34 results about "Fluorescent spectra" patented technology

The application relates to a quality inspection technology of inorganic salt, and discloses a detection reagent and a rapid detection method for high-purity lithium carbonate. A water-soluble fluorescent probe detection reagent is obtained by using 2-(4-chlorophenyl) benzoxazole, 2-(2-hydroxyphenyl) benzoxazole and other benzoxazole compounds as a substrate, and by modifying the substrate with glycerol, tris(hydroxyl) aminomethane and other compounds; high-purity lithium carbonate is detected by using the detection reagent; the high-purity lithium carbonate is solid-phase prepared into lithium carbonate suspension liquid; the upper liquid phase of the lithium carbonate suspension liquid is taken; the fluorescent probe detection reagent is added into the upper liquid phase; and the high-purity lithium carbonate is rapidly detected according to the fluorescent spectrum and a working curve. The application can realize the rapid detection of the high-purity lithium carbonate in a water-soluble environment, and the detection response time can be as short as 3-10 seconds.

A kind of lithium battery electrolyte concentration and moisture fluorescent probe and fluorescent spectrum detection method, metal zirconium salt and organic ligand are dissolved in mixed solvent of organic solvent and water respectively, after heating and carrying out solvothermal reaction, cooling to room temperature, zirconium-based MOF is obtained by separation and purification.The zirconium-based metal organic framework material (MOF) with good specificity fluorescent recognition characteristic for lithium hexafluorophosphate is prepared in the application, which is used for fluorescent probe to detect the concentration and moisture content of lithium hexafluorophosphate in lithium ion battery electrolyte;The application significantly reduces the addition amount of detection agent in electrolyte, has obvious effect, low cost, and has no side effect on the electrochemical performance of electrolyte.

The invention relates to blue-light zeolite fluorescent powder, a preparation method thereof and a blue-light conversion film. The method comprises the following steps: adding faujasite into a europium chloride solution, and refluxing and stirring at 50-90 DEG C for 10-20 hours to obtain an Eu-doped zeolite material; and then putting the Eu-doped zeolite material into a tubular furnace, calcining for 3-5 hours at 500-900 DEG C in a reducing atmosphere, and cooling to obtain the blue-light zeolite fluorescent powder. The fluorescence spectrum of the fluorescent powder can be adjusted by regulating the silica-alumina ratio of faujasite, the fluorescent powder is used for preparing the light conversion film, and the application of the zeolite fluorescent powder in the aspect of agricultural light conversion films is expanded.

The present invention provides a method and a system for testing an analyte, a medium, and a device. The method includes: irradiating a first area by broad-spectrum visible light, infrared light, or visible-near-infrared light, and imaging the first area, to obtain an image of an imaging area; irradiating the first area by ultraviolet light within a second wavelength range, and imaging the first area, to obtain a second image of the imaging area; obtaining, from the first image, color or grayscale distribution data that indicate the analyte; based on the color or grayscale distribution data, respectively obtaining, from the first image and the second image, reflection spectral data and fluorescence spectral data at desired positions that indicate the analyte; and obtaining information about the analyte in the imaging area based on the reflection spectral data and fluorescence spectral data.

To provide a method, a system, a medium, and a device for collecting a fluorescence spectrum of an analyte.SOLUTION: Irradiating the first region with infrared light and imaging the first region to obtain a first image of the imaging region, dividing the first region into a detection point candidate region and a reference point candidate region based on a grayscale distribution of the first image reflecting a non-uniform distribution of the analyte in the first region, irradiating the first region with ultraviolet light and imaging the first region based on an excited fluorescence emission signal to obtain a second image of the imaging region, selecting a detection point from the detection point candidate region of the second image and collecting fluorescence spectrum data of the detection point, and selecting a reference point from the reference point candidate region of the second image and collecting fluorescence spectrum data of the reference point.EFFECT: By collecting the fluorescence spectrum data of the blood vessel region and the non-blood vessel region at the same time, interference in subsequent data processing can be eliminated.SELECTED DRAWING: Figure 1

Aspects of the present disclosure include methods for assessing co-linearity between autofluorescence spectra of particles in a sample. A method according to certain embodiments comprises: irradiating a sample comprising particles in a flowing stream with a light source in a flow cytometer; detecting light from the irradiated particles using a light detection system having a photodetector; measuring an autofluorescence spectrum generated by the particles in the sample; and evaluating colinearity between autofluorescence spectra produced by two or more different particles in the sample. Systems and non-transitory computer-readable storage media configured to perform the subject methods are also provided.

