680 results about "Drug detection" patented technology

The invention belongs to the technical field of medicines, and provides a cassia twig granular preparation as well as a preparation method and a product thereof. The cassia twig granule preparation is prepared from the following components in parts by weight: 35 to 50 parts of cassia twig granule extract dry paste and 50 to 105 parts of auxiliary materials, the auxiliary materials comprise lactose, beta-cyclodextrin and derivatives thereof, and citric acid. According to the invention, beta-cyclodextrin and derivatives thereof are used for replacing dextrin as an auxiliary material in the cassia twig granules, so that the interference problem of dextrin in drug detection is avoided, the formability, solubility and hygroscopicity of the cassia twig granule preparation are ensured, the stability of the preparation is improved, and the comprehensive performance is excellent.

The invention discloses a liquid medicine foreign matter detection method and system based on machine vision, and the method comprises the steps: obtaining multiband spectral data of a liquid medicine sample, and carrying out the normalization processing to generate a liquid medicine background standard spectral feature template; comparing the refractive index spectral characteristics of the template and the foreign matter-containing liquid medicine, and recording the surface roughness scattering mode and polarized light reflection angle change data of a foreign matter area; calculating a spectrum peak displacement quantized value according to the polarized light data, and extracting foreign matter edge spectrum gradient information in combination with a threshold value; calculating a transparency spectral attenuation coefficient based on the edge gradient information, and obtaining spectral contrast enhancement factors of different foreign matters in combination with the spectral response characteristic data; and if the enhancement factor does not reach the standard, adjusting the spectrum correction parameter and recalculating, fusing the scattering mode and the multi-angle incident spectrum response result to carry out consistency verification, and obtaining a final identification result containing the foreign matter material type and the danger level. The method can improve the drug detection accuracy and guarantee the drug quality safety.

The utility model relates to the field of medicine detection, and discloses a medicine detection titration device which comprises a base, a fixing rod and a supporting strip, the top surface of the base is connected with the fixing rod, the outer circle wall surface of the fixing rod is provided with the supporting strip, the top surface of the supporting strip is provided with a first fixing hole, the fixing rod is sleeved with the first fixing hole, and the supporting strip is provided with a second fixing hole. Two sides of the supporting strip are connected with fixing rings, two loading strips are arranged on the top surface of the base, a plurality of accommodating grooves are formed in the top surfaces of the loading strips, the accommodating grooves correspond to the fixing rings in position, and two sliding grooves are formed in the top surface of the base. The beaker can be directly taken out from the interior of the containing groove, on the other hand, a titration reagent of the burette can react with different solutions to be detected, one burette can react with the different solutions to be detected, the beaker can be taken out more easily, and more efficiency and convenience are achieved.

The invention provides a construction method and application of a fingerprint spectrum of a dampness-removing stomach-poria cocos decoction, and belongs to the technical field of drug detection.The construction method comprises the steps that the dampness-removing stomach-poria cocos decoction is used for preparing a test solution, and then the fingerprint spectrum of the dampness-removing stomach-poria cocos decoction is obtained through high performance liquid chromatography detection with acetonitrile (A)-0.18-0.22 wt% formic acid aqueous solution as a mobile phase; a standard fingerprint spectrum is established by adopting a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system and is used for quality evaluation or control in the whole process of research / development / production / clinical application of the dampness-removing stomach-poria decoction. According to the construction method of the fingerprint spectrum of the dampness-eliminating stomach-poria cocos decoction, main medicinal material components in the dampness-eliminating stomach-poria cocos decoction can be detected only through high performance liquid chromatography, so that the quality condition of the dampness-eliminating stomach-poria cocos decoction can be reflected more comprehensively.

The invention provides a construction method and application of an angelica sinensis potion fingerprint spectrum, and belongs to the technical field of drug detection.The construction method includes the steps that firstly, an angelica sinensis potion is used for preparing a test solution, then 0.08-0.12 wt% phosphoric acid aqueous solution (A)-acetonitrile (B) serves as a mobile phase, and the angelica sinensis potion fingerprint spectrum is obtained through high performance liquid chromatography detection, a standard fingerprint spectrum is established and is used for quality evaluation or control in the whole process of research / development / production / clinical application of the angelica sinensis potion. According to the construction method of the fingerprint spectrum of the angelica sinensis potion, main medicinal material components in the angelica sinensis potion can be detected only through high performance liquid chromatography, so that the quality condition of the angelica sinensis potion is reflected more comprehensively.

