Clinical mesenchymal stem cell cryopreservation solution and preparation method thereof

Abstract

The invention discloses clinical mesenchymal stem cell cryopreservation solution and a preparation method thereof. The preservation solution comprises the following components in percentage by weight:1-5% of autologous plasma lysate mixed liquor, 1-2% of human serum albumin, 0-1% of VC injection, 5-10% of glucose injection, 20-30% of electrolyte-like solution, 20-30% of glucosamine injection, 20-30% of compound amino acid injection, 2-5% of dimethyl sulfoxide and 2.5-5% of trehalose. The prepared cryopreservation solution can be used for deep low-temperature and long-term storage of cells, and can realize long-distance transportation, so that the distribution range of cells is expanded, the long-distance treatment of stem cells is realized, and the cells can be directly returned after recovery so as to reduce the potential safety hazards caused by complicated operations.

Description

technical field

[0001] The invention belongs to the technical field of cell preservation, and in particular relates to a cryopreservation solution for clinical use of mesenchymal stem cells and a preparation method thereof. Background technique

[0002] Cell therapy involves the process of cell preparation, expansion, reinfusion, etc. Usually, the place and time of these processes are not consistent, so the cells need to be transported to the destination, and the quality control in the process of cell transport is weak. In the normal temperature environment of the cell, various enzymes in the cell react with other biological macromolecules, the metabolism is vigorous, the aerobic is high, and the energy consumption is large. These physiological processes will produce a large number of oxygen free radicals and lipid peroxides. If they are not removed in time, they will cause changes in environmental pH and osmotic pressure, resulting in cell swelling and death. Due to the inc...

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