82 results about "Adenine nucleotide" patented technology

A therapeutic and / or cosmetic formulation comprising at least one anti-sense polynucleotide to a connexin protein together with a pharmaceutically acceptable carrier or vehicle is useful in site specific down regulation of connexin protein expression, particularly in reduction of neuronal cells death, wound healing, reduction of inflammation, decrease of scar formation and skin rejuvenation and thickening.

A composition comprising a novel Ca2+-activated, [ATP]i-sensitive nonspecific cation (NCCa-ATP) channel is described. The channel is found in mammalian neural cells and exhibits a different sensitivity to block by various adenine nucleotides, and is activated by submicromolar [Ca]i. The NCCa-ATP channel is activated under conditions of ATP depletion, which causes severe cell depolarization, followed by cell swelling. The NCCa-ATP channel is regulated by a sulfonylurea receptor and is inhibited by sulfonylurea compounds glibenclamide and tolbutamide. Methods employing compositions comprising the NCCa-ATP channel to screen for compounds that block the channel and the use of such antagonists as therapeutics in preventing brain swelling and damage are described. In addition, methods employing compositions comprising the Kir2.3 channel to screen for compounds that open the channel and the use of such antagonists as therapeutics in preventing brain swelling and damage are described.

The present invention relates to a method for quickly determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides without the use of radioactive agents.Particularly, the present invention provides a method of determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides selected from the group consisting of cAMP produced by endogenous adenylate cyclase, and AMP, ATP, ADP and a mixture thereof, which comprises (1) combining a biological sample with effective amounts of apyrase, adenosine deaminase and alkaline phosphatase to enzymatically remove non-cyclic adenine nucleotides other than cAMP, and glucose-6-phosphate in the sample; (2) enzymatically converting cAMP into AMP; (3) determining an amount of AMP without the use of radioactive agents, and a kit to carry out the method.

The invention relates to the technical field of detection sensors and particularly relates to an electrochemical aptamer electrode for kanamycin detection. Graphene-polyaniline compound, polyamide-amine dendritic high molecule-gold nano compound, kanamycin anti-body are modified on a glass carbon electrode in sequence from bottom to up; the surface of the electrode is sealed with BSA (Bovine Serum Albumin) and single chain DNA (Deoxyribose Nucleic Acid) containing 10 adenine nucleotide. The preparation method is simple, stable in performance and good in electrode repeating performance and is suitable for practical applications of kanamycin detection in food safety and bio-sensor industrialization; the prepared electrode is low in technical cost and is suitable for the requirement of low cost in industrialization; according to a sandwich type electrochemical sensing system fixed by using the glass carbon electrode as a fixing carrier and based on a DNA aptamer, rapid and online kanamycin detection in food is realized and the detection limit is 4.6x10<-6>mu gmL<-1>.

The invention discloses a microRNA-328 and the application of antisense nucleotide thereof for diagnosing and controlling heart diseases; in the invention, a plurality of experiments prove that the microRNA-328 is changed and has pathopoiesia function in heavy heart diseases (atrial fibrillation, ischemia-reperfusion or myocardial fibrosis), and the antisense nucleotide AMO-328 of the microRNA-328has prevention and cure functions to the heart diseases, the microRNA-328 is used as a novel drug effect target spot, so as to finally determine and detect that the peripheral blood microRNA-328 canbe used as a novel biological marker which is used for diagnosing and preventing the heart diseases; in addition, the peripheral blood microRNA-328 expression level of a patient can be detected through fluorescent quantitative PCR and the nanometer microballoon technology enhanced by silver staining, so as to be used for diagnosing and preventing the heart diseases such as atrial fibrillation, long QT syndrome, ischemia-reperfusion or myocardial fibrosis and the like.

The invention relates to a nanometer gold complex, as well as a preparation method and an application of the nanometer gold complex. Specifically, the nanometer gold complex provided by the invention comprises nanometer gold particles and a functional molecule, wherein the functional molecule is bound to the surfaces of the nanometer gold particles through an oligonucleotide binding region which is rich in adenine nucleotide or the analogue of the adenine nucleotide, and the functional molecule comprises the oligonucleotide binding region which is rich in the adenine nucleotide or the analogue of the adenine nucleotide, a functional region and a transition area located between the binding region and the functional region. The invention also provides the preparation method and the application of the nanometer gold complex.

