63 results about "Chilling injury" patented technology

A cold-resistant sprayer agent relates to a sprayer agent, which solves the problems of maturation period delay, output reduction and grain quality decline resulting from powder seeds under a low temperature condition, chilling injury death, difficult germination and delayed chilling injury in case of encountering the cold current in the seedling stage. The cold resistant sprayer agent is prepared by the weight percent as follows: 30%-80% of cold resistant nutriment, 4%-40% of antifreeze liquid, 0.02%-2.0% of surfactant, 0.001%-1.0% of plant growth regulator and water of the rest. The cold-resistant sprayer improves the cold resistance of the plant at a molecular level and is capable of reducing the mechanical damage formed by the ice crystal in the protoplasm at a low temperature.

The invention discloses a method for growing grapes. The method includes steps of (1), selecting varieties, preparing seedling lands and sufficiently applying base fertilizers; (2), enabling grape parent branches to sprout in advance, to be more specific, growing the grape parent branches, and applying chemicals to the grape parent branches to release the grape parent branches from natural dormant periods in advance; (3), allowing three films to cover each greenhouse, to be more specific, building the greenhouses, allowing each outer film with the thickness of 0.05mm to cover the corresponding greenhouse, allowing each inner film with the thickness of 0.03mm to cover the inside of the corresponding outer film, and allowing each mulching film to cover bed surfaces on two sides of the corresponding plant in the corresponding greenhouse in the same period; (4), regulating the temperatures and the humidity; (5), preventing and treating diseases of the grapes; (6), managing and bagging flowers and fruits, and spraying calcium chloride solution on the surfaces of the fruits in 15-20 days before the fruits are ripe; (7), allowing the grapes to be ripe and harvesting the grapes. The mass-volume concentration of the calcium chloride solution is 5-8%. The method for growing the grapes has the advantages that the natural dormant periods of the grapes can be completed in advance, accordingly, the grape parent branches can sprout in advance, the grapes can be ripe in advance, and chilling injury in early springs can be prevented; the grapes are excellent in quality, good in taste, strong in aroma and high in calcium content, are large and can be safely eaten.

The invention discloses a method for identifying the early-stage chilling tolerance of cucumis sativus L. The method is characterized in that the chilling tolerance of the cucumis sativus L is identified by means of measuring chlorophyll contents and chlorophyll fluorescent parameters Fo of leaves of seedlings of the cucumis sativus L. The method has the advantages that a growth index, physiological and biochemical indexes and the chlorophyll fluorescent parameter Fo of each material are measured under a low-temperature stress condition, correlation coefficients among the various indexes and chilling injury indexes of the materials are computed, the correlation coefficients among the chlorophyll contents and the chilling injury indexes and the correlation coefficients among the chlorophyll fluorescent parameters Fo and the chilling injury indexes are the highest, and accordingly the chlorophyll contents and the chlorophyll fluorescent parameters Fo can be directly measured by a chlorophyll content tester and a chlorophyll luminoscope, plants are prevented from being injured, the method is simple, operation is easy, and a basis can be provided for chilling-tolerance variety screening and breeding for production.

The invention discloses a pesticide residue degradation agent, relating to a disinfectant. The pesticide residue degradation agent consists of the following substances in parts by weight: 56 to 84 parts of water, 15 to 28 parts of amino acid, 1 to 5 parts of glutathione, 4 to 13 parts of beneficial microbial bacteria, 1 to 2.2 parts of borax, 0.6 to 2.4 parts of calcium sulfate, 0.2 to 0.8 part of magnesium sulfate, 0.5 to 1.7 parts of zinc sulfate, 4 to 9 parts of potassium sulfate, 0.2 to 0.7 part of frankincense, 0.2 to 0.5 part of radix bupleuri, 0.25 to 0.6 part of honeysuckle, 0.2 to 0.55 part of rhubarb, and 0.1 to 0.3 part of gentian. The pesticide residue degradation agent has the advantages of improvement of soil, continuous cropping resistance and strong stress resistance, significantly reduces the usage of chemical fertilizers and pesticides, greatly improves the quality and taste of agricultural products, achieves an obvious effect of increasing both production and income, removes pesticide injuries and herbicide residues, has a special effect on chilling injuries and freezing injuries, and can degrade poisonous gases.

