11results about How to "Lower detection threshold" patented technology

The invention discloses a logic vulnerability detection method and device, equipment and a medium, and relates to the technical field of computers. Comprising the following steps: acquiring information exchange traffic which is generated by a target interface and is acquired by a traffic acquisition system from a detector dispatching center, and calling a preset retransmission rule from a rule center to generate a retransmission request for the information exchange traffic; obtaining a current response result corresponding to the resending request, and calling a preset judgment rule from the rule center to judge the current response result so as to obtain a corresponding judgment result; and returning the judgment result to the detector scheduling center to complete the logic vulnerability detection process. Therefore, the logic vulnerability detection threshold is reduced, and the generalization and accuracy of logic vulnerability detection are improved.

The application discloses a kind of cultivation of smell and headspace analysis combination's odd nan hand string nondestructive identification method, it is related to precious timber true and false identification and quality detection technical field, including following step A: sample pool data model establishment: a1, sample selection: collect the cultivation of different producing areas, gram weight, shape and size odd nan hand string genuine sample not less than 200;Collect by high pressure injection paste, press oil, bubble medicine, high throwing, immersion paste smearing and non-lignum aquilariae imitated fake sample not less than 200;A2, electronic nose data acquisition: using electronic nose to the sample carries out smell data acquisition, acquisition condition includes;By being provided with electronic nose data acquisition and key sensor screening, the whole identification process does not need to be destructively treated to sample any cutting, grinding etc., completely maintain the integrity and value of hand string, and electronic nose detection can be completed within 1 hour, headspace mass spectrum analysis as supplementary means can be completed within 3 hours, improve detection efficiency.

The application provides a platelet non-invasive testing device, which comprises a shell, a hitting assembly and a triggering assembly. The shell has a first wall; the hitting assembly is movably arranged in the shell along a first direction, and the hitting assembly comprises a hitting part for hitting the skin; the triggering assembly is mounted on the shell and is in transmission connection with the hitting assembly; the triggering assembly has a triggering state and a reset state relative to the hitting part; when the triggering assembly is in the triggering state and the reset state respectively, the hitting part is located on the same side of the first wall; along the first direction, the hitting part has a first distance and a second distance from the first wall when the triggering assembly is in the triggering state and the reset state respectively, and the first distance is greater than the second distance. The platelet non-invasive testing device can hit the skin, detect and evaluate the function of platelets through ecchymosis generated on the surface of the skin, can avoid the disadvantages of blood sampling, and thus can reduce the difficulty of detecting the function of platelets of patients who are difficult to take blood and suffer from blood diseases.

The invention provides a personalized health intervention system based on a braingut axis theory, and the specific operation comprises the following steps: S1, household standardized sampling and low-temperature transportation: providing a standardized household sampling suite, enabling a user to collect a feces sample, carrying out compliance storage and transportation, and transporting the sample to a detection point position; s2, performing high-throughput sequencing analysis on the intestinal flora, namely receiving the excrement sample acquired by the household sampling module, performing sequencing analysis on the intestinal microbiome in the sample through a high-throughput sequencing technology, and outputting composition, abundance and function annotation data of the intestinal flora; and S3, user emotion and sleep phenotype data acquisition: acquiring emotion and sleep related phenotype data autonomously reported by the user. According to the invention, convenient collection and stable transportation of intestinal flora samples are realized through the standardized household sampling suite, a user does not need to go to a professional institution, the detection threshold is reduced, and the user participation degree is improved.

The invention discloses a biomarker for gastric cancer assessment and application thereof, and belongs to the technical field of biological medicine. The biomarker comprises at least one of the following florae: a Metuquise flora, an MEblack flora, an MEpin flora, an MEblue flora, an MEylow flora, an MEtan flora, and an MEgreenylow flora. The invention further discloses a method for preparing the biomarker. The biomarker comprises at least one of the following florae: the Metuquise flora, the MEblack flora, the MEpin flora, the MEblue flora, the MEylow flora, the MEtan flora, and the MEgreenylow flora. The biomarker is screened from fecal intestinal flora, the biomarker is developed on the basis of a microorganism sequencing sequence, dependence of traditional microorganism related inventions on a database is avoided, and the influence of annotation of microorganism classification status on a result is reduced. The method provided by the invention can realize self-test of people at home, reduces the detection threshold, improves the detection convenience and efficiency, can be widely applied to noninvasive GC early screening, and has great application potential.

The invention relates to the technical field of molasses sugar content detection, and discloses a method for detecting molasses sugar content by combining microbial fermentation with a refractometer, and the method comprises the following steps: S1, preparing reagent materials; s2, diluting the permeophilic yeast suspension and sterile normal saline according to a ratio of 1: 10; s3, treating a molasses sample to be detected; s4, setting a measuring pipe; s5, setting a control tube; and S6, determination: calculating the sugar content of the molasses according to a formula X sugar content = (X0-X1) * V / m. Compared with a traditional polysaccharide detection kit method, the method has the advantages that the detection time is greatly shortened, long enzymolysis time, standard curve making and OD value detection time are not needed, only simple instruments such as a refractometer are used, and the method for detecting the sugar content of the molasses is extremely low in cost and relatively high in efficiency, and has a wide application prospect. The method has excellent accuracy and stability, is low in detection threshold, and can be widely applied to molasses production, transaction and processing industries.