The invention discloses a feed for treating mucormycosis of cyclemys trifasciata and a preparation method of the feed, and belongs to the technical field of testudinate feed processing. The feed comprises the following raw materials: earthworm powder, pomacea canaliculata powder, pumpkin powder, maize meal, salt, dimethyl-beta-propiothetin, glycine betaine, threonine, adenine nucleotide, sodium diacetate, sodium guanylate and traditional Chinese medicine preparation. The feed is prepared by the steps of stoving, pulverizing, screening, mixing, pelletizing, stoving, sterilizing and the like. The feed synthesizes efficacies of various traditional Chinese medicines, plays the functions of resisting bacterium, diminishing inflammation, enhancing the immunologic function of the body, and the like, and can effectively improve the cure rate of the mucormycosis of the cyclemys trifasciata and reduce the death rate. The feed is reasonable in preparation and rich in nutrition, so that the feed intake of the cyclemys trifasciata with the mucormycosis can be increased, and the growth rate is improved; the feed is sterilized at high temperature and high pressure, the time is short, the effect is good, and the retention time is long.

The invention discloses a catalytic light mark and a preparation method thereof, and a method for determination of trace uranium by the catalytic light mark. The catalytic light mark is composed of two parts of a DNA enzyme and a substrate DNA, a 3' end of a DNA enzyme sequence comprises four continuous guanine deoxynucleotides (G), the middle of a substrate DNA sequence has an adenine nucleotide (rA), and a 5' end of the substrate DNA is modified by a 4-methyl-6-carboxyl rhodamine (TAMRA) fluorescent dye. The DNA enzyme and the substrate DNA are in mixing hybridization to form the catalytic light mark, the catalytic light mark is added to a reaction system of a to-be-measured sample to undergo a reaction, the fluorescence intensity of uranyl nitrate ions with known concentration and the fluorescence intensity of the sample are determined, and the uranium content in the sample can be obtained through calculation. The method is a fast, accurate, high-sensitivity and high-selectivity method for determination of trace uranium.

The invention relates to a method for detecting the polymorphism of single nucleotide with gold nanoparticles in the nanotechnology field. The method comprises the following steps of: constructing an unsymmetrical two-dimensional pattern by utilizing a DNA (Deoxyribonucleic Acid) origami technology to prepare a DNA origami chip; preparing a colloidal gold probe; hybridizing a DNA short chain and the colloidal gold probe; connecting the particle-size colloidal gold probe and the DNA origami chip in the unsymmetrical two-dimensional pattern; and detecting the polymorphism of the single nucleotide. The invention has the advantages of simpleness, low cost, and the like without strict experimental conditions and complicated enzyme reactions and can realize a DNA hybridization process in a nano-scale liquid-phase reaction and achieve high-sensitivity biomedical detection.

The invention provides a method for constructing an artificial multi-enzyme system based on a lanthanide series nucleotide compound and a DNA (Deoxyribonucleic Acid) oriented immobilization technologyand belongs to the field of preparation of immobilized enzymes. The method comprises the following steps: firstly, preparing single-stranded DNA modified magnetic nanoparticles and complementary single-stranded DNA functionalized glucose oxidase; then mixing the magnetic nanoparticles with the enzyme; carrying out DNA complementary hybridization to realize the immobilization of the enzyme; commonly hatching the immobilized enzyme, cerium nitrate hexahydrate and disodium adenosine 5'-phosphate; carrying out self-assembling on a lanthanide series nucleotide coordinated polymer to realize the encapsulation of the immobilized enzyme and constructing the artificial multi-enzyme system. An encapsulation vector can realize the effect of protecting the immobilized enzyme and is also used as an analogue enzyme to form an enzyme cascade system with the immobilized natural enzyme. A preparation process of the artificial multi-enzyme system, provided by the invention, is simple, has moderate conditions and achieves high enzyme activity; the artificial multi-enzyme system is easy to separate from a reaction system and has excellent stability and reusability; meanwhile, the analogue enzyme is introduced and the cost is extremely reduced.

