202 results about "Amino Acid Injection" patented technology

The invention relates to the technical field of clinical medicines, and particularly relates to a cell preservation solution and a preparation method and applications thereof. The cell preservation solution comprises albumin, glucose, vitamin C and basic preservation liquid, wherein the basic preservation liquid is a mixture of compound electrolyte injection and compound amino acid injection. The cell preservation solution disclosed by the invention achieves a good maintaining effect on the viability and morphology of more than two kinds of seed cells, and can preserve the seed cells for a long time, and maintain the biological characteristics of the seed cells, thereby significantly improving the therapeutic effect of the seed cells.

The invention discloses compound amino acid injecta, which contains arginine hydrochloride, histidine monohydrochloride, leucine, isoleucine, lysine hydrochloride and the like; meanwhile, the invention further discloses a preparation method of the compound amino acid injecta (18AA); in the event of ensuring the product quality, the compound amino acid injecta has the characteristics of saving energy and enhancing efficacy; by means of repetitive verification on precision, detection limit, quantitation limit, linear range and the like, a simple, convenient, rapid and reliable method with cleaning verification is ensured; furthermore, the residual quantity of antioxygen, namely sodium hydrogensulfite, in the compound amino acid injecta and sulphate ion generated by degrading sodium hydrogensulfite are detected according to an ion chromatography principle, thus, the amount of the antioxygen charged in the raw material proportioning can be accurately measured, and the stability of the raw material proportioning process is judged according to the measurement result; and a detection method of the compound amino acid injecta provided by the invention is an online derivative amino acid content measuring method, the speed is rapid, and the method is applied to large-scale detection.

The invention discloses a pharmaceutical composition containing 18 amino acids. The pharmaceutical composition is characterized in that a compound amino acid injection (18AA-V) with varied concentration is prepared by using the following components according to the following ratios of parts by weight: 2.89 of arginine hydrochloride, 2.46 of histidine hydrochloride, 3.79 of leucine, 1.70 of isoleucine, 3.33 of lysine hydrochloride, 2.83 of phenylalanine, 1.97 of threonine, 1.36 of valine, 1.06 of methionine, 0.39 of tryptophan, 3.24 of glycine, 1.88 of alanine, 1.00 of proline, 0.11 of tyrosine, 0.67 of serine, 0.44 of cysteine hydrochloride, 1.15 of aspartic acid, 1.97 of glutamic acid, 50 of xylitol, 0.10 to 0.30 of citric acid and injection water with proper amount. The pharmaceutical composition does not contain a sulfite antioxidant so that the pharmaceutical composition is clinically used in a safer manner. After an accelerated test and a quality test, results show that the pharmaceutical composition is as stable as or more stable than like products (18AA-V) which are sold in the markets and contain sulfites.

The invention discloses an injection composition packaged in a three-cavity bag. The three cavities separated by diaphragm are respectively filled with fattiness milk injection, compound amino acid injection, and glucose injection. The using method is crimping each cavity in turns, or divulging the isolating bar in each cavity to mix the injections for venoclysis so as to avoid the venture of microorganism and particulates pollution in traditional mix processing. The mix processing can be carried out under any environmental condition which qualifies the small hospital without aseptic preparation condition to use the nutrition, highly reduce the untoward reaction such as transfusion heater response, sanguis infection, and blood poisoning due to traditional mix processing.

The invention discloses a pharmaceutical composition containing 18 amino acids. The pharmaceutical composition is characterized in that a compound amino acid injection (18AA-II) with varied concentration is prepared by using the following components according to the following ratios of parts by weight: 1.50 of aspartic acid, 2.50 of glutamic acid, 1.90 of serine, 3.00 of histidine, 3.50 of glycine, 2.50 of threonine, 7.20 of alanine, 4.90 of arginine, 0.20 of tyrosine, 0.20 of cystine, 3.20 of valine, 2.50 of methionine, 0.85 of tryptophan, 3.50 of phenylalanine, 2.50 of isoleucine, 3.40 of leucine, 5.50 of lysine acetate, 2.90 of proline, 0.10 of cysteine hydrochloride and 0.20 of lemon acid. The composition does not contain a sulfite antioxidant so that the pharmaceutical composition is clinically used in a safer manner. After an accelerated test, a test result shows that the pharmaceutical composition containing 18 amino acids is as stable as or more stable than like products which are sold in the markets and contain sulfites.