This invention discloses a detection kit technology, specifically a detection kit and method for novoarginine, comprising a universal sample processing solution, a novoarginine-specific probe, fluorescently labeled streptavidin, washing solution, chromogenic substrate solution, stop solution, novoarginine standard, and a reaction microplate. The universal sample processing solution is composed of acetonitrile, potassium dihydrogen phosphate buffer, and disodium ethylenediaminetetraacetate in a volume ratio of 3:6:1, with the potassium dihydrogen phosphate buffer concentration being 0.1 mol / L and the disodium ethylenediaminetetraacetate concentration being 0.05 mol / L. The novoarginine-specific probe is a biotin-labeled novoarginine monoclonal antibody at a concentration of 1 mg / mL. The surface of the reaction microplate is coated with novoarginine hapten. This invention, by developing a universal sample processing solution adaptable to different matrix samples, eliminates the need for customized pretreatment procedures, significantly improving the versatility of the kit and solving the cumbersome problem of requiring separate processing for different samples.

The invention provides a preparation method and application of a silver nano-cluster probe and a silver nano-cluster assembly, and belongs to the technical field of nucleic acid probes, the silver nano-cluster probe comprises the silver nano-cluster assembly and a signal conversion sequence, the silver nano-cluster assembly comprises a template chain and a silver nano-cluster combined on the template chain, and the signal conversion sequence is a signal conversion sequence. The template chain comprises a nucleic acid aptamer sequence and a silver nano-cluster nucleation sequence. The ratio type fluorescent probe is constructed by utilizing the specific recognition effect of the nucleic acid aptamer on viable bacteria and combining the regulation and control of the signal conversion sequence on the fluorescence of the silver nanocluster, the probe can effectively eliminate environmental interference and specifically distinguish dead and viable bacteria, the detection result is visible under an ultraviolet lamp by naked eyes, and the method is simple and convenient to operate. The method is suitable for rapid and accurate detection of specific viable bacteria in a complex food matrix.

The invention provides a non-nucleic acid target rapid detection method based on magnetic separation-CRISPR signal amplification, a kit and application, and belongs to the technical field of detection. The method specifically comprises the following steps: S1, preparing an antibody modified magnetic nanoparticle solution; s2, modifying the antigen / antigen analogue and single-stranded DNA to the surface of a gold nanoparticle to obtain a gold nanoparticle compound solution; s3, preparing the magnetic nanoparticle solution, the gold nanoparticle compound solution and the non-nucleic acid target sample solution to be detected into an MNPs-Ab compound solution; s4, mixing a Cas12a protein solution, a new England buffer solution, a crRNA solution, an RNA enzyme inhibitor solution and a fluorescent probe solution to obtain a system complex solution; and S5, carrying out mixed reaction on the MNPs-Ab compound solution and a system compound solution, and finally carrying out fluorescence detection. The method is high in sensitivity and short in detection time, and has the capability of quantitatively detecting non-nucleic acid targets, so that the method has a good development prospect.

The application discloses a HPV virus detection kit, which improves the problems of traditional HPV detection, such as the need of expensive instruments, complicated operation and long detection time, by adopting LAMP technology and visual detection design; the kit contains a freeze-drying tube, a liquid reaction tube and a sample lysis tube, LAMP primer groups in the freeze-drying tube are combined with Bst DNA polymerase to realize constant temperature amplification; when the MOPS buffer microspheres in the liquid reaction tube release components to maintain the reaction environment at pH change, the accuracy and stability of the chromogenic accuracy of neutral red are protected, and the neutral red is used as an indicator to intuitively present the result through color (orange yellow negative, red positive); after the sample is mixed with the lysis solution and heated, the reaction system is added at 60 DEG C constant temperature for 30 min, and naked eye judgment can be carried out, without the need of a thermal cycler and other equipment, so that the HPV screening is effectively promoted in the popularization and application of primary medical institutions.

The present invention discloses a vehicle-mounted Doppler radar detection system, a detection array and a detection method, which are used to detect the current health status of locomotives, tracks and roadbeds and their long-term change trends. The detection system includes a radar transmitting and receiving module and a main detection module. The radar transmitting and receiving module can be fixed to a rail vehicle and move along the track with the rail vehicle, and is used to transmit a radar beam to the track and receive a radar beam reflection signal during movement along the track. The detection module is used to process the radar beam reflection signal to obtain the Doppler characteristic information of the radar beam reflection signal according to the Doppler effect of each radar reflection beam, and judge the state of the detection target according to the Doppler characteristic information. The present invention installs the transmitting and receiving module of the Doppler radar on the rail vehicle and moves with the vehicle, without the need for a drone to follow the detection, thereby lowering the detection threshold, being easy to use and having high detection efficiency.