The invention discloses a feed for treating a rotten-skin disease of cyclemys trifasciata and a preparation method of the feed, and belongs to the technical field of testudinate feed processing. The feed comprises the following raw materials: pomacea canaliculata powder, earthworm powder, maize meal, sweet potato powder, salt, dimethyl-beta-propiothetin, glycine betaine, alanine, adenine nucleotide, sodium diacetate, sodium guanylate and traditional Chinese medicine preparation. The feed is prepared by the steps of stoving, pulverizing, screening, mixing, pelletizing, stoving, sterilizing and the like. The feed synthesizes efficacies of various traditional Chinese medicines, plays the functions of resisting bacterium, diminishing inflammation, enhancing the immunologic function of the body, and the like, can effectively improve the cure rate of the rotten-skin disease of the cyclemys trifasciata and reduce the death rate, reduces the cultivation risk, and improves the economic benefits. The feed is reasonable in preparation and rich in nutrition, so that the feed intake of the cyclemys trifasciata with the rotten-skin disease can be increased, the growth rate is improved, and the growth cycle is shortened; the feed is sterilized at high temperature and high pressure, the time is short, the effect is good, and the retention time is long.

The present invention discloses a feed for treating Cyclemys trifasciata bacterial enterorrhagia, and a preparation method thereof, and belongs to the technical field of chelonia feed processing. The feed comprises the following raw materials: earthworm powder, Pomacea canaliculata powder, corn powder, sweet potato powder, edible salt, dimethyl-beta-propiothetin, betaine, phenylalanine, guanylic acid-adenylic acid, sodium diacetate, guanylic acid sodium, and a traditional Chinese medicine preparation, and drying, crushing, screening, mixing, granulating, drying, sterilization and other steps are performed to prepare the feed. According to the present invention, a variety of traditional Chinese medicine effects are integrated, effects of anti-bacterial effect, anti-inflammatory effect, body immune function enhancing and the like can be provided, the cure rate of the Cyclemys trifasciata bacterial enterorrhagia can be effectively improved, and the death rate can be reduced; the feed of the present invention has characteristics of reasonable preparation, rich nutrition, increase of the intake of the Cyclemys trifasciata having bacterial enterorrhagia, and growth rate improving; and the feed preparation method of the present invention has characteristics of simple operation and low production cost, and is easy to promote and apply.

The invention discloses application of adenine nucleotide translocator 1 (ANT1), and relates to the field of the treatment of cardiovascular diseases. Through down-regulating the level of the sulfydryl nitrosylation modification of the ANT1 in a myocardial cell, the occurrence of myocardial hypertrophy is effectively inhibited. A method comprises the following steps of transfecting a myocardial cell by utilizing an adenovirus with the modification site mutation of cysteine, and regulating and controlling the expression of the sulfydryl nitrosylation modification of ANT1 protein in a cell, andalso comprises the following steps of specifically transfecting the heat tissue of a mouse by utilizing an adeno-associated virus with the modification site mutation of the cysteine, and regulating and controlling the expression of the sulfydryl nitrosylation modification of ANT1 protein in the heart tissue. Therefore, the sulfydryl nitrosylation modification of the ANT1 protein can be used as a new important target for clinically treating the myocardial hypertrophy, and has potential clinical application value in the prevention and treatment of the myocardial hypertrophy.

Provided are diagnostic methods and kits including oligo and / or polynucleotides or derivatives, including as well antibodies determining whether a human subject is at risk of getting adverse drug reaction after statin therapy or whether the human subject is a high or low responder or a good a or bad metabolizer of statins. The diagnostic methods and kits including antibodies determining whether a human subject is at risk for a cardiovascular disease. Also provided are polymorphic sequences and other genes and isolated polynucleotides encoding a phenotype associated (PA) gene polypeptide useful in methods to identify therapeutic agents and useful for preparation of a medicament to treat cardiovascular disease or influence drug response.

