The invention discloses a cucumber CsEIN2
gene and an application of the cucumber CsEIN2
gene in regulating and controlling the
cold resistance of cucumbers. The cucumber CsEIN2
gene is identified for the first time, a CDS sequence of the cucumber CsEIN2 gene is cloned from a cucumber full-length
cDNA library, a gene editing carrier pCas9-CsEIN2 targeting the CsEIN2 gene is constructed on the basis of the CDS sequence, the
plasmid is transformed into the cucumber to obtain a CsEIN2 gene modified
plant, and through field cross
pollination,
molecular identification and
phenotype identification, the CsEIN2 gene modified
plant is obtained. It is proved that the CsEIN2 gene has the function of regulating and controlling cucumber
cold stress resistance, the regulation and control are positive regulation and control, and when the function of the CsEIN2 gene is lost or weakened, the
cold damage index of the cucumber is remarkably increased, and the
cold resistance is reduced. The invention lays a foundation for revealing genetic and biological mechanisms of cucumber
cold resistance, provides a new gene resource for cultivation of cold-resistant cucumbers, and has important significance for research on cucumber cold resistance and breeding of new low-temperature-resistant varieties.