98 results about "Fluorescent spectra" patented technology

The invention provides a coating defect detection method, electronic equipment and a storage medium. The method comprises the following steps: uniformly mixing fluorescent powder in coating powder, and cladding the mixed powder on the surface of a substrate to form a coating to be detected; performing point-by-point detection on the to-be-detected coating to obtain an actually measured fluorescence spectrum corresponding to each detection point on the to-be-detected coating; according to the actually measured fluorescence spectrum corresponding to each detection point location and a preset standard fluorescence spectrum, judging whether each detection point location is a defect point location or not, and judging the defect type of the defect point location; and if the defect type is an apparent quality defect, determining a coating quality missing value corresponding to the defect point location, and repairing the defect point location based on the coating quality missing value. According to the invention, the detection efficiency and the detection precision of the coating surface defects can be improved.

The invention belongs to the technical field of regional water environment supervision, and discloses an industrial water pollution traceability method based on a convolutional neural network and water quality fluorescence fingerprints, and the method comprises the steps: obtaining wastewater water quality fluorescence fingerprint information based on a three-dimensional fluorescence spectrum technology, and constructing a CNN pollution traceability classification model by using three-dimensional fluorescence spectrum data as input; and outputting the category of the sample, and inputting the three-dimensional fluorescence spectrum data of the unknown sample into the trained CNN pollution traceability classification model to obtain a suspected pollution source. The industrial water pollution tracing method based on the convolutional neural network and the water quality fluorescent fingerprints is developed for industrial wastewater-urban sewage compound sewage treatment plants and industrial park sewage treatment plants, has the characteristics of simplicity and convenience in operation, low cost, high efficiency and good accuracy, has a relatively high practical application value, and has a wide application prospect. The standard-exceeding emission pollution source can be quickly identified, and technical support is provided for regional water environment supervision.

The invention discloses an ink-uniformizing jet printing process for a battery UV insulating coating, which comprises the following steps: adding a first fluorescent marker and a second fluorescent marker in a UV coating material, the two fluorescent markers being capable of performing fluorescence resonance energy transfer to obtain a marked UV coating material; carrying out multi-position spectral characteristic acquisition on the micro-droplets formed by the UV coating material; dynamically tracking the spreading process of the micro-droplets; exciting the coating in the curing process by adopting multi-wavelength exciting light to obtain cross-linking degree distribution data; and carrying out thermoacoustic scanning on the coating. According to the technical scheme, real-time monitoring of cross-linking degree distribution in the curing process of the UV insulating coating can be achieved, the problem of non-uniform cross-linking of the coating can be found in time by monitoring the whole process of micro-droplet formation, spreading and curing in combination with fluorescence spectrum analysis and thermoacoustic detection technologies, and the quality of the coating is improved. And an effective quality control means is provided for ensuring the long-term reliability of the battery insulating coating.

The invention belongs to the technical field of specific fluorescent probe detection, and particularly relates to a near-infrared fluorescent probe for detecting hydrazine hydrate as well as a preparation method and application of the near-infrared fluorescent probe. The structural formula of the near-infrared fluorescent probe is # imgabs0. The invention provides a preparation method for synthesizing the probe by taking xanthene dye and acetyl chloride as raw materials. By researching the fluorescence spectrum of the fluorescent probe, the selectivity of the probe on N2H4 is evaluated, the fluorescence intensity of the probe is determined to be obviously enhanced only in the presence of N2H4, and the fluorescence intensity is positively correlated with the concentration of N2H4; meanwhile, the probe has the characteristics of quick response and near-infrared emission; the fluorescent probe disclosed by the invention has relatively small cytotoxicity and can be applied to detection of HepG2 cell exogenous hydrazine hydrate.

An optical sensor patch for measuring water activity is disclosed. The optical sensor patch includes a sheet configured to be in equilibrium with water activity in an environment of the optical sensor patch. A pair of distinct fluorescent dyes are dispersed within the sheet. The fluorescence spectra of the optical sensor patch is dependent on the water activity in the environment of the optical sensor patch. An optical implant and systems and methods for using the optical sensor patch are also disclosed.