Methods and compositions are provided for the introduction and washout of vitrifiable concentrations of cryoprotectant in organs and tissues. The methods comprise cooling the organ to below −10° C. by perfusion with a solution having a freezing point below −10° C., a temperature from −10 to −40° C., and a tonicity from 1.1 to 2.0 times isotonic, after previous perfusion with said solution for a time insufficient for approximate osmotic equilibration of the organ with the solution. The methods further comprise increasing the concentration of cryoprotectant further at a temperature from −10 to −40° C. to prepare the organ or tissue for vitrification. The methods further comprise cooling and vitrifying the organ, rewarming it, and perfusing the organ with a vitrifiable concentration of cryoprotectant that is the same as or less than the concentration used for vitrification, without the addition of an osmotic buffering agent. Rewarming is accomplished either by rapid (>1°C. / min, and preferably −0.2-20° C. / min) elevation of arterial perfusate temperature from below −20° C. to above −15° C. during continuous perfusion of the organ or by perfusing the organ with pre-warmed arterial perfusate at >−15° C. Extraordinarily effective multicomponent compositions are also provided for the process, particularly involving a vitrification solution whose warming rate after vitrification can be <1° C. / min without freezing during rewarming and a chilling injury protective solution having zero toxicity to whole organs at 0° C. and permitting almost complete avoidance of chilling injury at −20 to −25° C.

The invention belongs to the technical field of farm product preservation and particularly relates to a method for controlling the chilling injury of peaches and keeping the eating quality of the peaches. The method is characterized in that the properly matured peaches are picked and fumigated by ethanol combined with 1-MCP, then refrigerated and transported or sold. The first advantage of the method is good effect, i.e. the chilling injury of the peaches during the low-temperature storage can be obviously delayed and the freshness period can be prolonged; the second advantage is safety, i.e. the ethanol used in the method is a component naturally generated by plants such that the eating safety is high, only a little ethanol is used in the processing and the residual ethanol in the environment and on the surfaces of the peaches is eliminated by ventilation or other methods, such that the amount of the ethanol absorbed by the peaches is small, and the taste of the peaches remains the same after the processing, therefore the method has no food safety problems; the third advantage is easiness, i.e. the method is easy to operate and popularize and is suited to be used for mass production; and the fourth advantage is low cost.

The invention provides a cold-tolerance gene qCT-3-2HHZ for rice booting stage stable expression and a molecular marking method thereof, and belongs to the field of rice high-yield stress-tolerance breeding and molecular genetics. According to the linkage and linkage disequilibrium phenomenon, the Huang zhanhua variety and eight associated breeding populations derived from donor parents rich in diversity are used, a whole-genome relational analysis and physical mapping method is adopted, the cold-tolerance gene qCT-3-2HHZ for the rice booting stage stability expression is precisely positioned and verified, and a practical economical marker ZCT-7 based on common PCR is further developed. The cold-tolerance gene qCT-3-2HHZ and the molecular marking method are applied to rice chilling-injury-tolerance assisted selection and pyramid breeding, defects of natural variation genes in the prior art can be effectively made up for, negative effects caused by assistant breeding conducted through primary positioning results can be remarkably reduced, genotype selection can be carried out on low-generation breeding populations in the seedling stage, cold-tolerance individuals are obtained, hybridization and transfer are facilitated in time, the process of identifying low-temperature resistance in the adult-plant stage is omitted, breeding efficiency is improved, and the breeding process is accelerated.

A device is provided to be able to spray over a wide range and suppress a chilling injury. In the device, a rotating nozzle is connected to a connection pipe having a connection passage in communication to a nozzle stem of an aerosol can to have an outlet directed laterally. The rotating nozzle comprises a hollow support having a connection passage connected to a connection passage outlet of the connection pipe and a hollow arm rotatably supported at a tip end of the support, and an arm orifice is provided at a tip end of a connection passage in the arm and oriented such that reaction force of spray causes the rotating nozzle to rotate and aerosol contents are sprayed forwardly of a rotating plane of the rotating nozzle.

The invention relates to a novel seed coating for preventing and treating corn soil insect and resisting chilling injury in a seedling stage. The novel seed coating is characterized in that the prescription comprises the following raw materials by weight percent: 3-5% benfuracarb, 8% thiram, 0.6% tebuconazole and 0.01-0.05% of brassinolide. According to the effect of preventing and treating corn stem dwarf seedlings and the phenomena of no emerge seedlings or deformed seedlings caused by chilling injury in the seedling stage of corn, a certain dose of benfuracarb and brassinolide are added into the seed coating, so as to lower the incidence rate of corn stem dwarf seedlings, increase the emergence rate of corn seeds which cannot resist low temperature and tebuconazole hazard, and ensure the safety of agricultural production.