The utility model provides a medicine detecting and rejecting device which comprises a support, a vacuum blowing block, a vacuum suction pipe, a vacuum exhaust pipe and a rejecting and receiving box, the vacuum blowing block and the rejecting and receiving box are respectively installed on the support, and the vacuum blowing block is provided with a plurality of hole channels. Each hole channel is correspondingly provided with a first air inlet hole, a first exhaust hole and a positive pressure hole, meanwhile, each positive pressure hole is connected with a positive pressure air source, each first air inlet hole is connected with a vacuum suction pipe, and each first exhaust hole is connected with a vacuum exhaust pipe; and one end, far away from the vacuum blowing block, of the vacuum exhaust pipe extends into the waste rejecting and receiving box. The utility model provides a vacuum negative pressure rejecting mode, and unqualified medicines are sucked into the waste box by utilizing vacuum, so that not only can the rejecting of the unqualified medicines be effectively ensured, but also the splashing of the unqualified medicines is avoided.

The invention discloses a detection method for determining N-nitroso-Vonoprasan in Vonoprasan fumarate, and belongs to the technical field of medicine detection.The detection method comprises the steps that a solution is prepared, and detection is conducted; the detection comprises the following steps: determining a test solution and a reference solution by adopting a liquid chromatography-mass spectrometry method, recording a chromatogram, and calculating the content of N-nitroso-Vonorazan according to the chromatogram and an external standard method; according to the method, a triple quadrupole rod liquid phase mass spectrometer is adopted; a chromatographic column adopts octadecyl silane bonded silica gel as a filling agent, the specification of the chromatographic column is Poroshell 120EC-C18, 1.9 mu m, 50 mm * 3.0 mm; a mobile phase is composed of a mobile phase A and a mobile phase B, and gradient elution is adopted; the ultra-high pressure chromatographic column is adopted, the operation time is shorter, the signal-to-noise ratio is higher, the detection sensitivity is higher, the detection cost is lower, and the detection requirement can be met on a low-end triple quadrupole mass spectrum.

The invention belongs to the technical field of drug detection, and particularly relates to a PCR primer pair and / or probe for detecting adulterated fritillaria in bulbus fritillariae cirrhosae or related products of bulbus fritillariae cirrhosae and application of the PCR primer pair and / or probe. Based on the PCR primer pair and / or probe provided by the invention, the adulterated fritillaria in the bulbus fritillariae cirrhosae or related products thereof is detected, the sensitivity is high, the repeatability and the precision are good, and qualitative or quantitative detection of the adulterated fritillaria in the bulbus fritillariae cirrhosae or related products thereof can be accurately realized.

The present application discloses a method for determining the content of four active ingredients in Yanli Shuang oral dripping pills in the field of drug detection technology. A method for simultaneously detecting the content of four active ingredients, camphor, menthone, borneol and menthol in Yanli Shuang oral dripping pills, is established by using the GC-MS internal standard method. The method is accurate, simple and has good repeatability, and can be used for the quality control of Yanli Shuang oral dripping pills. The present invention also uses GC-MS to establish a fingerprint of Yanli Shuang oral dripping pills, which has the advantages of saving time and high sensitivity, and provides a scientific basis for clarifying the material basis of drug efficacy and quality control based on volatile components.

The invention relates to a pathological pulmonary fibrosis model and a construction method and application thereof. The pathological pulmonary fibrosis model provided by the invention is good in stability and controllability and high in maturity, not only can better simulate the function and structural characteristics of pulmonary alveoli-capillary barrier, but also has the pathological characteristics and immune microenvironment of pulmonary fibrosis lesions, has the advantage of high simulation degree, and has good application prospects in simulation and research of pulmonary fibrosis diseases. Good application prospects are realized in the aspects of drug detection, screening, evaluation, research and development and the like.