Systems and methods are provided for multi-spectral or hyper-spectral fluorescence imaging. In one example, a spectral encoding device may be positioned in a detection light path between a detection objective and an imaging sensor of a microscope. In one example, the spectral encoding device includes a first dichroic mirror having a sine transmittance profile and a second dichroic mirror having a cosine transmittance profile. In addition to collecting transmitted light, reflected light from each dichroic mirror is collected and used for total intensity normalization and image analysis.

As a precious historical cultural heritage, the deterioration mode evaluation of the cultural relic stone sculpture is crucial to protection work. A traditional evaluation method depends on manual observation and detection and is low in efficiency and high in subjectivity. The embodiment of the invention relates to the technical field of cultural relic stone carving degradation mode evaluation, and discloses an innovative method. Firstly, collected samples are subjected to microscopic observation and mineral detection, and degradation characteristics are determined; a portable hyperspectral imaging system is used for collecting and calibrating an image of a to-be-evaluated area, and spectral features are extracted, built into a database and processed; a spectral index screening degradation mode is determined through spectral index analysis and image gray scale response correlation and optimization wavelength combination; a WOA-XGBoost algorithm is adopted to train a model, and a stone carving degradation mode is identified to draw a distribution cloud picture; and calculating a weathering coefficient by combining an X-ray fluorescence spectrum result, and evaluating the degradation degree according to the degradation model proportion and the coefficient in the region. According to the method, intelligent identification and rapid evaluation are realized, the labor cost is saved, and the automatic process of cultural relic stone carving exploration is promoted.

This invention proposes a method for detecting chemical oxygen demand (COD) in water based on laser spectroscopy, comprising the following steps: Step 1, shining a laser beam into a water sample to be tested, causing the water sample to generate a light signal upon laser excitation; Step 2, receiving the light signal using a spectrometer, converting it into an electrical signal, and then into a digital signal; Step 3, receiving the digital signal through a data processing system to form a spectral curve; Step 4, calculating the relative integrated fluorescence intensity I based on the information in the spectral curve. T λ1 and λ2 are the starting and ending wavelengths of the fluorescence spectrum, respectively, and Iλ represents the fluorescence intensity at wavelength λ. r This represents the light intensity of the Raman peak after subtracting the fluorescence background; Step 5: Calculate the COD value of the water sample according to the chemical oxygen demand formula for water quality. The chemical oxygen demand formula for water quality is COD = KI T +C. The above detection method can greatly reduce the influence of various interference factors on COD detection results, and has the advantages of high sensitivity, fast measurement speed, convenient measurement, and high accuracy.

The invention belongs to the technical field of fluorescent probes, and particularly discloses a high-sensitivity visual Fe < 3 + > / Al < 3 + > fluorescent probe Rh and a preparation method and application thereof.The high-sensitivity visual Fe < 3 + > / Al < 3 + > fluorescent probe Rh is characterized in that cyanuric chloride and sulfanilic acid are used as raw materials, and an intermediate L1 is synthesized through an alkylation reaction; synthesizing an intermediate L2 by using the intermediate L1 and p-hydroxybenzaldehyde as raw materials through alkylation reaction; the intermediate L2 and rhodamine B hydrazide are subjected to a Schiff base reaction, and the triazine-rhodamine B hydrazide derivative fluorescent probe Rh is obtained. According to the fluorescent probe Rh prepared by the invention, the water solubility is increased due to the introduction of a sulfanilic acid group; the fluorescence spectrum test of the probe Rh and 17 common metal ions shows that the probe Rh has a remarkable fluorescence enhancement effect on Fe < 3 + > and Al < 3 + >; an ultraviolet-visible absorption curve test shows that a new peak appears at 560 nm, the absorbance is remarkably increased, and at 350 nm, Fe < 3 + > has an obvious absorption peak but Al < 3 + > does not exist, and Fe < 3 + > and Al < 3 + > can be distinguished.