The invention discloses a culture method, application and a combined preparation of a hypoxia mesenchymal stem cell. By adopting an umbilical or placenta mesenchymal stem cell and combining the means of transfusing compound amino acid injection and the like, the vitality, the implantation survival rate and the treating effect of the mesenchymal stem cell in vivo can be improved; and in order to avoid the possibility of conglutination, conglomerating and cell mass embolism of the mesenchymal stem cell in blood vessel, the matching of saline containing heparin during treatment can be carried out by a mode of intravenous injection or intravenous drip. With more than three times of treatment, physical conditions of curee can be remarkably improved and objective indicators such as immunity, blood fat, blood sugar and the like can be remarkably improved.

The invention discloses an amino acid injection, commonly known as propranolol, which relates to a pharmaceutical preparation containing 18 kinds of amino acids. The amino acid injection contains the following components per 1,000 milliliters: isoleucine, leucine, methionine, phenylalanine, threonine, tryptophan, valine, glycine, alanine, glutamic acid, aspartic acid, proline, serine, cystine, lysine hydrochloride or lysine acetate, arginine hydrochloride or arginine, histidine hydrochloride or histidine, tyrosine or acetyl tyrosine, and sodium bisulfite or sodium meta-bisulphite. The osmotic pressure ratio of the amino acid injection is lower than 1.8, which effectively reduces the pain for a patient in infusion; the added quantity of antioxidant is small, which can reduce the clinical adverse reactions; and an infusion bottle body is large, which is convenient to mix liquid in clinical application.

The invention relates to a pharmaceutical composition of alanyl glutamine aseptic frozen-dried powder for injection and compound amino acid injection, particularly a combined application package, comprising alanyl glutamine aseptic frozen-dried powder for injection, water for injection, and compound amino acid injection. When the pharmaceutical composition is used, the alanyl glutamine aseptic frozen-dried powder for injection is dissolved with water for injection, and then is mixed with compatible amino acid injection or transfusion containing amino acid, and intravenous infusion is used. The combined application package disclosed herein avoids the influence to quality caused by quality discrepancy of different factories when the combination is used, and greatly increases the safety of application of patients.

The invention provides a preparation method of a compound amino acid injection. Due to the adoption of vacuumization and nitrogen injection in an injection preparation process, oxygen in preparation equipment and water-soluble dissolved oxygen are removed, and the problems that amino acid is instable and is vulnerable to oxidizing in a solution so as to enable the color of the liquid medicine to turn yellow and the content of the amino acid to be decreased are solved. The preparation method provided by the invention is characterized in that the most insoluble amino acid composition, i.e. cystine, is dissolved by low-concentration alkali liquor independently and then is subjected to batch feeding, other amino acid components are subjected to batch feeding at a time, and multi-group batch feeding or high-temperature batch feeding of each component is not needed; and the preparation method has the advantages of simple and convenient batch feeding way, low preparation temperature, short preparation time and the like, has lower requirement on production facilities, and can be easily operated by production staff.

The present invention is one kind of three-cavity packed injection composition, and has medium/long chain fatty milk injection, compound amino acid injection and glucose injection packed separately in three diaphragm separated cavities. Before intravenous infusion, the medium/long chain fatty milk injection, the compound amino acid injection and the glucose injection are squeezed out and mixed in common operation condition. The injection composition can be used to patient for meeting the requirement in protein and saccharide.

The present invention is one kind of three-cavity packed injection composition, and has structural fatty milk injection, compound amino acid injection and glucose injection packed separately in three diaphragm separated cavities. Before intravenous infusion, the structural fatty milk injection, the compound amino acid injection and the glucose injection are squeezed out and mixed in common operation condition. The injection composition can be used to patient for meeting the requirement in protein and saccharide.

The invention discloses clinical mesenchymal stem cell cryopreservation solution and a preparation method thereof. The preservation solution comprises the following components in percentage by weight:1-5% of autologous plasma lysate mixed liquor, 1-2% of human serum albumin, 0-1% of VC injection, 5-10% of glucose injection, 20-30% of electrolyte-like solution, 20-30% of glucosamine injection, 20-30% of compound amino acid injection, 2-5% of dimethyl sulfoxide and 2.5-5% of trehalose. The prepared cryopreservation solution can be used for deep low-temperature and long-term storage of cells, and can realize long-distance transportation, so that the distribution range of cells is expanded, the long-distance treatment of stem cells is realized, and the cells can be directly returned after recovery so as to reduce the potential safety hazards caused by complicated operations.

