Culture method, application and combined preparation of hypoxia mesenchymal stem cell

A technology of mesenchymal stem cells and culture methods, applied in the field of application and combined preparations, and culture methods of hypoxic mesenchymal stem cells, to achieve the effects of high vitality and implantation survival rate, and prevention of functional decline of the body

Inactive Publication Date: 2010-08-04
晏泽
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  • Abstract
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Problems solved by technology

So far, no one has found that mesenchymal stem cells derived fr...

Method used

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Embodiment Construction

[0022] The culture method of hypoxic mesenchymal stem cells, under the hypoxic condition of 3-10%, preferably 5% oxygen concentration, culture according to the following steps: a, aseptic operation, cut umbilical cord or placenta into 0.5mm 3 ~1.5mm 3 Small tissue pieces (preferably 1mm 3 small tissue blocks), with Hank ， After rinsing with s solution, remove the residual blood on the tissue block and separate the blood vessels; b. Digest the treated tissue block with trypsin, and stop the digestion when most of the cells are observed to disperse; c. Filter the digested material; d. Gained filtrate is centrifuged at low speed; d', use Hank ， s solution centrifuges and washes the mesenchymal stem cell suspension 5 times; e, adding cell culture medium to the centrifuged filtrate to prepare a cell suspension. The cell culture medium can be Mesencult, a special medium for mesenchymal stem cells from StemCell Company of the United States. TM The culture reagent made can also be...

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Abstract

The invention discloses a culture method, application and a combined preparation of a hypoxia mesenchymal stem cell. By adopting an umbilical or placenta mesenchymal stem cell and combining the means of transfusing compound amino acid injection and the like, the vitality, the implantation survival rate and the treating effect of the mesenchymal stem cell in vivo can be improved; and in order to avoid the possibility of conglutination, conglomerating and cell mass embolism of the mesenchymal stem cell in blood vessel, the matching of saline containing heparin during treatment can be carried out by a mode of intravenous injection or intravenous drip. With more than three times of treatment, physical conditions of curee can be remarkably improved and objective indicators such as immunity, blood fat, blood sugar and the like can be remarkably improved.

Description

[0001] technical field [0002] The invention relates to the technical field of stem cell application, in particular to a culture method, application and combined preparation of hypoxic mesenchymal stem cells. [0003] Background technique [0004] Mesenchymal stem cells are an important member of the stem cell family, derived from the mesoderm and ectoderm in the early stages of development. Mesenchymal stem cells are a kind of adult stem cells with self-replication ability and multi-lineage differentiation potential. They belong to non-terminal differentiated cells. Under specific induction conditions in vitro, mesenchymal stem cells can differentiate into fibroblasts, fat cells, vascular endothelial cells, chondrocytes, bone cells, muscle cells, nerve cells, liver cells, cardiomyocytes, pancreatic beta cells and endothelial cells After continuous subculture and cryopreservation, various tissue cells still have multi-directional differentiation potential. Mesenchymal st...

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Application Information

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IPC IPC(8): C12N5/0775A61K35/50A61K35/44A61P43/00A61K31/198A61K31/401A61K31/405A61K31/4172A61K35/28
Inventor 晏泽
Owner 晏泽
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