Provided is a mammalian-derived cell model that can be used in anti-aging drugs. The mammalian-derived extraembryonic trophoblast cell model is an early mammalian-derived extraembryonic stage. A change model and an aging evaluation index system for differentiating the cells into the extraembryonic trophoblast cells / organoids can be used for various anti-aging researches and target detection, anti-aging drug detection, screening and identification, research on innovative drugs / natural products, and development of chemicals and / or health foods and additives. Screening and identifying many resistances is helpful. Candidate targets of aging may be further mechanically and functionally validated for future therapeutic use. The screening of anti-aging drugs or natural products will produce huge economic and social benefits.

The invention relates to the technical field of medicine detection, in particular to a detection method of sildenafil citrate tablets, which comprises the following steps: S1, placing contents and target samples with different water contents in a plurality of containers; s2, determining the drug concentration of each region based on the color development characteristics of the diffused image of the target sample in the container at different detection time points, and calculating the concentration gradient component and diffusion concentration gradient of the target sample; s3, measuring the total amount or the concentration of the residual medicine of the target sample in the plurality of containers in a preset time period so as to determine the residual degree of the target sample; and S4, determining the diffusion difference degree based on the diffusion concentration gradient and the residual degree, and evaluating the diffusion adaptability of the target sample based on the diffusion difference degree. The accuracy and depth of drug diffusion performance research are improved, reliable data support is further provided for drug research and development and quality control, and drug quality can be improved.

The invention belongs to the technical field of immunodetection, and discloses a hapten and an artificial antigen for sulfonamide drug detection, a construction method and an application thereof, the structure of the hapten and the artificial antigen is shown as a formula (1): R1 =-COOH, R2 = R3 = H; r2 =-COOH, R1 = R3 = H; r3 is-COOH, and R1 is equal to R2 and H is equal to H. The invention discloses a novel sulfonamide immune hapten and a construction method of an artificial antigen of the novel sulfonamide immune hapten for the first time, and successfully obtains a specific antibody with high titer, high sensitivity and broad-spectrum recognition capability on the basis of immune animals of the artificial antigen.

The utility model relates to the technical field of medicine detection, in particular to a medicine detection device with a grinding function. The medicine detection device with the grinding function comprises a detection box and a box door installed on the front side of the detection box. The controller is mounted at the top end of the detection box; the first electric rod is embedded in one side of an inner cavity of the detection box, a rolling assembly is installed at the telescopic end of the first electric rod, an insertion rod is fixedly installed at the position, below the rolling assembly, in the detection box, and a medicine containing assembly matched with the rolling assembly is inserted into the insertion rod; the second electric rod is installed at the bottom end of the detection box, and a medicine dissolving assembly is fixedly installed at the telescopic end of the second electric rod; the third electric rod is embedded in the other side of the inner cavity of the detection box, and a detection probe is fixedly installed at the telescopic end of the third electric rod and electrically connected with the controller. The medicine detection device with the grinding function has the advantages that medicine grinding is convenient and fine, and water solubility detection is facilitated.

The embodiment of the invention relates to the technical field of drug detection, in particular to an anti-CD38 antibody drug receptor occupation detection method and system, a peripheral blood sample is used for preparing a single-cell suspension, the single-cell suspension is divided into a TEST tube and an FMO tube, a cell surface antibody and an RO detection antibody are added into the TEST tube, only the cell surface antibody is added into the FMO tube, and only the RO detection antibody is added into the FMO tube. The TEST tube and the FMO tube are subjected to sample detection analysis, so that the average fluorescence intensity of RO detection antibodies of the TEST tube and the FMO tube is obtained. And performing RO detection analysis by adopting a dissociation method, taking the tubes which are not combined with the medicine as a total receptor group, taking the tubes which are combined with the medicine as a dissociation receptor group, and calculating an RO detection result according to the average fluorescence intensity of the TEST tube and the FMO tube.

The present invention provides a method and a detection device for detecting drugs and their metabolites in hair, which relates to the technical field of drug detection. The present invention collects hair samples, prepares samples with known drug concentration values, collects spectral data through a Raman spectrometer, inputs the spectral data matrix and the response variable matrix, analyzes them, generates a relationship matrix reflecting the correlation between the spectral data matrix and the response variable matrix, and further processes it to generate a weight vector reflecting the weight coefficients of the detection sample concentration at corresponding wavelengths. By analyzing the correlation between the weight vector and the spectral data matrix, a confidence vector that maximally reflects the directional relationship between the response variable matrix Y and the spectral data matrix X is generated, a back-projection matrix is generated after back-projection, and a confidence index is output. By comparing the confidence index with a confidence threshold, it is output whether the concentration detected by the Raman spectrometer in the current environment is valid.

