79 results about "Fluorescent spectra" patented technology

The invention belongs to the technical field of regional water environment supervision, and discloses an industrial water pollution traceability method based on a convolutional neural network and water quality fluorescence fingerprints, and the method comprises the steps: obtaining wastewater water quality fluorescence fingerprint information based on a three-dimensional fluorescence spectrum technology, and constructing a CNN pollution traceability classification model by using three-dimensional fluorescence spectrum data as input; and outputting the category of the sample, and inputting the three-dimensional fluorescence spectrum data of the unknown sample into the trained CNN pollution traceability classification model to obtain a suspected pollution source. The industrial water pollution tracing method based on the convolutional neural network and the water quality fluorescent fingerprints is developed for industrial wastewater-urban sewage compound sewage treatment plants and industrial park sewage treatment plants, has the characteristics of simplicity and convenience in operation, low cost, high efficiency and good accuracy, has a relatively high practical application value, and has a wide application prospect. The standard-exceeding emission pollution source can be quickly identified, and technical support is provided for regional water environment supervision.

The invention discloses an ink-uniformizing jet printing process for a battery UV insulating coating, which comprises the following steps: adding a first fluorescent marker and a second fluorescent marker in a UV coating material, the two fluorescent markers being capable of performing fluorescence resonance energy transfer to obtain a marked UV coating material; carrying out multi-position spectral characteristic acquisition on the micro-droplets formed by the UV coating material; dynamically tracking the spreading process of the micro-droplets; exciting the coating in the curing process by adopting multi-wavelength exciting light to obtain cross-linking degree distribution data; and carrying out thermoacoustic scanning on the coating. According to the technical scheme, real-time monitoring of cross-linking degree distribution in the curing process of the UV insulating coating can be achieved, the problem of non-uniform cross-linking of the coating can be found in time by monitoring the whole process of micro-droplet formation, spreading and curing in combination with fluorescence spectrum analysis and thermoacoustic detection technologies, and the quality of the coating is improved. And an effective quality control means is provided for ensuring the long-term reliability of the battery insulating coating.

The invention belongs to the technical field of specific fluorescent probe detection, and particularly relates to a near-infrared fluorescent probe for detecting hydrazine hydrate as well as a preparation method and application of the near-infrared fluorescent probe. The structural formula of the near-infrared fluorescent probe is # imgabs0. The invention provides a preparation method for synthesizing the probe by taking xanthene dye and acetyl chloride as raw materials. By researching the fluorescence spectrum of the fluorescent probe, the selectivity of the probe on N2H4 is evaluated, the fluorescence intensity of the probe is determined to be obviously enhanced only in the presence of N2H4, and the fluorescence intensity is positively correlated with the concentration of N2H4; meanwhile, the probe has the characteristics of quick response and near-infrared emission; the fluorescent probe disclosed by the invention has relatively small cytotoxicity and can be applied to detection of HepG2 cell exogenous hydrazine hydrate.

An optical sensor patch for measuring water activity is disclosed. The optical sensor patch includes a sheet configured to be in equilibrium with water activity in an environment of the optical sensor patch. A pair of distinct fluorescent dyes are dispersed within the sheet. The fluorescence spectra of the optical sensor patch is dependent on the water activity in the environment of the optical sensor patch. An optical implant and systems and methods for using the optical sensor patch are also disclosed.

The invention discloses an antibiotic pollution self-adaptive detection method based on fluorescence spectrum overlapping degree evaluation. The method comprises the following steps: firstly, carrying out baseline removal, Raman scattering deduction and inner filter effect correction on original excitation-emission matrix (EEM) data; then, determining a scattering area, evaluating the fluorescence intensity gradient, the distribution characteristic and the spectrum shape matching degree in the scattering area, quantifying to obtain an overlapping degree index, and adaptively selecting and implementing a scattering processing strategy based on the overlapping degree index: adopting an interpolation method for a low overlapping condition; for a high overlapping condition, performing fine processing by using an iterative fitting-subtraction method or a deep learning model; and finally, smoothing and standardizing the processed EEM data, and outputting a high-quality fluorescent fingerprint spectrum for antibiotic component identification and qualitative and quantitative analysis. According to the method, effective antibiotic fluorescence information can be reserved to the maximum extent, scattering interference is effectively removed, and the accuracy and reliability of antibiotic pollution detection are remarkably improved.

