The invention discloses a preparation process of wall broken spirulina protein powder. The preparation process of the wall broken spirulina protein powder comprises the following steps of: pulverizing wall broken spirulina with a pulverizer, soaking wall broken spirulina powder in water for 5-10 hours, taking out the soaked wall broken spirulina powder, and pulverizing under ultrasonic waves; soaking spirulina cells with distilled water or a low-salt solution directly so that the spirulina cells swell and break to release wall broken spirulina protein, wherein the wall broken spirulina protein is soaked in distilled water for 10 days, or the wall broken spirulina protein is soaked in the low-salt solution for 3-4 days; weighing 5.99g of wall broken spirulina protein powder, and soaking in distilled water for 12 days for swelling fully; filtering with a piece of filtering cloth of 74mu m, centrifuging a filtrate at the temperature of 4 DEG C and 5500g for 20 minutes, removing spirulina residues, and taking a purplish red supernatant which is the crude extract of wall broken spirulina protein. The preparation process of the wall broken spirulina protein powder is simple, and the prepared wall broken spirulina protein powder has a high nutritional value.