Terahertz wave technology-based unmarked aquaporin function assessment method

Abstract

The invention relates to a terahertz wave technology-based unmarked aquaporin function assessment method. According to the method, cells are inoculated on the surface of a constructed terahertz metamaterial chip, culture is carried out to ensure that the cells grow in an adherent state and are fused into a single-cell layer, and a terahertz time-domain spectrograph is adopted to measure the harmonic peak condition of a cell layer in isotonic buffer solution; and hypotonic buffer solution is changed, and a large number of water molecules enter the cells through aquaporins to ensure that the volumes of the initial cells are increased so as to form swelled cells. According to the method, the terahertz time-domain spectrograph is adopted to obtain the harmonic peak change condition of the adherent cell layer during osmotic pressure change, so that the cell swelling degree is effectively reflected, namely, the osmosis condition of the water molecules passing through the cell layer; and thereciprocal of a time required for achieving a maximum response value is solved according to a real-time response curve, so that the unmarked assessment of the cell aquaporin function condition can berealized. The method can be used for high-throughput screening of correlated targeted AQP drugs.

Description

Technical field [0001] The invention belongs to the technical field of terahertz measurement, and relates to a method for label-free evaluation of aquaporin functions based on terahertz wave technology. Background technique [0002] Water is the source of life. Aquaporin (AQP) is a transmembrane protein located on the cell membrane, a channel for selective and efficient transport of water molecules. Since the first report of the first aquaporin (AQP1) in 1991, researchers have successively discovered that 13 aquaporins widely distributed in different organs play a vital role in maintaining the normal physiological functions of the organs. The expression and functional status of AQP are closely related to tumors, cerebrovascular diseases, kidney diseases and other diseases. In particular, AQP is up-regulated in tumor tissues, and mediates their migration, proliferation and invasion by rapidly transporting water molecules into tumor cells, and participates in the formation of tum...

AI Technical Summary

Problems solved by technology

However, the application of THz waves to live cell detection still faces the problem of scale mismatch between the THz wavelength (1THz-300μm) and the thickness of the cell layer (about 5-10μm), and the strong absorption interference of water in the liquid phase environment (water at 1THz The absorption coefficient is about 230cm -1 )And other issues

At present, there are no relevant reports on the application of terahertz wave technology in the field of label-free assessment of cellular AQP function

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