The application relates to a quality inspection technology of inorganic salt, and discloses a detection reagent and a rapid detection method for high-purity lithium carbonate. A water-soluble fluorescent probe detection reagent is obtained by using 2-(4-chlorophenyl) benzoxazole, 2-(2-hydroxyphenyl) benzoxazole and other benzoxazole compounds as a substrate, and by modifying the substrate with glycerol, tris(hydroxyl) aminomethane and other compounds; high-purity lithium carbonate is detected by using the detection reagent; the high-purity lithium carbonate is solid-phase prepared into lithium carbonate suspension liquid; the upper liquid phase of the lithium carbonate suspension liquid is taken; the fluorescent probe detection reagent is added into the upper liquid phase; and the high-purity lithium carbonate is rapidly detected according to the fluorescent spectrum and a working curve. The application can realize the rapid detection of the high-purity lithium carbonate in a water-soluble environment, and the detection response time can be as short as 3-10 seconds.

Aspects and embodiments relate to apparatus and methods to identify whether a drug is present in a provided sample. One aspect provides a drug detection method to identify whether a drug is present in a provided sample, the method comprising: determining a first fluorescence spectral matrix associated with the provided sample by: excitation of the provided sample at a first excitation wavelength; and receiving a fluorescence emission spectrum generated by the provided sample at the first excitation wavelength; repeating the excitation and receiving steps across a range of excitation wavelengths which differ from the first excitation wavelength; determining a wavelength for irradiation of the sample to induce a photochemical change in the provided sample and irradiating the provided sample with radiation having the determined wavelength; determining a second fluorescence spectral matrix associated with the provided sample by: excitation of the provided sample at a first excitation wavelength; and receiving a fluorescence emission spectrum generated by the provided sample at the first excitation wavelength; repeating the excitation and receiving steps across a range of excitation wavelengths which differ from the first excitation wavelength; comparing the determined first and second fluorescence spectral matrix associated with the provided sample with a library of first and second fluorescence spectral matrices obtained from a plurality of drug samples; and if a match within a predetermined threshold is revealed by said comparison, triggering a positive identification of a drug in the provided sample. Aspects recognise that the rapid detection of certain drugs can be enhanced by augmenting a fluorescence spectral fingerprint based detection methodology with photochemical reactivity tracking. The hybrid photochemical fingerprinting methodology in accordance with aspects allows for capture of information on both: new fluorescent species that emerge as a result of a specifically induced photochemical reaction(s) notable detectable drugs, but also the loss of fluorescent / absorptive species as they are photochemically degraded. Both such physical changes are reflective of the specific chemistry of the analyte (drug) which is being tested for.

The present invention provides methods for quantifying and / or screening for the formation of complexes, in which two of the three compounds of the complex are proteins, one of the two tagged with a fluorescent label and the other of the two tagged with a fluorescence quencher. The third compound is a compound capable of linking those two proteins together, thus forming a ternary complex. The methods of the present invention consist of analysing the changes in fluorescence spectrum that occur following the formation of a complex comprising said compounds, due to the interaction between fluorescent label and fluorescence quencher that formation of said complex brings.

A kind of lithium battery electrolyte concentration and moisture fluorescent probe and fluorescent spectrum detection method, metal zirconium salt and organic ligand are dissolved in mixed solvent of organic solvent and water respectively, after heating and carrying out solvothermal reaction, cooling to room temperature, zirconium-based MOF is obtained by separation and purification.The zirconium-based metal organic framework material (MOF) with good specificity fluorescent recognition characteristic for lithium hexafluorophosphate is prepared in the application, which is used for fluorescent probe to detect the concentration and moisture content of lithium hexafluorophosphate in lithium ion battery electrolyte;The application significantly reduces the addition amount of detection agent in electrolyte, has obvious effect, low cost, and has no side effect on the electrochemical performance of electrolyte.