The invention discloses a soil petroleum hydrocarbon pollution natural attenuation evaluation method based on fluorescent components, which comprises the following steps: acquiring a three-dimensional fluorescence spectrum of soluble organic matters in petroleum hydrocarbon polluted soil to obtain fluorescence intensity of various fluorescent components; according to the difference between the fluorescence intensities of the multiple fluorescence components, whether the petroleum hydrocarbon in the polluted soil can attenuate naturally or not and the intensity of natural attenuation are evaluated; according to the method, the fluorescence spectrum technology with lower cost is adopted, and the fluorescence components of the soluble organic matters in the polluted soil are analyzed, so that the natural attenuation potential of the petroleum hydrocarbon pollution is judged, and the feasibility of the fluorescence method applied to the identification of the petroleum hydrocarbon pollution of the soil is verified; and a support can be provided for soil environment treatment of a petroleum hydrocarbon polluted site.

The invention relates to the technical field of spectrum detection, in particular to a portable biological powder spectrum detection device which mainly comprises a sample table; a spectrum collection module; the device comprises a fluorescence excitation module, a laser excitation module, a first light path, a second light path and a processing module. The first light path and the second light path partially coincide, the ultraviolet fluorescent light source projects ultraviolet light beams to the to-be-detected sample along the first light path to excite the to-be-detected sample to generate fluorescent light, the fluorescent light generated by the to-be-detected sample retrograde along the first light path and is collected by the spectrum collection module to obtain a fluorescence spectrum, and after the fluorescence spectrum is obtained, the fluorescence excitation module is closed to excite the to-be-detected sample. The laser excitation module projects a laser beam to a to-be-detected sample along a second light path so as to ablate the to-be-detected sample and enable the to-be-detected sample to generate plasmas, optical radiation of the plasmas retrograde along the first light path and is collected by the spectrum collection module to obtain an LIBS spectrum, the processing module cascades the fluorescence spectrum and the LIBS spectrum to generate a feature vector, the feature vector is analyzed, and the detection result is obtained. The type information of the to-be-detected sample is obtained.

The invention relates to the technical field of intelligent medical instruments and wound nursing, in particular to an integrated wound monitoring and repairing method for a fluorescent nano electroactive composite material, which comprises the following steps: synchronously acquiring dual-mode signals of a fluorescence spectrum and an electrochemical impedance spectrum at a wound by using an intelligent dressing integrated with the composite material; extracting a plurality of wound physiological parameters from the dual-mode signal in real time through a signal decoupling model, forming time sequence data, and uploading the time sequence data to a cloud end; the cloud AI model performs association analysis and trend prediction on the time series data to generate a comprehensive diagnosis report; then, the system executes a hierarchical control strategy according to the report: automatically regulating and controlling low-risk parameters, generating a control request for a high-risk condition needing to be treated, manually confirming the control request through a medical care terminal, and then triggering accurate drug release and / or electrical stimulation treatment; in the whole treatment process, manual adjustment or emergency stop is allowed through the medical care terminal. According to the invention, intelligent management from perception, analysis to safety intervention of the wound is realized.

The present invention provides a method and a system for collecting a fluorescence spectrum of an analyte, and a medium, and a device, which relate to the field of optical analysis. The method includes: irradiating and imaging a first area by infrared light to obtain a first image; dividing the first area into a testing point candidate area and a reference point candidate area based on grayscale distribution indicates uneven distribution of the analyte; irradiating and imaging the first area by ultraviolet light based on an excited fluorescent radiation signal, to obtain a second image; and based on a grayscale value selecting a testing point from the testing point candidate area, and collecting fluorescence spectral data of the testing point; and selecting a reference point from the reference point candidate area and collecting fluorescence spectral data of the reference point.