The invention discloses an antibiotic pollution self-adaptive detection method based on fluorescence spectrum overlapping degree evaluation. The method comprises the following steps: firstly, carrying out baseline removal, Raman scattering deduction and inner filter effect correction on original excitation-emission matrix (EEM) data; then, determining a scattering area, evaluating the fluorescence intensity gradient, the distribution characteristic and the spectrum shape matching degree in the scattering area, quantifying to obtain an overlapping degree index, and adaptively selecting and implementing a scattering processing strategy based on the overlapping degree index: adopting an interpolation method for a low overlapping condition; for a high overlapping condition, performing fine processing by using an iterative fitting-subtraction method or a deep learning model; and finally, smoothing and standardizing the processed EEM data, and outputting a high-quality fluorescent fingerprint spectrum for antibiotic component identification and qualitative and quantitative analysis. According to the method, effective antibiotic fluorescence information can be reserved to the maximum extent, scattering interference is effectively removed, and the accuracy and reliability of antibiotic pollution detection are remarkably improved.

The application relates to a quality inspection technology of inorganic salt, and discloses a detection reagent and a rapid detection method for high-purity lithium carbonate. A water-soluble fluorescent probe detection reagent is obtained by using 2-(4-chlorophenyl) benzoxazole, 2-(2-hydroxyphenyl) benzoxazole and other benzoxazole compounds as a substrate, and by modifying the substrate with glycerol, tris(hydroxyl) aminomethane and other compounds; high-purity lithium carbonate is detected by using the detection reagent; the high-purity lithium carbonate is solid-phase prepared into lithium carbonate suspension liquid; the upper liquid phase of the lithium carbonate suspension liquid is taken; the fluorescent probe detection reagent is added into the upper liquid phase; and the high-purity lithium carbonate is rapidly detected according to the fluorescent spectrum and a working curve. The application can realize the rapid detection of the high-purity lithium carbonate in a water-soluble environment, and the detection response time can be as short as 3-10 seconds.

Aspects and embodiments relate to apparatus and methods to identify whether a drug is present in a provided sample. One aspect provides a drug detection method to identify whether a drug is present in a provided sample, the method comprising: determining a first fluorescence spectral matrix associated with the provided sample by: excitation of the provided sample at a first excitation wavelength; and receiving a fluorescence emission spectrum generated by the provided sample at the first excitation wavelength; repeating the excitation and receiving steps across a range of excitation wavelengths which differ from the first excitation wavelength; determining a wavelength for irradiation of the sample to induce a photochemical change in the provided sample and irradiating the provided sample with radiation having the determined wavelength; determining a second fluorescence spectral matrix associated with the provided sample by: excitation of the provided sample at a first excitation wavelength; and receiving a fluorescence emission spectrum generated by the provided sample at the first excitation wavelength; repeating the excitation and receiving steps across a range of excitation wavelengths which differ from the first excitation wavelength; comparing the determined first and second fluorescence spectral matrix associated with the provided sample with a library of first and second fluorescence spectral matrices obtained from a plurality of drug samples; and if a match within a predetermined threshold is revealed by said comparison, triggering a positive identification of a drug in the provided sample. Aspects recognise that the rapid detection of certain drugs can be enhanced by augmenting a fluorescence spectral fingerprint based detection methodology with photochemical reactivity tracking. The hybrid photochemical fingerprinting methodology in accordance with aspects allows for capture of information on both: new fluorescent species that emerge as a result of a specifically induced photochemical reaction(s) notable detectable drugs, but also the loss of fluorescent / absorptive species as they are photochemically degraded. Both such physical changes are reflective of the specific chemistry of the analyte (drug) which is being tested for.

The present invention provides methods for quantifying and / or screening for the formation of complexes, in which two of the three compounds of the complex are proteins, one of the two tagged with a fluorescent label and the other of the two tagged with a fluorescence quencher. The third compound is a compound capable of linking those two proteins together, thus forming a ternary complex. The methods of the present invention consist of analysing the changes in fluorescence spectrum that occur following the formation of a complex comprising said compounds, due to the interaction between fluorescent label and fluorescence quencher that formation of said complex brings.

A kind of lithium battery electrolyte concentration and moisture fluorescent probe and fluorescent spectrum detection method, metal zirconium salt and organic ligand are dissolved in mixed solvent of organic solvent and water respectively, after heating and carrying out solvothermal reaction, cooling to room temperature, zirconium-based MOF is obtained by separation and purification.The zirconium-based metal organic framework material (MOF) with good specificity fluorescent recognition characteristic for lithium hexafluorophosphate is prepared in the application, which is used for fluorescent probe to detect the concentration and moisture content of lithium hexafluorophosphate in lithium ion battery electrolyte;The application significantly reduces the addition amount of detection agent in electrolyte, has obvious effect, low cost, and has no side effect on the electrochemical performance of electrolyte.

