The invention discloses preparation and an application of a fluorescent compound having a sensing function on methamphetamine and methamphetamine analogues and preparation and an application of a fluorescent sensing thin film, and belongs to the technical field of fluorescent compound preparation. The fluorescent compound contains a cholesterol fragment and a 3,4,9,10-perylenetetracarboxylic acid anhydride unit, the cholesterol fragment has the advantages of rigid skeleton, multiple chiral centers and strong van der Waals effect and the like, 3,4,9,10-perylenetetracarboxylic acid anhydride has high fluorescence quantum yield, good photochemistry stability and strong pi-pi stacking interaction, and the construction units make the prepared fluorescent compound have supramolecular characteristics and extremely rich assembly behaviors simultaneously. The prepared sensing thin film has the advantages of controllable micro-morphology, good stability and high sensitivity, and can be used repeatedly; through device formation and combining development with a dedicated fluorescence detection platform, the sensing thin film is expected to be used for a dedicated detector for super sensitive gas-phase detection of methamphetamine and the methamphetamine analogues, and provides a technical support for drug investigation detective works.