The present invention relates to the fields of chemistry, agriculture, and environmental protection, and discloses a fluorescent probe and its use in fluorescent detection of glyphosate, specifically a fluorescent probe technology for rapid detection of glyphosate in water and agricultural products. The colorimetric fluorescent probe, which combines a 2-chloroaniline sensing unit with the fluorophore dansyl chloride, induces fluorescence enhancement through a substitution reaction with glyphosate. Specifically, the probe can detect glyphosate in the range of 0 to 70 μmol·L. ‑1 Fluorescence titration experiments were conducted with glyphosate at varying concentrations within a specific range. With the gradual addition of glyphosate, the probe's fluorescence spectrum rapidly increased, establishing a satisfactory relationship between fluorescence intensity and glyphosate concentration. Common anions and metal ions found in water did not interfere with the reaction, demonstrating the detection method's excellent anti-interference properties. Furthermore, this probe was used to detect glyphosate in tap water, Xiangxi River Reservoir, soil, soybeans, and corn, with recoveries ranging from 94.7% to 109.9%. This open-type fluorescent probe can specifically detect glyphosate with the naked eye under ultraviolet light.

PPAB derived near-infrared super-small fluorescent silica nanoparticles and synthesis method and application thereof, the fluorescent silica nanoparticles prepared by the present application exhibit strong near-infrared fluorescence and good water solubility, have a strong fluorescence emission peak near 650 nm-700 nm wavelength, exhibit good fluorescence spectrum performance, and therefore the silicon quantum dots have relatively good cell tissue penetration. Due to the unique optical properties and relatively good cell tissue penetration, the in vivo fluorescence imaging can be effectively carried out. The fluorescent silica nanoparticles prepared by the present application have excellent singlet oxygen generation performance and peroxidase performance in addition to excellent near-infrared optical performance and good biocompatibility, can be used as a photodynamic / chemodynamic synergistic treatment reagent, and are used for antibacterial and antitumor applications, and have no toxic side effects on the organism itself.

The invention relates to blue-light zeolite fluorescent powder, a preparation method thereof and a blue-light conversion film. The method comprises the following steps: adding faujasite into a europium chloride solution, and refluxing and stirring at 50-90 DEG C for 10-20 hours to obtain an Eu-doped zeolite material; and then putting the Eu-doped zeolite material into a tubular furnace, calcining for 3-5 hours at 500-900 DEG C in a reducing atmosphere, and cooling to obtain the blue-light zeolite fluorescent powder. The fluorescence spectrum of the fluorescent powder can be adjusted by regulating the silica-alumina ratio of faujasite, the fluorescent powder is used for preparing the light conversion film, and the application of the zeolite fluorescent powder in the aspect of agricultural light conversion films is expanded.

The invention relates to a comprehensive monitoring method and device for coal quality, electronic equipment and a storage medium, and the method comprises the steps: obtaining an image of the surface of a coal flow, and determining a roughness index of the coal flow according to the image; determining the subsample quantity of the coal flow according to the roughness index, and evaluating the uniform distribution condition of the coal flow according to the subsample quantity; detecting the calorific value, moisture, sulfur content and ash content of the coal according to the near-infrared and X-ray fusion fluorescence spectrum to generate coal detection data; comparing the coal detection data with a pre-constructed coal flow evaluation model, and evaluating whether the to-be-detected coal is within a preset normal value range; and managing the coal detection data and the analysis result to generate a coal quality detection report of the to-be-detected coal. Therefore, the problems that real-time rapid detection requirements cannot be met, behavior prediction of the coal under different processing and using conditions is difficult to realize, and accuracy and timeliness of coal quality control are difficult to ensure in the prior art are solved.

The application provides a multi-wavelength correction Raman fluorescence spectrum combined detection device and method, a plurality of wavelength light sources are used to simultaneously perform Raman and fluorescence spectrum detection, Raman and fluorescence spectrum signals excited by the plurality of wavelength light sources are analyzed and mutually corrected, more accurate Raman frequency shift and fluorescence spectrum of a detected substance are obtained, and detection precision is improved.