Provided in the present invention is a method for detecting the fingerprint of an analgesic cream for knocks and falls, relating to the technical field of drug detection. The detection method comprises the following steps: preparing a test sample solution; preparing a reference sample solution; and injecting the test sample solution and the reference sample solution into a high performance liquid chromatograph, and recording the chromatogram. The method has the advantages of simplicity, stability, high precision, and good reproducibility. The method can conduct multicomponent fingerprint research on the analgesic cream for knocks and falls, and perform attribute research and similarity evaluation on main chromatographic peaks. The method comprehensively reflects the uniformity and stability of the analgesic cream for knocks and falls, and can evaluate, more comprehensively and scientifically, the quality of the analgesic cream for knocks and falls, thereby ensuring the quality and efficacy of the product.

The invention relates to the technical field of medicine feeding devices, and discloses a quantitative medicine feeding device for mouse experiments, which comprises a box body, a collecting tank body is placed at the bottom end of the box body, quantitative feeders are symmetrically mounted and connected to the inner wall of the box body, and a glass sealing plate is fixedly connected to the top end of the box body; the disinfection mechanism horizontally slides on the inner wall of the box body and comprises a bottom plate, holes are uniformly formed in the middle of the bottom plate, sliding openings are formed in the two sides of the bottom plate, L-shaped plates are slidably connected into the sliding openings, and a shell is fixedly connected to the tops of the L-shaped plates. According to the device, the disinfection mechanism is mechanically driven to conduct automatic intermittent cleaning in the bottom plate, the disinfection assembly is matched to abut against the bottom plate, residual urine and the like on the bottom plate can be cleaned while the bottom plate is disinfected and killed, due to the movable design of the shell, the pressure of mice can be reduced, and the mouse disinfection effect is improved. Extra influence factors under quantitative drug feeding of the mice are reduced, and the drug detection and comparison effect is guaranteed.

The invention belongs to the technical field of medicine detection, and discloses a Raman spectrum-based traditional Chinese medicine decoction piece total flavone content detection method, which comprises: obtaining standard spectrum data of a standard flavone compound and characteristic spectrum data of a traditional Chinese medicine decoction piece sample; performing correlation degree evaluation according to the characteristic spectrum data and the standard spectrum data to obtain a flavone characteristic peak recognition matrix of each traditional Chinese medicine decoction piece sample; screening an effective characteristic wave band based on the flavone characteristic peak identification matrix; according to the spectral absorption intensity and the structure information of the effective characteristic wave band of each traditional Chinese medicine decoction piece sample, compiling a flavone quantitative characterization vector of the sample; detecting the content of total flavonoids in the traditional Chinese medicine decoction pieces on the basis of the flavone quantitative characterization vectors; the detection accuracy of the total flavone content of the traditional Chinese medicine decoction pieces is greatly improved.

The saliva detection piece comprises a detection tube with a closed bottom, a collecting tube is connected to the open end of the detection tube, an annular detection piece is arranged in the detection tube and connected to a rotating disc, and an observation window is formed in the position, corresponding to the detection piece, of the detection tube; one end face of the turntable is connected with a rotating shaft, the rotating shaft penetrates through the detection tube and then is connected with a rotating handle, the outer side of the rotating handle is sleeved with a limiting piece, one end of the limiting piece is connected to the closed end of the detection tube, the outer wall of the rotating handle is connected with a limiting clamping ring, and the end of the rotating handle is clamped in the limiting piece through the limiting clamping ring. According to the utility model, the detection piece can be quickly covered with saliva by only enabling a subject to spit saliva into the collecting tube, keeping the detection tube inclined and rotating the rotating handle, and the detection result can be quickly seen through the observation window and is immediately compared and analyzed, so that the suspect who takes drugs can be quickly, accurately and conveniently detected.

The invention relates to a pulmonary fibrosis model and a construction method and application thereof. The pulmonary fibrosis model provided by the invention is good in stability and controllability and high in maturity, can better simulate functions and structural characteristics of pulmonary alveoli-capillary barriers, has the advantage of high simulation, and has a good application prospect in the aspects of disease simulation, drug detection, screening, evaluation, research and development and the like.

