10 results about "Adenine nucleotide" patented technology

Provided are antibodies capable of binding an antigen in an adenine nucleotide-dependent manner. Examples of adenine nucleotides include ATP, ADP and AMP, which are frequently present in tumor microenvironment at much higher concentrations in normal tissues. Such antibodies, if designed to target a tumor associated antigen or immune checkpoint molecule, can target tumor tissues more specifically than conventionally antibodies and thus have improved therapeutic index.

Provided are interfering RNAs such as small interfering RNAs (siRNAs) targeting the messengerRNA of adenine nucleotide translocator 2 (ANT2), pharmaceutical compositions comprising such, and therapeutic uses thereof for treating diseases associated with ANT2, for example,cancer and metabolic diseases.

The invention discloses a strain capable of efficiently producing 3-hydroxy-2-picolinic acid, a construction method and application, and relates to the technical field of gene engineering. According to the 3-hydroxy-2-picolinic acid heterologous expression strain and the application thereof, homologous L-lysine-2-aminotransferase PyrB, bi-component flavin-dependent monooxygenase PyrC / PyrK and flavin adenine nucleotide-dependent dehydrogenase PyrD are cloned from streptomyces picoliniamycin NRRL B-2517 by virtue of a genetic engineering technology, the homologous L-lysine-2-aminotransferase PyrB, the bi-component flavin-dependent monooxygenase PyrC / PyrK and the flavin adenine nucleotide-dependent dehydrogenase PyrD are combined with a strong promoter, and an RBS sequence is optimized, so that the 3-hydroxy-2-picolinic acid heterologous expression strain is successfully constructed; a core foundation is laid for efficient, green and low-cost microbial fermentation production of the product. According to the microbial fermentation method, self production of 3-hydroxy-2-picolinic acid by cells is achieved through metabolic pathway reconstruction, the whole reaction process is a water-phase reaction and can be carried out at normal temperature and normal pressure, toxic reagents and additional coenzymes do not need to be used, the product can be secreted out of the cells, the process is simple and convenient, and the method is a route with great large-scale production potential.

The invention relates to a novel enzyme composition comprising at least two different thermostable polypeptides having type II DNA methyltransferase activity as well as a restriction / modification system in particular for the transformation of microorganisms of the genus Caldicellulosiruptor, wherein said polypeptides methylate an adenine in a asymmetric DNA recognition site, where the adenine nucleotide is followed by a thymine nucleotide in the linear sequence of bases along its 5′→3′ direction, wherein the DNA recognition site is 5′-GCATC-3′ and / or wherein said polypeptide methylate the adenine in a complement DNA recognition site, where a thymine nucleotide is followed by a adenine nucleotide in the linear sequence of bases along its 3′→5′ direction, wherein the DNA recognition site is 3′-CGTAG-5′.

The disclosure relates to a method for eliminating interference of theophylline in immunoassay and an immunodetection kit. The method for eliminating interference of theophylline in immunoassay includes: performing an immunoassay in the presence of a theophylline interference-resistant agent, the theophylline interference-resistant agent having a final concentration of 0.1 mg / mL-100 mg / mL, and being selected as at least one component from a group consisting of free 6-mercaptopurine, 6-thioguanine, 8-azguanine, hypoxanthine, adenine, purine, 2,6,8-trioxypurine, adenine nucleotide, and adenosine. The method and the kit can effectively reduce interference of theophylline on immunoassay results.

This disclosure relates to the field of poly-adenylated (poly-A) tails. In some embodiments, a DNA encodes a poly-A tail located 3′ to nucleotides encoding a protein of interest, wherein the poly-A tail comprises one or more non-adenine nucleotide.

This disclosure relates to the field of poly-adenylated (poly-A) tails. In some embodiments, a DNA encodes a poly-A tail located 3′ to nucleotides encoding a protein of interest, wherein the poly-A tail comprises one or more non-adenine nucleotide.

The present invention addresses the problem of providing a novel method for suppressing the amount of a by-product derived from an unreacted oligo dT primer remaining after a reverse transcription reaction in cDNA amplification based on a Poly-A tagging method.　Provided is a method for adding a poly-A sequence onto the 3'-end side of a DNA strand, the method comprising: a reverse transcription step for obtaining a DNA strand extended to the 3'-end side by using, as a template, an RNA strand having a poly-A sequence on the 3'-end side thereof, and performing reverse transcription using a first oligo dT primer; and an addition step for adding a poly-A sequence onto the 3'-end side of the DNA strand using a terminal deoxynucleotidyl transferase or an active fragment thereof under conditions in which the intramolecular base pairing between a thymine base and an adenine base added to the 3'-end side of the first oligo dT primer that has not been extended to the 3'-end side in the reverse transcription step is stabilized, wherein the addition step involves adding the poly-A sequence in the presence of an ammonium cation and / or a modified adenine nucleotide, and / or at a temperature of at most 34ºC, and / or the first oligo dT primer used in the reverse transcription step includes a modified thymine nucleoside.

The application discloses a kind of low-defect few-layer graphene water-based conductive paste and its preparation method and application, belong to conductive paste technical field;Low-defect few-layer graphene water-based conductive paste of the application, by weight fraction, including 1~3 mass fraction low-defect few-layer graphene, 100 mass fraction ultrapure water, 0.8~1.2 mass fraction conductive additive;The low-defect few-layer graphene is first to graphite powder at room temperature intercalation, then chemical expansion is carried out, and then water phase stripping is obtained using 5 '-adenine nucleotide salt aqueous solution;The conductive additive is amphiphilic polythiophene;The amphiphilic polythiophene is treated to obtain using hydrogen bromide and anhydrous acetic anhydride after polymerization with 2,5-dibromo-3-triethylene glycol monomethyl ether thiophene, 3-[10-(4-methylphenyl ether) decyl)] thiophene as monomer;The low-defect few-layer graphene water-based conductive paste prepared by the application has less graphene defects, good dispersibility and good conductivity.