The invention mainly relates to a method for preparing compound amino acid injection liquid (20AA), and belongs to the technical field of medicines. The problems that the amino acid is oxidized and degraded and solution color is deepened because the solution is in contact with oxygen for a long time during preparation and filling are solved by vacuumizing and filling nitrogen during preparation of the injection liquid. In the method, the injection liquid is dissolved in batch or in several times according to the dissolution difficulty of amino acid, so that the problem that the indissolvable amino acid crystal separates out during preparation is solved, and the stability and the quality of the amino acid injection liquid are guaranteed to the maximum limit. The method has the advantages of simple operation, scientific and reasonable process, low cost and low requirement on production equipment.

The present invention belongs to one mixed amino acid injection containing 18 kinds of amino acids and is named Lovamin. Its main components includes 18 kinds of amino acids in 8.5 wt% or 11.4 wt%, sulfite in 0.001-0.003 wt% and glacial acetic acid in small amount other than injection water. Its preparation process includes the steps of compounding of injection, filling and disinfecting in a steam sterilizer. Compared with similar product, the present invention has less side effect.

The invention provides a preparation method of a compound amino acid injection. The preparation method comprises the following steps: respectively washing a preparation container and a pipeline with alkaline water, a metal ion chelating agent solution and water for injection; putting the water for injection into a liquid preparation tank, adding sodium hydroxide, and stirring while adding until the sodium hydroxide is dissolved; introducing N2 into the liquid preparation tank, then sequentially adding tyrosine, isoleucine, leucine, lysine hydrochloride, methionine, threonine, valine, alanine,arginine hydrochloride, aspartic acid, glutamic acid, proline, serine, glycine, phenylalanine, histidine hydrochloride, xylitol and cysteine hydrochloride, and stirring and dissolving one by one; continuously introducing N2 into the liquid preparation tank, ensuring that the oxygen content is within 3%, cooling the materials, adding water for injection, adding tryptophan, stirring, dissolving, andsupplementing water to a full amount to obtain a mixed solution; and filtering and sterilizing the mixed solution, injecting the mixed solution into an infusion bottle, capping and sealing the bottle, and performing sterilizing and performing light inspection to obtain the compound amino acid injection.

The invention relates to preparation and an application method of a human peripheral blood stem cell freezing medium. The stem cell freezing medium is prepared from the following components in percentage by volume: 48.2% of hydroxyethyl starch dextran, 2% of compound amino acid injection (18AA-IV), 4.8% of dimethyl sulfoxide and 20% of human serum albumin. The stem cell freezing medium has the characteristics of high viable rate after recovery (greater than 99%) and availability for vein back transfusion. The invention further provides the preparation and the application method of the human peripheral blood stem cell freezing medium. The preparation comprises the following steps: under sterile environment, premixing all the components of the human peripheral blood stem cell freezing medium in sequence, adding a stem cell suspension, mixing uniformly, and packing into a cryopreservation bag for cryopreservation. Stem cells with the freezing medium can be directly placed into a cryogenic refrigerator at -80 DEG C for storage, a programmable cooler is not needed, the operation is simple and convenient, and the use is safe.

The invention discloses a compound amino acid injection for livestock as well as a preparation method and applications thereof. The main components of the injection are 18 amino acids and gamma-aminobutyric acid needed by an animal organism. In the preparation process, metal ions in disodium ethylene diamine tetraacetic acid complexing water are used, and carbon dioxide airflow is introduced in the whole production process, thereby solving the problem that the injection is easy to oxidize and change color and stabilizing the pH value of the reaction system. The invention can timely supplement essential amino acid and non-essential amino acid required during the disease of the animal organism, so as to strengthen the physical ability of animals and shorten the disease treatment time; and the added gamma-aminobutyric acid can reduce the unconscious movement of the animal so as to reduce energy consumption, promote the secretion of the gastric juice of the animal and increase the feed intake. The compound amino acid injection is used for recovery of the animal organism in the disease treatment period and the after-disease recovery period.