The invention discloses a method for realizing multi-state information anti-counterfeiting by stimulating color change of a perovskite / polymer film through a solvent and application. Firstly, a CsPbX3-PVDF fluorescent thin film is prepared, when an acetone solvent acts on the CsPbX3-PVDF fluorescent thin film, the CsPbX3-PVDF fluorescent thin film can interact with PVDF and CsPbX3 crystals, perovskite crystal particles become small, under the quantum confinement effect, the fluorescence spectrum position of the CsPbX3-PVDF fluorescent thin film is subjected to blue shift, and the fluorescence spectrum of the CsPbX3-PVDF fluorescent thin film can be obtained by adjusting the acting time of the acetone solvent on the CsPbX3-PVDF fluorescent thin film and the mass fraction of PVDF in the polymer thin film. The size of the perovskite nanocrystal can be regulated and controlled, so that films with different blue shift degrees are obtained, and different fluorescence color changes are realized. Furthermore, by regulating and controlling the type of CsPbX3, various types of light-emitting changes can be obtained, so that multi-state response is realized, and the method is applied to high-order information encryption. The method provided by the invention is simple and good in stability, and can realize more convenient, low-cost and secure information encryption.

The invention relates to the technical field of marine oil spill monitoring, in particular to a marine oil spill monitoring method and system based on a multiband fluorescence spectrum neural network. The method comprises the following steps: acquiring fluorescence spectrums and fluorescence image data of spilled oil of different oil types and different oil film thicknesses; respectively preprocessing the fluorescence spectrum and the fluorescence image data of the marine oil spill; constructing three-dimensional fluorescence spectrum data and a thickness difference matrix by using the preprocessed fluorescence spectrum; utilizing a deep neural network to carry out waveband feature and thickness feature extraction on the three-dimensional fluorescence spectrum data and the thickness difference matrix; according to the invention, the marine oil spill fluorescence spectrum and fluorescence image data are effectively integrated, and a reliable basis is provided for accurate identification and early warning of oil spill.

Two rhodamine reversible fluorescent sensor derivatives, L1 and L2, bearing 2-methoxy-1-naphthaldehyde and 5-bromo-3-methoxy salicylaldehyde units were synthesized using microwave-assisted organic synthesis and used for selective and sensitive reversible sequential fluorescence detection of aluminum ion (Al3+) and azide (N3−) in aqueous acetonitrile solution via the fluorescence spectral changes. Stoichiometry and binding mechanisms for both sensors are well characterized and established by the respective spectroscopic techniques. L1 and L2 sensors are useful for the analysis of Al3+ and N3− in environmental samples and biological studies

The inventors describe the use of a polymeric marker, in certain aspects the polymeric marker is conjugated polymeric marker such as P-C-3. Other aspects are directed to methods of analyzing the conjugated marker fluorescence spectral shape, which is strongly dependent on the local / ionic environment. This fluorescence marker is able to interact with and classify various analytes.

The present invention provides a method and a system for testing an analyte, a medium, and a device. The method includes: irradiating a first area by broad-spectrum visible light, infrared light, or visible-near-infrared light, and imaging the first area, to obtain an image of an imaging area; irradiating the first area by ultraviolet light within a second wavelength range, and imaging the first area, to obtain a second image of the imaging area; obtaining, from the first image, color or grayscale distribution data that indicate the analyte; based on the color or grayscale distribution data, respectively obtaining, from the first image and the second image, reflection spectral data and fluorescence spectral data at desired positions that indicate the analyte; and obtaining information about the analyte in the imaging area based on the reflection spectral data and fluorescence spectral data.

