area, and collecting fluorescence spectral data of the testing point; and selecting a reference point from the reference point candidate area and collecting fluorescence spectral data of the reference point.

This invention discloses a method and system for unmixing multicolor fluorescence spectra, relating to the field of multicolor fluorescence imaging. The method includes: constructing an objective function based on a linear spectral mixing model, according to the detected crosstalk signal distribution, the spectral matrix determined by optical system parameters and the emission spectra of each fluorescent probe, and the corresponding compensation matrix, combined with sparse regularization and total variation regularization constraints; determining a computational graph using the alternating direction multiplier method based on the objective function; establishing an end-to-end neural network mapping by adopting a non-shared parameter hierarchical network structure in the computational graph; parameterizing the compensation matrix and inverse matrix in the computational graph based on multiple multi-layer three-dimensional convolutional neural network structures to obtain the neural network; constraining the neural network through a loss function; and training and testing the end-to-end neural network using a multicolor fluorescence crosstalk simulation dataset. This invention can improve the unmixing effect of multicolor fluorescence spectra.

The invention discloses a method, a system and a medium for eliminating interference fluorescence signals in human body detection. The method comprises the following steps: standardizing fluorescence intensities under different wavelengths; defining an optimization objective function and an error value calculation formula; searching an optimal coefficient and a minimum distance by using an optimization algorithm to minimize the deviation between the modeled fluorescence spectrum and the observed fluorescence spectrum; the method is specially used for eliminating crosstalk of non-target fluorescent substances in human body fluorophore information collection, and based on the physical principle, through an accurate mathematical model and algorithm derivation, the optimal coefficient obtained through optimization is used for calculating the final AGEs estimated value, and the correlation coefficient between the estimated value and the actual AGEs value is calculated. And overlapping fluorescence signals are effectively distinguished and processed.

This application belongs to the field of emotion monitoring technology, and provides a method and system for emotion monitoring and intervention based on multimodal neurochemical sensing. It acquires electroacoustic and optical fluorescence spectral signals from a user's target tissue region through a wearable device, and identifies neurotransmitter system activity information through signal fusion and decoding. This information is then input into an individualized dynamic emotion monitoring model to obtain the user's current emotional state type and intensity. Combining these two indicators, an emotion instability index is calculated in real time. When the index exceeds an individualized critical threshold, a personalized intervention plan matching the instability characteristics is generated and executed. The critical threshold can also be dynamically adjusted based on the intervention effect and real-time physiological state. This invention achieves non-invasive neurotransmitter monitoring and emotion monitoring, assessment, and intervention, improving monitoring accuracy and the timeliness and personalization of intervention, and is applicable to fields such as medicine and education.

The invention discloses a miRNA multiple detection method based on a diamond NV color center, which comprises the following steps: firstly, preparing a plasma nano-diamond: modifying amino on the surface of the nano-diamond containing the NV color center, then coupling silver nano-seeds through an electrostatic adsorption effect, and growing silver nano-particles with different particle sizes in situ; plasma nano-diamonds with different ultraviolet absorption peaks are obtained, and then the surfaces of the nano-diamonds and the plasma nano-diamonds are modified with single-chain DNA probes. The multiple detection of miRNA comprises the following steps: firstly, carrying out amination modification on the surface of a quartz substrate and modifying different DNA capture probes, then dropwise adding target miRNA, a nano-diamond DNA probe and a plasma nano-diamond DNA probe, and finally collecting a fluorescence spectrum and an optical detection magnetic resonance spectrum of nanoparticles on the surface of a sample through a microwave module, an optical path system and an infrared radiation system. According to the fluorescence spectrum shape and the optical detection magnetic resonance spectrum resonance frequency, various miRNAs are identified, and simultaneous and in-situ detection of various cancer markers can be realized.