The invention discloses a method, a system and a medium for eliminating interference fluorescence signals in human body detection. The method comprises the following steps: standardizing fluorescence intensities under different wavelengths; defining an optimization objective function and an error value calculation formula; searching an optimal coefficient and a minimum distance by using an optimization algorithm to minimize the deviation between the modeled fluorescence spectrum and the observed fluorescence spectrum; the method is specially used for eliminating crosstalk of non-target fluorescent substances in human body fluorophore information collection, and based on the physical principle, through an accurate mathematical model and algorithm derivation, the optimal coefficient obtained through optimization is used for calculating the final AGEs estimated value, and the correlation coefficient between the estimated value and the actual AGEs value is calculated. And overlapping fluorescence signals are effectively distinguished and processed.

This application belongs to the field of emotion monitoring technology, and provides a method and system for emotion monitoring and intervention based on multimodal neurochemical sensing. It acquires electroacoustic and optical fluorescence spectral signals from a user's target tissue region through a wearable device, and identifies neurotransmitter system activity information through signal fusion and decoding. This information is then input into an individualized dynamic emotion monitoring model to obtain the user's current emotional state type and intensity. Combining these two indicators, an emotion instability index is calculated in real time. When the index exceeds an individualized critical threshold, a personalized intervention plan matching the instability characteristics is generated and executed. The critical threshold can also be dynamically adjusted based on the intervention effect and real-time physiological state. This invention achieves non-invasive neurotransmitter monitoring and emotion monitoring, assessment, and intervention, improving monitoring accuracy and the timeliness and personalization of intervention, and is applicable to fields such as medicine and education.

The invention discloses a miRNA multiple detection method based on a diamond NV color center, which comprises the following steps: firstly, preparing a plasma nano-diamond: modifying amino on the surface of the nano-diamond containing the NV color center, then coupling silver nano-seeds through an electrostatic adsorption effect, and growing silver nano-particles with different particle sizes in situ; plasma nano-diamonds with different ultraviolet absorption peaks are obtained, and then the surfaces of the nano-diamonds and the plasma nano-diamonds are modified with single-chain DNA probes. The multiple detection of miRNA comprises the following steps: firstly, carrying out amination modification on the surface of a quartz substrate and modifying different DNA capture probes, then dropwise adding target miRNA, a nano-diamond DNA probe and a plasma nano-diamond DNA probe, and finally collecting a fluorescence spectrum and an optical detection magnetic resonance spectrum of nanoparticles on the surface of a sample through a microwave module, an optical path system and an infrared radiation system. According to the fluorescence spectrum shape and the optical detection magnetic resonance spectrum resonance frequency, various miRNAs are identified, and simultaneous and in-situ detection of various cancer markers can be realized.

The invention relates to the technical field of chemical molecule concentration detection, in particular to an electrode, system and method for in-situ monitoring based on spectrometry, comprising a gas diffusion layer which is provided with a plurality of pores for gas and light to pass through; the fluorescent probe layer is arranged on the upper end face of the gas diffusion layer, and the projection area of the fluorescent probe layer in the end face direction is D1; the catalyst layer is arranged on the upper end face of the fluorescent probe layer, the projection area of the catalyst layer in the end face direction is D2, and D2 covers D1; wherein the fluorescent probe layer comprises a fluorescent molecular probe which has specific recognition and reversible response to CO2. Compared with a microenvironment monitoring technology for measuring local pH only by using an in-situ Raman spectrum, the method disclosed by the invention has the advantages that the fluorescence probe is added between the catalyst layer and the gas diffusion layer, a fluorescence spectrum signal related to the CO2 concentration is additionally obtained, and in-situ measurement of the local pH and the local CO2 concentration is realized.