The invention discloses a method for preserving fruit. The method comprises the following steps: pre-treating the fruits, first refrigerating, second refrigerating, third refrigerating, aerating, sterilizing and completing. By using the method for preserving fruit, respiration and metabolism of the fruit can be effectively inhibited, so that ripening and aging of the fruit are postponed and quality of the fruit is maintained. According to the method, the fruit is pre-treated by gradual cooling, so that the fruit has a good adapting process to effectively avoid chilling injury caused by excessively low temperature of the refrigerator when the fruit enters the refrigerator for the first time; post-treatment of aerating is also utilized to suppress growth and metabolism speeds of the fruit, so that the fruit is effectively preserved. Innocent treatments are utilized in the whole preservation process of the method for preserving fruit, so that the method overcomes problems of multiple methods for preserving fruits, such as single preservation type, complex preservation method and impossible mixed preservation.

The invention discloses a breeding method for rapidly aggregating cold resistance and rice blast resistance of rice, relating to a rice breeding method. The invention aims to solve the problem of long breeding time in the existing cold-resistant and blast-resistant rice breeding methods. Methods: 1. Northeast japonica rice varieties are used as the female parent, and cold-resistant and rice blast-resistant japonica rice varieties are used as the male parent. F1 is obtained by crossing, and F2 is planted; 2. The field phenotype of F2 is initially selected, tested, and used as the F3 generation Three, the F3 generation is planted, tested and planted, as the F4 generation; Four, the F4 generation is planted, and the excellent strains are screened as the F5 generation; Five, the F5 generations are alternately planted, and the screened strains are the F6 generation; The cold-damaged rice area and rice blast disease-infested area are planted, and excellent strains are selected to obtain cold-resistant and rice blast-resistant rice. The method can lock target traits earlier, continuously track dominant traits and obtain rapid aggregation, advance and save the time for aggregation of ecological advantageous traits, and achieve early determination of breeding goals and early aggregation. For rice variety breeding.

Present invention provides an aqueous composition made from selected biochemical substances that will help with the low temperature tolerance of plants and thus to avoid “chilling injury” and to promote better recovery from such injury, when the solution is applied to crop plants prior to the arrival of cold snap or period of low temperature.

The invention discloses a cold-resistant anti-corrosion preservation technology for cold-sensitive fruits and vegetables after being harvested, and particularly relates to a cold-resistant preservative for cold-sensitive fruits and vegetables after being harvested and a preservation method thereof. The preservative disclosed by the invention comprises methyl jasmonate (2.0-10.0mg / L) and a mixed solution, wherein the mixed solution is an ice water solution containing salicylic acid, ascorbic acid, CaCl2 and an active agent Tween 80. The preservation method comprises the steps of firstly, carrying out sealed fumigation on the cold-sensitive fruits and vegetables for 12 hours by jasmonic acid methyl ester at room temperature, soaking and carrying out cold shock on the fruits and vegetables for 1 hour in the ice water mixed solution, taking out the fruits and vegetables and airing the surface water. The cold-resistant preservative does not generate obvious symptoms of chilling injury after being placed for two weeks in an environment at 0-4 DEG C; corruption mildew and quality drop of the fruits and vegetables stored in the environment at room temperature and proper low temperature can be effectively restrained; and the refreshing time is obviously prolonged.

The invention provides a controlled atmosphere storage and preservation method for flat peaches. The method includes the following steps that precooling is conducted, wherein flat peach fruits which are almost mature are picked and precooled at the temperature of 0-2 DEG C for 20-30 hours, and the fruit core temperature of the flat peach fruits is lowered to 0-2 DEG C; the precooled flat peach fruits are subjected to controlled atmosphere storage, according to the controlled atmosphere storage conditions, the volume concentration of oxygen is 3-8%, the volume concentration of carbon dioxide is 3-5%, temperature is 0-1 DEG C, and relative humidity is 85-90%. By means of the preservation method, the flat peach storage period can be effectively prolonged, the security freshness time of the flat peach fruits are prolonged to 20 days or above from 3 days at the normal temperature and 10-12 days of low temperature storage, chilling injury symptoms of fruit flesh browning, woolliness and the like and losing of flavor can be obviously delayed, high hardness, the content of soluble solids and malic acid and the content of citric acid of the flat peach fruits can be maintained, and the original solid acid ratio of the flat peach fruits is well maintained.