The invention relates to the technical field of chemical molecule concentration detection, in particular to an electrode, system and method for in-situ monitoring based on spectrometry, comprising a gas diffusion layer which is provided with a plurality of pores for gas and light to pass through; the fluorescent probe layer is arranged on the upper end face of the gas diffusion layer, and the projection area of the fluorescent probe layer in the end face direction is D1; the catalyst layer is arranged on the upper end face of the fluorescent probe layer, the projection area of the catalyst layer in the end face direction is D2, and D2 covers D1; wherein the fluorescent probe layer comprises a fluorescent molecular probe which has specific recognition and reversible response to CO2. Compared with a microenvironment monitoring technology for measuring local pH only by using an in-situ Raman spectrum, the method disclosed by the invention has the advantages that the fluorescence probe is added between the catalyst layer and the gas diffusion layer, a fluorescence spectrum signal related to the CO2 concentration is additionally obtained, and in-situ measurement of the local pH and the local CO2 concentration is realized.

The application discloses a novel water-soluble chitosan fluorescent probe and application of the probe in ClO ‑ and NO2 ‑ ion detection. The water-soluble chitosan fluorescent probe has the structural formula as shown in the following. The water-soluble chitosan fluorescent probe can realize qualitative and quantitative detection of ClO ‑ and / or NO2 ‑ ions by a fluorescence spectrum method, and has good anti-interference, high selectivity and sensitivity in the presence of other analytes; meanwhile, the probe can be applied to test paper detection, and has great potential in the detection of ClO ‑ and NO2 ‑ ions.

An apparatus configured for integration into a greenhouse is provided. The apparatus includes a motor and a sheet of woven polymeric material that includes fluorophores. The sheet can be automatically deployed or retracted on demand, via operation of the motor, in order to deliver a dynamic spectrum of light to plants within said greenhouse. In another aspect, an optical element including a sheet with at least two distinct film segments is provided. Each distinct film segment has a different fluorescent spectra than other distinct film segments, said differing fluorescent spectra providing differing wavelength ranges along the length of each distinct segment, such that as the sheet is moved through a greenhouse the spectrum of light changes. An agricultural greenhouse including such an apparatus and / or optical element is provided in another aspect.

To provide a method, a system and a medium for collecting fluorescence spectrum data by using ultraviolet rays.SOLUTION: Irradiating a first region with ultraviolet light having a wavelength of 300 nm to 390 nm, obtaining a fluorescence emission signal emitted by the first region under excitation, the fluorescence emission signal including fluorescence spectrum data, and photographing the fluorescence emission signal to obtain an image, and selecting a data-collection point from the image, and substituting a grayscale value of the data-collection point into a spectrum reconstruction algorithm to calculate and obtain fluorescence spectrum data having a spectrum peak between 400 nm and 800 nm. The technical solutions of the present application can enable wave peaks of spectrum data of various analytes to fall within an identifiable range of the imaging spectrum detection device.SELECTED DRAWING: Figure 1

This invention discloses three methods for reacting with Fe 3+ and Cr 3+ This invention relates to thiophene-containing probes with dual responses and their applications, belonging to the field of organic small molecule fluorescent probes. The probes are labeled FS-1, FS-2, and FS-3. Specifically, probe FS-1, under an excitation spectrum of 555 nm in acetonitrile solution, reacts with Fe... 3+ and Cr 3+ The binding caused a dramatic increase in fluorescence intensity at 584 nm; the FS-2 probe pair, in acetonitrile solution, under an excitation spectrum of 530 nm, reacted with Fe... 3+ and Cr 3+ The binding caused a sharp increase in fluorescence intensity at 555 nm; the FS-3 probe, in acetonitrile solution, under an excitation spectrum of 560 nm, reacted with Fe... 3+ and Cr 3+ The binding caused a sharp increase in fluorescence intensity at 584 nm in the fluorescence spectrum. All three probes were effective against Fe. 3+ and Cr 3+ It has high sensitivity, which is beneficial for detecting food containing these heavy metal ions and for playing a role in environmental monitoring.