The present invention relates to the fields of chemistry, agriculture, and environmental protection, and discloses a fluorescent probe and its use in fluorescent detection of glyphosate, specifically a fluorescent probe technology for rapid detection of glyphosate in water and agricultural products. The colorimetric fluorescent probe, which combines a 2-chloroaniline sensing unit with the fluorophore dansyl chloride, induces fluorescence enhancement through a substitution reaction with glyphosate. Specifically, the probe can detect glyphosate in the range of 0 to 70 μmol·L. ‑1 Fluorescence titration experiments were conducted with glyphosate at varying concentrations within a specific range. With the gradual addition of glyphosate, the probe's fluorescence spectrum rapidly increased, establishing a satisfactory relationship between fluorescence intensity and glyphosate concentration. Common anions and metal ions found in water did not interfere with the reaction, demonstrating the detection method's excellent anti-interference properties. Furthermore, this probe was used to detect glyphosate in tap water, Xiangxi River Reservoir, soil, soybeans, and corn, with recoveries ranging from 94.7% to 109.9%. This open-type fluorescent probe can specifically detect glyphosate with the naked eye under ultraviolet light.

PPAB derived near-infrared super-small fluorescent silica nanoparticles and synthesis method and application thereof, the fluorescent silica nanoparticles prepared by the present application exhibit strong near-infrared fluorescence and good water solubility, have a strong fluorescence emission peak near 650 nm-700 nm wavelength, exhibit good fluorescence spectrum performance, and therefore the silicon quantum dots have relatively good cell tissue penetration. Due to the unique optical properties and relatively good cell tissue penetration, the in vivo fluorescence imaging can be effectively carried out. The fluorescent silica nanoparticles prepared by the present application have excellent singlet oxygen generation performance and peroxidase performance in addition to excellent near-infrared optical performance and good biocompatibility, can be used as a photodynamic / chemodynamic synergistic treatment reagent, and are used for antibacterial and antitumor applications, and have no toxic side effects on the organism itself.

The invention discloses a seawater oil spill classification detection device, which belongs to the field of multi-spectral measurement technology, and includes an automatic circulation platform, an optical detection system and an automatic control unit; the automatic circulation platform collects seawater containing oil spills, and sends the seawater sample and the constant temperature mixed liquid of seawater and nanomaterials into the cuvette in a time-sharing manner; the optical detection system uses a laser light source that can excite a fluorescent signal to irradiate the seawater sample in the cuvette to excite the fluorescent signal and collect it to generate fluorescent spectrum data; or uses a laser light source that can excite a Raman light signal to irradiate the constant temperature mixed liquid in the cuvette to excite the Raman light signal and collect it to generate Raman spectrum data; the automatic control unit receives the fluorescent spectrum data and the Raman spectrum data, and executes the seawater oil spill classification detection process. The device of the invention can improve the speed and accuracy of oil spill classification detection, and can realize in-situ detection of seawater, solving the problem of difficulty in tracing the source of oil spills.

The invention discloses a fluorescent probe capable of simultaneously detecting cysteine and tryptophan and a synthesis method and application thereof, the structure of the fluorescent probe is shown as a compound 1, and the prepared fluorescent probe shows relatively strong near-infrared fluorescence and good water solubility, has a strong fluorescence emission peak near the wavelength of 700 nm, and shows good fluorescence spectrum performance. The prepared fluorescent probe shows remarkable specificity and sensitivity in detection of cysteine and tryptophan.

In order to support the efficient analysis of electrophoresis results, the present invention comprises: a fluorescence calibration unit (201) that calculates fluorescence spectral data (D020) of a second specimen (D002) on the basis of signal charge data (D012) of the second specimen (D002); a color conversion processing unit (202) that calculates fluorescent color signal data (D033) on the basis of signal charge data (D013) of a third specimen (D003) and the fluorescence spectral data (D020), and calculates fluorescent color signal data (D031) on the basis of signal charge data (D011) of a first specimen (D001) and the fluorescence spectral data (D020); an intensity correction factor determination unit (203) that calculates an intensity correction factor (D040) from the fluorescent color signal data (D033); an intensity adjustment processing unit (204) that calculates color signal data (D050) by using the fluorescent color signal data (D031) and the intensity correction factor (D040); and a display device (301) that outputs the color signal data (D050).