A hypobaric apparatus and methods capable of inducing and maintaining in stored horticultural cellular tissue controlled pervaporation of undesired chemical compounds without reaching the boiling point of intracellular water molecules, comprising a hypobaric chamber constructed to provide a leak rate of less than 4.0 mm Hg per hour, sensors and regulators coupled to the chamber and to a controller to measure and regulate target correlates. Commodities processed, including processing storage periods longer than any known involving controlled atmosphere, exhibit an absence of low oxygen injury, high carbon dioxide injury, chilling injury, leaf abscission, leaf de-greening, fungal decay, bacterial decay, gravitational curving, geotropic curvatures, leaf epinasty, stem epinasty, flower fading, senescence, live invertebrates at any life stage, fumigant chemical compounds, volatile fungicides, volatile bactericides; no advance in ripening; minimal water loss.

The present invention discloses a prunus salicina storage and preservation method. The prunus salicina fruits are soaked with calcium chloride solution at room temperature, naturally dried at room temperature, then subjected to an airtight fumigation of 1-methylcyclopropene, and then placed in a low temperature environment for cold storage. The prunus salicina storage and preservation method effectively reduces post-harvest respiration rate of the prunus salicina, reduces the decay rate of fruits, and combines with the calcium chloride solution to conduct soaking treatment, and maximumly preserves the hardness of the prunus salicina. Suitable temperature can inhibit microbial reproduction and chilling injury incidence, and the use of preservation bag for bagged storage also to reduce the respiration rate of the prunus salicina fruits. The calcium chloride and 1-methylcyclopropene are low in amount and free of toxic or side effects, and have relative high security and environmental protection. The used reagents and conditions are low in price and application cost. The operation process is simple, is easy to large-scale promotion, and has a high maneuverability.

Methods and compositions are provided for the cryopreservation of human organs and tissues. In certain embodiments, Step 1 comprises perfusion with a vitrifiable cryoprotectant solution at a temperature above −10° C. for a time insufficient for the approximate osmotic equilibration of the organ with the solution, followed by cooling the organ to below −10° C. by perfusion with said solution at a reduced temperature. In certain embodiments, Step 2 comprises increasing the concentration of cryoprotectant further at a temperature from −10 to −40° C. In certain embodiments, Step 3 comprises cooling and vitrifying the organ, rewarming it, and perfusing the organ with a vitrifiable concentration of cryoprotectant whose temperature is either raised gradually or is held at ≧−15° C. Compositions are provided that allow safe organ perfusion with vitrifiable media at >−10° C. and almost complete avoidance of chilling injury at −20 to −25° C. and that allow slow warming after vitrification without freezing.

The invention discloses a fruits and vegetables temperature control storage and transportation dedicated plastic pass box unit with hold-over plates for an insulating truck. The fruits and vegetables temperature control storage and transportation dedicated plastic pass box unit with the hold-over plates for the insulating truck includes multiple same box bodies and a box lid. Vertical concave slots or convex slots are formed in the box lid and the center positions of both sides of each box body. The box bodies are connected into a stacking in a front-and-back direction. The box lid and each box body are of plastic hollow grid structures. A layer of cold storage frame capable of containing the hold-over plates is arranged on the bottom surface of the box body, and the box lid is the hollow cold storage box which the hold-over plates can be put in. The cold storage box on the bottom surface of the last box body is embedded into the next box body, and the box body is fixed in an up-and-down direction. The box lid covers on the topside box body, the box lid and the box bodies are piled and sent into the insulating truck. The whole insulating truck is taken as an insulating unit, the hold-over plates are uniformly distributed inside the unit, due to the fact that the pass box has no insulating layer, air circulation is fluent inside the stacking so as to be beneficial to the uniform temperature distribution in the stacking, fruits and vegetables chilling injury caused by partial supercooling is prevented, and fruits and vegetables loading space is large; car utilization rate is high, the stacking is solid, and the temperature control effect is good.