The invention discloses a bladder cancer lymph node metastasis derived tumor organoid culture medium, a culture method and a rapid drug detection method, and belongs to the technical field of organoid and cell culture. The method comprises the following steps: culturing a bladder cancer lymph node metastasis-derived tumor organoid by using a bladder cancer lymph node metastasis-derived tumor organoid culture medium, immersing matrigel drops in the culture medium, and obtaining the bladder cancer lymph node metastasis-derived tumor organoid after the cultured organoid grows to 100-150 microns, to-be-detected drugs with different concentrations are directly added into the cell pore plate inoculated with the tumor organoids, the influence of the to-be-detected drugs on cell growth or metabolism is analyzed through a full-view bright-field imaging method, and the detection result of the drug for treating bladder cancer lymph node metastasis is obtained. Compared with a traditional ENR organ-like culture medium, the method has the advantages that the effect is better, and the cost is lower; the kit is used for screening medicines for bladder cancer lymph node metastasis, is simple and efficient, and can be used for accurately detecting medicines for efficiently killing bladder cancer lymph node metastasis cells.

The invention relates to a drug sample grinding device for drug detection, which is applied to the field of drug grinding technology. The device comprises a base, a grinding cup, a device cover, a lifting and rotating module, a grinding pestle, a vertical shaft, a code collision block, a spring and a magnetic circle block. The device puts drug particles into the grinding cup, and then the grinding pestle is extended into the grinding cup. The lifting and rotating module drives the grinding pestle to lift and rotate, so that the grinding pestle crushes and grinds the drug in the grinding cup. The grinding pestle repeatedly lifts and lowers during the grinding process, and the magnetic force of the magnetic circle block and the code collision block is used to repel each other. When the grinding pestle moves downward, the code collision block rises in the opposite direction. When the grinding pestle moves upward, the code collision block descends and hits the grinding pestle, so that the drug attached to the grinding pestle is shaken off, and the drug powder is shaken back to the grinding cup for redistribution, so that multiple grinding operations of the drug are convenient, the uniform grinding effect of the drug is effectively improved, and the grinding time of the drug is effectively reduced.

The invention belongs to the technical field of drug detection, and particularly relates to a test strip for detecting O6-monoacetylmorphine and methamphetamine and a detection methamphetamine, the test strip comprises a gold-labeled pad, the surface of the gold-labeled pad is sprayed with an O6-monoacetylmorphine and methamphetamine antibody-three-dimensional dendritic gold nanoparticle conjugate, a mixture of an O6-monoacetylmorphine coating antigen and time-resolved europium fluorescent microsphere-BSA (Bovine Serum Albumin) is sprayed on the NC membrane to serve as a T1 line, a mixture of a methylamphetamine coating antigen and time-resolved europium fluorescent microsphere-BSA is sprayed on the NC membrane to serve as a T2 line, and a mixture of a goat anti-mouse secondary antibody and time-resolved europium fluorescent microsphere-BSA is sprayed on the NC membrane to serve as a C line; a to-be-detected sample is dropped on the surface of the sample pad on the surface of the test strip main body for detection. The O6-monoacetylmorphine and methamphetamine detection efficiency, sensitivity and specificity are high, the operation is simple and convenient, and the method can be applied to combined determination of two drugs in a biological detection material.

The invention relates to the technical field of biological medicine, in particular to a drug screening model construction method based on airway organs, a model and application. The construction method of the drug screening model of the airway organoid comprises the steps of obtaining 2D airway epithelial cells, culturing the airway organoid, establishing an airway organoid inflammation model and / or establishing an airway organoid mucus secretion / goblet cell metaplasia model. Wherein the model is established according to the culture mode of the airway organ in cooperation with a specific stimulant for proliferation and differentiation. Compared with a traditional animal model, the organ-like model is low in cost and short in construction period, can better simulate the pathological process, can realize airway organ-like culture and establishment of an inflammation model and a mucus secretion / goblet cell metaplasia model at the same time, and has a good application prospect in drug detection of asthma and COPD diseases.