The invention relates to blue-light zeolite fluorescent powder, a preparation method thereof and a blue-light conversion film. The method comprises the following steps: adding faujasite into a europium chloride solution, and refluxing and stirring at 50-90 DEG C for 10-20 hours to obtain an Eu-doped zeolite material; and then putting the Eu-doped zeolite material into a tubular furnace, calcining for 3-5 hours at 500-900 DEG C in a reducing atmosphere, and cooling to obtain the blue-light zeolite fluorescent powder. The fluorescence spectrum of the fluorescent powder can be adjusted by regulating the silica-alumina ratio of faujasite, the fluorescent powder is used for preparing the light conversion film, and the application of the zeolite fluorescent powder in the aspect of agricultural light conversion films is expanded.

The invention relates to a comprehensive monitoring method and device for coal quality, electronic equipment and a storage medium, and the method comprises the steps: obtaining an image of the surface of a coal flow, and determining a roughness index of the coal flow according to the image; determining the subsample quantity of the coal flow according to the roughness index, and evaluating the uniform distribution condition of the coal flow according to the subsample quantity; detecting the calorific value, moisture, sulfur content and ash content of the coal according to the near-infrared and X-ray fusion fluorescence spectrum to generate coal detection data; comparing the coal detection data with a pre-constructed coal flow evaluation model, and evaluating whether the to-be-detected coal is within a preset normal value range; and managing the coal detection data and the analysis result to generate a coal quality detection report of the to-be-detected coal. Therefore, the problems that real-time rapid detection requirements cannot be met, behavior prediction of the coal under different processing and using conditions is difficult to realize, and accuracy and timeliness of coal quality control are difficult to ensure in the prior art are solved.

The application provides a multi-wavelength correction Raman fluorescence spectrum combined detection device and method, a plurality of wavelength light sources are used to simultaneously perform Raman and fluorescence spectrum detection, Raman and fluorescence spectrum signals excited by the plurality of wavelength light sources are analyzed and mutually corrected, more accurate Raman frequency shift and fluorescence spectrum of a detected substance are obtained, and detection precision is improved.

The invention relates to a building block type fluorescence spectrum detection system and a detection method. The system comprises a building block type bottom plate, a fluorescence excitation module, a filtering focusing module and a fluorescence detection module, the fluorescence excitation module is used for emitting laser and irradiating the laser to a sample to be detected through the dichroscope to excite and generate fluorescence; a driving device of the filtering and focusing module drives a filtering and focusing assembly to move according to the displacement instruction; the fluorescent light is filtered and focused by the filtering and focusing assembly, and the focused fluorescent light passes through a slit of the fluorescence detection module and enters the reflection grating to form diffracted light; the diffracted light is reflected by the reflector to generate reflected light; the imaging assembly collects and processes the reflected light to generate a fluorescence image signal, and sends the fluorescence image signal to the analysis processing device; the analyzing and processing device is used for analyzing and processing the fluorescence image signal to obtain a spectral image of the sample to be detected. Compared with existing fluorescence detection equipment, the building block type fluorescence spectrum detection system is flexible in application, easy to use and low in cost.

The invention discloses a method for realizing multi-state information anti-counterfeiting by stimulating color change of a perovskite / polymer film through a solvent and application. Firstly, a CsPbX3-PVDF fluorescent thin film is prepared, when an acetone solvent acts on the CsPbX3-PVDF fluorescent thin film, the CsPbX3-PVDF fluorescent thin film can interact with PVDF and CsPbX3 crystals, perovskite crystal particles become small, under the quantum confinement effect, the fluorescence spectrum position of the CsPbX3-PVDF fluorescent thin film is subjected to blue shift, and the fluorescence spectrum of the CsPbX3-PVDF fluorescent thin film can be obtained by adjusting the acting time of the acetone solvent on the CsPbX3-PVDF fluorescent thin film and the mass fraction of PVDF in the polymer thin film. The size of the perovskite nanocrystal can be regulated and controlled, so that films with different blue shift degrees are obtained, and different fluorescence color changes are realized. Furthermore, by regulating and controlling the type of CsPbX3, various types of light-emitting changes can be obtained, so that multi-state response is realized, and the method is applied to high-order information encryption. The method provided by the invention is simple and good in stability, and can realize more convenient, low-cost and secure information encryption.