The invention discloses a meat tenderizing method for improving the pyrolysis of myofibril and belongs to the field of food processing technologies. The meat tenderizing method is characterized in that fresh or unfrozen raw meat is cleaned and put into clear water with the mass of 0.8-1.2 times that of the raw meat, and a meat tenderized agent with the mass 4-8% of that of the raw meat is added, stirred uniformly, soaked and drained off to obtain tenderizing raw meat; adenine nucleotide, salt and sodium lactate constitute the meat tenderized agent. According to the invention, the salt and sodium lactate are used for restraining adenine nucleotide deaminizing enzyme; compared with the conventional salt pickling method, more than 40% of a shearing force can be lowered, the cooking loss is reduced, the color and luster are improved, the freshness and nutritive value of meat can be improved, and the quality of the meat can be improved, so that the meat tenderizing method has a wide application prospect in the meat processing field.

Adenine nucleotides such as adenosine 5′-monophosphate (AMP), adenosine 5′-diphosphate (ADP) or adenosine 5′-triphosphate (ATP) and / or adenosine and inorganic phosphate are administered in the treatment of advanced cancer in an out-patient setting and without serious side effects. Administration of up to 100 micrograms / kilogram / minute of continuous intravenous infusions of ATP for 8-10 hours, can be performed on an out-patient basis to patients who do not otherwise require hospitalization. No serious side effects, defined as grades 3 or 4 toxicity are observed during such an infusion.

The present invention discloses a feed for treating small water turtle saprolegniasis and a preparation method thereof, and belongs to the technical field of terrapin feed processing. The feed includes the following raw materials: pomaceacanaliculata powder, locust powder, pumpkin powder, corn flour, salt, dimethyl-beta-propiothetin, betaine, phenylalanine, adenine nucleotides, sodium diacetate, sodium guanylate and Chinese medicine preparations. The feed is prepared by drying, grinding, sieving, mixing, granulating, drying, sterilization and other steps. The present invention combines the effectiveness of a variety of traditional Chinese medicine, and has effects of resisting to bacteria, diminishing inflammation, clearing away heat and toxic materials, improving immune function and the like. The small water turtles have good absorption efficiency of the feed, the small water turtle mortality rate caused by the saprolegniasis can be effectively reduced, and farming risks are reduced. The feed has reasonable formulation and rich nutrition, can increase intake of the small water turtles with saprolegniasis, can improve growth rate, and can shorten growth period. The preparation method has simple operation and low production costs, and is conducive to application promotion.

Described herein are assays, kits and methods for treating mood disorders by testing for one or more polymorphisms in a specific group of genes and for analyzing the results of polymorphism testing; the genes included may converge in one or more signaling pathways, and may be epigenetic. The genes are included based on the relationships of the proteins encoded by the genes in the context of particular signaling pathways and provide a diagnostically relevant nexus. Also described herein are methods of presenting the data collected by the screen, including methods of delivering interpretive comments and / or treatment guidance based on the results of the genetic screening either individually or based on the genetic composition of particular clusters of genes which may be related to each other. Importantly, drugs which modulate these genetic disturbances are described for targeted therapeutic use based upon companion diagnostic method.

The structure of a soluble, functional fragment of human Apaf-1 protein having ADP bound thereto is disclosed. The invention includes such soluble, functional fragments of human Apaf-1 and other metazoan Apaf-1 homologs. Also included in the invention are methods of making such fragments and methods of using them, for example in screening methods to identify adenine nucleotide analogs and other compounds useful for alleviating or preventing disease conditions associated with inappropriate regulation of apoptosis.

The present invention relates to a small interfering RNA (siRNA) suppressing the expression of adenine nucleotide trnaslocator 2 (ANT2) gene and an anticancer agent containing the same. Particularly, the invention relates to ANT2 siRNA comprising a sense sequence selected from the nucleotide sequences of ANT2 mRNA, a hairpin loop sequence and an antisense sequence binding complementarily to the said sense sequence and an anticancer agent containing the same. ANT2 siRNA of the present invention inhibits the expression of ANT2 gene, suggesting that it inhibits the growth of cancer cells exhibiting high level of ANT2. Therefore, ANT2 siRNA of the invention can be effectively used for gene therapy for cancer treatment and further prevents the anticancer effect from decreasing by anticancer drug resistance of cancer cells.

