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Drug detection kit based on bicompective immunochromatographic method and preparation technology thereof

A technology for detection kits and immunochromatography, applied in measuring devices, analytical materials, instruments, etc., can solve the problems of incomplete binding, short specific reaction time, and affecting the sensitivity of detection reagents, achieving low cost and improving sensitivity Effect

Inactive Publication Date: 2017-12-15
BOZHOU CITY THE NEW HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in actual production, the sensitivity of the detection kit is affected by various factors: (1) the level of reaction sensitivity depends on the specific reaction of the antigen and antibody used and the modification of the antibody; Short reaction time results in incomplete binding, which affects the sensitivity of the detection reagent

Method used

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  • Drug detection kit based on bicompective immunochromatographic method and preparation technology thereof
  • Drug detection kit based on bicompective immunochromatographic method and preparation technology thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A cannabis (THC) detection kit, comprising a test paper for detecting cannabis and a supporting plastic box, the test paper is composed of a sample pad, a colloidal gold binding pad, a fluorescent microsphere binding pad, a nitrocellulose membrane, an absorbent paper The second lap is pasted on a PVC plastic sheet to form; the colloidal gold binding pad is coated with colloidal gold-labeled THC rabbit IgG antibody, and the fluorescent microsphere binding pad is coated with fluorescent microsphere-labeled THC rabbit IgG antibody; The nitrocellulose membrane includes a detection area (T line) coated with THC antigen and a quality control area (C line) coated with the second antibody goat anti-rabbit polyclonal antibody, such as figure 2 shown.

[0042] Wherein, the preparation of the colloidal gold bonding pad applies reduction technology, and the specific steps are:

[0043] S1: Preparation of colloidal gold particles, the high-valent gold in chloroauric acid is reduce...

Embodiment 2

[0054] A preparation process for cannabis (THC) detection kit, the steps are as follows:

[0055] Step 1: Fix the THC rabbit IgG antibody-colloidal gold marker on the colloidal gold binding pad with a gold spray machine; fix the THC rabbit IgG antibody-fluorescent microsphere marker on the fluorescent microsphere binding pad;

[0056] Step 2: Spot the THC antigen and goat anti-rabbit IgG polyclonal antibody on the detection area and the control area of ​​the nitrocellulose membrane with a film spotting machine, and dry it sufficiently to make the nitrocellulose membrane firmly adsorb the raw materials;

[0057] Step 4: Assemble the PVC plastic sheet according to the production requirements, tear off the covering paper where the absorbent paper is pasted on the PVC plastic sheet, align and paste the absorbent paper along the upper edge; tear off the covering paper where the gold standard pad and sample pad are pasted, and combine the fluorescent microspheres The pad (4mm) is pr...

Embodiment 3

[0059] A kind of ecstasy (methylenedioxyamphetamine) detection kit, including the test paper used to detect marijuana and the supporting plastic box used, it is characterized in that, described test paper is made of sample pad, colloidal gold binding pad, fluorescence The microsphere binding pad, nitrocellulose membrane, and absorbent paper are sequentially lapped and pasted on a PVC plastic sheet; the colloidal gold binding pad is coated with colloidal gold-labeled methylenedioxyamphetamine rabbit IgG antibody , the fluorescent microsphere binding pad is coated with fluorescent microsphere-labeled MDMA rabbit IgG antibody; the nitrocellulose membrane includes MDMA coated with MDMA The detection area (T line) of the bright antigen and the quality control area (C line) coated with the secondary antibody goat anti-rabbit polyclonal antibody, such as figure 2 shown.

[0060] Wherein, the preparation of the colloidal gold bonding pad applies reduction technology, and the specifi...

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Abstract

The invention provides a detection kit applied to colloidal gold competition law and a preparation technology thereof. According to the preparation technology, an antigen-antibody reaction with high degree of specificity is combined with an immune colloidal gold chromatography technique, the preparation technology is improved on the basis of the prior art, fluorescent microspheres are introduced to compete together with colloidal gold marked antibodies for antigens of materials to be detected in a sample, and the fluorescent materials are not visible to naked eyes, so that the sensitivity of the competition law colloidal god detection kit is improved.

Description

technical field [0001] The invention relates to the technical field of biology and new medicine, in particular to a drug detection kit based on double competitive immunochromatography and its preparation process, which is used in drug rehabilitation centers, hospitals, military conscription, high-risk population censuses, special industries and physical examinations for recruitment Screening at work and food inspection by health and epidemic prevention departments. Background technique [0002] At present, the gold standard method is mainly used for urine testing in drug testing, which is characterized by fast, convenient, portable, and high accuracy. The commonly used methods for the determination of single drugs in urine are: monoclonal antibody monolithic qualitative method, thin-layer chromatography, gas chromatography, and gas-mass spectrometry detection method, but these methods exist to varying degrees. Cross-reaction, many interference factors and complex pre-treatm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/533
CPCG01N33/558G01N33/533
Inventor 熊良钟王梓光熊清爵阮志燕孙全峰耿武松蒋磊李军史学礼王振苏新国
Owner BOZHOU CITY THE NEW HEALTH TECH CO LTD
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