To provide a method, a system, a medium, and a device for collecting a fluorescence spectrum of an analyte.SOLUTION: Irradiating the first region with infrared light and imaging the first region to obtain a first image of the imaging region, dividing the first region into a detection point candidate region and a reference point candidate region based on a grayscale distribution of the first image reflecting a non-uniform distribution of the analyte in the first region, irradiating the first region with ultraviolet light and imaging the first region based on an excited fluorescence emission signal to obtain a second image of the imaging region, selecting a detection point from the detection point candidate region of the second image and collecting fluorescence spectrum data of the detection point, and selecting a reference point from the reference point candidate region of the second image and collecting fluorescence spectrum data of the reference point.EFFECT: By collecting the fluorescence spectrum data of the blood vessel region and the non-blood vessel region at the same time, interference in subsequent data processing can be eliminated.SELECTED DRAWING: Figure 1

Aspects of the present disclosure include methods for assessing co-linearity between autofluorescence spectra of particles in a sample. A method according to certain embodiments comprises: irradiating a sample comprising particles in a flowing stream with a light source in a flow cytometer; detecting light from the irradiated particles using a light detection system having a photodetector; measuring an autofluorescence spectrum generated by the particles in the sample; and evaluating colinearity between autofluorescence spectra produced by two or more different particles in the sample. Systems and non-transitory computer-readable storage media configured to perform the subject methods are also provided.

One aspect of the present disclosure provides a phosphor powder which contains a phosphor represented by the general formula (Sr1-xEux)3Li0.84yAl6.84+zSi14.16-zOzN28-z [in the general formula, x is at most 0.08, y is 0.02-2.00, and z is 0.50-1.80], and has a light emission peak in a wavelength range of 650-720 nm in fluorescent spectra obtained when radiating light having a wavelength of 450 nm.

A fluorescent spectrum correcting method includes comparing fluorescent spectrum obtained from micro-particles labeled with a plurality of fluorescent pigments with reference spectrum to separating the fluorescent spectrum into fluorescent spectrum for each pigment, and previously measured spectrum data is used as the reference spectrum.

The invention discloses a novel water-soluble chitosan fluorescent probe and application of the novel water-soluble chitosan fluorescent probe in ClO <-> and NO2 <-> ion detection. The structural formula of the water-soluble chitosan fluorescent probe is as follows: # imgabs0 #. The water-soluble chitosan fluorescent probe disclosed by the invention can realize qualitative and quantitative detection of ClO <-> and / or NO2 <-> ions through fluorescence spectrometry, and has good anti-interference performance, high selectivity and sensitivity in the presence of other analytes; meanwhile, the probe can be applied to test paper detection and shows huge potential in the aspect of detecting ClO <-> and NO2 <-> ions.

The invention discloses a soil petroleum hydrocarbon pollution natural attenuation evaluation method based on fluorescent components, which comprises the following steps: acquiring a three-dimensional fluorescence spectrum of soluble organic matters in petroleum hydrocarbon polluted soil to obtain fluorescence intensity of various fluorescent components; according to the difference between the fluorescence intensities of the multiple fluorescence components, whether the petroleum hydrocarbon in the polluted soil can attenuate naturally or not and the intensity of natural attenuation are evaluated; according to the method, the fluorescence spectrum technology with lower cost is adopted, and the fluorescence components of the soluble organic matters in the polluted soil are analyzed, so that the natural attenuation potential of the petroleum hydrocarbon pollution is judged, and the feasibility of the fluorescence method applied to the identification of the petroleum hydrocarbon pollution of the soil is verified; and a support can be provided for soil environment treatment of a petroleum hydrocarbon polluted site.

The invention relates to the technical field of spectrum detection, in particular to a portable biological powder spectrum detection device which mainly comprises a sample table; a spectrum collection module; the device comprises a fluorescence excitation module, a laser excitation module, a first light path, a second light path and a processing module. The first light path and the second light path partially coincide, the ultraviolet fluorescent light source projects ultraviolet light beams to the to-be-detected sample along the first light path to excite the to-be-detected sample to generate fluorescent light, the fluorescent light generated by the to-be-detected sample retrograde along the first light path and is collected by the spectrum collection module to obtain a fluorescence spectrum, and after the fluorescence spectrum is obtained, the fluorescence excitation module is closed to excite the to-be-detected sample. The laser excitation module projects a laser beam to a to-be-detected sample along a second light path so as to ablate the to-be-detected sample and enable the to-be-detected sample to generate plasmas, optical radiation of the plasmas retrograde along the first light path and is collected by the spectrum collection module to obtain an LIBS spectrum, the processing module cascades the fluorescence spectrum and the LIBS spectrum to generate a feature vector, the feature vector is analyzed, and the detection result is obtained. The type information of the to-be-detected sample is obtained.