An apparatus configured for integration into a greenhouse is provided. The apparatus includes a motor and a sheet of woven polymeric material that includes fluorophores. The sheet can be automatically deployed or retracted on demand, via operation of the motor, in order to deliver a dynamic spectrum of light to plants within said greenhouse. In another aspect, an optical element including a sheet with at least two distinct film segments is provided. Each distinct film segment has a different fluorescent spectra than other distinct film segments, said differing fluorescent spectra providing differing wavelength ranges along the length of each distinct segment, such that as the sheet is moved through a greenhouse the spectrum of light changes. An agricultural greenhouse including such an apparatus and / or optical element is provided in another aspect.

To provide a method, a system and a medium for collecting fluorescence spectrum data by using ultraviolet rays.SOLUTION: Irradiating a first region with ultraviolet light having a wavelength of 300 nm to 390 nm, obtaining a fluorescence emission signal emitted by the first region under excitation, the fluorescence emission signal including fluorescence spectrum data, and photographing the fluorescence emission signal to obtain an image, and selecting a data-collection point from the image, and substituting a grayscale value of the data-collection point into a spectrum reconstruction algorithm to calculate and obtain fluorescence spectrum data having a spectrum peak between 400 nm and 800 nm. The technical solutions of the present application can enable wave peaks of spectrum data of various analytes to fall within an identifiable range of the imaging spectrum detection device.SELECTED DRAWING: Figure 1

This invention discloses three methods for reacting with Fe 3+ and Cr 3+ This invention relates to thiophene-containing probes with dual responses and their applications, belonging to the field of organic small molecule fluorescent probes. The probes are labeled FS-1, FS-2, and FS-3. Specifically, probe FS-1, under an excitation spectrum of 555 nm in acetonitrile solution, reacts with Fe... 3+ and Cr 3+ The binding caused a dramatic increase in fluorescence intensity at 584 nm; the FS-2 probe pair, in acetonitrile solution, under an excitation spectrum of 530 nm, reacted with Fe... 3+ and Cr 3+ The binding caused a sharp increase in fluorescence intensity at 555 nm; the FS-3 probe, in acetonitrile solution, under an excitation spectrum of 560 nm, reacted with Fe... 3+ and Cr 3+ The binding caused a sharp increase in fluorescence intensity at 584 nm in the fluorescence spectrum. All three probes were effective against Fe. 3+ and Cr 3+ It has high sensitivity, which is beneficial for detecting food containing these heavy metal ions and for playing a role in environmental monitoring.

The application discloses a preparation and application of a near-infrared ratio type bisulfite fluorescent probe, and relates to the technical field of bisulfite fluorescent probes. The probe comprises a near-infrared compound, and a structural formula is as follows: the preparation method and the application of the compound are also provided. The bisulfite ratio fluorescent probe can be reacted with bisulfite to generate a change in a fluorescence spectrum, so that quantitative detection of the bisulfite can be realized. The probe is very sensitive to the reaction with the bisulfite, so that the detection of the bisulfite is facilitated. The probe has good stability, and can be used for a long time. The bisulfite fluorescent probe is a high-selectivity and rapid-response bisulfite fluorescent probe, and is simple to synthesize.

A method for detecting an object containing recycled plastic materials by qualitative and semi-quantitative detections is revealed, in which observing a yellowing condition of the object using a first light source, and illuminating the object using a second light source for observing a defect amount on the surface image of the object. When a yellowing index of the object is smaller than a standard yellowing index as well as a second defect condition is greater than a first defect condition, and an activation energy value of the object from a thermogravimetric analyzer is smaller than a standard activation energy value, the object does contain recycled plastic materials. Since the spectrums of recycled PET and native PET are different, the object contains recycled PET and the proportion of the recycled PET are determined according to the spectrums of fluorescent spectrum analysis and the difference ratio between the spectrums respectively.

The application discloses a fluorescent probe capable of simultaneously detecting cysteine and tryptophan, a synthesis method and application thereof, and a structure as shown in compound 1. The prepared fluorescent probe exhibits strong near-infrared fluorescence and good water solubility, has a strong fluorescence emission peak near 700 nm, and exhibits good fluorescence spectral performance. The prepared fluorescent probe exhibits significant specificity and sensitivity in the detection of cysteine and tryptophan.

