Salbutamol drug detection colloidal gold test paper card and detection method

A technology of colloidal gold test paper and salbutamol, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of high degree of instrumentation, high detection cost, complicated sample processing, etc., and achieves high sensitivity, short detection time, and improved detection efficiency. Effect

Active Publication Date: 2013-04-03
BEIJING KWINBON BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the detection methods for salbutamol residues mainly include high performance liquid chromatography, liquid chromatography-mass spectrometry, gas chromatography, capillary zone electrophoresis, immunoassay and other detection methods. The instrument detection method has high detection accuracy. However, it is not suitable for the detection of a large number of samples due to its high degree of instrumentation, complicated sample processing, and high cost of detection. The immunological detection method is simple, fast, and sensitive, and can detect most samples at the same time. It is an ideal rapid screening method. means

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  • Salbutamol drug detection colloidal gold test paper card and detection method
  • Salbutamol drug detection colloidal gold test paper card and detection method
  • Salbutamol drug detection colloidal gold test paper card and detection method

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Experimental program
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Effect test

Embodiment 1

[0057] The preparation of embodiment one salbutamol colloidal gold test paper card

[0058] 1. Preparation of Salbutamol Hapten

[0059] (1) Take 1-3g salbutamol, dissolve in 20-50ml DMF, then add 10% molar equivalent of TEMPO and 10% molar equivalent of tetrabutylammonium chloride, 20-50ml 0.5M NaHCO 3 Aqueous solution, stirred at room temperature for 30 minutes, added 1.1-1.5 molar equivalents of NCS, reacted at room temperature for 2-5 hours, removed most of the solvent, extracted with ethyl acetate, washed, dried, and purified by column chromatography after removing the solvent to obtain white Solid, salbutamol monooxide.

[0060] (2) Dissolve 1-2g of albuterol oxide obtained in step (1) into 20-30ml of DMF, add 2-5 molar equivalents of ethylenediamine, react at room temperature to 100°C for 5-20 hours, remove the solvent, and dissolve in ethanol-water Recrystallized to obtain a white solid, which is the ethylenediamine condensation product of salbutamol monooxide, namel...

Embodiment 2

[0094] The detection of salbutamol residue in the sample of embodiment two

[0095] 1. Sample pretreatment

[0096] Urine Samples: Urine samples must be collected in clean, dry plastic urine cups or glass containers that do not contain any preservatives. If it cannot be sent for inspection in time, the urine sample can be stored at 2-8°C for 4 hours. For long-term storage, it should be frozen at -20°C. Repeated freezing and thawing is strictly prohibited. The urine sample needs to be warmed to room temperature before testing. If the urine sample is turbid, it needs to be centrifuged at 3000rpm or more for 5 minutes before testing.

[0097] Pork, chicken, fish, shrimp and other tissues: take the adipose tissue sample and homogenize it in a homogenizer for 1min (10000rpm), weigh a certain amount (1.0g) of the homogenized tissue sample into a 4ml centrifuge tube, add the sample extract 200μl, vortex for 1min; put in 80-100℃ water bath for 15min, take out and cool to room temper...

Embodiment 3

[0105] Example three sample detection example

[0106] 1. Detection limit test

[0107] Take blank pig urine, pork, chicken, fish, shrimp, and feed samples respectively, add albuterol to the blank pig urine samples to a final concentration of 3, 5, and 10 μg / L, and add albuterol to the blank pork, chicken, fish, and shrimp samples To the final concentration of 10, 20, and 40 μg / kg, add albuterol to the blank feed sample to the final concentration of 15, 30, and 60 μg / kg, take the colloidal gold test paper card for detection, and repeat the measurement three times for each sample.

[0108] When the colloidal gold test paper card was used to detect the pig urine sample, when the added concentration of albuterol in the pig urine sample was 3 μg / L, two red lines visible to the naked eye were displayed on the test paper card, which was negative; when the added concentration of salbutamol in the pig urine sample When it was 5 or 10 μg / L, the quality control area of ​​the test paper...

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Abstract

The present invention provides a salbutamol drug detection colloidal gold test paper card, which comprises a sample absorption pad, a conjugate releasing pad, a reaction membrane, a water absorption pad and a backing, wherein the reaction membrane is provided with a detection line formed by coating a salbutamol-carrier protein conjugate and a quality control line formed by coating goat anti-mouse anti-antibody, and the conjugate releasing pad is coated with a salbutamol monoclonal antibody-colloidal gold marker. The present invention further provides a method for adopting the salbutamol colloidal gold test paper card to detect salbutamol drugs. The salbutamol drug detection colloidal gold test paper card can be used for detection of salbutamol drug residues in urine, pork, chicken, fish, shrimp and feed samples, has characteristics of simple operation, high sensitivity, rapid detection and low cost, and is suitable for screening and on-site monitoring of a large number of samples.

Description

technical field [0001] The invention relates to a colloidal gold test paper card for detecting albuterol and a detection method. Background technique [0002] Salbutamol is a potent selective β-receptor stimulant drug, which is commonly used clinically to treat respiratory diseases such as bronchial asthma, asthmatic bronchitis, and bronchospasm in patients with emphysema. Therefore, the medicine can increase lean meat rate, reduce fat deposition and promote animal growth, and is illegally used as a breeding accelerator by some animal husbandry enterprises. Its residual amount can cause muscle tremors, palpitations, nervousness, headache, dizziness, nausea, vomiting, fever, trembling and other symptoms in the human body, which is extremely harmful to the health of consumers. The List of Compounds clearly stipulates that albuterol and other β-stimulants are prohibited for all uses, and all food animals are prohibited. [0003] At present, the detection methods for salbutamo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/531
Inventor 冯才伟罗晓琴朱亮亮崔海峰汤庆彩崔彦虎张荃其他发明人请求不公开姓名
Owner BEIJING KWINBON BIOTECH
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