The invention discloses a method for detecting the content of effective components in a nerofloxacin malate capsule, and relates to the technical field of medicine detection and analysis, and the detection method comprises the following step: detecting the content of the effective components in the nerofloxacin malate capsule by adopting a high performance liquid chromatography. The invention provides the method for detecting the content of the effective components in the nerofloxacin malic acid capsule for the first time, and the inventor finds that the interference of non-effective components such as impurities and auxiliary materials in the nerofloxacin malic acid capsule can be effectively avoided by selecting a specific chromatographic column and a specific mobile phase for isocratic elution; the accuracy of detecting the content of the effective components in the nerofloxacin malic acid capsule is improved, the accurate quantification of the content of the nerofloxacin malic acid capsule is realized, and the blank in the prior art is filled.

According to the automatic production device for the plaster patches, single adsorbed patches are placed on the conveying belt through the patch conveying mechanism, the dripping time and the dripping amount of medicine are regulated and controlled through the medicine spraying mechanism, packaging of the surfaces of the patches through transparent adhesive tape is achieved through the patch packaging mechanism, the medicine extrusion range is limited through a sleeve, and the plaster patches are automatically produced. Image acquisition of the plaster patches and rejection of unqualified products are realized through the medicine detection mechanism, so that automatic production and detection of the quality of the plaster patches are completed. The device has the characteristics of high plaster pasting quality, high production speed, simple structure, reliable operation and the like.

The invention provides a preparation method and drug detection application of ZIF-8-Fe metal organic framework nano-enzyme, the ZIF-8-Fe metal organic framework nano-enzyme is prepared by adopting a heating reflux method, and peroxidase-like activity and drug detection application of the ZIF-8-Fe metal organic framework nano-enzyme are researched. In the presence of H2O2, the material can catalytically oxidize TMB to generate a blue product and shows good peroxidase activity, and through optimization experiments, proper reaction temperature and pH buffer system and optimal concentrations of ZIF-8-Fe, TMB and H2O2 are determined. Kinetic analysis shows that the affinity of TMB is superior to that of natural HRP, and a colorimetric detection method is established based on the inhibition effect of TC on the catalytic reaction. The method disclosed by the invention is reasonable in linear range, relatively low in detection limit and good in sample recovery rate and relative standard deviation performance, can be used for detecting tetracycline tablets, and shows a potential application value of the ZIF-8-Fe nano-enzyme in the field of drug detection.

The invention discloses drug residue detection equipment for aquatic product quality safety, and relates to the technical field of aquatic product drug detection.The drug residue detection equipment comprises a sampling mechanism and a pressing plate installed at the top of the sampling mechanism, a detection mechanism is arranged at the bottom of the sampling mechanism, and a detection beaker is arranged in the detection mechanism; the sampling mechanism comprises a first lifting support, a first lifting motor is fixedly mounted at the top of the first lifting support, the output end of the first lifting motor is fixedly connected with a first lifting threaded rod, and the outer side of the first lifting threaded rod is in threaded connection with a first lifting threaded sleeve; a lifting limiting block is fixedly installed on the outer side of the first lifting threaded sleeve, after liquid sampling is conducted through a heating ring, a solution can be heated and evaporated, steam is discharged through a through hole, and therefore the concentration of the solution can be increased, medicine residues can be conveniently detected, and through rotation of a fan-shaped cutting knife and a cutting sleeve, the medicine residues can be conveniently detected. And aquatic products can be conveniently cut.

The invention discloses a screening method for potential quality difference markers of Mongolian medicine Darbibu-5, and relates to the technical field of medicine detection.The screening method specifically comprises the following steps that S1, the overall quality of Darbibu-5 is evaluated from the macroscopic perspective by adopting a fingerprint spectrum technology, and quality difference component groups are screened in combination with a pattern recognition method; s2, on the basis of a serum pharmaceutical chemistry method and an HPLC-Q-Exactive MS technology, comparing the difference between the in-vitro inherent chemical components and the serum migration components of the Darbibu-5 by adopting an analysis process of spectrum library retrieval and mass spectrum recognition, and further analyzing a Darbibu-5 serum migration component group; and S3, determining the potential quality difference marker of Darbibu-5 based on the common components of the quality difference component group and the serum migration component group. The screening strategy not only pays attention to the overall quality of the preparation, but also considers in-vivo functional components, the quality of the preparation can be analyzed objectively, systematically and comprehensively, and a potential quality difference marker is confirmed by adopting a quality difference component group-serum migration component group integration strategy.