The invention relates to an adenosine monophosphate (AMP) adenosine deaminase for synthesizing and metabolizing an inosine monophosphate from adenine nucleotide of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis from 'Bailing' production strain, a gene coding the enzyme and application thereof. The AMP adenosine deaminase comprises the proteins shown in SEQ ID No. 1 and SEQ ID No. 2, and the coding gene of the AMP adenosine deaminase corresponds to the nucleotide sequence shown in SEQ ID No. 3 and SEQ ID No.4. According to the invention, the metabolizing way for synthesizing the inosine monophosphate from the adenine nucleotide is researched in detail from the principle, and the cloned DNA containing the nucleotide sequence provided by the invention can be transferred to an engineering strain by transduction, transformation, and combined transfer; the expression of the gene is biologically synthesized by regulating the inosine monophosphate, the host inosine monophosphate is endowed with high expressivity, an effective way is provided for increasing the yield of the inosine monophosphate, and the invention has a great application prospect.

The invention relates to an adenine phosphoribosyltransferase for synthesizing and metabolizing an adenine from adenine nucleotide of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis from 'Bailing' production strain, a gene coding the enzyme and application of the adenine phosphoribosyltransferase. The amino acid sequence of the adenine phosphoribosyltransferase is shown in SEQ ID No. 1, and the coding gene of the adenine phosphoribosyltransferase corresponds to the nucleotide sequence shown in SEQ ID No. 2. According to the invention, the metabolizing way for synthesizing the adenine from the adenine nucleotide is researched in detail from the principle, and the cloned DNA containing the nucleotide sequence provided by the invention can be transferred to an engineering strain by transduction, transformation and combined transfer; through regulating the gene expression of the adenine biological synthesis, the host adenine is endowed with high expressivity, an effective way is provided for increasing the yield of the adenine, and the invention has a great application prospect.

The present invention discloses a wattle-necked soft-shell turtle dysentery preventing and treating feed and a preparation method thereof, which belongs to the technical field of turtle feed processing. The feed includes the following raw materials: bone meal, earthworm powder, pumpkin powder, corn flour, edible salt, dimethyl-beta-propiothetin, betaine, threonine, adenine nucleotides, sodium diacetate, guanosine sodium and Chinese herbal medicine preparations. The feed is produced by drying, crushing, screening, mixing, granulating, drying, cooling, packaging and other steps. The wattle-necked soft-shell turtle dysentery preventing and treating feed of the present invention combines the effects of various Chinese herbal medicines, can play roles in preventing bacteria, protecting liver, clearing internal heat, clearing heat and purging intense heat, removing dampness and regulating middle warmer, etc., and enhances the disease resistance, effectively prevents the dysentery and improves the survival rate of the wattle-necked soft-shell turtles. The wattle-necked soft-shell turtle dysentery preventing and treating feed of the present invention has reasonable preparation and strong palatability, and increases the food intake, promotes the growth rate and shorten the growth cycle of the wattle-necked soft-shell turtles.

The present teaching provides a fluorescent oligonucleotide probe having a high degree of design flexibility and wide applicability, as well as the use thereof. This is an oligonucleotide probe capable of forming a stem and loop, comprising at least one fluorophore located between adjacent nucleotides in the stem and is linked to a unit represented by Formula (1) and at least one quencher located at a site capable of pairing up with the at least one fluorophore located between the adjacent nucleotides in the stem and is linked to a unit represented by Formula (2). (In the formulae, X represents the fluorophore, Y represents the quencher, R1 represents an optionally substituted C2 or C3 alkylene chain, R2 represents an optionally substituted C0-2 alkylene chain, and Z represents a direct bond or linker.)