The invention relates to the field of medicine preparation and especially relates to a compounded amino acid injection 18AA-II content measurement method. According to the method, when the time of conversion of a buffer solution from B2 to B3 is pushed back by 0.5 min, the time of conversion of a buffer solution from B3 to B4 is pushed back by 0.8 min, and the temperature of a separation column reaches 57 DEG C, a better separation effect between the components is achieved and further separation degree between cystine and valine satisfies the demand on content measurement, and meanwhile, interference on the cystine due to conversion of a mobile phase is reduced, so that accurate quantitative measurement is achieved even under a low content, and finally, accurate measurement of contents of sixteen amino acids in the compounded amino acid injection 18AA-II with an amino acid analyzer can be achieved.

The invention discloses a tissue preserving fluid. The tissue preserving fluid is composed of, by concentration, 1.0-2.5%w/v of poloamer, 1.0-2.5%w/v of polyvinylpyrrolidone, 0.2-1.0%w/v of glucose, 15-40%w/v of compound amino acid injection, 5-30%w/v of propylene glycol, 10-40%w/v of PBS (phosphate buffer saline) vitamin E, 0.1-1.5%w/v of vitamin C and 0.1-1.0%w/v of aqueous solvent for injection. Due to the fact of containing no DMSO (dimethylsulfoxide) or protein, the tissue preserving fluid can avoid toxicity produced due to DMSO reverse infusion as well as safety worries of serum and proteins on clinical application, thereby being high in safety.

The invention discloses a method for measuring content of degraded impurity methionine sulfoxide in compound amino acid injection; an octadecyl silane bonded silica gel column is used, program setting is made by using Agilent sampler and derivatization is carried out, wavelength of 334 nm is detected after sampling, and the content is calculated according to an external standard method; for mobile phases, 0.038 mol/L of monopotassium phosphate (pH 6.9) and acetonitrile (88:12) acts as mobile phase A, acetonitrile acts as mobile phase B, and gradient diluting is carried out; test article diluent is water, and sampling concentration is 5 Mug/ml; according to the method of the invention, other amino acids and auxiliaries in the formulation are friendly to detection, the method has a good degree of separation and high specificity, the difficulty in detecting methionine sulfoxide in the amino acid compound is overcome, the methionine sulfoxide in a product is effectively controlled, and product quality is guaranteed.

The invention provides a determination method for determining tryptophan-related substances in an amino acid injection, and relates to the technical field of medicines. The determination method is used for determining the content of the tryptophan-related substances in amino acid injection. The method adopts high-performance liquid chromatography, adopts chromatographic column using octadecylsilane bonded silica as the stationary phase, and adopts the following three mobile phases: the mobile phase A being 0.01 mol/L sodium dihydrogen phosphate and having a pH value being adjusted with sodiumhydroxide solution and being 7.2; the mobile phase B being obtained through mixing acetonitrile, methanol and water according to a volume ratio of 45:45:10; and the mobile phase C being obtained through mixing methanol and water according to a volume ratio of 10:90 to carry out gradient elution. The relative retention time of tryptophan is analyzed and calculated to separate and determine the tryptophan-related substances in the amino acid injection. The determination method has the advantages of rapid analysis speed of the tryptophan-related substances, high accuracy and low inspection cost.

The invention belongs to the technical filed of a drug detection method and particularly relates to a rapid detection method of methionine sulfoxide in amino acid injection. An automatic amino acid analyzer is adopted for detection; only two mobile phases are adopted for separation, R1-R3 are derivative reaction reagents, Hitachi special ion exchange resin is taken as a filling agent, and the calculation is performed by peak area according to an external standard method. Through contrastive study, as a primary standard procedure adopts six mobile phases, the consumption of the reagents is high, and the mobile phases are various. As the two mobile phases are adopted, the consumption of the reagents is low, and a spectrogram is briefer. The test of the primary standard procedure needs 53.8 minutes, and the detection method provided by the invention only needs 29.5 minutes, so that the detection time is greatly reduced, and the efficiency is higher.

The invention relates to a medicinal composition of glutamine dipeptide sterile powder for injection and a compound amino acid injection, prepared with a solvent crystallization method, in particular to a combined application package. The medicinal composition comprises glutamine dipeptide sterile powder for injection, water for injection and a compound amino acid injection. Due to the adoption of the combined application package disclosed by the invention, great convenience is brought to the requirement of clinical administration; great convenience is brought to purchase and dispensation of an administration unit, and cost is saved; the combined application package is made for emergent use, and plays a great role in ensuring the stability of every single medicament, and the clinical application quality and bioavailability of a medicament can be enhanced; and the composition disclosed by the invention has the effect of remarkably reducing liver damage.