The disclosure relates to a method for eliminating interference of
theophylline in
immunoassay and an immunodetection kit. The method for eliminating interference of
theophylline in
immunoassay includes: performing an
immunoassay in the presence of a
theophylline interference-resistant agent, the theophylline interference-resistant agent having a final concentration of 0.1 mg / mL-100 mg / mL, and being selected as at least one component from a group consisting of free 6-
mercaptopurine, 6-thioguanine, 8-azguanine,
hypoxanthine, adenine,
purine, 2,6,8-trioxypurine,
adenine nucleotide, and
adenosine. The method and the kit can effectively reduce interference of theophylline on immunoassay results.