The invention discloses a feed for treating a red neck disease of cyclemys trifasciata and a preparation method of the feed, and belongs to the technical field of testudinate feed processing. The feed comprises the following raw materials: earthworm powder, locust powder, pumpkin powder, sweet potato powder, salt, dimethyl-beta-propiothetin, glycine betaine, threonine, adenine nucleotide, sodium diacetate, sodium guanylate and traditional Chinese medicine preparation. The feed is prepared by the steps of stoving, pulverizing, screening, mixing, pelletizing, stoving, sterilizing and the like. The feed synthesizes efficacies of various traditional Chinese medicines, and plays the functions of resisting bacterium, diminishing inflammation, enhancing the immunologic function of the body, and the like. The feed absorbing effect of the cyclemys trifasciata is good, the duration time is long, the disease resistance of the cyclemys trifasciata can be improved, the death rate of the cyclemys trifasciata due to the red neck disease can be effectively reduced, and the cure rate of the red neck disease of the cyclemys trifasciata can be improved. The feed is reasonable in preparation and rich in palatability, so that the feed intake of the cyclemys trifasciata with the red neck disease can be increased, the growth rate is improved, and the growth cycle is shortened.

The invention discloses a method for preparing nanocluster gel of a hydroxyl-like phosphorite component. The method comprises the following steps: mixing a chloroauric acid solution and a adenine nucleotide series, reacting for 1 to 60 minutes, adding a citric acid buffering solution, standing for 0.5 to 24 hours in a light-shielding manner, adding absolute ethyl alcohol, uniformly mixing, and centrifuging to obtain precipitates, preparing the obtained precipitates into an adenine nucleotide shell nanocluster with the concentration of 0.1 to 10000 microgram / mL, adding metal ions to prepare sediments, dispersing the sediments in deionized water, thus obtaining the nanocluster gel. The fluorescent gold nanocluster adopting the adenine nucleotide series as ligand reacts with the metal ions toobtain aggregation-induced fluorescent nanocluster gel, the aggregation of a metal-phosphate structure achieves the fluorescent reinforced effect, the combination force of gold and adenine in the nanocluster gel is slightly weak, can be dissociated under the existence of molecules such as glutathione in the cell, the degradability is excellent, and the preparation method is simple in operation, high in yield, and low in cost.

A composition comprising a novel Ca2+-activated, [ATP]i-sensitive nonspecific cation (NCCa-ATP) channel is described. The channel is found in mammalian neural cells and exhibits a different sensitivity to block by various adenine nucleotides, and is activated by submicromolar [Ca]i. The NCCa-ATP channel is activated under conditions of ATP depletion, which causes severe cell depolarization, followed by cell swelling. The NCCa-ATP channel is regulated by a sulfonylurea receptor and is inhibited by sulfonylurea compounds glibenclamide and tolbutamide. Methods employing compositions comprising the NCCa-ATP channel to screen for compounds that block the channel and the use of such antagonists as therapeutics in preventing brain swelling and damage are described. In addition, methods employing compositions comprising the Kir2.3 channel to screen for compounds that open the channel and the use of such antagonists as therapeutics in preventing brain swelling and damage are described.

A method of search for and identification of a eukaryotic IRES element active in cap-independent translation of RNA in eukaryotic cells is provided, comprising the following steps: i) screening eukaryotic mRNA sequences or corresponding DNA sequences for a potential IRES element having a block of nucleotides having: a) a length of at least 30 nucleotides; b) an adenine nucleotide content of at least 40 mol-%; and c) a pyrimidine nucleotide content of less than 40 mol-%; ii) inserting said potential IRES element into a linear dicistronic construct between an upstream gene and a downstream GUS reporter gene, whereby said potential IRES element is positioned for IRES-dependent translation of said downstream GUS gene and whereby said upstream gene is preceded by a stable hairpin structure to prevent IRES-independent translation of said genes; and iii) testing said potential IRES element for IRES-dependent translation of said GUS gene in a rabbit reticulocyte lysate or in a wheat germ extract in vitro translation assay, whereby GUS gene expression is quantitated preferably relative to a construct having a reference IRES element or a non-IRES element between said upstream gene and said GUS gene.