1 results about "Adenine nucleotide" patented technology

The application belongs to the field of biological detection, and particularly relates to a method for detecting nucleosome histone and its modifier based on single molecule immunological technology. Based on single molecule technology, the nucleosome sample to be detected which is tailed by polyadenylic acid (Poly-A) is specifically captured by antibody modified magnetic beads, then hybridized with biotin labeled polythymidylic acid (Poly-T) to form an immunocomplex, and then recognized by streptavidin labeled beta-galactosidase (SbetaG) in a single molecule array chip, so that the visualization, numerical quantification and detection of the sample circulating nucleosome and its modifier are realized. The method has high sensitivity, repeatability and wide dynamic detection range, and can realize the accurate quantification of histone H3 and its six key epigenetic modifiers in circulating nucleosome by using only 20 muL of serum sample, which is beneficial to reveal the variation of circulating nucleosome histone modifier in the early development stage of various diseases, and has good clinical application prospect.