A treatment method of seeds is provided which improves the capability of seed to resist imbibitional chilling injury, belonging to a field of treatment technology for crops, especially relating to treatment method of seeds. The process of the treatment method of seeds that improves the capability of seed to resist imbibitional chilling injury includes: quantitiy determined clean tobacco seeds is put into 10%PEG solution for treatment, according to a 1:10 (W / W) scale of clean dry tobacco seed to 10%PEG solution; the tobacco seeds are soaked for 72 hours continuously in the condition of temperature, 15+ / -2 DEG C, and ventilation for oxygen complement; the soak solution is removed after 72 hours, then washing and air drying the seeds; subsequently, the tobacco seeds are coated conventionally and sealed to be sowed. The method improves the capability of seed to resist imbibitional chilling injury significantly, which ensures the seeds to have a high germination rate, prior seedling formation stage and high seedling quality after being assaulted by low temperature to improve agronomic traits of tobacco transplanted to field, and yield and output value of tobacco.

The invention relates to a fruit and vegetable fresh-keeping method, a fruit and vegetable fresh-keeping agent and an application. The fruit and vegetable fresh-keeping method comprises treating fruits and vegetables to be refrigerated by using the fresh-keeping agent, wherein the fresh-keeping agent contains arachidonic acid. The fruit and vegetable fresh-keeping method, the fruit and vegetable fresh-keeping agent or the application provided by the invention relates to arachidonic acid, and has the effects of obviously reducing chilling injury indexes of the fruits and vegetables, inhibitingthe increase of cell membrane permeability of the fruits and vegetables and the accumulation of malondialdehyde, relieving the occurrence of chilling injuries of the fruits and vegetables, delaying the decline of the content of soluble solids in the fruits and vegetables, inhibiting the increase of the content of titratable acid, and thus improving the quality of the fruits and vegetables under low-temperature storage.

The invention discloses a refrigeration method of high quality celery. The method is characterized by comprising the following steps: (1) precooling treatment of celery, (2) antistaling agent preparation, (3) precooling, (4) packaging by a food grade polyethylene film, and (5) stagewise cooling refrigeration storage. Through addition of the antistaling agent and stagewise cooling in storage, the celery can slowly adapt to the increasingly lower temperature and gradually reduce the sensitivity to low temperature, so as to greatly reduce the probability of chilling injury of the celery, keep the advantages of crisp roots, tender stems and sufficient moisture of the celery, maintain the taste and quality of celery, and extend the preservation time.

This invention relates generally to cryopreservation and a method for preventing injury caused by cooling and warming of tissue and for reducing the toxicity of vitrification solutions. The present method achieves reduction or elimination of injury by increasing the tonicity of the medium to greater than isotonic prior to cooling. The method was developed by attempting to simulate without freezing, the events that take place during freezing living cells and / or tissue. A further benefit of the method is that, since the cryopreservation medium is hypertonic, it can be diluted to a more extreme degree in one step once the system is rewarmed, without engendering the degree of cell swelling that would attend the same dilution factor when diluting an isotonic cryopreservation medium.

The invention relates to the technical field of fruit ripening and fresh-keeping after ripening, in particular to a production method of instant fruits. The method comprises the steps as follows: picking, sorting, ultrasonic assisted chilling injury treatment, rewarming, ultrasonic assisted chilling injury treatment, preservative treatment, rewarming, decompression and mixed gas treatment; and theultrasonic assisted chilling injury treatment is to spray a loquat / rose compound emulsion with a mass fraction of 0.07%-0.1% into a refrigeration environment by use of an ultrasonic humidifier duringthe refrigeration process, and the fruits are treated with ultrasonic waves at a frequency of 40-50 kHz in the process of chilling injury treatment. Kiwi fruits, passion fruits, pitaya, apples and bananas treated by the method can be eaten instantly and are not required to be placed naturally for a long time for ripening, the storage, transportation and shelf life of the instant fruits can be further prolonged, and at the same time, the quality of the fruits is maximally maintained.

The invention relates to a treatment method for improving the imbitional chilling injury resistance of plant seeds. The treatment method comprises the following steps: preparing normal butanol with deionized water, diluting until the concentration of the normal butanol is 0.01-0.08%, performing the imbitional chilling injury treatment on plant seeds, and performing external source addition treatment during germination. The invention belongs to the field of a crops treatment technology and in particular relates to a seed treatment method. Sensitive seeds are externally added with butyl alcohol or N-acyl ethanol in an imbitional chilling injury process at 4 DEG C, the tolerance of the sensitive seeds in an imbitional chilling injury process is improved remarkably, the crop seeds still have the high germination rate, relatively short seedling maturing period and high mature seedling quality, the yield of the crops is increased, and the method has the good application prospect.