The present application relates to the technical field of marine oil spill monitoring, in particular to a marine oil spill monitoring method and system based on multi-band fluorescence spectrum neural network. The method comprises the following steps: acquiring fluorescence spectrum and fluorescence image data of different oil types and different oil film thicknesses of oil spills; preprocessing the fluorescence spectrum and fluorescence image data of marine oil spills respectively; constructing three-dimensional fluorescence spectrum data and thickness difference matrix by using the preprocessed fluorescence spectrum; extracting band features and thickness features of the three-dimensional fluorescence spectrum data and thickness difference matrix by using a deep neural network; the present application effectively integrates marine oil spill fluorescence spectrum and fluorescence image data, and provides a reliable basis for accurate identification and early warning of oil spills.

This disclosure relates to an encoded information system and method. The system includes: an encoded film layer containing multiple luminescent sites formed of a fluorescent material; some or all of the luminescent sites serve as carriers of target information; and a key sequence including at least one mask, each mask having at least one transparent region. Each transparent region is used to measure the fluorescence spectrum of the corresponding luminescent site within the transparent region when the mask covers the encoded film layer. One or more of the following are considered as the key: the shape of the transparent region in each mask, the arrangement of the transparent regions in each mask, the correspondence between various effective wavelengths in the measured fluorescence spectrum under each mask and each bit in the target information, and the order in which the masks are used. The encoded information system in this disclosure satisfies high capacity, high security, and high robustness, while further improving system security.

The invention belongs to the technical field of textile detection, and particularly relates to a textile anti-wrinkle effect detection method based on quantum dot fluorescence. The method aims to solve the problems of high subjectivity, sample destructiveness, low sensitivity and low automation degree in the existing textile anti-wrinkle effect detection method. According to the method, a quantum dot fluorescent probe is loaded on the surface of the textile fabric, fluorescence spectrum data of the textile fabric in flat, wrinkled and recovered states are recorded, and the anti-wrinkle performance of the textile fabric is quantitatively evaluated according to the variable quantity of fluorescence signals in different states. By means of the technical scheme, nondestructive, objective and high-sensitivity detection of the anti-wrinkle effect of the textile fabric is achieved, subjective judgment and sample damage are effectively avoided, the accuracy, repeatability and automation degree of detection are remarkably improved, and therefore an efficient and reliable evaluation means is provided for textile research and development and quality control.

The invention discloses a dicyanoisophorone derivative, a preparation method thereof and application of the dicyanoisophorone derivative as a near-infrared fluorescent probe for detecting COX-2. The structure of the derivative is shown as a formula I. The derivative can respond to COX-2 with high selectivity and high sensitivity, an obvious new emission peak appears in a fluorescence spectrum in the presence of COX-2, the derivative can be used for qualitative and quantitative detection of COX-2 in an aqueous solution and cells, interference of external detection conditions can be greatly reduced, and the derivative has high detection speed and high detection precision. Formula I.

Aspects of the present disclosure include methods for assessing collinearity between autofluorescence spectra of particles in a sample. Methods according to certain embodiments include irradiating a sample having particles in a flow stream with a light source in a flow cytometer, detecting light from the irradiated particles with a light detection system having a photodetector, measuring autofluorescence spectra generated by the particles in the sample and assessing collinearity between the autofluorescence spectra generated by two or more different particles in the sample. Systems and non-transitory computer-readable storage media configured to carry out the subject methods are also provided.

The present application relates to a kind of based on self-adapting detection system and method of unmodified optical fiber probe and spectrum-image fusion, belong to optical fiber fluorescence sensing technical field.The system includes excitation light source, unmodified optical fiber probe, dual-mode signal acquisition unit and signal processing unit.The probe is immersed in the liquid to be measured to excite and collect fluorescence signal;The acquisition unit synchronously obtains fluorescence spectrum signal and fluorescence image signal;The processing unit uses the fusion prediction model based on the range-aware adaptive fusion stacking ensemble (RAAFSE) framework to process dual-mode signal, realizes the quantitative prediction of target concentration.The method simplifies sensor preparation by unmodified probe, improves data dimension using the information complementarity of spectrum and image, and realizes high-precision detection in wide concentration range with the aid of the adaptive fusion capability of RAAFSE framework.The system has high integration, good portability, and can be applied to rapid detection of various target substances by replacing fluorescent indicator.