The invention relates to the technical field of marine oil spill monitoring, in particular to a marine oil spill monitoring method and system based on a multiband fluorescence spectrum neural network. The method comprises the following steps: acquiring fluorescence spectrums and fluorescence image data of spilled oil of different oil types and different oil film thicknesses; respectively preprocessing the fluorescence spectrum and the fluorescence image data of the marine oil spill; constructing three-dimensional fluorescence spectrum data and a thickness difference matrix by using the preprocessed fluorescence spectrum; utilizing a deep neural network to carry out waveband feature and thickness feature extraction on the three-dimensional fluorescence spectrum data and the thickness difference matrix; according to the invention, the marine oil spill fluorescence spectrum and fluorescence image data are effectively integrated, and a reliable basis is provided for accurate identification and early warning of oil spill.

Two rhodamine reversible fluorescent sensor derivatives, L1 and L2, bearing 2-methoxy-1-naphthaldehyde and 5-bromo-3-methoxy salicylaldehyde units were synthesized using microwave-assisted organic synthesis and used for selective and sensitive reversible sequential fluorescence detection of aluminum ion (Al3+) and azide (N3−) in aqueous acetonitrile solution via the fluorescence spectral changes. Stoichiometry and binding mechanisms for both sensors are well characterized and established by the respective spectroscopic techniques. L1 and L2 sensors are useful for the analysis of Al3+ and N3− in environmental samples and biological studies

The inventors describe the use of a polymeric marker, in certain aspects the polymeric marker is conjugated polymeric marker such as P-C-3. Other aspects are directed to methods of analyzing the conjugated marker fluorescence spectral shape, which is strongly dependent on the local / ionic environment. This fluorescence marker is able to interact with and classify various analytes.

The present invention provides a method and a system for testing an analyte, a medium, and a device. The method includes: irradiating a first area by broad-spectrum visible light, infrared light, or visible-near-infrared light, and imaging the first area, to obtain an image of an imaging area; irradiating the first area by ultraviolet light within a second wavelength range, and imaging the first area, to obtain a second image of the imaging area; obtaining, from the first image, color or grayscale distribution data that indicate the analyte; based on the color or grayscale distribution data, respectively obtaining, from the first image and the second image, reflection spectral data and fluorescence spectral data at desired positions that indicate the analyte; and obtaining information about the analyte in the imaging area based on the reflection spectral data and fluorescence spectral data.

To provide a method, a system, a medium, and a device for collecting a fluorescence spectrum of an analyte.SOLUTION: Irradiating the first region with infrared light and imaging the first region to obtain a first image of the imaging region, dividing the first region into a detection point candidate region and a reference point candidate region based on a grayscale distribution of the first image reflecting a non-uniform distribution of the analyte in the first region, irradiating the first region with ultraviolet light and imaging the first region based on an excited fluorescence emission signal to obtain a second image of the imaging region, selecting a detection point from the detection point candidate region of the second image and collecting fluorescence spectrum data of the detection point, and selecting a reference point from the reference point candidate region of the second image and collecting fluorescence spectrum data of the reference point.EFFECT: By collecting the fluorescence spectrum data of the blood vessel region and the non-blood vessel region at the same time, interference in subsequent data processing can be eliminated.SELECTED DRAWING: Figure 1

Aspects of the present disclosure include methods for assessing co-linearity between autofluorescence spectra of particles in a sample. A method according to certain embodiments comprises: irradiating a sample comprising particles in a flowing stream with a light source in a flow cytometer; detecting light from the irradiated particles using a light detection system having a photodetector; measuring an autofluorescence spectrum generated by the particles in the sample; and evaluating colinearity between autofluorescence spectra produced by two or more different particles in the sample. Systems and non-transitory computer-readable storage media configured to perform the subject methods are also provided.