The invention relates to a maize lodging-resisting stress-resisting yield-increasing regulator, and a preparation method and application thereof. The regulator contains the following components: iminodisuccinic acid 140-280 g / L, ethephon 90-180 g / L, zinc sulfate heptahydrate 50-100 g / L, active agent also used as a spreading agent 5-10 g / L, and the balance of water. The active agent also used as the spreading agent is selected from one or more of Tween 20 and Tween 60. The regulator of the invention can significantly enhance the ability of maize such as resisting low-temperature chilling injury, high temperature, drought and mild saline-alkali stress, increase the lodging resistance of maize, increase the number of ears per mu, grain number per ear and thousand-grain weight, and increase the maize yield by 20% or more. At the same time, the regulator has the characteristics of low cost, convenient use, and less residues in the field. The regulator is easy to popularize and apply, and has a positive role in promoting China corn production.

The invention discloses an application of GB in promoting turfgrass revival and prolonging green period. The invention discovers for the first time that the quality of turf where GB is sprayed under chilling injury stress and low-temperature domestication is remarkably higher than that of control. Agrostis stolonifera is transferred to an environment lower than 10 DEG C from a freeze injury environment, the green turf of a material where GB is sprayed is remarkably improved as compared with a material without GB spraying, and revival is brought forward.

The invention belongs to the technical field of crop planting and particularly relates to a microenvironment fine temperature-regulation banana winter warming method. The microenvironment fine temperature-regulation banana winter warming method comprises bagging bananas during a budding stage, performing electric or fire heating inside bags to avoid low-temperature damage to banana skins and ensure normal ripening acceleration and bright yellow color of the bananas, and meanwhile, replenishing biological fertilizers to improve cold resistance of plants.

The embodiment of the invention discloses a cultivation method for preventing chilling injury of rice. The method comprises the following steps: applying a base fertilizer before rice transplanting, applying a tillering fertilizer after rice transplanting, and applying a panicle fertilizer when the rice grows branches and panicles, wherein, during the rice planting process, 70%-80% of the total nitrogen fertilizer amount is applied as the base fertilizer and the tillering fertilizer, 20%-30% of the total nitrogen fertilizer amount is applied as the panicle fertilizer, and the total nitrogen fertilizer application amount is reduced by 20%-30% compared with the normal rice planting process. In the cultivation method for preventing chilling injury of rice of the embodiment of the invention, amethod of applying moderate nitrogen and high phosphorus and potassium in fertilization of a rice field is adopted, that is, the total nitrogen fertilizer application amount is appropriately controlled, a fast-acting nitrogen fertilizer is less applied, and a phosphorus and potassium fertilizer is more applied, so that cold resistance of rice seedlings is improved, and the disease is not caused due to poor absorption of phosphorus and potassium after the rice seedlings are transplanted in cold-soaked fields. The microenvironment of rice fields is improved, and thereby the bio-physiological characteristic of rice and the yield of rice are improved.

The invention discloses a method for managing and curing flue-cured tobacco to alleviate chilling injury of tobacco based on salicylic acid application. The flue-cured tobacco management method comprises the following steps: 1) prevention of chilling injury of tobacco: according to weather conditions, spraying salicylic acid solution to prevent chilling injury of tobacco plant which has not occurred chilling injury; 2) grade identification and primary spray of chilling injury tobacco: grade identification of chilling injury tobacco plant is carried out, and that disease degree of chilling injury tobacco is divide into three grades; And spraying once according to the grade of chilling injury tobacco; 3) re-identification and secondary spray of chilling injury tobacco grade: firstly, re-identification and secondary spray of chilling injury tobacco grade are carried out on that tobacco plant 10-20 days aft the primary spraying, and the chilling injury tobacco grade is not more than 1 grade, so that the chilling injury tobacco grade is not more than 1 grade. The invention sprays salicylic acid solution with suitable concentration to alleviate the damage degree according to the identification of chilling injury tobacco of different damage grades, and solves the problem of occurrence of field chilling injury tobacco caused by factors such as sudden drop of ambient temperature, too low temperature, too high soil humidity, too low soil temperature and the like in the field.