The invention provides a method for rapidly detecting copper ions, a fluorescent sensor and application of the fluorescent sensor, belongs to the technical field of fluorescent nano sensing, and aims at solving the technical problems that an existing copper ion detection system is complex, operation is tedious and in-situ detection cannot be carried out. The rapid detection method for the copper ions comprises the following steps: mixing ascorbic acid, terephthalic acid and a buffer solution to prepare a standard solution; adding copper ions into the detection liquid, preparing a plurality of parts of detection liquid containing the copper ions with different concentrations, measuring the fluorescence spectrum of the detection liquid, and establishing a linear relation between the copper ion concentration and the fluorescence intensity; adding a to-be-detected solution into the standard solution, determining the fluorescence spectrum to obtain the fluorescence intensity, and obtaining the copper ion concentration in the to-be-detected solution through the linear relationship. According to the present invention, the operation is simple, the price is low, and the result is visual, such that the blood copper detection of the large-scale population can be dealt with, the detection cost and the detection difficulty can be reduced, and the prevention, the early discovery and the early treatment can be performed according to the diseases.

The present invention sets a suitable fluorescence separation method for an object to be measured. An information processing apparatus according to an embodiment includes a separation unit (14) that calculates, from a fluorescence signal measured from a biological sample, fluorescence intensities of one or more fluorescences emitted from one or more fluorescent dyes and auto-fluorescence of the biological sample, by using a calculation using a least square method, the method using a fluorescence spectral reference of the one or more fluorescent dyes and a spontaneous fluorescence spectrum of the biological sample labeled with the fluorescent dyes. In the calculation using the least square method, upper and lower limit values of the fluorescence intensities are set for the one or more fluorescences and the auto-fluorescence.

The application discloses a kind of super-sensitive aluminum ion detection fluorescent probe preparation and application, it is related to converter station valve internal cooling water system aluminum ion fluorescent probe technical field.Probe structure formula is: preparation method is: 4-amino benzhydrazide and 2-hydroxy-1-naphthaldehyde are dissolved in anhydrous ethanol, then reflux, product solution is filtered under vacuum to obtain filtrate, and under the condition of reduced pressure, rotary evaporation dry solvent, obtain benzhydrazide compound based on Schiff base functional group.The application of probe is used to detect the concentration of aluminum ion in sample.The aluminum ion fluorescent probe of the application can be reacted with aluminum ion, and the change of fluorescence spectrum is generated, so that the quantitative detection of aluminum ion is realized.The probe of the application is very sensitive to aluminum ion reaction, so as to facilitate the rapid detection of aluminum ion.The stability of fluorescent probe is good, and then it can be stored for a long time.

The invention relates to the field of fluorescence spectrum microscopic imaging, in particular to a fluorescence microscopic imaging method for polarity of intracellular lipid droplets, which comprises the following steps: selecting a lipid polarity sensitive fluorescent probe Nile red to mark intracellular lipid droplets; controlling the imaging system to continuously scan and irradiate the sample under a plurality of different excitation wavelengths, and cooperating with the camera to acquire an excitation spectrum fluorescence image sequence; an excitation spectrum signal corresponding to each pixel is constructed, discrete Fourier transform is carried out on the excitation spectrum signal, a harmonic component is extracted, and therefore high-dimensional spectrum data is mapped to a two-dimensional phasor space; further carrying out visual color coding analysis on the spectral heterogeneity of the lipid droplets by utilizing the characteristic that a phasor angle corresponds to spectrum movement aiming at the characteristic analysis of a Nile red non-fixed reference spectrum; and finally reconstructing to obtain a spatial distribution map reflecting the polar heterogeneity of the lipid droplets. The method effectively solves the problem that the traditional method is difficult to process the continuous change of the spectrum, and the heterogeneity of the polarity of the lipid droplet in the cell is displayed at high resolution.