The application relates to a method for detecting nano black carbon particles in animal biological samples, and relates to the field of atmospheric
particulate pollution research. The method is applied to black
carbon particle detection of human samples. After the animal biological sample is freeze-dried, concentrated
nitric acid is used for
digestion, the pH value is adjusted, diatomite is added, mixed and settled, high-speed
filtration is carried out, nano black carbon particles are captured on a
quartz fiber filter membrane pre-paved with diatomite, and the filter membrane is washed with an oxidant and an
organic solvent to remove residual
organic matter. The filter membrane is moved into a reaction kettle, concentrated
nitric acid is used for oxidation under high temperature and
high pressure, black carbon particles are oxidized into corresponding molecular markers
benzene polycarboxylic acid, integrated separation, enrichment, purification and derivative treatment are carried out, and GC-MS / MS is used for quantitative analysis of the
benzene polycarboxylic acid. The concentration of the
benzene polycarboxylic acid is obtained after conversion, and the concentration of black carbon in the biological sample is obtained. The benzene polycarboxylic acid
molecular marker method is successfully applied to the detection of ultra-trace nano black carbon particles in animal biological samples, and accurate quantitative analysis of black carbon particles in biological samples is realized.