Culture method, application and combined preparation of hypoxia mesenchymal stem cell
A technology of mesenchymal stem cells and culture methods, applied in the field of application and combined preparations, and culture methods of hypoxic mesenchymal stem cells, to achieve the effects of high vitality and implantation survival rate, and prevention of functional decline of the body
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[0022] The culture method of hypoxic mesenchymal stem cells, under the hypoxic condition of 3-10%, preferably 5% oxygen concentration, culture according to the following steps: a, aseptic operation, cut umbilical cord or placenta into 0.5mm 3 ~1.5mm 3 Small tissue pieces (preferably 1mm 3 small tissue blocks), with Hank , After rinsing with s solution, remove the residual blood on the tissue block and separate the blood vessels; b. Digest the treated tissue block with trypsin, and stop the digestion when most of the cells are observed to disperse; c. Filter the digested material; d. Gained filtrate is centrifuged at low speed; d', use Hank , s solution centrifuges and washes the mesenchymal stem cell suspension 5 times; e, adding cell culture medium to the centrifuged filtrate to prepare a cell suspension. The cell culture medium can be Mesencult, a special medium for mesenchymal stem cells from StemCell Company of the United States. TM The culture reagent made can also be...
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