The invention relates to the technical field of intelligent medical instruments and wound nursing, in particular to an integrated wound monitoring and repairing method for a fluorescent nano electroactive composite material, which comprises the following steps: synchronously acquiring dual-mode signals of a fluorescence spectrum and an electrochemical impedance spectrum at a wound by using an intelligent dressing integrated with the composite material; extracting a plurality of wound physiological parameters from the dual-mode signal in real time through a signal decoupling model, forming time sequence data, and uploading the time sequence data to a cloud end; the cloud AI model performs association analysis and trend prediction on the time series data to generate a comprehensive diagnosis report; then, the system executes a hierarchical control strategy according to the report: automatically regulating and controlling low-risk parameters, generating a control request for a high-risk condition needing to be treated, manually confirming the control request through a medical care terminal, and then triggering accurate drug release and / or electrical stimulation treatment; in the whole treatment process, manual adjustment or emergency stop is allowed through the medical care terminal. According to the invention, intelligent management from perception, analysis to safety intervention of the wound is realized.

One aspect of the present disclosure provides a phosphor powder which contains a phosphor represented by the general formula (Sr1-xEux)3Li0.84yAl6.84+zSi14.16-zOzN28-z [in the general formula, x is at most 0.08, y is 0.02-2.00, and z is 0.50-1.80], and has a light emission peak in a wavelength range of 650-720 nm in fluorescent spectra obtained when radiating light having a wavelength of 450 nm.

The invention discloses a light path system of an operating microscope. The light path system comprises a visible light unit, a fluorescence unit, an illumination regulation and control module, a light path regulation and control module, a rear light splitting module and an image imaging device, the illumination regulation and control module is used for irradiating a spectrum towards an object surface; the light path regulation and control module is used for enabling the spectrum reflected by the object plane to enter the rear light splitting module; the rear light splitting module is used for transmitting the spectrum to the image imaging device and / or the eyepiece device; the image imaging device is used for realizing fluorescence imaging or visible light imaging; the illumination regulation and control module is also used for forming the visible light spectrum and the fluorescence spectrum into a synthetic spectrum; the image imaging device comprises a driving device and a first fluorescent light filter; and the driving device is used for driving the first fluorescent light filter to move. The system can meet the requirements of a fluorescence mode and a visible light mode, the structure is compact, the imaging effect is improved, and the maintenance cost is reduced.

The present invention discloses a ratiometric near-infrared fluorescent probe, its synthesis method and applications, belonging to the field of organic small molecule ratiometric fluorescent probes. The molecular formula of the near-infrared fluorescent probe is C 32 H 31 N2O3 + , labeled as FS-B1. The maximum absorption wavelength of the near-infrared fluorescent probe in acetonitrile and PBS (30% ACN) is 640 nm, the fluorescence spectrum wavelength is 765 nm, the fluorescence performance is stable, and the response time is fast. In acetonitrile, as the concentration of organic amine increases, and in PBS (30% ACN), as the concentration of NaHSO3 increases, the emission peak of the near-infrared fluorescent probe at 765 nm gradually decreases, the emission peak at 483 nm gradually increases, the absorption peak at 640 nm gradually decreases, the absorption peak at 400 nm gradually increases, and the color of the solution changes significantly and can be distinguished by the naked eye. The near-infrared fluorescent probe realizes the detection of trace amounts of organic amines and NaHSO3 with high sensitivity, and can efficiently and selectively detect trace amounts of biogenic amines produced during food spoilage, showing good application prospects in the field of food safety.